Improved contamination control for a rapid phage-based rifampicin resistance test for Mycobacterium tuberculosis.

abstract：:Currently, several PCR assays based on 16S rRNA and virulence-associated genes are available for detection of Legionella pneumophila. So far, no genotyping method has been published that can discriminate between serogroups and monoclonal subgroups of the most common L. pneumophila serogroup 1. Our first approach was t...

journal_title：Journal of medical microbiology

authors： Thürmer A,Helbig JH,Jacobs E,Lück PC

更新日期：2009-05-01 00:00:00

abstract：:Large-scale monitoring of resistance to 14 antimicrobial agents was performed using 103 Proteus mirabilis strains isolated from dogs in Japan. Resistant strains were analysed to identify their resistance mechanisms. Rates of resistance to chloramphenicol, streptomycin, enrofloxacin, trimethoprim/sulfamethoxazole, kana...

journal_title：Journal of medical microbiology

authors： Harada K,Niina A,Shimizu T,Mukai Y,Kuwajima K,Miyamoto T,Kataoka Y

更新日期：2014-11-01 00:00:00

abstract：:Nosocomial infections with enterococci are an increasing problem in modern medical practice due to the development of resistance to a wide range of antibiotics, including the glycopeptides vancomycin and teicoplanin. An increasing number of vancomycin-resistant enterococci (VRE) have been cultured from clinical specim...

journal_title：Journal of medical microbiology

authors： Mackenzie CR,Hucke C,MÜller D,Seidel K,Takikawa O,DÄubener W

更新日期：1999-10-01 00:00:00

abstract：PURPOSE:Group A rotavirus (RVA) is the leading cause of severe gastroenteritis in children younger than 5 years. The most common human G-types are G1-4 and G9. G12 genotype is currently emerging worldwide, becoming the sixth most prevalent RVA G-genotype. In Tunisia, an emergence of G12 RVA strains was observed. To und...

journal_title：Journal of medical microbiology

authors： Moussa A,Fredj MBH,BenHamida-Rebaï M,Fodha I,Boujaafar N,Trabelsi A

更新日期：2017-02-01 00:00:00

abstract：PURPOSE:We aimed to evaluate a PCR-based technique for the diagnosis of mucormycosis and the identification of fungi from fresh tissue specimens in patients with rhino-orbito-cerebral-mucormycosis (ROCM). METHODOLOGY:Fifty cases of ROCM were included in the study. Conventional identification was performed using micros...

journal_title：Journal of medical microbiology

authors： Zaman K,Rudramurthy SM,Das A,Panda N,Honnavar P,Kaur H,Chakrabarti A

更新日期：2017-08-01 00:00:00

abstract：:Various factors influencing the susceptibility of C3H mice to lethal infection by the phycomycete Absidia ramosa were examined. Mice 19-21 days old were exposed to graded doses of A. ramosa spores by various routes. After intravenous inoculation with doses in excess of 10(3) viable units, a variable proportion of mice...

journal_title：Journal of medical microbiology

authors： Corbel MJ,Eades SM

更新日期：1975-11-01 00:00:00

abstract：PURPOSE:We examined evidence for transmission of Pandorea apista among cystic fibrosis (CF) patients attending paediatric and adult services in one city who had previously been found to harbour related isolates by pulsed-field gel electrophoresis (PFGE). METHODOLOGY:The whole-genome sequences of 18 isolates from this ...

journal_title：Journal of medical microbiology

authors： Kenna DTD,Coward A,Perry C,Pike R,Schaefer U,Turton J,Green H,Jones AM,Bright-Thomas RJ,Burns P,Narayan O,Wilkinson S,Turton JF

更新日期：2019-07-01 00:00:00

abstract：:The genetic characteristics of Vibrio parahaemolyticus strains isolated in 2004 and 2005 in Mozambique were assessed in this study to determine whether the pandemic clone of V. parahaemolyticus O3 : K6 and O4 : K68 serotypes has spread to Mozambique. Fifty-eight V. parahaemolyticus strains isolated from hospitalized d...

journal_title：Journal of medical microbiology

authors： Ansaruzzaman M,Chowdhury A,Bhuiyan NA,Sultana M,Safa A,Lucas M,von Seidlein L,Barreto A,Chaignat CL,Sack DA,Clemens JD,Nair GB,Choi SY,Jeon YS,Lee JH,Lee HR,Chun J,Kim DW

更新日期：2008-12-01 00:00:00

abstract：PURPOSE:This study aimed to explore daptomycin combined with fosfomycin or rifampin against the planktonic and adherent linezolid-resistant isolates of Enterococcus faecalis. METHODOLOGY:Four linezolid-resistant and four linezolid-sensitive isolates of E. faecalis which formed biofilms were collected for this study. B...

journal_title：Journal of medical microbiology

authors： Zheng JX,Sun X,Lin ZW,Qi GB,Tu HP,Wu Y,Jiang SB,Chen Z,Deng QW,Qu D,Yu ZJ

更新日期：2019-03-01 00:00:00

abstract：:A serial study of rotavirus infection in gnotobiotic piglets is described. They were infected when 7 days old and the course of infection was followed for 21 days, by immunofluorescence and histological examinations of the intestinal epithelium and by titration of the virus content of the gut lumen. There appeared to ...

journal_title：Journal of medical microbiology

authors： Crouch CF,Woode GN

更新日期：1978-08-01 00:00:00

abstract：:An ELISA was developed and evaluated as a method for detecting antibodies against glycosylated and deglycosylated histoplasmin (HMIN). Sera from patients with histoplasmosis, paracoccidioidomycosis, sporotrichosis, coccidioidomycosis, aspergillosis, cryptococcosis and healthy donors were tested by ELISA against purifi...

journal_title：Journal of medical microbiology

authors： Guimarães AJ,Pizzini CV,de Matos Guedes HL,Albuquerque PC,Peralta JM,Hamilton AJ,Zancopé-Oliveira RM

更新日期：2004-06-01 00:00:00

abstract：:Introduction.Candida auris is a pathogenic yeast that mainly affects immunosuppressed patients and those with implanted medical devices. This pathogen also displays elevated resistance to common antifungals and high survival and spreading capacities. Since no antifungal breakpoints have yet been defined for this patho...

journal_title：Journal of medical microbiology

authors： Romera D,Aguilera-Correa JJ,Gadea I,Viñuela-Sandoval L,García-Rodríguez J,Esteban J

更新日期：2019-09-01 00:00:00

abstract：:Several imipenem-susceptible and -resistant Aeromonas veronii biovar sobria isolates with different morphologies and antimicrobial susceptibilities recovered from bile samples of a patient with cholangitis were analysed. These isolates belonged to the same clone and the imipenem-resistant strains showed overexpression...

journal_title：Journal of medical microbiology

authors： Sánchez-Céspedes J,Figueras MJ,Aspiroz C,Aldea MJ,Toledo M,Alperí A,Marco F,Vila J

更新日期：2009-04-01 00:00:00

abstract：:Burkholderia pseudomallei is the causative agent of human and animal melioidosis. The role of quorum sensing (QS) in the in vivo pathogenicity of B. pseudomallei via inhalational exposure of BALB/c mice and intraperitoneal challenge of Syrian hamsters has not been reported. This investigation demonstrates that B. pseu...

journal_title：Journal of medical microbiology

authors： Ulrich RL,DeShazer D,Brueggemann EE,Hines HB,Oyston PC,Jeddeloh JA

更新日期：2004-11-01 00:00:00

abstract：:Two toxigenic Corynebacterium ulcerans isolates recovered from pharyngeal swabs of two patients from the same hospital in Japan during 2001-2002 were characterized by PFGE and ribotyping. Toxin production in different culture media was examined and serological analysis of patient sera was performed. The two isolates c...

journal_title：Journal of medical microbiology

authors： Komiya T,Seto Y,De Zoysa A,Iwaki M,Hatanaka A,Tsunoda A,Arakawa Y,Kozaki S,Takahashi M

更新日期：2010-12-01 00:00:00

abstract：:Synergy between fluvastatin, at clinically unachievable concentrations, and fluconazole against Candida albicans has been reported. The purpose of the present study was to evaluate the in-vitro activity of fluconazole alone and in combination with clinically achievable concentrations of pravastatin and fluvastatin aga...

journal_title：Journal of medical microbiology

authors： Nash JD,Burgess DS,Talbert RL

更新日期：2002-02-01 00:00:00

abstract：:The periodontopathic bacterium Actinobacillus actinomycetemcomitans has been implicated in the pathogenesis of periodontal diseases. It has been reported previously that infection with the organism induced apoptosis in the mouse macrophage cell line J774.1. In the present study, the role of caspases during apoptosis i...

journal_title：Journal of medical microbiology

authors： Okinaga T,Kasai H,Tsujisawa T,Nishihara T

更新日期：2007-10-01 00:00:00

abstract：:The growth inhibiting activity of transferrins, citrate, 2-2' dipyridyl and desferrioxamine methanesulphonate towards Legionella spp. and their serogroups was investigated. The inhibitory activity of all these compounds depended upon the iron-free state of the molecules and was abolished by saturation with iron. No ba...

journal_title：Journal of medical microbiology

authors： Goldoni P,Visca P,Pastoris MC,Valenti P,Orsi N

更新日期：1991-02-01 00:00:00

abstract：:The competition for glucose as a growth-limiting substrate between Candida albicans and a mixed community of oral bacteria was investigated. A chemostat was operated under glucose-limiting and glucose excess conditions at a dilution rate of 0.05/h. A mixed population of oral bacteria was established and after a steady...

journal_title：Journal of medical microbiology

authors： Basson NJ

更新日期：2000-11-01 00:00:00

abstract：:Introduction. The worldwide emergence of carbapenem resistance in Gram-negative bacteria makes the development of simple tests mandatory to identify antimicrobial resistance mechanisms. Enzymatic and membrane barriers are the prominent resistance mechanisms described in these bacteria. Several tests are currently used...

journal_title：Journal of medical microbiology

authors： Pinet E,Franceschi C,Collin V,Davin-Regli A,Zambardi G,Pagès JM

更新日期：2020-01-01 00:00:00

abstract：:Two chlamydial enzyme immunoassays, Chlamydiazyme and IDEIA, were evaluated in a mouse model of chlamydial genital-tract infection. The Chlamydiazyme assay and the IDEIA were assessed on specimens from 10 and 11 mice, respectively. The animals were infected with Chlamydia trachomatis, strain SA2f, and the results obta...

journal_title：Journal of medical microbiology

authors： Taylor-Robinson D,Tuffrey M

更新日期：1987-09-01 00:00:00

abstract：PURPOSE:Non-albicansCandida species have emerged as fungal pathogens that cause invasive infections, with many of these species displaying resistance to commonly used antifungal agents. This study was confined to studying the characteristics of clinical isolates of the C. rugosa complex and C. pararugosa species. METH...

journal_title：Journal of medical microbiology

authors： Peremalo T,Madhavan P,Hamzah S,Than L,Wong EH,Nasir MDM,Chong PP,Ng KP

更新日期：2019-03-01 00:00:00

abstract：:The purpose of this study was to determine optimal criteria for microbiology laboratory screening of endotracheal tube (ETT) specimens submitted for bacterial culture from adult patients. ETT specimens from adult patients that were received by two microbiology laboratories were prospectively evaluated and subdivided i...

journal_title：Journal of medical microbiology

authors： Walkty A,Lagacé-Wiens PRS,Manickam K,Adam H,Pieroni P,Alfa M,Karlowsky JA

更新日期：2012-09-01 00:00:00

abstract：:The acid end-products of 185 isolates from the family Bacteroidaceae were separated and analysed by gas-liquid chromatography on broth cultures. Different media were evaluated and definitive studies were performed in a fully supplemented complex medium. The limitations of this approach to the identification of a wide ...

journal_title：Journal of medical microbiology

authors： Deacon AG,Duerden BI,Holbrook WP

更新日期：1978-05-01 00:00:00

abstract：:A commercial method (RAPIDEC ur; bioMérieux UK Ltd, Basingstoke) and an in-house scheme based on conventional tests were compared for the identification of 179 commonly encountered gram-negative urinary pathogens. The methods identified correctly to genus level 97% and 84% of organisms, respectively. RAPIDEC ur was su...

journal_title：Journal of medical microbiology

authors： Batchelor BI

更新日期：1995-07-01 00:00:00

abstract：:Breast-fed infants are less susceptible to gastroenteritis than bottle-fed infants. Antibodies against rotavirus, the major pathogen of infantile gastroenteritis, were sought in human sera, colostrum and milk specimens by immunofluorescence. An experimental murine-rotavirus model was established by infecting the secon...

journal_title：Journal of medical microbiology

authors： Schoub BD,Prozesky OW,Lecatsas G,Oosthuizen R

更新日期：1978-02-01 00:00:00

abstract：:Immunoblotting sera from cases of Streptococcus mutans or S. sobrinus endocarditis against an extract from S. sobrinus strain MUCOB 263 had identified three immunodominant antigenic bands at 190, 200 and 220 kDa. A lambda ZAPII DNA library was produced from the sheared genomic DNA of S. sobrinus MUCOB 263 and six iden...

journal_title：Journal of medical microbiology

authors： Brooks W,Burnie JP

更新日期：1994-05-01 00:00:00

abstract：:A sandwich enzyme-linked immunosorbent assay (ELISA) with monoclonal antibody coupled to the solid phase was evaluated for the detection of brucella antigen in serum samples. Under optimum conditions, 100 brucella cells/well or 10(5) fg/ml of lipopolysaccharide (LPS) were detected in spiked specimens. The standardised...

journal_title：Journal of medical microbiology

authors： Al-Shamahy HA,Wright SG

更新日期：1998-02-01 00:00:00

abstract：:Community-associated meticillin-resistant Staphylococcus aureus (CA-MRSA) have emerged globally and have been associated with more severe disease than healthcare-associated MRSA (HA-MRSA). The purpose of this study was to determine whether laboratory measures of virulence can distinguish dominant CA-MRSA clones from H...

journal_title：Journal of medical microbiology

authors： Boakes E,Marbach H,Lynham S,Ward M,Edgeworth JD,Otter JA

更新日期：2016-12-01 00:00:00

abstract：:Candida albicans, as an opportunistic pathogen, can cause superficial and life-threatening candidiasis in immunocompromised individuals. The formation of surface-associated biofilms and the appearance of drug resistance pose a significant challenge for clinical intervention. In this study, a total of 104 hospital-acqu...

journal_title：Journal of medical microbiology

authors： Hu L,Du X,Li T,Song Y,Zai S,Hu X,Zhang X,Li M

更新日期：2015-01-01 00:00:00