Bacterial growth and toxin production in ileostomy effluents.


:Escherichia coli (2), Vibrio cholerae (2) and Aeromonas sobria (1) strains were examined for their ability to grow and produce toxins in samples of ileostomy fluid. Three categories of response were observed: no detectable growth, growth without detectable toxin, and growth with detectable toxin. Clear differences were apparent between samples of ileostomy fluid obtained from different individuals and between samples obtained from the same individual at different times. The patterns of response were unique for each of the five test strains. We propose that the procedure developed forms a basis for investigating the host-parasite relationship in diarrhoeal disease.


J Med Microbiol


Ala Aldeen DA,Barer MR




Has Abstract


1989-04-01 00:00:00












  • Mechanisms of chloramphenicol resistance in Haemophilus influenzae in the United Kingdom.

    abstract::Of 2458 isolates of Haemophilus influenzae examined in a recent British survey, 42 were resistant to chloramphenicol. Two resistant isolates were of type b and 40 were non-capsulate. Spectrophotometric assay showed that all the resistant isolates produced chloramphenicol acetyltransferase (CAT). CAT activity did not i...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Powell M,Livermore DM

    更新日期:1988-10-01 00:00:00

  • Radioimmunoassay of herpes-simplex and measles virus antibodies in serum and cerebrospinal fluid of patients without infectious or demyelinating diseases of the central nervous system.

    abstract::A solid-phase radioimmunoassay was used to detect IgG antibodies against herpes-simplex virus antigens (capsid, envelope and excreted) and against measles virus antigen in serum and cerebrospinal fluid (CSF) specimens of 61 patients with no evidence of infectious or demyelinating disease of the central nervous system....

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Kalimo KO,Marttila RJ,Ziola BR,Matikainen MT,Panelius M

    更新日期:1977-11-01 00:00:00

  • Effects of mucopolysaccharides on penicillin-induced lysis of Staphylococcus aureus.

    abstract::Effects of four mucopolysaccharides and dextran sulphate on penicillin-induced lysis of Staphylococcus aureus FDA 209P were studied. Heparin and dextran sulphate inhibited lysis, whereas hyaluronic acid enhanced it. Chondroitin sulphates A and C had no effect. Incubation of S. aureus suspended in 0.03 M phosphate buff...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Kiriyama T,Miyake Y,Sugai M,Kobayashi K,Yoshiga K,Takada K,Suginaka H

    更新日期:1987-12-01 00:00:00

  • Role of biofilm morphology, matrix content and surface hydrophobicity in the biofilm-forming capacity of various Candida species.

    abstract::The present study aimed to evaluate the role of biofilm morphology, matrix content and surface hydrophobicity in the biofilm-forming capacity of Candida albicans and non-albicans Candida (NAC) spp. Biofilm formation was determined by microtitre plate assay and bright-field and scanning electron microscopy. The matrix ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Kumari A,Mankotia S,Chaubey B,Luthra M,Singh R

    更新日期:2018-06-01 00:00:00

  • The spa typing of methicillin-resistant Staphylococcus aureus isolates by High Resolution Melting (HRM) analysis.

    abstract::Molecular typing is an important tool for control and prevention of infection. A suitable molecular typing method for epidemiological investigation must be easy to perform, highly reproducible, inexpensive, rapid and easy to interpret. In this study, two molecular typing methods including the conventional PCR-sequenci...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Fasihi Y,Fooladi S,Mohammadi MA,Emaneini M,Kalantar-Neyestanaki D

    更新日期:2017-09-01 00:00:00

  • Disseminated intravascular coagulation in rabbits: synergistic activity of meningococcal endotoxin and materials egested from leucocytes containing meningococci.

    abstract::Leucocyte-egested material was harvested after the quantitative in-vitro phagocytosis of Neisseria meningitidis by rabbit or mouse polymorphonuclear leucocytes. The egested material was injected subcutaneously into rabbits and followed 24 h later with an intravenous injection of what would by itself have been a non-le...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: DeVoe IW,Gilka F

    更新日期:1976-11-01 00:00:00

  • Purification and characterisation of a metallopeptidase of Candida albicans.

    abstract::A novel aminopeptidase was purified by high performance liquid chromatography from a cytosoluble 100,000 g extract of Candida albicans on the basis of its ability to cleave L-arginine 7-amino-4-methylcoumarin. The purification factor was 36 and the yield was 20%. The native enzyme had a mol. wt of 52 kDa as demonstrat...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: el Moudni B,Rodier MH,Barrault C,Ghazali M,Jacquemin JL

    更新日期:1995-10-01 00:00:00

  • Expression of prostaglandin receptors in Chlamydia trachomatis-infected recurrent spontaneous aborters.

    abstract::A study was undertaken to quantify the expression of prostaglandin (PG) receptors and find the effect of gestational age on expression of PG receptor genes in Chlamydia trachomatis-infected recurrent spontaneous aborters (RSA). Endometrial curettage tissue (ECT) was collected from 130 RSA (Group I) and 100 age-matched...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Singh N,Prasad P,Singh LC,Das B,Rastogi S

    更新日期:2016-06-01 00:00:00

  • Identification of mutants with altered phenazine production in Pseudomonas aeruginosa.

    abstract::Pseudomonas aeruginosa is an opportunistic human pathogen that causes serious and chronic infections. Many secondary metabolites are secreted throughout its growth, among which phenazine is a known virulence factor and signalling molecule. Phenazine is coordinately controlled by the global regulatory quorum-sensing (Q...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Liang H,Duan J,Sibley CD,Surette MG,Duan K

    更新日期:2011-01-01 00:00:00

  • Competition for glucose between Candida albicans and oral bacteria grown in mixed culture in a chemostat.

    abstract::The competition for glucose as a growth-limiting substrate between Candida albicans and a mixed community of oral bacteria was investigated. A chemostat was operated under glucose-limiting and glucose excess conditions at a dilution rate of 0.05/h. A mixed population of oral bacteria was established and after a steady...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Basson NJ

    更新日期:2000-11-01 00:00:00

  • Bacillus cereus food poisoning: a provisional serotyping scheme.

    abstract::A provisional serotyping scheme for Bacillus cereus has been developed. Eighteen selected cultures of B. cereus isolated from foods and clinical specimens were used to prepare agglutinating sera against the flagellar antigen. There were no significant cross-reactions between the sera. The sera were used to type 137 is...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Taylor AJ,Gilbert RJ

    更新日期:1975-11-01 00:00:00

  • Comparative study of isolates from community-acquired and catheter-associated urinary tract infections with reference to biofilm-producing property, antibiotic sensitivity and multi-drug resistance.

    abstract:PURPOSE:Urinary tract infection (UTI) can be community-acquired (Com-UTI) or catheter-associated (CAUTI) and may be associated with biofilm-producing organisms. A comparative analysis of biofilm-producing property (BPP), antibiotic-sensitivity and multi-drug resistance (MDR) and their relation with the BPP of isolates ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Bardoloi V,Yogeesha Babu KV

    更新日期:2017-07-01 00:00:00

  • A clinical isolate of Escherichia coli co-harbouring mcr-1 and blaNDM-5 in Japan.

    abstract::The mcr-1 gene encodes a phosphoethanolamine transferase, which confers resistance to colistin by transferring phosphoethanolamine to lipid A. This study describes the emergence of a colistin- and carbapenem-resistant clinical isolate of Escherichia coli harbouring mcr-1 and blaNDM-5 genes, located on 90 and 150 Kb pl...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Uchida H,Tada T,Sugahara Y,Kato A,Miyairi I,Kirikae T

    更新日期:2018-08-01 00:00:00

  • Opsonic requirements of Helicobacter pylori.

    abstract::The opsonic requirements of Helicobacter pylori were investigated in a series of experiments with human polymorphonuclear leucocytes (PMNL). Pre-incubation of H. pylori with pooled normal human serum (NHS) in concentrations of 5-20% significantly increased the uptake of radiolabelled bacteria by PMNL. Treatment of the...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: McKinlay AW,Young A,Russell RI,Gemmell CG

    更新日期:1993-03-01 00:00:00

  • The classical Bordetella species and MALDI-TOF technology: a brief experience.

    abstract:PURPOSE:The aim of this work was to evaluate and optimize the identification of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica (usually known as the classical Bordetella species) using Bruker Biotyper matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF M...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Zintgraff J,Irazu L,Lara CS,Rodriguez M,Santos M

    更新日期:2018-12-01 00:00:00

  • Induction of granulomas in interferon-gamma gene-disrupted mice by avirulent but not by virulent strains of Mycobacterium tuberculosis.

    abstract::To gain a better understanding of the pathological role of interferon-gamma (IFN-gamma) in specific granuloma formation, IFN-gamma gene-deficient mice (BALB/c and C57BL/6) were produced. The IFN-gamma gene in embryonic stem (ES) cells was disrupted by inserting the beta-galactosidase gene (lacZ) and the neomycin resis...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Sugawara I,Yamada H,Kazumi Y,Doi N,Otomo K,Aoki T,Mizuno S,Udagawa T,Tagawa Y,Iwakura Y

    更新日期:1998-10-01 00:00:00

  • New assay for Gardnerella vaginalis loads correlates with Nugent scores and has potential in the diagnosis of bacterial vaginosis.

    abstract::Gardnerella vaginalis is a Gram-variable anaerobic bacterium present in 100% of women with bacterial vaginosis (BV). BV is a complex polymicrobial condition with no single causative agent. The current laboratory detection method for BV relies on a Gram-stain Nugent score to estimate the quantity of different bacterial...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Cox C,McKenna JP,Watt AP,Coyle PV

    更新日期:2015-09-01 00:00:00

  • Virulence determinants in clinical Staphylococcus aureus from monomicrobial and polymicrobial infections of diabetic foot ulcers.

    abstract::Antibiotic resistance in Staphylococcus aureus is a major public health concern, and methicillin-resistant S. aureus has emerged as an important pathogen. We characterized S. aureus isolates from monomicrobial and polymicrobial wound infections from 200 diabetic individuals with foot ulcers to understand their underly...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Shettigar K,Jain S,Bhat DV,Acharya R,Ramachandra L,Satyamoorthy K,Murali TS

    更新日期:2016-12-01 00:00:00

  • Haitian-like genetic traits with creeping MIC of Azithromycin in Vibrio cholerae O1 isolates from Puducherry, India.

    abstract::Introduction. The emergence of novel strains of Vibrio cholerae O1 El Tor biotype has gained attention due to causing several epidemics around the world. Variant strains have evolved as a result of the acquisition of genes that confer extended virulence and pathogenicity.Aim. This study aimed to determine the presence...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Mohanraj RS,Samanta P,Mukhopadhyay AK,Mandal J

    更新日期:2020-03-01 00:00:00

  • The value of LYM-1 cells for examining vacuole formation and loss of cell viability induced by culture supernates of Helicobacter pylori.

    abstract::Some strains of Helicobacter pylori are known to produce an extracellular cytotoxin that causes vacuolation in cultured mammalian cells. Screening for such strains makes use of HeLa cells which may not be sensitive enough to detect minimal changes. The aim of this study was to develop a more sensitive cell line. Vacuo...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Wang XM,Kojima T,Satoh K,Taniguchi Y,Tokumaru K,Saifuku K,Seki M,Kihira K,Ido K,Uchida JY,Ohmori C,Takaoka T,Kimura K

    更新日期:1997-08-01 00:00:00

  • A simple blood-culture bacterial pellet preparation for faster accurate direct bacterial identification and antibiotic susceptibility testing with the VITEK 2 system.

    abstract::An ammonium chloride procedure was used to prepare a bacterial pellet from positive blood cultures, which was used for direct inoculation of VITEK 2 cards. Correct identification reached 99% for Enterobacteriaceae and 74% for staphylococci. For antibiotic susceptibility testing, very major and major errors were 0.1 an...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Prod'hom G,Durussel C,Greub G

    更新日期:2013-05-01 00:00:00

  • Inhibitory efficacy of geraniol on biofilm formation and development of adaptive resistance in Staphylococcus epidermidis RP62A.

    abstract:PURPOSE:The current study has been designed to delineate the efficacy of geraniol (GE) on biofilm formation in Staphylococcus epidermidis as well as the effect of subinhibitory concentrations of GE on the development of adaptive resistance. METHODOLOGY:Biofilm biomass quantification assay was performed to evaluate the...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Kannappan A,Sivaranjani M,Srinivasan R,Rathna J,Pandian SK,Ravi AV

    更新日期:2017-10-01 00:00:00

  • Studies on early association of Salmonella typhimurium with intestinal mucosa in vivo and in vitro: relationship to virulence.

    abstract::The abilities of six strains of Salmonella typhimurium to associate with rabbit ileal mucosa have been measured in vitro. Two were "virulent" strains (TML and W118 which are invasive and inducers of fluid secretion in rabbit ileal loops); four were "avirulent" (LT7, M206 and SL1027 which are invasive but induce neglig...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Worton KJ,Candy DC,Wallis TS,Clarke GJ,Osborne MP,Haddon SJ,Stephen J

    更新日期:1989-08-01 00:00:00

  • Impedance monitoring of bacterial activity.

    abstract::Bacterial activity and growth were monitored by following the changes of electrical impedance of cultures in liquid media. The signal is expressed automatically as a curve similar to grwoth curves produced by other methods. The technique offers a new, rapid and sensitive means of detecting active micro-organisms and i...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Ur A,Brown DF

    更新日期:1975-02-01 00:00:00

  • An increase in p62/NBR1 levels in melioidosis patients of Sri Lanka exhibit a characteristic of potential host biomarker.

    abstract::Introduction. Melioidosis, caused by Burkholderia pseudomallei, in endemic areas, poses a challenge for treating the diseased populations without accurate diagnosis, and the disease-specific biomarkers linked with the infection have yet to be reported. Due to the invasive nature of the causative agent, Burkholderia ps...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Saikh KU,Ranji CM,Ulrich RG,Corea E,De Silva AD,Natesan M

    更新日期:2020-10-01 00:00:00

  • The contribution of a capsule to survival of staphylococci within bovine neutrophils.

    abstract::The encapsulated strain M of Staphylococcus aureus and the encapsulated strain 76 of S. simulans survived incubation with bovine neutrophils in media containing heated bovine serum 0.5% v/v or milk whey 10% v/v. With two unencapsulated strains of S. aureus, M12 and M60, less than 5% of the original inoculum survived. ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Anderson JC,Williams MR

    更新日期:1985-12-01 00:00:00

  • Heterogeneity within apparently pure cultures of Escherichia coli freshly isolated from significant bacteriuria.

    abstract::Pyrolysis mass spectrometry (PyMS), a highly discriminatory method for comparison of isolates, was used to assess the homogeneity of colonies taken from apparently pure cultures of Escherichia coli in 10 urine specimens. Ten randomly selected colonies were subcultured from each urine for comparison. For six urines, th...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Freeman R,Sisson PR,Burdess D

    更新日期:1996-11-01 00:00:00

  • Molecular analysis and species diversity of Nocardia in the hospital environment in a developing country, a potential health hazard.

    abstract:PURPOSE:Despite hundreds of reports on the isolation of Nocardia from clinical samples, the presence and diversity of Nocardia species that are capable of survival in a harsh and adverse condition, such as a hospital environment, have not been comprehensively studied. The aim of this study was to assess Nocardia specie...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Rahdar HA,Azadi D,Shojaei H,Daei-Naser A

    更新日期:2017-03-01 00:00:00

  • Survival of bactericidal antibiotic treatment by tolerant persister cells of Klebsiella pneumoniae.

    abstract:PURPOSE:Persister cells, a subpopulation of tolerant cells within the bacterial culture, are commonly thought to be responsible for antibiotic therapy failure and infection recurrence. Klebsiella pneumoniae is a notorious human pathogen for its increasing resistance to antibiotics and wide involvement in severe infecti...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Li Y,Zhang L,Zhou Y,Zhang Z,Zhang X

    更新日期:2018-03-01 00:00:00

  • Antibiotics involved in Clostridium difficile-associated disease increase colonization factor gene expression.

    abstract::Clostridium difficile is the most common cause of antibiotic-associated diarrhoea. Antibiotics are presumed to disturb the normal intestinal microbiota, leading to depletion of the barrier effect and colonization by pathogenic bacteria. This first step of infection includes adherence to epithelial cells. We investigat...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Denève C,Deloménie C,Barc MC,Collignon A,Janoir C

    更新日期:2008-06-01 00:00:00