Abstract:
:To better understand the pathophysiological role of Src protein, a non-receptor protein tyrosine kinase of 60kDa, in the ischemic brain, we investigated the time course and regional distribution of active Src expression by using a specific antibody against Tyr416 phosphorylated Src (phospho-Src) in the rat hippocampus after transient forebrain ischemia. In the hippocampus of the control animals, active Src expression was too low to be detected by immunolabeling. Beginning 4h after reperfusion, active Src expression became evident and, after 1 day, had increased preferentially in the CA field of the hippocampus proper and the dentate gyrus. By day 3, active Src expression markedly increased in the pyramidal cell layer of CA1 and the dentate hilar region in temporal correlation with neuronal cell death occurring in these areas, where cells typical of phagocytic microglia showed phospho-Src immunoreactivity. Double-labeling experiments revealed that cells expressing active Src were microglia that stained for biotinylated lectin derived from Griffonia simplicifolia (GSI-B4). Active Src expression began to decline at day 7 and returned to the basal level by day 14 after reperfusion. These results demonstrate increased phosphorylation of Src in activated microglia of the post-ischemic hippocampus, indicating that Src signaling may be involved in the microglial reaction to an ischemic insult.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Choi JS,Kim HY,Chung JW,Chun MH,Kim SY,Yoon SH,Lee MYdoi
10.1016/j.neulet.2005.01.015keywords:
subject
Has Abstractpub_date
2005-05-20 00:00:00pages
1-5issue
1-2eissn
0304-3940issn
1872-7972pii
S0304-3940(05)00029-7journal_volume
380pub_type
杂志文章abstract::The activities of monoamine oxidase (MAO)-A and -B in subcellular fractions of bovine retina were determined using serotonin and beta-phenylethylamine, respectively, as substrates. The subcellular fractions were a P1 fraction containing photoreceptor cell synaptosomes and a P2 fraction enriched with small synaptosomes...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(81)90382-7
更新日期:1981-01-20 00:00:00
abstract::Brain content of leucine-enkephalin and methionine-enkephalin changes independently during kindling of the amygdala. Both peptides were measured after 4, 8, 15 and 21 days of stimulation. Leu-enkephalin showed a progressive increase during the kindling: the results on the 4th day did not differ significantly from the ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(81)90337-2
更新日期:1981-10-23 00:00:00
abstract::Frequent and persistent stressful events caused depressive illness. Stress is an aversive stimulus which disturbs physiological homeostasis and reflects a variety of biological systems. The present study was designed to investigate the nitric oxide mechanism in the protective effect of imipramine and venlafaxine again...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2009.10.004
更新日期:2009-12-25 00:00:00
abstract::In vivo formation of 1-methyl-4-phenylpyridinium ion (MPP(+)) in the striatum, and dopaminergic neurotoxicity following systemic administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in the presence and absence of calcium channel agonist (+/-)-Bay K8644 were analyzed in Balb/c mice. We used HPLC-photod...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(03)00577-9
更新日期:2003-07-31 00:00:00
abstract::We investigated the effects of aging on the neuroanatomical sex dimorphisms of the rat hypothalamic ventromedial nucleus (VMN). Stereological methods were used to estimate the volume of the VMN and the total number and size of its neurons, including their dendritic trees, in males and females aged 6 and 24 months. No ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(01)02294-7
更新日期:2001-11-13 00:00:00
abstract::After systemic administration of the dopamine agonist apomorphine (APO) in common toads Bufo bufo spinosus, the excitatory receptive fields of retinal R2 and R3 ganglion cells doubled their size and the response activities of these neurons to moving visual stimuli were generally increased. Comparable investigations of...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(94)90155-4
更新日期:1994-05-23 00:00:00
abstract::The binding site for 1,4-benzodiazepines in the brain is part of the hetero-oligomeric gamma-aminobutyric acid (GABA)A receptor complex which regulates a chloride ion channel. The presence of the gamma 2 subunit in the complex is necessary for the binding of benzodiazepines to their binding site. This study demonstrat...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(95)12217-6
更新日期:1995-12-29 00:00:00
abstract::AMP-activated protein kinase (AMPK) was recently suggested to have a pro-apoptotic effect although its primary function is believed to mediate cellular adaptation to metabolic stresses. Here, we investigated the effect of the AMPK activator 5-aminoimidazole-4-carboxamide-ribonucleoside (AICAR) on oxidative stress-indu...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2003.10.012
更新日期:2004-01-16 00:00:00
abstract::The co-localization of neuropeptide tyrosine (NPY) and somatostatin (SOM) in rat hippocampal cells was studied in double labelling experiments using a combination of antibodies against the two peptides on the same tissue section. The individual hippocampal subfields show large variations in the relative number of NPY-...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(87)90551-9
更新日期:1987-07-09 00:00:00
abstract::myo-Inositol monophosphatase (E.C.3.1.3.25) catalyzes the hydrolysis of myo-inositol 1-phosphate in the presence of Mg2+ at a physiologic pH to form free myo-inositol, maintaining a supply that represents the precursor for inositol phospholipid second messenger signaling systems. In the present study the activity and ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(98)00209-2
更新日期:1998-04-10 00:00:00
abstract::Excessive activation of the N-methyl-D-aspartate (NMDA) receptor-channel complex has been implicated as one of the mechanisms by which ischemia-induced neuronal damage is mediated. Elevated glycine levels during ischemia may contribute to damage mediated by the NMDA receptor as glycine binding potentiates NMDA respons...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(92)90191-9
更新日期:1992-09-28 00:00:00
abstract::This article has been withdrawn at the request of the Editor-in-Chief. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at https://www.elsevier.com/about/our-business/policies/article-withdrawal. ...
journal_title:Neuroscience letters
pub_type: 撤回出版物
doi:10.1016/j.neulet.2020.135214
更新日期:2020-06-29 00:00:00
abstract::Recent imaging studies have reported the projection of semicircular canal signals onto wide regions of the cerebral cortex but little is known about otolith projections onto the cerebral cortex. We used functional magnetic resonance imaging (fMRI) to investigate the activation of the cortex by loud clicks that selecti...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2007.06.036
更新日期:2007-08-09 00:00:00
abstract::A new non-N-methyl-D-aspartate (non-NMDA) glutamate receptor antagonist, GYKI 52466, was tested on L-glutamate (Glu)-, kainate (KAI)- and NMDA-induced responses in vivo, using both extracellular recording of antidromic field potentials and intracellular recording from rat abducens motoneurones. Intravenous (5-10 mg/kg...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(91)90115-a
更新日期:1991-04-15 00:00:00
abstract::Intracellular recording techniques were used to monitor the evoked responses in hippocampal CA1 pyramidal cells following focal stimulation of the proximally and distally located apical dendritic synapses. The local anaesthetic, QX-314, when injected into individual cells, caused a complete time-dependent blockade of ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(94)90665-3
更新日期:1994-05-19 00:00:00
abstract::Discrete brain structures were analysed for gamma-aminobutyric acid (GABA) and vasopressin content in normo- and hypotensive rats treated with the glutamic acid decarboxylase inhibitor, 3-mercaptopropionic acid (MPA) and the GABAA agonist muscimol. In the normotensive group treated with MPA only, the concentration of ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(89)90546-6
更新日期:1989-07-03 00:00:00
abstract::In the present study, newborn male Wistar rats were injected, subcutaneously, five times, every other day, with monosodium glutamate (MSG, 4 g/kg bw) or saline (as control, C), during the neonatal period. MSG animals developed destruction of the arcuate nuclei (ARC) with absence of NPY-immunoreactive cell bodies, whic...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2005.12.043
更新日期:2006-05-01 00:00:00
abstract::In untreated patients with Parkinson's disease (PD), the total (free and conjugated) serotonin (5-HT) and dopamine (DA) concentrations in the cerebrospinal fluid decreased significantly. While the 5-HT concentration displayed a non-significant trend of negative correlation with the DA concentration in controls, it had...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(93)90817-5
更新日期:1993-09-03 00:00:00
abstract::The atypical serine/threonine protein kinase, a mammalian target of rapamycin (mTOR), is believed to be essential to the regulation of cell growth and the functions of the central nervous system. By using calcium imaging and patch-clamping techniques to study the role of this signaling pathway in the activity of cultu...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2008.06.006
更新日期:2008-08-15 00:00:00
abstract::Delta9-THC and synthetic cannabinoids produce memory impairment in humans as well as in laboratory animals. The high concentration of cannabinoid CB1 receptors and the presence of endocannabinoids in the hippocampus suggest that a cannabinoid neurochemical system may play a role in learning and memory processes. Thus,...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2005.01.049
更新日期:2005-06-03 00:00:00
abstract::Tritiated dipyridamole, a specific adenosine uptake inhibitor binds in a saturable and reversible fashion to high-affinity receptor sites in guinea pig brain sections (Kd = 10 +/- 1.5 nM; Bmax = 650 +/- 100 fmol/mg prot.). The anatomical distribution of [3H]dipyridamole binding sites obtained with autoradiographic tec...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(86)90043-1
更新日期:1986-05-23 00:00:00
abstract::Secondary optic projections were studied in the toad, Bufo marinus using the method of transneuronal transport of triated proline and fucose. Following a survival of 14 days after eye injection autoradiographic label was transported transneuronally to the contralateral nucleus isthmi, the deeper layers of the contrala...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(80)90004-x
更新日期:1980-03-01 00:00:00
abstract::Growth/differentiation factor-5 (GDF-5), a member of the transforming growth factor-beta (TGF-β) superfamily, has been shown to protect rat dopaminergic neurons against insult both in embryonic neuronal culture and in Parkinson's disease models. However, whether GDF-5 exerts neuroprotective effects in hippocampal neur...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2017.04.055
更新日期:2017-06-09 00:00:00
abstract::Whole-cell recordings were obtained from respiratory neurons by applying patch-clamp techniques in the en bloc medulla of in vitro neonatal rat brainstem-spinal cord preparations. Stable voltage-clamp recordings of excitatory or inhibitory synaptic drive currents and current-clamp recordings of spike discharge of insp...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(92)90504-z
更新日期:1992-01-06 00:00:00
abstract::Alzheimer's disease (AD) brains contain high levels of advanced glycation endproducts (AGEs). Double immunostaining using anti-AGE and anti-apolipoprotein E (apoE) antibodies demonstrated that AGEs co-localized to a very high degree with apoE. We examined the binding of apoE to in vitro-prepared AGE-bovine serum album...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(97)00266-8
更新日期:1997-05-02 00:00:00
abstract::Hydrogen peroxide (H(2)O(2)) is a major reactive oxygen species that has been implicated in various neurodegenerative diseases. Quercetin, one of the plant flavonoids, has been reported to harbor various physiological properties including antioxidant activity. In this study, we investigated the neuroprotective effects...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2011.09.028
更新日期:2011-10-31 00:00:00
abstract::Nitric oxide synthase (NOS) immunoreactivity occurred in about 60% of ventromedial, ventrolateral, and sphincteric motoneurones in cat sacral spinal cord. Proportions of sacral motoneurones histochemically stained for NADPH-diaphorase, were similar, and equivalent in size to those immunoreactive for NOS, suggesting co...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(95)11831-g
更新日期:1995-08-18 00:00:00
abstract::The purpose of this study was to investigate how the recruitment gain, as derived from the Hoffmann (H)-reflex measurement, of antagonistic motoneurons is modulated during voluntary lengthening (LEN) and shortening (SHO) contractions in the human tibialis anterior (TA) muscle. To this end, the parameters of the ratios...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(03)00241-6
更新日期:2003-05-15 00:00:00
abstract::Vesicular monoamine transporter 2 (VMAT2) is responsible for packing dopamine into vesicles, and reduces the effects of neurotoxins by sequestering them into vesicles. In this report, we tested the hypothesis that VMAT2 is associated with Lewy body (LB) formation by immunohistochemical staining of midbrain and cortica...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2005.11.068
更新日期:2006-04-03 00:00:00
abstract::We examined the effect of intraventricular injection of nerve growth factor (NGF) on the choline acetyltransferase (ChAT) and vesicular acetylcholine transporter (VAChT) mRNA expression in the septa of neonatal rats. Rat pups were injected with 2.5 S NGF or cytochrome-c (control) on postnatal days (PN) 4 and 18, and s...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(96)12629-x
更新日期:1996-05-10 00:00:00