Abstract：
:The magnetic cell sorter (MACS) technique was applied to isolate retinal ganglion cells (RGCs) for culture. RGCs were labeled retrogradely with 1.1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (Dil). Subsequently retinal cell suspensions were incubated with biotinylated anti-rat Thy-1 antibody and MACS Streptavidin MicroBeads, and then applied onto the column in the magnetic fields. Cells attached on the column were flashed out without magnetism and plated on glass cover slips. RGCs were enriched to 31.0% of all cells with MACS from 0.55% before applying onto the magnetic column. Mean diameters of Dil-labeled cells were significantly larger than those of unlabeled cells. All cells with soma diameter over 11 microm were labeled. The number of viable RGCs were counted in the 10 fields of six cultures at a magnification of x200; the mean numbers on the 2nd, 7th and 14th culture-day were 53+/-3, 24+/-2 and 21+/-3, respectively (mean +/- SEM, n = 6). Thus, the MACS technique was confirmed to be useful for enrichment of RGCs and long-term study of cultured RGCs.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Shoge K,Mishima HK,Mukai S,Shinya M,Ishihara K,Kanno M,Sasa Mdoi
10.1016/s0304-3940(98)00918-5keywords:
subject
Has Abstractpub_date
1999-01-08 00:00:00pages
111-4issue
2eissn
0304-3940issn
1872-7972pii
S0304-3940(98)00918-5journal_volume
259pub_type
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