Hypoxia and reoxygenation increased BACE1 mRNA and protein levels in human neuroblastoma SH-SY5Y cells.

Abstract:

:Ischemic cerebrovascular diseases, usually involved in hypoxia and reoxygenation, have been reported to increase the risk of dementia such as Alzheimer's disease (AD). beta-site amyloid protein precursor (APP)-cleaving enzymes (BACE1) have been identified to participate in the secretion of beta-amyloid peptides (Abeta), and its expressive alteration would contribute to the AD neuropathology. We have investigated the effect of hypoxia (0% O(2), 24h) and reoxygenation (0h, 12h and 24h after 24h hypoxia) on BACE1 mRNA and protein levels in human neuroblastoma SH-SY5Y cells. At the same time, we also examined the effect of hypoxia and reoxygenation on APP mRNA and protein levels. We demonstrated that hypoxia and reoxygenation did not alter APP mRNA and protein level, However compared to those of controls, BACE1 mRNA levels were up-regulated by 31.5% (P=0.028) and 35.1% (P=0.005) at 12h and 24h and the protein levels increased to 22%(P=0.021), 42% (P=0.000) and 51.5% (P=0.000) at 0h, 12h and 24h after reoxygenation, respectively. Thus by up-regulating of BACE1 mRNA and protein level in the neuronal cell, hypoxia may be a linkage in the pathophysiology between cerebravascular diseases and AD.

journal_name

Neurosci Lett

journal_title

Neuroscience letters

authors

Xue S,Jia L,Jia J

doi

10.1016/j.neulet.2006.07.013

subject

Has Abstract

pub_date

2006-09-25 00:00:00

pages

231-5

issue

3

eissn

0304-3940

issn

1872-7972

pii

S0304-3940(06)00680-X

journal_volume

405

pub_type

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