Genetic and phenotypic characterization of Candida albicans strains isolated from infectious disease patients in Shanghai.

abstract：:Providencia alcalifaciens is an invasive enteric pathogen. The present study determined the prevalence of invasive ability in P. alcalifaciens strains isolated in São Paulo, Brazil, mainly from patients with diarrhoea. Invasion of HeLa cells was found in 17 (42%) of 41 strains studied. Most (88%) of the invasive strai...

journal_title：Journal of medical microbiology

authors： Guth BE,Perrella E

更新日期：1996-12-01 00:00:00

abstract：:The prevalence and genotype of Shiga-like toxins (SLTs) in Escherichia coli (O)157 strains from patients in Germany with haemolytic uraemic syndrome (HUS) were investigated. This was done by PCR amplification of the B-subunit genes with two primer pairs--one complementary to slt-IB, and the other homologous to both sl...

journal_title：Journal of medical microbiology

authors： Rüssmann H,Schmidt H,Heesemann J,Caprioli A,Karch H

更新日期：1994-05-01 00:00:00

abstract：:Fusarium spp. and other non-dermatophyte fungi are repeatedly isolated from abnormal nails. To investigate whether these fungi are the aetiological agents of infection or simply transient contaminants, a PCR/sequencing/RFLP assay was developed for direct and routine identification of the infecting fungi in onychomycos...

journal_title：Journal of medical microbiology

authors： Monod M,Bontems O,Zaugg C,Léchenne B,Fratti M,Panizzon R

更新日期：2006-09-01 00:00:00

abstract：:Introduction. Differences between the genomic and virulence profile of Bordetella pertussis circulating strains and vaccine strains are considered as one of the important reasons for the resurgence of whooping cough (pertussis) in the world. Genetically inactivated B. pertussis is one of the new strategies to generate...

journal_title：Journal of medical microbiology

authors： Nikbin VS,Keramati M,Noofeli M,Tayebzadeh F,Kahali B,Shahcheraghi F

更新日期：2020-01-01 00:00:00

abstract：:Fifty clinical strains of Chlamydia trachomatis were studied by denaturing gradient gel electrophoresis (DGGE) of bacterial DNA amplified by the polymerase chain reaction (PCR). The strains belonged to the three most commonly encountered serovars in developed countries--D, E and F. Six reference strains, including the...

journal_title：Journal of medical microbiology

authors： Sayada C,Denamur E,Grandchamp B,Orfila J,Elion J

更新日期：1995-07-01 00:00:00

abstract：:Administration of oestradiol to three strains of female mice induced the oestrous phase of the reproductive cycle in which there are few or no polymorphonuclear leucocytes (PMNL) in vaginal smears. This treatment rendered the mice susceptible to genital tract colonisation by serotype 8 of Ureaplasma urealyticum, inocu...

journal_title：Journal of medical microbiology

authors： Furr PM,Taylor-Robinson D

更新日期：1989-06-01 00:00:00

abstract：:Small round viruses (SRV) were isolated from the faeces of diarrhoeic calves from three farms. All three SRV preparations caused diarrhoea experimentally in gnotobiotic calves. Each preparation contained viral particles of two morphological types, "astrovirus-like" and "calicivirus-like", and from one preparation the ...

journal_title：Journal of medical microbiology

authors： Woode GN,Bridger JC

更新日期：1978-11-01 00:00:00

abstract：:Fifteen randomly selected nasopharyngeal (NP) swab specimens (culture-negative for influenza A virus) were spiked with influenza A virus and the nucleic acids were extracted and subjected to PCR amplification with Thermus aquaticus (Taq) and T. thermophilus (Tth) DNA polymerases. Products of the expected size, and giv...

journal_title：Journal of medical microbiology

authors： Poddar SK,Sawyer MH,Connor JD

更新日期：1998-12-01 00:00:00

abstract：:Yeast identification and in vitro susceptibility testing provide helpful information for appropriate administration of antifungal treatments; however, few reports from the Latin American region have been published. The aim of this study was to identify the species present in isolates from bloodstream infections diagno...

journal_title：Journal of medical microbiology

authors： Bustamante B,Martins MA,Bonfietti LX,Szeszs MW,Jacobs J,Garcia C,Melhem MSC

更新日期：2014-06-01 00:00:00

abstract：:Campylobacter-like organisms, isolated from the gastric antrum of Rhesus monkeys, were compared with Campylobacter jejuni and C. pylori. They were similar to C. pylori by light microscopy, in ultrastructural morphology, in enzymic, fatty-acid-methyl-ester, and protein-profile analysis, and in antigenic reactivity with...

journal_title：Journal of medical microbiology

authors： Newell DG,Hudson MJ,Baskerville A

更新日期：1988-09-01 00:00:00

abstract：:A total of 69 strains of Escherichia coli from patients in the Taizhou Municipal Hospital, China, were isolated, and 11 strains were identified that were resistant to bacitracin, chloramphenicol, tetracycline and erythromycin. These strains were PCR positive for at least two out of three genes, ybjG, dacC and mdfA, by...

journal_title：Journal of medical microbiology

authors： Wang D,Hu E,Chen J,Tao X,Gutierrez K,Qi Y

更新日期：2013-11-01 00:00:00

abstract：:A non-tuberculous mycobacterium was isolated, following a vertebral needle aspiration, from the blood of a patient with severe spondylodiscitis. The strain turned out to be different from any known mycobacterial species and was quite drug-susceptible in vitro. The patient improved markedly following treatment with mer...

journal_title：Journal of medical microbiology

authors： Tortoli E,Mantella A,Mariottini A,Mazzarelli G,Pecile P,Rogasi PG,Sterrantino G,Fantoni E,Leoncini F

更新日期：2006-01-01 00:00:00

abstract：:The toxic effects of the haemorrhagic toxin of Clostridium sporogenes were studied in mice, rats, guinea-pigs and rabbits, and in various cultured cells. In rabbits, but not in the other animals, intradermal injection with crude toxin and its injection into a ligated intestinal loop caused haemorrhage in both the skin...

journal_title：Journal of medical microbiology

authors： Hara-Kudo Y,Ogura A,Noguchi Y,Kumagai S

更新日期：1997-04-01 00:00:00

abstract：:We have characterised 45 isolates of methicillin-resistant Staphylococcus aureus (MRSA) from Glasgow Royal Infirmary by means of simple biotyping, immunoblotting of exported proteins and restriction enzyme fragmentation patterns (REFP) of plasmid DNA. The strains were subdivided into four groups (A-D) on the basis of ...

journal_title：Journal of medical microbiology

authors： Coia JE,Thomson-Carter F,Baird D,Platt DJ

更新日期：1990-02-01 00:00:00

abstract：:A total of 63 Aeromonas strains isolated from diarrhoeal faeces or water samples were tested for adhesion to HEp-2 cells. An association between diarrhoea and high level adhesion was observed in that 12 of the 34 faecal isolates and none of the 29 environmental isolates yielded greater than 20 bacteria per HEp-2 cell ...

journal_title：Journal of medical microbiology

authors： Carrello A,Silburn KA,Budden JR,Chang BJ

更新日期：1988-05-01 00:00:00

abstract：:Chickens were immunised with a preparation of purified 14-kDa fimbriae of Salmonella serotype Enteritidis (SEF 14) to raise egg-yolk antibodies for protection trials in mice against subsequent challenge-exposure with the homologous strain of Enteritidis. A pronounced specificity of egg-yolk antibodies against the 14-k...

journal_title：Journal of medical microbiology

authors： Peralta RC,Yokoyama H,Ikemori Y,Kuroki M,Kodama Y

更新日期：1994-07-01 00:00:00

abstract：:Recent studies have shown that Providencia alcalifaciens is a diarrhoeal pathogen. It may cause diarrhoea by an invasive mechanism, as it invades cultured mammalian cells in vitro and intestinal epithelial cells of experimentally inoculated rabbits in vivo. To locate the gene(s) involved in invasion, TnphoA mutants of...

journal_title：Journal of medical microbiology

authors： Rahman M,Monira S,Nahar S,Ansaruzzaman M,Alam K,Alam M,Albert MJ

更新日期：2002-08-01 00:00:00

abstract：:Since 2007, there has been a re-emergence of cholera outbreaks in northern Vietnam. To understand the molecular epidemiological relatedness and determine the antibiotic susceptibility profiles of responsible V. cholerae O1 outbreak strains, a representative collection of 100 V. cholerae O1 strains was characterized. V...

journal_title：Journal of medical microbiology

authors： Tran HD,Alam M,Trung NV,Van Kinh N,Nguyen HH,Pham VC,Ansaruzzaman M,Rashed SM,Bhuiyan NA,Dao TT,Endtz HP,Wertheim HFL

更新日期：2012-03-01 00:00:00

abstract：:The rat was evaluated as an experimental model for disseminated candidosis by quantitating blood clearance and initial organ localisation of 3H-leucine-labelled Candida albicans after intravenous injection into the tail or portal vein. Viable or formalin-killed blastoconidia or viable blastoconidia with germ tubes wer...

journal_title：Journal of medical microbiology

authors： Katz S,Grosfeld JL,Folkening WJ,Rosenthal RS,Merkel GJ

更新日期：1992-10-01 00:00:00

abstract：:The ability of cefotaxime, ciprofloxacin, piperacillin and tobramycin to cause release of endotoxin was examined in vitro with cultures of Enterobacter cloacae and Escherichia coli. Endotoxin was measured by a quantitative limulus amoebocyte lysate assay and its presence was confirmed by silver staining of the lipopol...

journal_title：Journal of medical microbiology

authors： Crosby HA,Bion JF,Penn CW,Elliott TS

更新日期：1994-01-01 00:00:00

abstract：:The care of many patients undergoing long-term bladder catheterization is frequently complicated by infection with Proteus mirabilis. These organisms colonize the catheter, forming surface biofilm communities, and their urease activity generates alkaline conditions under which crystals of magnesium ammonium phosphate ...

journal_title：Journal of medical microbiology

authors： Jones BV,Mahenthiralingam E,Sabbuba NA,Stickler DJ

更新日期：2005-09-01 00:00:00

abstract：:Although Neisseria gonorrhoeae can use haem as the sole exogenous iron source for growth in vitro, the mechanism of haem-iron uptake in the gonococcus is unknown. Two haemin-binding proteins (HmBPs) of 97 and 44 Kda were isolated by batch ligand affinity-chromatography from whole cells or total membranes of gonococci ...

journal_title：Journal of medical microbiology

authors： Lee BC

更新日期：1992-02-01 00:00:00

abstract：:Subtractive hybridisation was used to screen for and identify conserved DNA sequences associated with clinical, clonal populations of Staphylococcus aureus. DNA from S. aureus strain ISP8 (a clinical isolate) was digested with TaqI and hybridised with randomly fragmented pooled DNA from 10 non-clinical (community) iso...

journal_title：Journal of medical microbiology

authors： el-Adhami WA,Stewart PR,Matthaei KI

更新日期：1997-12-01 00:00:00

abstract：:Borderline oxacillin-resistant Staphylococcus aureus (BORSA) represents a quite poorly understood and inadequately defined phenotype of methicillin resistance. BORSA strains show low, borderline resistance to penicillinase-resistant penicillins (PRPs), with oxacillin MICs typically equal to 1-8 µg ml-1, and in contras...

journal_title：Journal of medical microbiology

authors： Hryniewicz MM,Garbacz K

更新日期：2017-10-01 00:00:00

abstract：:A colony hybridisation method with enzyme-labelled oligonucleotide probes was developed to detect the gene for toxic shock syndrome toxin 1 (tst). For rapid identification, bacterial colonies were transferred from agar plates directly on to nylon membranes. These procedures took only 3 h. Results obtained by this test...

journal_title：Journal of medical microbiology

authors： Shimaoka M,Yoh M,Takarada Y,Yamamoto K,Honda T

更新日期：1996-03-01 00:00:00

abstract：:Clostridium difficile is the most common cause of nosocomial bacterial diarrhoea in the Western world. Diarrhoea and colitis are caused by the actions of toxins A and B released by pathogenic strains of C. difficile. Adaptive immune responses to these toxins influence the outcomes of C. difficile infection (CDI). Symp...

journal_title：Journal of medical microbiology

authors： Kelly CP,Kyne L

更新日期：2011-08-01 00:00:00

abstract：:Microcirculation plays a crucial role in mucosal physiological function as well as repair of gastric mucosal damage. Endothelial cell damage is known to disturb microcirculation and suppress angiogenesis. Therefore, the direct effect of Helicobacter pylori on endothelial cells in vitro was investigated with H. pylori ...

journal_title：Journal of medical microbiology

authors： Kurosawa A,Miwa H,Hirose M,Tsune I,Nagahara A,Sato N

更新日期：2002-05-01 00:00:00

abstract：:A total of 817 human clinical isolates of Clostridium difficile from all Australian states were screened for A(-)B(+) strains by toxin gene PCR assays. Nine (1.1 %) strains were confirmed to be A(-)B(+) by enzyme immunoassay for toxin production. Of these, six (66.7 %) were binary toxin-positive by PCR. Using PCR ribo...

journal_title：Journal of medical microbiology

authors： Elliott B,Squire MM,Thean S,Chang BJ,Brazier JS,Rupnik M,Riley TV

更新日期：2011-08-01 00:00:00

abstract：:Multilocus sequence typing (MLST) is considered a reliable method for providing insight into the Streptococcus suis population structure, clonal relationships and the potential of particular clones to cause disease. Indeed, MLST has revealed the presence of several clonal complexes (CCs) within the Streptococcus suis ...

journal_title：Journal of medical microbiology

authors： Hatrongjit R,Kerdsin A,Gottschalk M,Hamada S,Oishi K,Akeda Y

更新日期：2016-05-01 00:00:00

abstract：:Antigen participating in cell-mediated immunity was isolated from a sonic extract of Mycobacterium tuberculosis H37Rv. The purified antigen had a mol. wt 150 000-200 000, based upon its Rf in 7.5% polyacrylamide gel in the presence of sodium dodecyl sulphate. In simple agarose gel electrophoresis it had an Rf of 0.85....

journal_title：Journal of medical microbiology

authors： Hattikudur S,Kamat RS

更新日期：1984-08-01 00:00:00