Detection of the gene for toxic shock syndrome toxin 1 in Staphylococcus aureus by enzyme-labelled oligonucleotide probes.

abstract：:Five strains of mice (C3H, CBA, BALB/c, TO and ICR) were inoculated intra-vaginally with Neisseria gonorrhoeae in an attempt to produce an animal model of gonorrhoea. Of a total of 68 mice inoculated, only three (4.4%) were culture-positive after 3 days. Histological examination of both the genital mucosa of inoculate...

journal_title：Journal of medical microbiology

authors： Johnson AP,Tuffrey M,Taylor-Robinson D

更新日期：1989-09-01 00:00:00

abstract：INTRODUCTION:Idiopathic pulmonary fibrosis (IPF) is a chronic and ultimately fatal lung disease. One of the risk factors involved in the acquisition of IPF is viral infections, especially respiratory viruses. In the present study, we investigated the detection of respiratory viruses and the possible relationship betwee...

journal_title：Journal of medical microbiology

authors： Keyvani H,Moghoofei M,Bokharaei-Salim F,Mostafaei S,Javad Mousavi SA,Monavari SH,Esghaei M

更新日期：2017-11-01 00:00:00

abstract：:Two chlamydial enzyme immunoassays, Chlamydiazyme and IDEIA, were evaluated in a mouse model of chlamydial genital-tract infection. The Chlamydiazyme assay and the IDEIA were assessed on specimens from 10 and 11 mice, respectively. The animals were infected with Chlamydia trachomatis, strain SA2f, and the results obta...

journal_title：Journal of medical microbiology

authors： Taylor-Robinson D,Tuffrey M

更新日期：1987-09-01 00:00:00

abstract：:The ability of Streptococcus mutans to produce mutacins, combined with the production of other virulence factors such as lactic acid, may contribute to the pathogenesis of this bacterium. In the present study, the detection of genes encoding mutacin types I/III, II and IV was performed by PCR with specific primers to ...

journal_title：Journal of medical microbiology

authors： Kamiya RU,Napimoga MH,Höfling JF,Gonçalves RB

更新日期：2005-06-01 00:00:00

abstract：:Salmonella enterica serovar Typhimurium (S. Typhimurium) is the commonest pathogen causing food-borne disease among humans and animals in Australia. A multiplex PCR-based reverse line blot (mPCR/RLB) system was developed to rapidly identify S. Typhimurium phage types and strains within them. The system comprised 32 bi...

journal_title：Journal of medical microbiology

authors： Wang Q,Kong F,Jelfs P,Gilbert GL

更新日期：2008-07-01 00:00:00

abstract：:The prevalence of Shiga toxin-producing Escherichia coli (STEC) and its characteristics were determined among hospitalized patients with diarrhoea and children with diarrhoea in an urban slum community of Dhaka city using sensitive culture and PCR methods. Stool samples were collected from 410 patients with diarrhoea ...

journal_title：Journal of medical microbiology

authors： Islam MA,Heuvelink AE,de Boer E,Sturm PD,Beumer RR,Zwietering MH,Faruque ASG,Haque R,Sack DA,Talukder KA

更新日期：2007-03-01 00:00:00

abstract：:Five hundred clinical group A streptococcal (GAS) isolates were collected in Belgium during the period 1 Nov. 1993 to 31 Oct. 1994. Clinical and laboratory data were recorded and isolates were characterised. The presence of the genes encoding streptococcal pyrogenic exotoxin types A (speA), B (speB), C (speC), F (speF...

journal_title：Journal of medical microbiology

authors： Descheemaeker P,VAN Loock F,Hauchecorne M,Vandamme P,Goossens H

更新日期：2000-05-01 00:00:00

abstract：:To gain insight into the value of lipopolysaccharide (LPS) core determinants for cross-protective immunisation the serological relationships between six complete (LPS) core types from Enterobacteriaceae were investigated. Hyperimmune sera were raised in mice by repeated immunisation with heat-killed strains of Salmone...

journal_title：Journal of medical microbiology

authors： Nnalue NA,Khan GN,Mustafa N

更新日期：1999-05-01 00:00:00

abstract：:This study investigated the comparative performance of the Amplicor assay and an in-house semi-automated, multiplex real-time PCR for the diagnosis of genital chlamydial infection. Four different assays, the COBAS Amplicor CT test (Amplicor PCR), in-house real-time PCR (IHRT-PCR), in-house nested cryptic plasmid PCR a...

journal_title：Journal of medical microbiology

authors： Jalal H,Al-Suwaine A,Stephen H,Carne C,Sonnex C

更新日期：2007-03-01 00:00:00

abstract：:Administration of oestradiol to three strains of female mice induced the oestrous phase of the reproductive cycle in which there are few or no polymorphonuclear leucocytes (PMNL) in vaginal smears. This treatment rendered the mice susceptible to genital tract colonisation by serotype 8 of Ureaplasma urealyticum, inocu...

journal_title：Journal of medical microbiology

authors： Furr PM,Taylor-Robinson D

更新日期：1989-06-01 00:00:00

abstract：:The effects of imipenem and meropenem on the transcriptional expression of resistance-related genes oprD, oprM and oprN in Pseudomonas aeruginosa were studied by quantitative real-time PCR. Four strains were examined: the type strain PT5 (PAO1), its derivatives M7 and PT149, and a clinical isolate, PaKT3. The derivati...

journal_title：Journal of medical microbiology

authors： Kolayli F,Karadenizli A,Savli H,Ergen K,Hatirnaz O,Balikci E,Budak F,Vahaboglu H

更新日期：2004-09-01 00:00:00

abstract：:A prospective study was conducted of the rapid FASTPlaque-Response test for determination of rifampicin resistance in Mycobacterium tuberculosis with and without the addition of an antimicrobial supplement containing nystatin, oxacillin and aztreonam (NOA) to control specimen-related contamination. A total of 631 smea...

journal_title：Journal of medical microbiology

authors： Mole R,Trollip A,Abrahams C,Bosman M,Albert H

更新日期：2007-10-01 00:00:00

abstract：:We studied the immunoglobulin response to individual viral polypeptides in experimental primary and secondary infection with herpes simplex virus (HSV)-1 in mice. After a single footpad inoculation with 10(5.6) pfu of HSV-1, immunoglobulin to proteins mol. wts (10(3)) 44 and 75 appeared on day 7. Antibodies to gB, gC,...

journal_title：Journal of medical microbiology

authors： McKendall RR,Pettit M,Woo W

更新日期：1988-01-01 00:00:00

abstract：:The nucleolar accumulation of Semliki Forest Virus (SFV) C protein was examined as a function of intact microtubules, intact microfilaments and accessible intermediate filaments. The cytoskeletal components do not seem to play a role in directing C protein to the nucleolus but nucleolar accumulation is energy-dependen...

journal_title：Journal of medical microbiology

authors： Jakob R

更新日期：1994-06-01 00:00:00

abstract：:Bedaquiline (BDQ) has been proven to be effective in the treatment of multidrug-resistant tuberculosis. We hypothesized that BDQ could be a potential agent to treat nontuberculous mycobacterial (NTM) infection. The objective of this study was to evaluate the in vitro activity of BDQ against rapidly growing mycobacteri...

journal_title：Journal of medical microbiology

authors： Aguilar-Ayala DA,Cnockaert M,André E,Andries K,Gonzalez-Y-Merchand JA,Vandamme P,Palomino JC,Martin A

更新日期：2017-08-01 00:00:00

abstract：:Diagnosis of human immunodeficiency virus (HIV) infection by antibody-based testing allows for some recently infected individuals to be falsely assessed as non-infected. Since such individuals often have high viral loads and are capable of transmitting HIV, it is an imperative public health need to identify these indi...

journal_title：Journal of medical microbiology

authors： Ren A,Louie B,Rauch L,Castro L,Liska S,Klausner JD,Pandori MW

更新日期：2008-10-01 00:00:00

abstract：PURPOSE:Sporothrix brasiliensis, a member of the Sporothrix schenckii complex, is a major cause of epidemic outbreaks of sporotrichosis due to its greater virulence and ability to evade the immune system. The absence of studies about this species led to this study, with the aim to evaluate the importance of Toll-like r...

journal_title：Journal of medical microbiology

authors： Rossato L,Silvana Dos Santos S,Ferreira LG,Rogério de Almeida S

更新日期：2019-01-01 00:00:00

abstract：:The characterization of integrons and their promoters in 156 antibiotic-resistant clinical isolates of the important zoonotic pathogens Salmonella enterica subsp. enterica serovars Typhimurium (S. Typhimurium) and Enteritidis (S. Enteritidis) from Norwegian hospitals were performed. Integrons were found in 64 of 66 S....

journal_title：Journal of medical microbiology

authors： Lindstedt BA,Heir E,Nygård I,Kapperud G

更新日期：2003-02-01 00:00:00

abstract：:The National Institute of Care Excellence (NICE) 2007 guidance CG54, on urinary tract infection (UTI) in children, states that clinicians should use urgent microscopy and culture as the preferred method for diagnosing UTI in the hospital setting for severe illness in children under 3 years old and from the GP setting ...

journal_title：Journal of medical microbiology

authors： McNulty CAM,Verlander NQ,Moore PCL,Larcombe J,Dudley J,Banerjee J,Jadresic L

更新日期：2015-09-01 00:00:00

abstract：:The carriage on skin lesions, on healthy skin and in the nose, of micro-organisms that produce antibiotics active against Staphylococcus aureus has been studied. About 5% of sites (10% of all patients) yielded such organisms. No evidence could be found that the presence of producer organisms in skin lesions had a prot...

journal_title：Journal of medical microbiology

authors： Noble WC,Willie JA

更新日期：1980-05-01 00:00:00

abstract：:A slide agglutination technique identifying 10 serogroups of Clostridium difficile (A,B,C,D,F,G,H,I,K and X) has been described previously. In this study, we have used the hamster to compare the ability of the 10 serogroup reference strains to colonise and produce disease. Groups of four hamsters were each given a sin...

journal_title：Journal of medical microbiology

authors： Delmée M,Avesani V

更新日期：1990-10-01 00:00:00

abstract：:The aim of the present study was to correlate molecular evidence of the presence of Helicobacter pylori in gastric biopsy samples, based on analysis of 16S rDNA, vacuolating toxin (vacA), urease A (ureA) and cagA genes, with the clinical, histological and serological findings in patients with H. pylori-associated gast...

journal_title：Journal of medical microbiology

authors： Tiveljung A,Borch K,Jonasson J,Mårdh S,Petersson F,Monstein HJ

更新日期：1998-08-01 00:00:00

abstract：:Multi-drug-resistant tuberculosis (MDR-TB) is a major public-health problem, because treatment is complicated and patients remain infectious for months or years, despite receiving the best available therapy. To gain better understanding of MDR-TB, a retrospective study was initiated to determine the level of drug resi...

journal_title：Journal of medical microbiology

authors： Dam T,Isa M,Bose M

更新日期：2005-03-01 00:00:00

abstract：:A cornyeform bacterium was isolated from a blood culture from a 24-year-old man with familial hypertrophic non-obstructive cardiomyopathy, chronic abuse of anabolic steroids and prior admission to hospital because of clinical signs of sepsis. 16S rRNA gene analysis unambiguously identified Gordonia terrae. ...

journal_title：Journal of medical microbiology

authors： Grisold AJ,Roll P,Hoenigl M,Feierl G,Vicenzi-Moser R,Marth E

更新日期：2007-12-01 00:00:00

abstract：PURPOSE:This study aimed to evaluate the effect of two types of stress, cold and nutritional, on the viability and the in vitro virulence of the foodborne pathogenic bacteria Listeria monocytogenes. METHODOLOGY:Ten diverse isolates were kept in phosphate-buffered saline (PBS) at optimal (37 °C) or at refrigeration tem...

journal_title：Journal of medical microbiology

authors： Araujo V,Neves E,Silva AC,Martins APL,Brito LC

更新日期：2018-01-01 00:00:00

abstract：:Candida parapsilosis is the second most frequent Candida species isolated from blood cultures. Since 2005, C. parapsilosis has been divided into three distinct species based on genetic traits: Candida parapsilosis, Candida metapsilosis and Candida orthopsilosis. The aim of this study was to develop a rapid real-time P...

journal_title：Journal of medical microbiology

authors： Hays C,Duhamel C,Cattoir V,Bonhomme J

更新日期：2011-04-01 00:00:00

abstract：:Here, we describe the first report of post-operative endophthalmitis due to Comamonas testosteroni in an elderly diabetic patient after complicated cataract surgery. The isolate was identified by using Mini API strips. The patient was successfully treated with intravitreal ceftazidime and oral ciprofloxacin. ...

journal_title：Journal of medical microbiology

authors： Reddy AK,Murthy SI,Jalali S,Gopinathan U

更新日期：2009-03-01 00:00:00

abstract：:Sorbarod biofilms were investigated for their suitability in establishing continuous culture biofilms for the study of bacterial vaginosis. Two important organisms in the condition, Gardnerella vaginalis and Lactobacillus acidophilus, were studied. In contrast to growth in broth culture, both organisms were maintained...

journal_title：Journal of medical microbiology

authors： Muli FW,Struthers JK

更新日期：1998-05-01 00:00:00

abstract：:The unpredictable characteristics of meningococcal disease (MD) make outbreaks complicated to monitor and consequently lead to high levels of public anxiety. Traditional molecular techniques have been utilized in order to understand better the epidemiology of MD, but some have disadvantages such as being highly specia...

journal_title：Journal of medical microbiology

authors： Diggle MA,Bell CM,Clarke SC

更新日期：2003-06-01 00:00:00

abstract：:Different aspects of the terms strain, clone and species are discussed. The term strain is commonly used to denote a pure culture - here called 'the strain in the taxonomic sense' - but does also refer to a natural concept closely related to the clone. The term clone on the other hand is used both in a general and in ...

journal_title：Journal of medical microbiology

authors： Dijkshoorn L,Ursing BM,Ursing JB

更新日期：2000-05-01 00:00:00