Bacterial growth and toxin production in ileostomy effluents.


:Escherichia coli (2), Vibrio cholerae (2) and Aeromonas sobria (1) strains were examined for their ability to grow and produce toxins in samples of ileostomy fluid. Three categories of response were observed: no detectable growth, growth without detectable toxin, and growth with detectable toxin. Clear differences were apparent between samples of ileostomy fluid obtained from different individuals and between samples obtained from the same individual at different times. The patterns of response were unique for each of the five test strains. We propose that the procedure developed forms a basis for investigating the host-parasite relationship in diarrhoeal disease.


J Med Microbiol


Ala Aldeen DA,Barer MR




Has Abstract


1989-04-01 00:00:00












  • Analysis of methods commonly used for glycopeptide and oxazolidinone susceptibility testing in Enterococcus faecium isolates.

    abstract::The susceptibility to teicoplanin, vancomycin and linezolid of 30 clinical isolates of Enterococcus faecium was tested by Vitek 2, Phoenix, Etest, broth microdilution and disc diffusion tests. The vanA and vanB resistance genes and the 23S rRNA gene G2576T mutation were detected by PCR and PCR-RFLP, respectively. Resi...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Raponi G,Ghezzi MC,Gherardi G,Lorino G,Dicuonzo G

    更新日期:2010-06-01 00:00:00

  • Development and application of two independent real-time PCR assays to detect clinically relevant Mucorales species.

    abstract::PCR-based detection of Mucorales species could improve diagnosis of suspected invasive fungal infection, leading to a better patient outcome. This study describes two independent probe-based real-time PCR tests for detection of clinically relevant Mucorales, targeting specific fragments of the 18S and the 28S rRNA gen...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Springer J,Goldenberger D,Schmidt F,Weisser M,Wehrle-Wieland E,Einsele H,Frei R,Löffler J

    更新日期:2016-03-01 00:00:00

  • Molecular characterization of enterovirus 71 and coxsackievirus A16 using the 5' untranslated region and VP1 region.

    abstract::Enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) are members of the species Human enterovirus A, and are both major and independent aetiological agents of hand-foot-and-mouth disease. The human enterovirus (HEV) 5' untranslated region (UTR) is fundamentally important for efficient virus replication and for virulen...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Zhou F,Kong F,Wang B,McPhie K,Gilbert GL,Dwyer DE

    更新日期:2011-03-01 00:00:00

  • The purification, structure and synthesis of proticine 3.

    abstract::The ability of Proteus mirabilis to produce the bacteriocin, proticine 3, is found almost exclusively in strains that cause severe infections of the upper urinary tract. Proticine 3 was purified from lysates of mitomycin C-induced cultures. Biological activity was associated with structures resembling bacteriophage ta...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Senior BW

    更新日期:1983-08-01 00:00:00

  • Prevalence and virulence characteristics of enteroaggregative Escherichia coli in a case-control study among patients from Iran.

    abstract::Enteroaggregative Escherichia coli (EAEC) is an important agent of diarrhoeal diseases worldwide. The role of EAEC virulence factors in the clinical outcome of infection is not completely defined. This case-control study investigated the prevalence of EAEC, its virulence genes and the antimicrobial resistance profile ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Bafandeh S,Haghi F,Zeighami H

    更新日期:2015-05-01 00:00:00

  • A collaborative evaluation of a rapid, semi-automated identification system for gram-negative bacilli: the Quantum II BID.

    abstract::The recently-introduced semi-automated bacterial identification system, the Quantum II BID, is designed to identify fermentative and non-fermentative gram-negative bacilli in 4-5 h. The system was evaluated independently by the two participating laboratories. Inter-laboratory reproducibility was determined by testing ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Stevens M,Henrichsen C,Smith M

    更新日期:1986-03-01 00:00:00

  • Effect of sugars and metabolic intermediates on the attachment of Treponema pallidum to rabbit cells.

    abstract::The effect of various energy sources and metabolic intermediates on the attachment of Treponema pallidum to baby rabbit genital organ (BRGO) cells in culture was examined. Pyruvate and glucose enhanced the motility of T. pallidum in vitro. Pyruvate increased significantly the attachment of treponemes to BRGO cells whe...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Wong GH,Steiner BM

    更新日期:1984-04-01 00:00:00

  • Identification and characterization of transmissible Pseudomonas aeruginosa strains in cystic fibrosis patients in England and Wales.

    abstract::Most past studies of cross-infection with Pseudomonas aeruginosa among cystic fibrosis (CF) patients in the UK suggest that it is a rare occurrence. However, two recent reports of highly transmissible strains in patients in regional centres in England (Liverpool and Manchester) have raised questions as to the extent o...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Scott FW,Pitt TL

    更新日期:2004-07-01 00:00:00

  • Identification, quantification and subtyping of Gardnerella vaginalis in noncultured clinical vaginal samples by quantitative PCR.

    abstract::Gardnerella vaginalis is an important component of the human vaginal microflora. It is proposed to play a key role in the pathogenesis of bacterial vaginosis (BV), the most common vaginal condition. Here we describe the development, validation and comparative analysis of a novel molecular approach capable of G. vagina...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Balashov SV,Mordechai E,Adelson ME,Gygax SE

    更新日期:2014-02-01 00:00:00

  • Incidence of common pyocin types of Pseudomonas aeruginosa from patients with cystic fibrosis and chronic airways diseases.

    abstract::We sought evidence to determine if particular strains of Pseudomonas aeruginosa have a predilection for pulmonary colonisation in patients with cystic fibrosis (CF). The incidence of common pyocin types in non-CF isolates (74%) was similar to that noted in previous reports but differed significantly (X2 = 16.7, p less...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Tredgett MW,Doherty C,Govan JR

    更新日期:1990-07-01 00:00:00

  • Detection of ISAba1 in association with a novel allelic variant of the β-lactamase ADC-82 and class D β-lactamase genes mediating carbapenem resistance among the clinical isolates of MDR A. baumannii.

    abstract:PURPOSE:The objective of the present study is to investigate the diverse resistance determinants, their association with insertion sequence mobile elements and predilection of a particular clone for such associations in Acinetobacter baumannii. METHODOLOGY:Fifty-four consecutive isolates collected during 2011-2012 fro...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Saranathan R,Kumari R,Kalaivani R,Suresh S,Rani A,Purty S,Prashanth K

    更新日期:2017-03-01 00:00:00

  • Detection of species-specific and cross-reactive cell-surface antigens of Bacteroides species by an indirect enzyme-linked immunosorbent assay.

    abstract::EDTA-released outer-membrane antigen complexes were prepared from 172 laboratory and reference strains and 43 fresh clinical or faecal isolates representing 20 species or subspecies of Bacteroides, together with 13 species of other genera. These antigens were titrated against antisera to whole, live cells of 21 specie...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Poxton IR,Brown R,Collee JG

    更新日期:1982-05-01 00:00:00

  • Identification of Burkholderia cepacia complex pathogens by rapid-cycle PCR with fluorescent hybridization probes.

    abstract::Members of the Burkholderia cepacia complex are important bacterial pathogens in cystic fibrosis (CF) patients. The B. cepacia complex currently consists of nine genetic subgroups (genomovars) of different epidemiological relevance and possibly of different pathogenic potential in humans. In this study, a new approach...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Vonberg RP,Häußler S,Vandamme P,Steinmetz I

    更新日期:2006-06-01 00:00:00

  • Evaluation of the Speed-Oligo Mycobacteria assay for the identification of nontuberculous mycobacteria.

    abstract::Nontuberculous mycobacteria (NTM) causing human infectious disease have become increasingly common. Rapid and accurate identification to the species level is, therefore, critical. The Speed-Oligo Mycobacteria assay is an oligochromatographic method that was made available recently for the identification and differenti...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Ramis IB,Cnockaert M,Von Groll A,Mathys V,Simon A,Tortoli E,Palomino JC,Almeida da Silva PE,Vandamme P,Andre E,Martin A

    更新日期:2015-03-01 00:00:00

  • The effect of antibiotics that inhibit cell-wall, protein, and DNA synthesis on the growth and morphology of Legionella pneumophila.

    abstract::The response of Legionella pneumophila to antibiotics that inhibit cell-wall, protein and DNA synthesis was examined by electronmicroscopy, MIC estimations and viable counts. Ampicillin, cefotaxime, methicillin, erythromycin, rifampicin and ciprofloxacin, each used separately at 20 times their respective MIC values, s...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Rodgers FG,Tzianabos AO,Elliott TS

    更新日期:1990-01-01 00:00:00

  • Human herpesvirus 6: molecular biology and clinical features.

    abstract::Human herpesvirus 6 (HHV-6) exists as distinct variants HHV-6A and HHV-6B. The complete genomes of HHV-6A and HHV-6B have been sequenced. HHV-6B contains 97 unique genes. CD46 is the cell receptor for HHV-6, explaining its broad tissue tropism but its restricted host-species range. HHV-6 utilizes a number of strategie...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章,评审


    authors: Dockrell DH

    更新日期:2003-01-01 00:00:00

  • Genomic profile of Brazilian methicillin-resistant Staphylococcus aureus resembles clones dispersed worldwide.

    abstract:PURPOSE:Comparative genomic analysis of strains may help us to better understand the wide diversity of their genetic profiles. The aim of this study was to analyse the genomic features of the resistome and virulome of Brazilian first methicillin-resistant Staphylococcus aureus (MRSA) isolates and their relationship to ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: de Carvalho SP,de Almeida JB,de Freitas LM,Guimarães AMS,do Nascimento NC,Dos Santos AP,Campos GB,Messick JB,Timenetsky J,Marques LM

    更新日期:2019-05-01 00:00:00

  • Continuous culture of sessile human oropharyngeal microbiotas.

    abstract::The microbiota of the human oropharynx plays an important role in health through involvement in the aetiology of infection and the carriage of adventitious pathogens. Despite this, there are few models available for the preclinical assessment of novel antimicrobials directed to the human throat. We have profiled bacte...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Humphreys GJ,McBain AJ

    更新日期:2013-06-01 00:00:00

  • Leclercia adecarboxylata in an immunocompetent patient.

    abstract::Leclercia adecarboxylata is a rarely reported human pathogen, most commonly affecting immunocompromised individuals. In reported cases of immunocompetent patients infected with this organism, it is seen exclusively in the context of polymicrobial infections. We report here the case of an abscess in an immunocompetent ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Hess B,Burchett A,Huntington MK

    更新日期:2008-07-01 00:00:00

  • Evidence for systemic spread of the potentially zoonotic intestinal spirochaete Brachyspira pilosicoli in experimentally challenged laying chickens.

    abstract::Brachyspira pilosicoli is a potentially zoonotic anaerobic intestinal spirochaete that is one of several species causing avian intestinal spirochaetosis. The aim of this study was to develop a reproducible model of infection in point-of-lay chickens and compare the virulence of two strains of B. pilosicoli in a model ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Mappley LJ,Tchórzewska MA,Nunez A,Woodward MJ,La Ragione RM

    更新日期:2013-02-01 00:00:00

  • Inhibition of cell proliferation and induction of apoptosis by Helicobacter pylori through increased phosphorylated p53, p21 and Bax expression in endothelial cells.

    abstract::Microcirculation plays a crucial role in mucosal physiological function as well as repair of gastric mucosal damage. Endothelial cell damage is known to disturb microcirculation and suppress angiogenesis. Therefore, the direct effect of Helicobacter pylori on endothelial cells in vitro was investigated with H. pylori ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Kurosawa A,Miwa H,Hirose M,Tsune I,Nagahara A,Sato N

    更新日期:2002-05-01 00:00:00

  • Lior-serotype variants in Campylobacter isolates from the same stool sample.

    abstract::Campylobacter strains isolated from the same stool sample were characterised by determination of biochemical properties and both heat-labile (Lior) and heat-stable (Lauwers) serotypes. In six of 60 campylobacter-infected stools, two or three strains differing in Lior-serotype were isolated from the same stool. In four...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Zöllner B,Wuthe HH

    更新日期:1993-01-01 00:00:00

  • Experimental infection of germ-free mice with hyper-toxigenic enterohaemorrhagic Escherichia coli O157:H7, strain 6.

    abstract::A mouse enterohaemorrhagic Escherichia coli (EHEC) infection model was developed with a combination of germ-free (GF) mice and hyper-toxigenic EHEC (HT-EHEC) O157:H7 strain 6. The HT-EHEC strain 6 produced both Shiga-like toxin (SLT)-1 1.0 microg/mI and SLT-2 8.2 microg/ml in vitro. Eight-week-old germ-free mice were ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Taguchi H,Takahashi M,Yamaguchi H,Osaki T,Komatsu A,Fujioka Y,Kamiya S

    更新日期:2002-04-01 00:00:00

  • RNA-dependent DNA-polymerase activity in human milk.

    abstract::A simple method is described for testing milk specimens from nursing mothers for the presence of RNA-dependent DNA-polymerase activity. Positive results were obtained in five of 137 women (3.6%) without a family history of breast cancer, and in six of 31 women (19.3%) with a family history of breast cancer. ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: McFarlane ES,Ryan SM,Mann E

    更新日期:1975-08-01 00:00:00

  • Genetic diversity of Propionibacterium acnes strains isolated from human skin in Japan and comparison with their distribution in Europe.

    abstract::Propionibacterium acnes, a commensal of human skin, is also an opportunistic pathogen of common acne and certain infectious diseases. However, it is still not obvious which strain is pathogenic for a certain infectious disease, and investigations to characterize pathogenic strains using molecular typing methods such a...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Dekio I,Rajendram D,Morita E,Gharbia S,Shah HN

    更新日期:2012-05-01 00:00:00

  • Demonstration of spirochaetes in patients with Lyme disease with a modified silver stain.

    abstract::Spirochaetes were demonstrated in material from patients with Lyme disease by short-time high-concentrate silver impregnation after treatment with amylase. Removal of mucoid material was essential to visualise Borrelia burgdorferi. Lyme spirochaetes were demonstrated in material from 23 patients with Lyme disease--ery...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: De Koning J,Bosma RB,Hoogkamp-Korstanje JA

    更新日期:1987-05-01 00:00:00

  • Maternofetal infections due to Eikenella corrodens.

    abstract::Eikenella corrodens, a commensal of the human oral cavity, is generally associated with bite wounds and head and neck infections. Neonatal infections are rare. We report two cases of premature birth associated with maternofetal E. corrodens infection. ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Garnier F,Masson G,Bedu A,Masson P,Decroisette E,Guigonis V,Fermeaux V,De Barbentane MC,Denis F,Ploy MC

    更新日期:2009-02-01 00:00:00

  • Haemolysin produced by Vibrio cholerae non-O1 is not enterotoxic.

    abstract::Of 28 isolates of Vibrio cholerae non-O1 (10 from diarrhoeal patients and 18 from environmental sources) examined for haemolytic activity and its correlation, if any, with enterotoxic activity, 24 showed haemolysis. The four non-haemolytic isolates showed haemolysis after consecutive passages through rabbit ileal loop...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Singh DV,Shukla BN,Sanyal SC

    更新日期:1996-07-01 00:00:00

  • Severe case of Mediterranean spotted fever in Greece with predominantly neurological features.

    abstract::Rickettsia conorii is endemic in Greece, though only a few cases of infection have been published to date. The case of a 58-year-old man from northern Greece with a severe form of Mediterranean spotted fever and rapid neurological deterioration is presented here. The patient received antibiotic treatment with doxycycl...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Tzavella K,Hatzizisis IS,Vakali A,Mandraveli K,Zioutas D,Alexiou-Daniel S

    更新日期:2006-03-01 00:00:00

  • Visceral leishmaniasis/human immunodeficiency virus co-infection in India: the focus of two epidemics.

    abstract::India contributes heavily to the global burden of visceral leishmaniasis (VL, kala-azar) and human immunodeficiency virus (HIV)/AIDS. The prevalence of HIV seropositivity in VL patients at a tertiary care centre in northern India, as observed during a prospective study over a period of 2 years, is presented. Of the 10...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Mathur P,Samantaray JC,Vajpayee M,Samanta P

    更新日期:2006-07-01 00:00:00