Analysis of integrons and associated gene cassettes of metallo-β-lactamase-positive Pseudomonas aeruginosa in Malaysia.

Abstract:

:In this study, 90 non-replicate imipenem-resistant Pseudomonas aeruginosa (IRPA) Malaysian isolates collected between October 2005 and March 2008 were subjected to a screening test for detection of the integron and the gene cassette. Class 1 integrons were detected in 54 IRPA clinical isolates, whilst three isolates contained class 2 integrons. Analysis of the gene cassettes associated with the class 1 integrons showed the detection of accC1 in isolates carrying bla(IMP-7) and aacA7 in isolates carrying bla(VIM-2). aadA6 was detected in two isolates carrying bla(IMP-4). Using random amplification of polymorphic DNA analysis, 14 PCR fingerprint patterns were generated from the 32 isolates carrying metallo-β-lactamase (MBL) genes (35.5 %), whilst 20 patterns were generated from the 58 non-MBL gene isolates (64.4 %). Based on the differences in the fingerprinting patterns, two clusters (A and B) were identified among the MBL-producing isolates. Cluster A comprised 18 isolates (56 %) carrying the bla(VIM) gene, whereas cluster B comprised 14 (44 %) isolates carrying the bla(IMP) gene. The non-MBL isolates were divided into clusters C and D. Cluster C comprised 22 non-MBL isolates harbouring class 1 integrons, whilst cluster D consisted of three isolates carrying class 2 integrons. These findings suggest that the class 1 integron is widespread among P. aeruginosa isolated in Malaysia and that characterization of cassette arrays of integrons will be a useful epidemiological tool to study the evolution of multidrug resistance and the dissemination of antibiotic resistance genes.

journal_name

J Med Microbiol

authors

Khosravi Y,Tay ST,Vadivelu J

doi

10.1099/jmm.0.029868-0

subject

Has Abstract

pub_date

2011-07-01 00:00:00

pages

988-994

issue

Pt 7

eissn

0022-2615

issn

1473-5644

journal_volume

60

pub_type

杂志文章
  • Ovine systemic pasteurellosis caused by Pasteurella haemolytica biotype T.

    abstract::A detailed study was made of lambs aged 5--7 months naturally infected with Pasteurella haemolytica biotype T. In addition to the well known features of such infections, previously unreported necrotic lesions of the tonsil, oesophagus, pharynx and adjacent areas were consistently seen. Large numbers of P. haemolytica ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-14-1-89

    authors: Dyson DA,Gilmour NJ,Angus KW

    更新日期:1981-02-01 00:00:00

  • Age-related changes in the prevalence of precipitating antibodies to BK virus in infants and children.

    abstract::Nearly 1000 sera from children were tested by immunoelectro-osmophoresis against BK virus, and age-specific prevalence rates were estimated from birth until the age of 12 years. Declining rates during the first 12 months showed the waning of passive immunity, which at birth reflects the mother's immune status. The cha...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-15-3-285

    authors: Dei R,Marmo F,Corte D,Sampietro MG,Franceschini E,Urbano P

    更新日期:1982-08-01 00:00:00

  • Dose escalation studies with caspofungin against Candida glabrata.

    abstract::Echinocandins are recommended as first-line agents against invasive fungal infections caused by Candida glabrata, which still carry a high mortality rate. Dose escalation of echinocandins has been suggested to improve the clinical outcome against C. glabrata. To address this possibility, we performed in vitro and in v...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000116

    authors: Domán M,Kovács R,Perlin DS,Kardos G,Gesztelyi R,Juhász B,Bozó A,Majoros L

    更新日期:2015-09-01 00:00:00

  • Effect of carbon dioxide on the growth and form of Candida albicans.

    abstract::The effect of CO2 on the growth of 31 strains of Candida albicans was studied in serum and in a defined medium containing urea, ammonium chloride, asparagine, glutamine or acetamide as the nitrogen source. CO2 10% enhanced the mycelial growth of all strains when the medium contained an appropriate constituent to media...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-22-3-203

    authors: Sims W

    更新日期:1986-11-01 00:00:00

  • Evaluation of antigenic variations between two virulent toxoplasma strains.

    abstract::Toxoplasma gondii infection in humans is routinely assessed by serological means. Here, the authors attempted to compare the response of different Toxoplasma strains to serological tests and to evaluate the antigenic profiles of the RH and RH Ankara (TRH) strains with Western blotting. Anti-Toxoplasma IgG antibodies o...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46712-0

    authors: Delibas SB,Ertabaklar H,Ertug S

    更新日期:2006-10-01 00:00:00

  • Differential adhesion and invasion by Staphylococcus aureus of epithelial cells derived from different anatomical sites.

    abstract::Staphylococcus aureus can invade epithelial cells, and the host-cell receptor α(5)β(1) integrin is thought to mediate this process. The aim of this study was to investigate S. aureus invasion of epithelial cell lines derived from oral (H357), skin (UP) and nasopharyngeal (Detroit 562) sites and to determine whether an...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.049650-0

    authors: Ridley RA,Douglas I,Whawell SA

    更新日期:2012-12-01 00:00:00

  • Antimicrobial resistance in the nasopharyngeal flora of children with acute otitis media and otitis media recurring after amoxicillin therapy.

    abstract::The objective of this study was to investigate the antimicrobial susceptibility of the organisms isolated from the nasopharynx of children who presented with acute otitis media (AOM) or otitis media that recurred after amoxicillin therapy. Nasopharyngeal cultures obtained from 72 patients, 40 with AOM and 32 with recu...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45819-0

    authors: Brook I,Gober AE

    更新日期:2005-01-01 00:00:00

  • Pyrolysis mass spectrometry in epidemiological and population genetic studies of Haemophilus influenzae.

    abstract::Haemophilus influenzae serotype b (Hib) vaccines have reduced the amount of invasive Hib disease in immunised infants. However, Hib disease remains in unvaccinated infants and adults and non-capsulate H. influenzae (NCHi) still causes infections, including outbreaks of respiratory disease. Characterisation of strains ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-46-3-204

    authors: Leaves NI,Sisson PR,Freeman R,Jordens JZ

    更新日期:1997-03-01 00:00:00

  • Post-operative endophthalmitis due to an unusual pathogen, Comamonas testosteroni.

    abstract::Here, we describe the first report of post-operative endophthalmitis due to Comamonas testosteroni in an elderly diabetic patient after complicated cataract surgery. The isolate was identified by using Mini API strips. The patient was successfully treated with intravitreal ceftazidime and oral ciprofloxacin. ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.006072-0

    authors: Reddy AK,Murthy SI,Jalali S,Gopinathan U

    更新日期:2009-03-01 00:00:00

  • Gas-chromatographic lipid profiles in identification of currently known slowly growing environmental mycobacteria.

    abstract::Cellular fatty acid analysis by GLC is widely used in the species identification of mycobacteria. Combining mycolic acid cleavage products with shorter cellular fatty acids increases the informative value of the analysis. A key has been created to aid in the identification of all currently known slowly growing environ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.05113-0

    authors: Torkko P,Katila ML,Kontro M

    更新日期:2003-04-01 00:00:00

  • Isolation of haemin-binding proteins of Neisseria gonorrhoeae.

    abstract::Although Neisseria gonorrhoeae can use haem as the sole exogenous iron source for growth in vitro, the mechanism of haem-iron uptake in the gonococcus is unknown. Two haemin-binding proteins (HmBPs) of 97 and 44 Kda were isolated by batch ligand affinity-chromatography from whole cells or total membranes of gonococci ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-36-2-121

    authors: Lee BC

    更新日期:1992-02-01 00:00:00

  • Identification of oral bacteria associated with crevicular epithelial cells from chronic periodontitis lesions.

    abstract::Bacterial invasion of host epithelial cells plays an important role in the pathogenesis of periodontal diseases; however, the interactions between subgingival species and the gingival crevice cells are not fully understood. This study determined the prevalence of a group of oral bacterial species on or in epithelial c...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46417-0

    authors: Colombo AV,Silva CM,Haffajee A,Colombo APV

    更新日期:2006-05-01 00:00:00

  • Influence of various immunosuppressive agents on the occurrence of endogenous bacteraemia in mice.

    abstract::The influence of six immunosuppressive agents on the occurrence of endogenous bacteraemia in mice was evaluated. The mortality rates in conventional ddY mice given cyclophosphamide (CY), fluorouracil (5-FU), methotrexate (MTX), cisplatin (CDDP) or FK-506 intraperitoneally, or dexamethasone (DXM) subcutaneously were 70...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-42-3-181

    authors: Hirakata Y,Furuya N,Matsumoto T,Tateda K,Yamaguchi K

    更新日期:1995-03-01 00:00:00

  • Prevalence of invasive ability and other virulence-associated characteristics in Providencia alcalifaciens strains isolated in São Paulo, Brazil.

    abstract::Providencia alcalifaciens is an invasive enteric pathogen. The present study determined the prevalence of invasive ability in P. alcalifaciens strains isolated in São Paulo, Brazil, mainly from patients with diarrhoea. Invasion of HeLa cells was found in 17 (42%) of 41 strains studied. Most (88%) of the invasive strai...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-45-6-459

    authors: Guth BE,Perrella E

    更新日期:1996-12-01 00:00:00

  • Characteristics of a pandemic clone of O3 : K6 and O4 : K68 Vibrio parahaemolyticus isolated in Beira, Mozambique.

    abstract::The genetic characteristics of Vibrio parahaemolyticus strains isolated in 2004 and 2005 in Mozambique were assessed in this study to determine whether the pandemic clone of V. parahaemolyticus O3 : K6 and O4 : K68 serotypes has spread to Mozambique. Fifty-eight V. parahaemolyticus strains isolated from hospitalized d...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.2008/004275-0

    authors: Ansaruzzaman M,Chowdhury A,Bhuiyan NA,Sultana M,Safa A,Lucas M,von Seidlein L,Barreto A,Chaignat CL,Sack DA,Clemens JD,Nair GB,Choi SY,Jeon YS,Lee JH,Lee HR,Chun J,Kim DW

    更新日期:2008-12-01 00:00:00

  • Inhibition of quorum-sensing-controlled virulence factors of Pseudomonas aeruginosa by Murraya koenigii essential oil: a study in a Caenorhabditis elegans infectious model.

    abstract::The global emergence of antibiotic-resistant strains of Pseudomonas aeruginosa poses a major threat in both hospital environments and the community. P. aeruginosa is an opportunistic human pathogen, and it also infects a wide range of model organisms including the nematode Caenorhabditis elegans. Quorum sensing (QS) m...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000385

    authors: Ganesh PS,Rai RV

    更新日期:2016-12-01 00:00:00

  • Comparative growth of Bacteroides species in various anaerobic culture media.

    abstract::The growth of five species of Bacteroides in four anaerobic culture media was continuously monitored turbidimetrically. Interspecies differences were observed in the growth of Bacteroides spp. in the various media, but growth in Brain Heart Infusion broth supplemented with yeast extract, haemin and menadione, was cons...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-19-2-195

    authors: Eley A,Greenwood D,O'Grady F

    更新日期:1985-04-01 00:00:00

  • Do English NHS Microbiology laboratories offer adequate services for the diagnosis of UTI in children? Healthcare Quality Improvement Partnership (HQIP) Audit of Standard Operational Procedures.

    abstract::The National Institute of Care Excellence (NICE) 2007 guidance CG54, on urinary tract infection (UTI) in children, states that clinicians should use urgent microscopy and culture as the preferred method for diagnosing UTI in the hospital setting for severe illness in children under 3 years old and from the GP setting ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000114

    authors: McNulty CAM,Verlander NQ,Moore PCL,Larcombe J,Dudley J,Banerjee J,Jadresic L

    更新日期:2015-09-01 00:00:00

  • Utility of real-time amplification of selected 16S rRNA gene sequences as a tool for detection and identification of microbial signatures directly from clinical samples.

    abstract::The potential of incorporating a real-time PCR for amplification and detection of 16S rRNA gene signatures directly from clinical samples was assessed as a tool for diagnostics. Universal PCR primers spanning short variable regions (~500 bp) were optimized for real-time PCR and tested in comparison with a longer fragm...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.041764-0

    authors: Edwards KJ,Logan JMJ,Langham S,Swift C,Gharbia SE

    更新日期:2012-05-01 00:00:00

  • Role of fibronectin-binding protein A in Clostridium difficile intestinal colonization.

    abstract::Clostridium difficile is a frequent cause of severe, recurrent, post-antibiotic diarrhoea and pseudomembranous colitis. Its pathogenicity is mediated mainly by two toxins, TcdA and TcdB. However, different adhesins have also been described as important colonization factors which are implicated in the first step of the...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.029553-0

    authors: Barketi-Klai A,Hoys S,Lambert-Bordes S,Collignon A,Kansau I

    更新日期:2011-08-01 00:00:00

  • Widespread clone of Burkholderia cenocepacia in cystic fibrosis patients in the Czech Republic.

    abstract::The morbidity and mortality rates in patients with cystic fibrosis (CF) are significantly affected by infections with Burkholderia cepacia complex. In a Czech CF Centre, the prevalence of the infection reached up to 30 %, with the majority of patients found to be infected with Burkholderia cenocepacia (formerly genomo...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46025-0

    authors: Drevinek P,Vosahlikova S,Cinek O,Vavrova V,Bartosova J,Pohunek P,Mahenthiralingam E

    更新日期:2005-07-01 00:00:00

  • Inhibition of Helicobacter pylori infection by bovine milk glycoconjugates in a BAlb/cA mouse model.

    abstract::The attachment of Helicobacter pylori to the human gastric mucosa is a complex process involving several specific structures recognised by the cell surface receptors. Sialylated multivalent high mol. wt glycoproteins have been shown to inhibit H. pylori sialic acid-specific haemagglutination. This study explored wheth...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-50-5-430

    authors: Wang X,Hirmo S,Willén R,Wadström T

    更新日期:2001-05-01 00:00:00

  • Visceral leishmaniasis/human immunodeficiency virus co-infection in India: the focus of two epidemics.

    abstract::India contributes heavily to the global burden of visceral leishmaniasis (VL, kala-azar) and human immunodeficiency virus (HIV)/AIDS. The prevalence of HIV seropositivity in VL patients at a tertiary care centre in northern India, as observed during a prospective study over a period of 2 years, is presented. Of the 10...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46574-0

    authors: Mathur P,Samantaray JC,Vajpayee M,Samanta P

    更新日期:2006-07-01 00:00:00

  • Escherichia coli antibodies in opsonisation and protection against infection.

    abstract::The opsonic and protective capacities of rabbit antisera against Escherichia coli O, K and core-glycolipid cell-wall antigens were compared with specific antibody titres as measured by agglutination and enzyme-linked immunosorbent assay. Anti-O antisera were opsonic and protective against two noncapsulate strains. Onl...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-14-4-381

    authors: van Dijk WC,Verbrugh HA,van Erne-van der Tol ME,Peters R,Verhoef J

    更新日期:1981-11-01 00:00:00

  • Antibodies to Shiga toxin in the serum of children with Shigella-associated haemolytic uraemic syndrome.

    abstract::Antibodies to Shiga toxin (Stx) were measured in the sera of 49 children with Shigella dysenteriae serotype 1 infection, of whom 17 had haemolytic uraemic syndrome (HUS) and 32 had no complications (uncomplicated shigellosis, UCS). Children with HUS had lower levels of total IgG and IgM and lower IgM titres to Stx tha...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-48-1-11

    authors: Azim T,Rashid A,Qadri F,Sarker MS,Hamadani J,Salam MA,Wahed MA,Albert MJ

    更新日期:1999-01-01 00:00:00

  • Production of extracellular matrix by Candida albicans biofilms.

    abstract::Growth of Candida albicans biofilms and production of extracellular matrix were monitored by dry weight, colorimetric and radioisotope assays, and by scanning electron microscopy. Under static incubation conditions synthesis of matrix material was minimal, but increased dramatically when developing biofilms were subje...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-47-3-253

    authors: Hawser SP,Baillie GS,Douglas LJ

    更新日期:1998-03-01 00:00:00

  • DNA identification of the pathogen of candidal aspiration pneumonia induced in the course of oral cancer therapy.

    abstract::Aspiration of oropharyngeal bacteria and fungi is occasionally suspected in patients with pneumonia. A patient with oral carcinoma underwent chemoradioimmunotherapy and, about 4 weeks from the start of the therapy, the patient suffered from severe oral mucositis induced by chemoradiotherapy, and candidal pneumonia was...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45769-0

    authors: Yamamoto T,Ueta E,Kamatani T,Osaki T

    更新日期:2005-05-01 00:00:00

  • In vitro efficacy of sodium selenite in reducing toxin production, spore outgrowth and antibiotic resistance in hypervirulent Clostridium difficile.

    abstract:PURPOSE:This study investigated the efficacy of the essential mineral, selenium (sodium selenite), in reducing the toxin production, spore outgrowth and antibiotic resistance of Clostridium difficile in vitro. METHODOLOGY:Two hypervirulent C. difficile isolates were cultured in brain heart infusion broth with and with...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001008

    authors: Pellissery AJ,Vinayamohan PG,Yin HB,Mooyottu S,Venkitanarayanan K

    更新日期:2019-07-01 00:00:00

  • Leclercia adecarboxylata in an immunocompetent patient.

    abstract::Leclercia adecarboxylata is a rarely reported human pathogen, most commonly affecting immunocompromised individuals. In reported cases of immunocompetent patients infected with this organism, it is seen exclusively in the context of polymicrobial infections. We report here the case of an abscess in an immunocompetent ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.47673-0

    authors: Hess B,Burchett A,Huntington MK

    更新日期:2008-07-01 00:00:00

  • Restoring the activity of the antibiotic aztreonam using the polyphenol epigallocatechin gallate (EGCG) against multidrug-resistant clinical isolates of Pseudomonas aeruginosa.

    abstract::Introduction. Pseudomonas aeruginosa is an important Gram-negative pathogen that is intrinsically multidrug-resistant (MDR) and frequently associated with healthcare-associated outbreaks. With increasing resistance to antibiotics and with very few novel drugs under development, clinicians often use combinations to tre...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001060

    authors: Betts JW,Hornsey M,Higgins PG,Lucassen K,Wille J,Salguero FJ,Seifert H,La Ragione RM

    更新日期:2019-10-01 00:00:00