Abstract：
:Bacterial activity and growth were monitored by following the changes of electrical impedance of cultures in liquid media. The signal is expressed automatically as a curve similar to grwoth curves produced by other methods. The technique offers a new, rapid and sensitive means of detecting active micro-organisms and is potentially the basis of rapid automated systems in this field. The impedance changes indicate that the micro-organisms metabolise substrates of low conductivity into products of high conductivity and that the changes are due to the activity of the micro-organisms rather than increase in their numbers. The activity of strains of Escherichia coli, Klebsiella aerogenes, Pseudo-monas aeruginosa, Staphyloccus aureus, and Streptococcus faecalis was detected within 2 h with inocula of 10-3 minus 10-5 organisms per ml. Different reactions of bacteria in various media suggest that the method may be applied to the rapid identification of micro-organisms. The inhibitory effect of antibiotics on bacteria was demonstrated within 2 h, indicating that the method may be useful for the rapid determination of bacterial sensitivity to antibiotics and the rapid assay of antibiotics in serum. Correlation of response time to initial inoculum allows estimatin of numbers of viable organisms. The sensitivity of the method allowed detection of activity due to Myoplasma argininii within 3 h; this suggests that the method might be applicable to the rapid detection of other slowly growing organisms, such as mycobacteria.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Ur A,Brown DFdoi
10.1099/00222615-8-1-19subject
Has Abstractpub_date
1975-02-01 00:00:00pages
19-28issue
1eissn
0022-2615issn
1473-5644journal_volume
8pub_type
杂志文章abstract：:Invasive infections caused by Brevundimonas vesicularis are very rare in humans. We experienced an unusual case of liver abscess due to B. vesicularis in an immunocompetent young male. The patient was successfully treated by liver abscess drainage and with antimicrobial therapy of ceftriaxone followed by ampicillin/su...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.045120-0
更新日期：2012-10-01 00:00:00
abstract：PURPOSE:Penicillium is the most common mould isolated in housing. Penicillium chrysogenum is the only species tested by prick test or serology for allergic patients. The American Institute of Medicine has accepted Penicillium as an aetiological agent of rhinitis in children and adults and as an asthma agent in children...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000960
更新日期：2019-05-01 00:00:00
abstract：:A large molecular-weight fraction of Pseudomonas aeruginosa culture filtrate protected rats and mice against a lethal infection with a heterologous serotype, and to some extent against Escherichia coli and Listeria monocytogenes. The active components were obtained from cultures grown for several days in a simple synt...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-13-2-201
更新日期：1980-05-01 00:00:00
abstract：PURPOSE:Non-albicansCandida species have emerged as fungal pathogens that cause invasive infections, with many of these species displaying resistance to commonly used antifungal agents. This study was confined to studying the characteristics of clinical isolates of the C. rugosa complex and C. pararugosa species. METH...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000940
更新日期：2019-03-01 00:00:00
abstract：:Subtractive hybridisation was used to screen for and identify conserved DNA sequences associated with clinical, clonal populations of Staphylococcus aureus. DNA from S. aureus strain ISP8 (a clinical isolate) was digested with TaqI and hybridised with randomly fragmented pooled DNA from 10 non-clinical (community) iso...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-46-12-987
更新日期：1997-12-01 00:00:00
abstract：:The genetic diversity between major meticillin-resistant Staphylococcus aureus (MRSA) lineages was probed using fluorescent amplified fragment length polymorphism (FAFLP) as a random genome sampling tool. Genomic DNA was digested with endonucleases BglII and Csp6I and a subset of the restricted fragments were amplifie...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.049429-0
更新日期：2012-12-01 00:00:00
abstract：:Bacteraemia in children is a potentially life-threatening condition. The objective of this study was to determine trends in the aetiology of bacteraemia in children aged 1 month-15 years in England and Wales by collecting data voluntarily reported by National Health Service hospital microbiology laboratories. Over the...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.015271-0
更新日期：2010-02-01 00:00:00
abstract：:We present a case of septicaemia caused by Arcanobacterium haemolyticum in a previously healthy 23-year-old man suffering from acute pharyngotonsillitis, who developed complicated Lemierre's syndrome. Three blood cultures (both aerobic and anaerobic) revealed the exclusive presence of A. haemolyticum. The presence of ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.007492-0
更新日期：2009-12-01 00:00:00
abstract：:Administration of oestradiol to three strains of female mice induced the oestrous phase of the reproductive cycle in which there are few or no polymorphonuclear leucocytes (PMNL) in vaginal smears. This treatment rendered the mice susceptible to genital tract colonisation by serotype 8 of Ureaplasma urealyticum, inocu...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-29-2-111
更新日期：1989-06-01 00:00:00
abstract：:There is a paucity of information about the factors associated with oral colonization with Candida species and the changes associated with antiretroviral (ARV) therapy. This study investigated the role of ARV therapy and other factors in a study population. Relevant clinical and laboratory information was obtained and...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.047522-0
更新日期：2013-01-01 00:00:00
abstract：:Most hospital bacteriologists have divided staphylococci into two groups: Staphylococcus aureus and the coagulase-negative staphylococci of which the novobiocin-resistant varieties are termed S. saprophyticus. The identification of S. aureus has been easy but that of the other staphylococci has provided some difficult...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-22-2-179
更新日期：1986-09-01 00:00:00
abstract：:The lipopolysaccharides (LPS) of the 10 species of the genus Bacteroides (sensu stricto) were extracted by the proteinase K method and their resolution compared by several methods of polyacrylamide gel electrophoresis (PAGE). These included sodium dodecyl sulphate (SDS)-PAGE with and without urea, polyacrylamide gradi...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-34-5-253
更新日期：1991-05-01 00:00:00
abstract：:Infections of the parotid gland with non-tuberculous mycobacteria (NTM) are rarely described. Here, we report on an infection of the parotid gland caused by Mycobacterium avium and give a literature-based overview about this entity. In the light of a global increase of mycobacterial infections, unusual manifestations ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.47698-0
更新日期：2008-04-01 00:00:00
abstract：:The effect of CO2 on the growth of 31 strains of Candida albicans was studied in serum and in a defined medium containing urea, ammonium chloride, asparagine, glutamine or acetamide as the nitrogen source. CO2 10% enhanced the mycelial growth of all strains when the medium contained an appropriate constituent to media...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-22-3-203
更新日期：1986-11-01 00:00:00
abstract：:A pathogenic vancomycin-resistant Staphylococcus aureus (VRSA) isolate (MIC > or =64 microg ml(-1)) was obtained from a Kolkata hospital in June 2005. Species identification was confirmed by Gram staining, standard biochemical tests and PCR amplification of the nuc gene, which encodes the thermostable nuclease that is...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.47144-0
更新日期：2008-01-01 00:00:00
abstract：:A serial study of rotavirus infection in gnotobiotic piglets is described. They were infected when 7 days old and the course of infection was followed for 21 days, by immunofluorescence and histological examinations of the intestinal epithelium and by titration of the virus content of the gut lumen. There appeared to ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-11-3-325
更新日期：1978-08-01 00:00:00
abstract：:A monospecific polyclonal antiserum, prepared against Bacteroides fragilis common polysaccharide antigen purified by polyacrylamide gel immunoblot detected B. fragilis, B. thetaiotaomicron, B. ovatus and Prevotella melaninogenica in pus samples from various anatomical sites by immunofluorescence microscopy of the pus....
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-43-2-99
更新日期：1995-08-01 00:00:00
abstract：:Francisella tularensis has been recognized as the causative agent of tularaemia for almost a century. Since its discovery in 1911, it has been shown to infect a wide range of hosts, including humans. As early as the 1920s it was suggested to be an intracellular pathogen, but it has proven to be an enigmatic organism, ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章,评审
doi：10.1099/jmm.0.2008/000653-0
更新日期：2008-08-01 00:00:00
abstract：:Members of the genus Actinomyces, Gram-positive, non-spore-forming anaerobic bacteria, are normal inhabitants of the mucosal surfaces of the oral, gastrointestinal and genital tracts. Identification of these bacteria using conventional methods is generally difficult because of their complex transport and growth requir...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.053165-0
更新日期：2013-05-01 00:00:00
abstract：:We have characterised 45 isolates of methicillin-resistant Staphylococcus aureus (MRSA) from Glasgow Royal Infirmary by means of simple biotyping, immunoblotting of exported proteins and restriction enzyme fragmentation patterns (REFP) of plasmid DNA. The strains were subdivided into four groups (A-D) on the basis of ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-31-2-125
更新日期：1990-02-01 00:00:00
abstract：:The heparin-binding properties of six different species of coagulase-negative staphylococci were examined by a particle agglutination assay. Heparin (mol. wt 4000-6000), mildly treated with sodium periodate, was covalently coupled to amino-modified latex beads (0.72 micron diameter). The particle agglutination assay w...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-45-4-263
更新日期：1996-10-01 00:00:00
abstract：:The release of interleukin-8 (IL-8) from human neutrophils was investigated after their internalisation of the invasive bacteria Listeria monocytogenes and Yersinia enterocolitica. Three pairs of bacterial strains were used to study the influence of listeriolysin O, invasiveness and Yersinia virulence plasmid (pYV)-en...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-47-1-55
更新日期：1998-01-01 00:00:00
abstract：PURPOSE:This study aimed to explore daptomycin combined with fosfomycin or rifampin against the planktonic and adherent linezolid-resistant isolates of Enterococcus faecalis. METHODOLOGY:Four linezolid-resistant and four linezolid-sensitive isolates of E. faecalis which formed biofilms were collected for this study. B...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000945
更新日期：2019-03-01 00:00:00
abstract：:Campylobacter jejuni is a frequent cause of infectious diarrhoea and is increasingly recognized as a trigger for late-onset complications. The poor standardization of commonly used serological tests might explain the conflicting results regarding the frequency of antecedent C. jejuni infections in defined patient grou...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.46040-0
更新日期：2005-08-01 00:00:00
abstract：:Five hundred clinical group A streptococcal (GAS) isolates were collected in Belgium during the period 1 Nov. 1993 to 31 Oct. 1994. Clinical and laboratory data were recorded and isolates were characterised. The presence of the genes encoding streptococcal pyrogenic exotoxin types A (speA), B (speB), C (speC), F (speF...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/0022-1317-49-5-467
更新日期：2000-05-01 00:00:00
abstract：:Leucocyte-egested material was harvested after the quantitative in-vitro phagocytosis of Neisseria meningitidis by rabbit or mouse polymorphonuclear leucocytes. The egested material was injected subcutaneously into rabbits and followed 24 h later with an intravenous injection of what would by itself have been a non-le...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-9-4-451
更新日期：1976-11-01 00:00:00
abstract：:Borderline oxacillin-resistant Staphylococcus aureus (BORSA) represents a quite poorly understood and inadequately defined phenotype of methicillin resistance. BORSA strains show low, borderline resistance to penicillinase-resistant penicillins (PRPs), with oxacillin MICs typically equal to 1-8 µg ml-1, and in contras...
journal_title：Journal of medical microbiology
pub_type： 杂志文章,评审
doi：10.1099/jmm.0.000585
更新日期：2017-10-01 00:00:00
abstract：:The purpose of this review is to discuss the scientific literature on waterborne healthcare-associated infections (HCAIs) published from 1990 to 2012. The review focuses on aquatic bacteria and describes both outbreaks and single cases in relation to patient characteristics, the settings and contaminated sources. An o...
journal_title：Journal of medical microbiology
pub_type： 杂志文章,评审
doi：10.1099/jmm.0.075713-0
更新日期：2014-10-01 00:00:00
abstract：:Providencia alcalifaciens is an invasive enteric pathogen. The present study determined the prevalence of invasive ability in P. alcalifaciens strains isolated in São Paulo, Brazil, mainly from patients with diarrhoea. Invasion of HeLa cells was found in 17 (42%) of 41 strains studied. Most (88%) of the invasive strai...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-45-6-459
更新日期：1996-12-01 00:00:00
abstract：:Interaction of human polymorphonuclear leucocytes (PMNL) and influenza virus (IFV) was studied in vitro. At 0 degree C, the viral particles were bound extensively to the surface of the PMNL membrane with a ratio of about 1000 virus particles to a single PMNL. The binding was sensitive to neuraminidase, suggesting atta...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-28-3-191
更新日期：1989-03-01 00:00:00
