Gas-lipuid chromatographic analysis of metabolic products in the identification of bacteroidaceae of clinical interest.

abstract：:Stenotrophomonas maltophilia is an opportunist multidrug-resistant pathogen that causes a wide range of nosocomial infections. Various cystic fibrosis (CF) centres have reported an increasing prevalence of S. maltophilia colonization/infection among patients with this disease. The purpose of this study was to assess s...

journal_title：Journal of medical microbiology

authors： Vidigal PG,Mosel F,Koehling HL,Mueller KD,Buer J,Rath PM,Steinmann J

更新日期：2014-12-01 00:00:00

abstract：PURPOSE:We previously identified an association between CC22 meticillin-resistant Staphylococcus aureus (MRSA) bloodstream infection isolates with an elevated vancomycin MIC (V-MIC) in the susceptible range (1.5-2 mg l-1) and endocarditis. This study explores whether these isolates have a specific phenotype consistent ...

journal_title：Journal of medical microbiology

authors： Marbach H,Boakes E,Lynham S,Ward M,Otter JA,Edgeworth JD

更新日期：2017-05-01 00:00:00

abstract：:Different aspects of the terms strain, clone and species are discussed. The term strain is commonly used to denote a pure culture - here called 'the strain in the taxonomic sense' - but does also refer to a natural concept closely related to the clone. The term clone on the other hand is used both in a general and in ...

journal_title：Journal of medical microbiology

authors： Dijkshoorn L,Ursing BM,Ursing JB

更新日期：2000-05-01 00:00:00

abstract：:A novel direct detection system has been developed for eight staphylococcal enterotoxin (SE)-encoding genes (sea, seb, sec, sed, see, seg, seh and sei) in milk. Specific detection by real-time PCR was successful for all SE-encoding genes in the reference strains. Furthermore, a novel DNA-preparation method with good r...

journal_title：Journal of medical microbiology

authors： Nakayama A,Okayama A,Hashida M,Yamamoto Y,Takebe H,Ohnaka T,Tanaka T,Imai S

更新日期：2006-03-01 00:00:00

abstract：PURPOSE:The current study has been designed to delineate the efficacy of geraniol (GE) on biofilm formation in Staphylococcus epidermidis as well as the effect of subinhibitory concentrations of GE on the development of adaptive resistance. METHODOLOGY:Biofilm biomass quantification assay was performed to evaluate the...

journal_title：Journal of medical microbiology

authors： Kannappan A,Sivaranjani M,Srinivasan R,Rathna J,Pandian SK,Ravi AV

更新日期：2017-10-01 00:00:00

abstract：:A male patient of 32 years was referred for surgical drainage and orchidectomy of the right testis following a cycling injury. A Venereal Disease Research Laboratory (VDRL) test was requested by the surgery department to rule out secondary syphilis. Although serum samples gave a negative result in the VDRL test, quali...

journal_title：Journal of medical microbiology

authors： Purwar S,Metgud SC,Gokale SK

更新日期：2012-03-01 00:00:00

abstract：:The ELISA technique was shown to be group-specific for the detection of IgM antibodies against coxsackie B viruses, and probably against a wider range of enteroviruses. No evidence was obtained that recent coxsackie B-virus infection predisposes to myocardial infarction. ...

journal_title：Journal of medical microbiology

authors： Hannington G,Booth JC,Bowes RJ,Stern H

更新日期：1986-06-01 00:00:00

abstract：:The aim of the present study was to correlate molecular evidence of the presence of Helicobacter pylori in gastric biopsy samples, based on analysis of 16S rDNA, vacuolating toxin (vacA), urease A (ureA) and cagA genes, with the clinical, histological and serological findings in patients with H. pylori-associated gast...

journal_title：Journal of medical microbiology

authors： Tiveljung A,Borch K,Jonasson J,Mårdh S,Petersson F,Monstein HJ

更新日期：1998-08-01 00:00:00

abstract：:Mycoplasma fermentans has attracted much interest both as a cofactor for the progression of AIDS and as a pathogenic agent in non-AIDS related diseases. Previous studies with serological and genetic techniques suggest that M. fermentans represents a homogeneous group of organisms, with no significant differences ident...

journal_title：Journal of medical microbiology

authors： Hannan PC,Kearns AM,Sisson PR,Freeman R

更新日期：1997-04-01 00:00:00

abstract：:Serology remains the mainstay for diagnosis of Epstein-Barr virus (EBV) infection. This study compared two automated platforms (BioPlex 2200 and Architect i2000SR) to test three EBV serological markers: viral capsid antigen (VCA) immunoglobulins of class M (IgM), VCA immunoglobulins of class G (IgG) and EBV nuclear an...

journal_title：Journal of medical microbiology

authors： Al Sidairi H,Binkhamis K,Jackson C,Roberts C,Heinstein C,MacDonald J,Needle R,Hatchette TF,LeBlanc JJ

更新日期：2017-11-01 00:00:00

abstract：:Six-week-old lambs were inoculated orally with 10(9) cfu of an antibiotic-resistance marked four-strain mixture of enterohaemorrhagic Escherichia coli (EHEC) O157:H7 to investigate faecal excretion and intestinal colonisation. In the first experiment, three E. coli O157:H7 isolates were not detected in the faeces of a...

journal_title：Journal of medical microbiology

authors： Cookson AL,Wales AD,Roe JM,Hayes CM,Pearson GR,Woodward MJ

更新日期：2002-12-01 00:00:00

abstract：:A total of 110 clinical isolates of Vibrio cholerae O1 biotype El Tor serotype Ogawa isolated in a recent outbreak from different districts of Teheran, Iran, was subjected to 99 carbon source utilisation tests, ribotyping and toxinogenotyping. PCR showed that the genes encoding cholera toxin (ctxA), toxin co-regulated...

journal_title：Journal of medical microbiology

authors： Pourshafie MR,Grimont F,Saifi M,Grimont PAD

更新日期：2000-12-01 00:00:00

abstract：:The microtechnique of Galton et al. (1965) for the routine screening of blood samples for leptospiral antibodies was used to examine more than 8000 serum samples from animals that had aborted and from herds in which leptospiral antibodies had previously been demonstrated. The method gave consistently accurate results....

journal_title：Journal of medical microbiology

authors： Lewis J

更新日期：1978-08-01 00:00:00

abstract：:The unpredictable characteristics of meningococcal disease (MD) make outbreaks complicated to monitor and consequently lead to high levels of public anxiety. Traditional molecular techniques have been utilized in order to understand better the epidemiology of MD, but some have disadvantages such as being highly specia...

journal_title：Journal of medical microbiology

authors： Diggle MA,Bell CM,Clarke SC

更新日期：2003-06-01 00:00:00

abstract：:The production of toxins A and B by Clostridium difficile was greatly enhanced under biotin-limited conditions, in which a 140-kDa protein was expressed strongly. Gene cloning revealed that this protein was a homologue of formylglycinamidine ribonucleotide synthetase (FGAM synthetase, EC 6.3.5.3), which is known as Pu...

journal_title：Journal of medical microbiology

authors： Maegawa T,Karasawa T,Ohta T,Wang X,Kato H,Hayashi H,Nakamura S

更新日期：2002-01-01 00:00:00

abstract：PURPOSE:Membrane fluidity to a large extent is governed by the presence of branched-chain fatty acids (BCFAs). Branched-chain α-keto acid dehydrogenase (BKD) is the key enzyme in BCFA synthesis. A Staphylococcus aureus BKD-deficient strain still produced substantial levels of BCFAs. Pyruvate dehydrogenase (PDH) with st...

journal_title：Journal of medical microbiology

authors： Singh VK,Sirobhushanam S,Ring RP,Singh S,Gatto C,Wilkinson BJ

更新日期：2018-04-01 00:00:00

abstract：:A novel spirochaete was isolated from a case of severe virulent ovine foot rot (SVOFR) by immunomagnetic separation with beads coated with polyclonal anti-treponemal antisera and prolonged anaerobic broth culture. The as yet unnamed treponeme differs considerably from the only other spirochaete isolated from ovine foo...

journal_title：Journal of medical microbiology

authors： Demirkan I,Carter SD,Winstanley C,Bruce KD,McNAIR NM,Woodside M,Hart CA

更新日期：2001-12-01 00:00:00

abstract：PURPOSE:Catheter-related bloodstream infections (CRBSIs) are among the most common hospital-acquired infections. We aimed to survey methicillin resistance, biofilm production and susceptibility to vancomycin, linezolid and other antibiotics for staphylococci isolated from CRBSIs. METHODOLOGY:Fifty-eight isolates [20 S...

journal_title：Journal of medical microbiology

authors： Hashem AA,Abd El Fadeal NM,Shehata AS

更新日期：2017-06-01 00:00:00

abstract：:Chemotaxis of polymorphonuclear leukocytes (PMNL) in response to cell components of Bacteroides fragilis alone or in combination with Escherichia coli was evaluated. E. coli produced much more powerful chemotactic factors than B. fragilis. The culture filtrate (CF), outer membrane (OM) preparation, and lipopolysacchar...

journal_title：Journal of medical microbiology

authors： Namavar F,Verweij-Van Vught AM,Bal M,MacLaren DM

更新日期：1987-09-01 00:00:00

abstract：:In this study, 90 non-replicate imipenem-resistant Pseudomonas aeruginosa (IRPA) Malaysian isolates collected between October 2005 and March 2008 were subjected to a screening test for detection of the integron and the gene cassette. Class 1 integrons were detected in 54 IRPA clinical isolates, whilst three isolates c...

journal_title：Journal of medical microbiology

authors： Khosravi Y,Tay ST,Vadivelu J

更新日期：2011-07-01 00:00:00

abstract：:The acute dental abscess is frequently underestimated in terms of its morbidity and mortality. The risk of potential serious consequences arising from the spread of a dental abscess is still relevant today with many hospital admissions for dental sepsis. The acute dental abscess is usually polymicrobial comprising fac...

journal_title：Journal of medical microbiology

authors： Robertson D,Smith AJ

更新日期：2009-02-01 00:00:00

abstract：:The main components of oral malodour have been identified as volatile sulfur compounds (VSCs), including hydrogen sulfide (H(2)S) and methyl mercaptan (CH(3)SH). The lactoperoxidase (LPO) system (consisting of LPO, glucose oxidase, glucose and thiocyanate) was previously shown to exhibit antimicrobial activities again...

journal_title：Journal of medical microbiology

authors： Nakano M,Shin K,Wakabayashi H,Yamauchi K,Abe F,Hironaka S

更新日期：2015-10-01 00:00:00

abstract：:Breast-fed infants are less susceptible to gastroenteritis than bottle-fed infants. Antibodies against rotavirus, the major pathogen of infantile gastroenteritis, were sought in human sera, colostrum and milk specimens by immunofluorescence. An experimental murine-rotavirus model was established by infecting the secon...

journal_title：Journal of medical microbiology

authors： Schoub BD,Prozesky OW,Lecatsas G,Oosthuizen R

更新日期：1978-02-01 00:00:00

abstract：:The purpose of this study was to compare the frequency of recovery of aerobic and anaerobic organisms with interfering capability against group A beta-haemolytic streptococci (GABHS) and beta-lactamase-producing bacteria (BLPB) from the tonsils of GABHS carriers and non-carriers. The presence of aerobic and anaerobic ...

journal_title：Journal of medical microbiology

authors： Brook I,Gober AE

更新日期：2006-12-01 00:00:00

abstract：:Bacterial activity and growth were monitored by following the changes of electrical impedance of cultures in liquid media. The signal is expressed automatically as a curve similar to grwoth curves produced by other methods. The technique offers a new, rapid and sensitive means of detecting active micro-organisms and i...

journal_title：Journal of medical microbiology

authors： Ur A,Brown DF

更新日期：1975-02-01 00:00:00

abstract：:In serological typing of Klebsiella pneumoniae strains from human, equine and environmental sources, the capsular identity of many isolates could not be determined because of serological cross-reactivity. A panel of 91 bacteriophages able to lyse each of the 77 capsular serotypes of K. pneumoniae was isolated and test...

journal_title：Journal of medical microbiology

authors： Pieroni P,Rennie RP,Ziola B,Deneer HG

更新日期：1994-12-01 00:00:00

abstract：:Clearance of various bacteria isolated from portal and systemic blood of mice was evaluated and compared. All portal blood strains, including Escherichia coli and enterococci were eliminated more rapidly from the circulation than were strains isolated from systemic blood, including Pseudomonas aeruginosa. With mannose...

journal_title：Journal of medical microbiology

authors： Hirakata Y,Kaku M,Furuya N,Matsumoto T,Tateda K,Tomono K,Yamaguchi K

更新日期：1993-05-01 00:00:00

abstract：:In this study the importance of Providencia species as a cause of travellers' diarrhoea was examined using a selective medium developed by the authors. Providencia species could easily be distinguished from other enteric pathogens by the colour of the colonies obtained. Nine strains of Providencia alcalifaciens, nine ...

journal_title：Journal of medical microbiology

authors： Yoh M,Matsuyama J,Ohnishi M,Takagi K,Miyagi H,Mori K,Park KS,Ono T,Honda T

更新日期：2005-11-01 00:00:00

abstract：:DNA hybridisation of 309 consecutive Staphylococcus aureus clinical isolates with oligonucleotide probes specific for genes encoding Panton-Valentine leucocidin (luk-PV) and gamma-haemolysin (hlg) revealed that 99% of randomly selected strains carried the hlg locus whereas only 2% harboured the luk-PV as well as the h...

journal_title：Journal of medical microbiology

authors： Prevost G,Couppie P,Prevost P,Gayet S,Petiau P,Cribier B,Monteil H,Piemont Y

更新日期：1995-04-01 00:00:00

abstract：:The aim of this work was to evaluate the effects of beneficial human vaginal lactobacilli (Lb) on urogenital pathogens through in vitro and in vivo experiments. Co-aggregative and antimicrobial properties between five vaginal Lb strains and urogenital pathogens or potential pathogens (Streptococcus agalactiae, Staphyl...

journal_title：Journal of medical microbiology

authors： De Gregorio PR,Tomás MSJ,Terraf MCL,Nader-Macías MEF

更新日期：2014-05-01 00:00:00