Inactivating effects of the lactoperoxidase system on bacterial lyases involved in oral malodour production.

Abstract:

:The main components of oral malodour have been identified as volatile sulfur compounds (VSCs), including hydrogen sulfide (H(2)S) and methyl mercaptan (CH(3)SH). The lactoperoxidase (LPO) system (consisting of LPO, glucose oxidase, glucose and thiocyanate) was previously shown to exhibit antimicrobial activities against some oral bacteria in vitro and suppressive effects on VSCs in mouth air in a clinical trial. Here, we examined the in vitro effects of the LPO system on the activities of the bacterial lyases involved in the production of VSCs by oral anaerobes. The exposure of crude bacterial extracts of Fusobacterium nucleatum and Porphyromonas gingivalis or purified methionine γ-lyase to the LPO system resulted in the inactivation of their lyase activities through l-cysteine and l-methionine, which was linked to the production of H(2)S and CH(3)SH, respectively. The exposure of living F. nucleatum and P. gingivalis cells to the LPO system resulted in the suppression of cell numbers and lyase activities. The inactivation of the crude bacterial extracts of F. nucleatum and purified methionine γ-lyase by the LPO system was partly recovered by the addition of DTT. Therefore, the LPO system may inactivate bacterial lyases including methionine γ-lyase by reacting with the free cysteine residues of lyases. These results suggested that the LPO system suppresses the production of VSCs not only through its antimicrobial effects, but also by its inactivating effects on the bacterial lyases of F. nucleatum and P. gingivalis.

journal_name

J Med Microbiol

authors

Nakano M,Shin K,Wakabayashi H,Yamauchi K,Abe F,Hironaka S

doi

10.1099/jmm.0.000150

subject

Has Abstract

pub_date

2015-10-01 00:00:00

pages

1244-1252

issue

10

eissn

0022-2615

issn

1473-5644

journal_volume

64

pub_type

杂志文章
  • Immunological alterations during the clinical and recovery phases of experimental swine dysentery.

    abstract::The aim of this study was to examine changes in the systemic immune response during the incubation period and following the onset of clinical swine dysentery, including the recovery period. Ten healthy conventional pigs were inoculated with Brachyspira hyodysenteriae. Blood was sampled at pre-inoculation, at days 4 an...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46538-0

    authors: Jonasson R,Andersson M,Råsbäck T,Johannisson A,Jensen-Waern M

    更新日期:2006-07-01 00:00:00

  • Molecular epidemiology of Bordetella pertussis in Greece, 2010-2015.

    abstract:PURPOSE:To determine the predominant strains of Bordetella pertussis in Greece during 2010-2015. METHODOLOGY:Infants and children (n=1150) (15 days to 14 years) of Greek, Roma and immigrant origin with different vaccination statuses were hospitalized in Athens, Greece with suspected pertussis infection. IS481/IS1001 r...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000688

    authors: Petridou E,Jensen CB,Arvanitidis A,Giannaki-Psinaki M,Michos A,Krogfelt KA,Petersen RF

    更新日期:2018-03-01 00:00:00

  • Analysis of Epstein-Barr virus and cellular gene expression during the early phases of Epstein-Barr virus lytic induction.

    abstract::In order to develop novel host/pathogen real-time PCR assays for routine diagnostic use, early gene expression patterns from both Epstein-Barr virus (EBV) and Raji cells were examined after inducing the lytic life cycle using 12-O-tetradecanoyl-13-phorbol ester and sodium butyrate. Real-time PCR identified several hig...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000352

    authors: Auburn H,Zuckerman M,Smith M

    更新日期:2016-11-01 00:00:00

  • Spread of the Brazilian epidemic clone of a multiresistant MRSA in two cities in Argentina.

    abstract::Methicillin-resistant Staphylococcus aureus (MRSA) is recognised as an important cause of nosocomial infection. The spread of some MRSA epidemic clones is well documented. In Brazil, and more recently in Portugal, a considerable number of hospital infections has been caused by a unique multiresistant MRSA clone design...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-49-2-187

    authors: Coimbra MVDS,Teixeira LA,Ramos RLB,Predari SC,Castello L,Famiglietti A,Vay C,Klan L,Figueiredo AMS

    更新日期:2000-02-01 00:00:00

  • Comparative analysis of 16S RNA nucleotide sequences of Anaplasma phagocytophilum detected in the blood of horses from various parts of Europe.

    abstract::The aim of this study was to conduct a comparative analysis of the 16S rRNA gene fragment nucleotide sequences for Anaplasma phagocytophilum strains detected in the blood of horses from various parts of Europe. The study comprised 234 horses that had had contact with ticks. Using PCR, the genetic material of A. phagoc...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.058636-0

    authors: Dzięgiel B,Adaszek L,Winiarczyk M,García-Bocanegra I,Carbonero A,Dębiak P,Winiarczyk S

    更新日期:2013-12-01 00:00:00

  • Cell damage caused by vaginal Candida albicans isolates from women with different symptomatologies.

    abstract::The present study aimed to characterize cell damage caused by vaginal Candida albicans isolates from women with different symptomatologies. It was evaluated 12 clinical isolates of C. albicans from vaginal samples: 4 from asymptomatic women (AS), 4 from women with a single episode of vulvovaginal candidiasis (VVC) and...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000547

    authors: Faria DR,Sakita KM,Akimoto-Gunther LS,Kioshima ÉS,Svidzinski TIE,Bonfim-Mendonça PS

    更新日期:2017-08-01 00:00:00

  • Variants of Shiga-like toxin II constitute a major toxin component in Escherichia coli O157 strains from patients with haemolytic uraemic syndrome.

    abstract::The prevalence and genotype of Shiga-like toxins (SLTs) in Escherichia coli (O)157 strains from patients in Germany with haemolytic uraemic syndrome (HUS) were investigated. This was done by PCR amplification of the B-subunit genes with two primer pairs--one complementary to slt-IB, and the other homologous to both sl...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-40-5-338

    authors: Rüssmann H,Schmidt H,Heesemann J,Caprioli A,Karch H

    更新日期:1994-05-01 00:00:00

  • Antifungal activity of interleukin-2-activated natural killer (NK1.1+) lymphocytes against Candida albicans.

    abstract::Interleukin-2 (IL-2)-activated lymphocytes interact directly with, and inhibit, the growth of Candida albicans hyphae. C. albicans-stimulated natural killer (NK1.1+) lymphocytes were demonstrated to secrete a soluble product capable of directly affecting C. albicans yeast forms. Antibodies specific for interferon-gamm...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-47-11-1007

    authors: Mathews HL,Witek-Janusek L

    更新日期:1998-11-01 00:00:00

  • Lesions produced by Clostridium butyricum strain CB 1002 in ligated intestinal loops in guinea pigs.

    abstract::Heated spores (80 degrees C, 10 min) of Clostridium butyricum strain CB 1002 isolated from a fatal case of necrotising enterocolitis in a human neonate were inoculated into ligated intestinal loops prepared in young conventional guinea pigs. Necropsy findings 18 h later included congestion, patchy haemorrhage of the i...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-19-3-351

    authors: Popoff MR,Ravisse P

    更新日期:1985-06-01 00:00:00

  • Emerging multidrug resistance isolates of hospital-acquired bacterial meningitis in a tertiary care centre in North India.

    abstract::Purpose. Acute bacterial meningitis continues to be a potentially life threatening condition. Hospital-acquired meningitis is rapidly increasing and adding an immense burden to the health system due to the emergence of multidrug resistance isolates. The purpose of this study is to find the antibiotic susceptibility pa...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001072

    authors: Raza MS,Das BK,Goyal V,Lodha R,Chaudhry R,Sood S,Gautam H,Sreenivas V,Nair D,Mohapatra S,Kapil A

    更新日期:2019-11-01 00:00:00

  • Agglutination of "Streptococcus milleri" by lectins.

    abstract::The agglutination of 218 clinical isolates and three ATCC type strains of "Streptococcus milleri" was tested with 25 different lectins from plants and fungi. An agglutination reaction with one or more lectins was observed with 42 isolates when the cells were untreated. After trypsinisation of the bacteria, 109 strains...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-41-1-14

    authors: Kellens JT,Jacobs JA,Peumans WJ,Stobberingh EE

    更新日期:1994-07-01 00:00:00

  • Molecular epidemiological study of Vibrio cholerae isolates from infected patients in Teheran, Iran.

    abstract::A total of 110 clinical isolates of Vibrio cholerae O1 biotype El Tor serotype Ogawa isolated in a recent outbreak from different districts of Teheran, Iran, was subjected to 99 carbon source utilisation tests, ribotyping and toxinogenotyping. PCR showed that the genes encoding cholera toxin (ctxA), toxin co-regulated...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-49-12-1085

    authors: Pourshafie MR,Grimont F,Saifi M,Grimont PAD

    更新日期:2000-12-01 00:00:00

  • Resistance to desiccation and skin fatty acids in outbreak strains of methicillin-resistant Staphylococcus aureus.

    abstract::Resistance to desiccation and to skin fatty acids was measured in three groups of methicillin-resistant Staphylococcus aureus (MRSA) strains and a group of control strains. Organisms from a large outbreak on a special care baby unit (SCBU), where MRSA had been isolated from staff hands but not from the environment, we...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-36-1-56

    authors: Farrington M,Brenwald N,Haines D,Walpole E

    更新日期:1992-01-01 00:00:00

  • Seroprevalence of Chlamydia infection in pigs in Jiangxi province, South-Eastern China.

    abstract::Chlamydia are Gram-negative obligate bacteria that cause a wide range of diseases in humans and animals. To assess the risk of zoonosis posed by pigs, a total of 920 serum samples were collected from pigs in 11 administrative cities in Jiangxi province, south-eastern China, and the seroprevalence of Chlamydia antibodi...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.066555-0

    authors: Jiang HH,Huang SY,Zhang WB,Zhao L,Xu CM,Deng SZ,Zhu XQ

    更新日期:2013-12-01 00:00:00

  • Effect of glucan on Leishmania major infection in BALB/c mice.

    abstract::The effect of glucan on Leishmania major infection was studied in BALB/c mice, which are highly susceptible to leishmania infection. Glucan (0.45 mg), or isovolumetric dextrose, was administered intraperitoneally 7, 5, 3 and 1 day before infection with L. major promastigotes. At 3, 5, 6, 8 and 10 weeks after infection...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-23-4-363

    authors: Al Tuwaijri AS,Mahmoud AA,Al Mofleh IA,Al Khuwaitir SA

    更新日期:1987-06-01 00:00:00

  • Langerhans cell density and serological changes following intradermal immunisation of mice with dengue 2 virus.

    abstract::After the introduction of the dengue-2 (16681) virus by intradermal (i.d.) injection into the footpads of mice, Langerhans cells (LCs) increased in numbers within 24 h at the site of injection and neutralising antibody developed. On comparing the i.d. and intramuscular (i.m.) routes, antibody was produced more rapidly...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-45-2-138

    authors: Taweechaisupapong S,Sriurairatana S,Angsubhakorn S,Yoksan S,Khin MM,Sahaphong S,Bhamarapravati N

    更新日期:1996-08-01 00:00:00

  • Antibacterial activity of Pinus elliottii and its major compound, dehydroabietic acid, against multidrug-resistant strains.

    abstract::Antibiotic-resistant bacteria have emerged from the widespread use of antibiotics worldwide and have prompted the search for new sources of antimicrobial substances. Pinus spp. contain several bioactive compounds consisting mainly of terpenes, terpenoids and some other aromatic and aliphatic constituents. These compou...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.081711-0

    authors: Leandro LF,Cardoso MJO,Silva SDC,Souza MGM,Veneziani RCS,Ambrosio SR,Martins CHG

    更新日期:2014-12-01 00:00:00

  • Laboratory-based surveillance of human verocytotoxigenic Escherichia coli infection in the Republic of Ireland, 2002-2004.

    abstract::The aim of this study was to examine the frequency and distribution of human verocytotoxigenic Escherichia coli (VTEC) O157 and non-O157 in the Republic of Ireland, and also to examine the presence of virulence genes in these isolates. This genetic information combined with phenotypic tests was used to produce a compl...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46147-0

    authors: Carroll AM,Gibson A,McNamara EB

    更新日期:2005-12-01 00:00:00

  • Carriage of inhibitor-producing organisms on human skin.

    abstract::The carriage on skin lesions, on healthy skin and in the nose, of micro-organisms that produce antibiotics active against Staphylococcus aureus has been studied. About 5% of sites (10% of all patients) yielded such organisms. No evidence could be found that the presence of producer organisms in skin lesions had a prot...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-13-2-329

    authors: Noble WC,Willie JA

    更新日期:1980-05-01 00:00:00

  • Pertussis epidemiology in Tunisian infants and children and characterization of Bordetella pertussis isolates: results of a 9-year surveillance study, 2007 to 2016.

    abstract:PURPOSE:Pertussis remains a public health concern in most countries. Our study aimed to prospectively explore the epidemiology of pertussis in the Tunis area of Tunisia between 2007 and 2016, and to characterize the virulence-associated genes of the collected Bordetella pertussis isolates. METHODOLOGY:Infants and chil...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000892

    authors: Ben Fraj I,Kechrid A,Guillot S,Bouchez V,Brisse S,Guiso N,Smaoui H

    更新日期:2019-02-01 00:00:00

  • Differentiation of strains of Mycoplasma fermentans from various sources by pyrolysis mass spectrometry.

    abstract::Mycoplasma fermentans has attracted much interest both as a cofactor for the progression of AIDS and as a pathogenic agent in non-AIDS related diseases. Previous studies with serological and genetic techniques suggest that M. fermentans represents a homogeneous group of organisms, with no significant differences ident...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-46-4-348

    authors: Hannan PC,Kearns AM,Sisson PR,Freeman R

    更新日期:1997-04-01 00:00:00

  • Lemierre's syndrome and septicaemia caused solely by Arcanobacterium haemolyticum in a young immunocompetent patient.

    abstract::We present a case of septicaemia caused by Arcanobacterium haemolyticum in a previously healthy 23-year-old man suffering from acute pharyngotonsillitis, who developed complicated Lemierre's syndrome. Three blood cultures (both aerobic and anaerobic) revealed the exclusive presence of A. haemolyticum. The presence of ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.007492-0

    authors: Fernández-Suárez A,Aguilar Benítez JM,López Vidal AM,Díaz Iglesias JM

    更新日期:2009-12-01 00:00:00

  • Nasal carriage of Staphylococcus aureus in a population of healthy Nigerian students.

    abstract::The nasal carrier-rate of Staphylococcus aureus in 548 Nigerians aged 9-32 years and attending various educational establishments was 56.4%. This rate decreased with increasing age. A significantly greater proportion of females (65.0%) than males (46.5%) were carriers, but the excess in females was apparent only in su...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-19-2-211

    authors: Lamikanra A,Paul BD,Akinwole OB,Paul MO

    更新日期:1985-04-01 00:00:00

  • Epigallocatechin gallate suppresses expression of receptor activator of NF-kappaB ligand (RANKL) in Staphylococcus aureus infection in osteoblast-like NRG cells.

    abstract::Catechin, a constituent of tea, possesses various bioactivities. In particular, the most abundant catechin in tea is epigallocatechin gallate (EGCg), which has an anti-inflammatory effect. In the present study, the usability of EGCg for osteomyelitis treatment was examined. Osteomyelitis is a difficult disease to cure...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.47029-0

    authors: Ishida I,Kohda C,Yanagawa Y,Miyaoka H,Shimamura T

    更新日期:2007-08-01 00:00:00

  • Comparative genome and evolution analysis of the locus of enterocyte effacement from enteropathogenic Escherichia coli Deng and its transcriptional response to ciprofloxacin.

    abstract:PURPOSE:In this study, we aimed to investigate the genomic characteristics and evolution of pathogenicity islands of an enteropathogenic Escherichia coli (EPEC) strain, and to obtain a transcriptional profile of EPEC under different concentrations of ciprofloxacin using microarray analysis. METHODOLOGY:The complete EP...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000790

    authors: Chen Z,Cheng H,Pan W,Zheng J,Li D,Lin F,Yu Z,Deng Q

    更新日期:2018-09-01 00:00:00

  • Restoring the activity of the antibiotic aztreonam using the polyphenol epigallocatechin gallate (EGCG) against multidrug-resistant clinical isolates of Pseudomonas aeruginosa.

    abstract::Introduction. Pseudomonas aeruginosa is an important Gram-negative pathogen that is intrinsically multidrug-resistant (MDR) and frequently associated with healthcare-associated outbreaks. With increasing resistance to antibiotics and with very few novel drugs under development, clinicians often use combinations to tre...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001060

    authors: Betts JW,Hornsey M,Higgins PG,Lucassen K,Wille J,Salguero FJ,Seifert H,La Ragione RM

    更新日期:2019-10-01 00:00:00

  • Application of terminal RFLP analysis to characterize oral bacterial flora in saliva of healthy subjects and patients with periodontitis.

    abstract::Terminal restriction fragment-length polymorphism (T-RFLP) analysis was applied to characterize oral bacterial flora in saliva from 18 healthy subjects and 18 patients with periodontitis. The 16S rRNA genes (rDNAs) of oral bacteria and spirochaetes in saliva were amplified by PCR with a 6'carboxy-fluorescein (6-FAM)-l...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.04991-0

    authors: Sakamoto M,Takeuchi Y,Umeda M,Ishikawa I,Benno Y

    更新日期:2003-01-01 00:00:00

  • Multi-drug resistant Vibrio cholerae O1 variant El Tor isolated in northern Vietnam between 2007 and 2010.

    abstract::Since 2007, there has been a re-emergence of cholera outbreaks in northern Vietnam. To understand the molecular epidemiological relatedness and determine the antibiotic susceptibility profiles of responsible V. cholerae O1 outbreak strains, a representative collection of 100 V. cholerae O1 strains was characterized. V...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.034744-0

    authors: Tran HD,Alam M,Trung NV,Van Kinh N,Nguyen HH,Pham VC,Ansaruzzaman M,Rashed SM,Bhuiyan NA,Dao TT,Endtz HP,Wertheim HFL

    更新日期:2012-03-01 00:00:00

  • 5-Fluorouracil sensitivity varies among oral micro-organisms.

    abstract::5-Fluorouracil (5-FU), a commonly used chemotherapeutic agent, often causes oral mucositis, an inflammation and ulceration of the oral mucosa. Micro-organisms in the oral cavity are thought to play an important role in the aggravation and severity of mucositis, but the mechanisms behind this remain unclear. Although 5...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000292

    authors: Vanlancker E,Vanhoecke B,Smet R,Props R,Van de Wiele T

    更新日期:2016-08-01 00:00:00

  • Coxsackie B virus-specific IgM antibody and myocardial infarction.

    abstract::The ELISA technique was shown to be group-specific for the detection of IgM antibodies against coxsackie B viruses, and probably against a wider range of enteroviruses. No evidence was obtained that recent coxsackie B-virus infection predisposes to myocardial infarction. ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-21-4-287

    authors: Hannington G,Booth JC,Bowes RJ,Stern H

    更新日期:1986-06-01 00:00:00