Comparison of two automated instruments for Epstein-Barr virus serology in a large adult hospital and implementation of an Epstein-Barr virus nuclear antigen-based testing algorithm.

abstract：:This is the first report, to our knowledge, of two temporally and geographically related nosocomial Asaia lannensis infections in a paediatric setting. Two patients with idiopathic dilated cardiomyopathy awaiting cardiac transplantation developed bacteraemia during their hospital stay. The physical location of both pa...

journal_title：Journal of medical microbiology

authors： Juretschko S,Beavers-May TK,Stovall SH

更新日期：2010-07-01 00:00:00

abstract：:Mycoplasma fermentans has attracted much interest both as a cofactor for the progression of AIDS and as a pathogenic agent in non-AIDS related diseases. Previous studies with serological and genetic techniques suggest that M. fermentans represents a homogeneous group of organisms, with no significant differences ident...

journal_title：Journal of medical microbiology

authors： Hannan PC,Kearns AM,Sisson PR,Freeman R

更新日期：1997-04-01 00:00:00

abstract：:Aggregatibacter actinomycetemcomitans is commonly part of the normal microflora of the human upper respiratory tract. It has been implicated in periodontal disease and various infections, particularly endocarditis. We report here what we believe to be the first case of recurrent infective endocarditis due to A. actino...

journal_title：Journal of medical microbiology

authors： Potron A,Mainardi JL,Podglajen I,Meunier F,Sanson-le Pors MJ,Berçot B

更新日期：2010-12-01 00:00:00

abstract：:This study investigated the comparative performance of the Amplicor assay and an in-house semi-automated, multiplex real-time PCR for the diagnosis of genital chlamydial infection. Four different assays, the COBAS Amplicor CT test (Amplicor PCR), in-house real-time PCR (IHRT-PCR), in-house nested cryptic plasmid PCR a...

journal_title：Journal of medical microbiology

authors： Jalal H,Al-Suwaine A,Stephen H,Carne C,Sonnex C

更新日期：2007-03-01 00:00:00

abstract：:A rapid and simple method for counting viable bacteria is described. The technique involves the use of a semi-automatic micropipette and disposable glass-capillary tubes. Viable counts obtained by this method were not significantly different from those obtained by the Miles, Misra and Irwin method or by a pour-plate m...

journal_title：Journal of medical microbiology

authors： Slack MP,Wheldon DB

更新日期：1978-11-01 00:00:00

abstract：:Gardnerella vaginalis is an important component of the human vaginal microflora. It is proposed to play a key role in the pathogenesis of bacterial vaginosis (BV), the most common vaginal condition. Here we describe the development, validation and comparative analysis of a novel molecular approach capable of G. vagina...

journal_title：Journal of medical microbiology

authors： Balashov SV,Mordechai E,Adelson ME,Gygax SE

更新日期：2014-02-01 00:00:00

abstract：:Mice were vaccinated with lipopolysaccharide (LPS) from Leptospira interrogans serovar pomona or hardjo, or with the polysaccharide (PS) fraction of the LPS, or with an immunoconjugate of PS and diphtheria toxoid (DT). Maximum agglutinin titres were found 6-10 weeks after vaccination with LPS or PS-DT conjugate; the l...

journal_title：Journal of medical microbiology

authors： Midwinter A,Faine S,Adler B

更新日期：1990-11-01 00:00:00

abstract：:Biofilms are colonies of microbial cells encased in a self-produced organic polymeric matrix and represent a common mode of microbial growth. Microbes growing as biofilm are highly resistant to commonly used antimicrobial drugs. We aimed to screen and characterize biofilm formation by different isolates of Candida on ...

journal_title：Journal of medical microbiology

authors： Zahran KM,Agban MN,Ahmed SH,Hassan EA,Sabet MA

更新日期：2015-04-01 00:00:00

abstract：:Shigella sonnei infection in industrialized countries is often associated with foreign travel. A total of 195 S. sonnei isolates in Japan, isolated from cases associated with foreign travel, were analysed by biotyping and molecular typing using PFGE and multilocus variable-number tandem-repeat analysis (MLVA); their a...

journal_title：Journal of medical microbiology

authors： Izumiya H,Tada Y,Ito K,Morita-Ishihara T,Ohnishi M,Terajima J,Watanabe H

更新日期：2009-11-01 00:00:00

abstract：:Experiments were performed to determine whether a modern flow cytometer could be used to study bacterial populations in suspension, with particular reference to their morphological characteristics and their responses to antibiotics. The FACScan, a commercial benchtop flow cytometer fitted with an air-cooled laser, des...

journal_title：Journal of medical microbiology

authors： Gant VA,Warnes G,Phillips I,Savidge GF

更新日期：1993-08-01 00:00:00

abstract：:The lipopolysaccharides (LPS) of the 10 species of the genus Bacteroides (sensu stricto) were extracted by the proteinase K method and their resolution compared by several methods of polyacrylamide gel electrophoresis (PAGE). These included sodium dodecyl sulphate (SDS)-PAGE with and without urea, polyacrylamide gradi...

journal_title：Journal of medical microbiology

authors： Maskell JP

更新日期：1991-05-01 00:00:00

abstract：:Streptococcus gordonii produces a pheromone heptapeptide, s.g.cAM373, which induces a conjugative mating response in Enterococcus faecalis cells carrying the responsive plasmid, pAM373. We investigated the extent of this intergeneric signaling on DNA acquisition by streptococcal species likely to cohabit oral biofilms...

journal_title：Journal of medical microbiology

authors： Mansfield JM,Herrmann P,Jesionowski AM,Vickerman MM

更新日期：2017-11-01 00:00:00

abstract：:Carbapenems are first-line agents for the treatment of serious nosocomial infections caused by multidrug-resistant Enterobacteriaceae. However, resistance to carbapenems has increased dramatically among Enterobacteriaceae in our hospital. In this study, we report clonal dissemination caused by carbapenem-resistant Ent...

journal_title：Journal of medical microbiology

authors： Qin X,Yang Y,Hu F,Zhu D

更新日期：2014-02-01 00:00:00

abstract：:Some strains of Helicobacter pylori are known to produce an extracellular cytotoxin that causes vacuolation in cultured mammalian cells. Screening for such strains makes use of HeLa cells which may not be sensitive enough to detect minimal changes. The aim of this study was to develop a more sensitive cell line. Vacuo...

journal_title：Journal of medical microbiology

authors： Wang XM,Kojima T,Satoh K,Taniguchi Y,Tokumaru K,Saifuku K,Seki M,Kihira K,Ido K,Uchida JY,Ohmori C,Takaoka T,Kimura K

更新日期：1997-08-01 00:00:00

abstract：:This study used a previously described multiplex PCR-based reverse line blot (mPCR/RLB) assay to assess the prevalence and distribution of 14 urogenital pathogens or putative pathogens, namely Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma genitalium, Mycoplasma hominis, Trichomonas vaginalis, Gardnerella va...

journal_title：Journal of medical microbiology

authors： McKechnie ML,Hillman RJ,Jones R,Lowe PC,Couldwell DL,Davies SC,King F,Kong F,Gilbert GL

更新日期：2011-07-01 00:00:00

abstract：:Large-scale monitoring of resistance to 14 antimicrobial agents was performed using 103 Proteus mirabilis strains isolated from dogs in Japan. Resistant strains were analysed to identify their resistance mechanisms. Rates of resistance to chloramphenicol, streptomycin, enrofloxacin, trimethoprim/sulfamethoxazole, kana...

journal_title：Journal of medical microbiology

authors： Harada K,Niina A,Shimizu T,Mukai Y,Kuwajima K,Miyamoto T,Kataoka Y

更新日期：2014-11-01 00:00:00

abstract：:Pseudomonas aeruginosa is an opportunistic human pathogen that causes serious and chronic infections. Many secondary metabolites are secreted throughout its growth, among which phenazine is a known virulence factor and signalling molecule. Phenazine is coordinately controlled by the global regulatory quorum-sensing (Q...

journal_title：Journal of medical microbiology

authors： Liang H,Duan J,Sibley CD,Surette MG,Duan K

更新日期：2011-01-01 00:00:00

abstract：:Cell-surface hydrophobicity of Helicobacter (formerly Campylobacter) pylori was tested by aqueous two-phase partitioning and hydrophobic interaction chromatography. The hydrophobicity of H. pylori greatly exceeded that of Campylobacter fetus subsp. fetus, C. jejuni and Bacillus subtilis. A partition coefficient (PC) o...

journal_title：Journal of medical microbiology

authors： Pruul H,Goodwin CS,McDonald PJ,Lewis G,Pankhurst D

更新日期：1990-06-01 00:00:00

abstract：:A slide agglutination technique identifying 10 serogroups of Clostridium difficile (A,B,C,D,F,G,H,I,K and X) has been described previously. In this study, we have used the hamster to compare the ability of the 10 serogroup reference strains to colonise and produce disease. Groups of four hamsters were each given a sin...

journal_title：Journal of medical microbiology

authors： Delmée M,Avesani V

更新日期：1990-10-01 00:00:00

abstract：PURPOSE:Persister cells, a subpopulation of tolerant cells within the bacterial culture, are commonly thought to be responsible for antibiotic therapy failure and infection recurrence. Klebsiella pneumoniae is a notorious human pathogen for its increasing resistance to antibiotics and wide involvement in severe infecti...

journal_title：Journal of medical microbiology

authors： Li Y,Zhang L,Zhou Y,Zhang Z,Zhang X

更新日期：2018-03-01 00:00:00

abstract：:Introduction. Infections with the respiratory pathogen Mycoplasma pneumoniae are often chronic, recurrent and resistant, persisting after antibiotic treatment. M. pneumoniae grown on glass forms protective biofilms, consistent with a role for biofilms in persistence. These biofilms consist of towers of bacteria inters...

journal_title：Journal of medical microbiology

authors： Feng M,Burgess AC,Cuellar RR,Schwab NR,Balish MF

更新日期：2021-01-01 00:00:00

abstract：:The potential of human plasma (HP) or serum (HS) as a replacement for fetal calf serum (FCS) was evaluated in a liver infusion tryptose (LIT) medium for bulk cultivation of Leishmania donovani promastigotes. The promastigote yield with the LIT-FCS standard medium was 0.4-1.8×10(7) ml(-1), and yields of 0.5-3.4×10(7) (...

journal_title：Journal of medical microbiology

authors： Mahamoud A,Osman HA,Mansour D,El Harith A

更新日期：2013-08-01 00:00:00

abstract：:Rapid detection of micro-organisms from blood is one of the most critical functions of a diagnostic microbiology laboratory. Automated blood-culture systems reduce the time needed to detect positive cultures, and reduce specimen handling. The false-positive rate of such systems is 1-10%. In this study, the presence of...

journal_title：Journal of medical microbiology

authors： Karahan ZC,Mumcuoglu I,Guriz H,Tamer D,Balaban N,Aysev D,Akar N

更新日期：2006-01-01 00:00:00

abstract：:Mucosal immunity in the gastrointestinal (GI) tract is a primary defence against GI pathogens. We hypothesise that a mucosal response to lipopolysaccharide (LPS), especially to the common (core) determinants of GI pathogenic Escherichia coli strains, is protective. The aims of this study were to investigate the specif...

journal_title：Journal of medical microbiology

authors： Currie CG,McCALLUM K,Poxton IR

更新日期：2001-04-01 00:00:00

abstract：:Circulating immune complexes (ICs) containing IgG and HBsAg, and IgG and HBeAg, in sera from groups of patients with various liver diseases were sought by ELISA and immunodiffusion. A correlation was found between the absence of ICs and the disappearance of HBsAg in patients who had recovered from acute hepatitis B, b...

journal_title：Journal of medical microbiology

authors： Leyva A,Bernal MC,Piedrola G,Maroto MC

更新日期：1988-07-01 00:00:00

abstract：:The aim of this retrospective study was to determine the clinical spectrum of group B streptococcal (GBS) bacteraemia in patients over 70 years old. Sixty-six adults with GBS bacteraemia were reviewed over a 5-year period. Disease characteristics, clinical diagnoses and underlying disease were compared in 33 older pat...

journal_title：Journal of medical microbiology

authors： Trivalle C,Martin E,Martel P,Jacque B,Menard JF,Lemeland JF

更新日期：1998-07-01 00:00:00

abstract：:Neisseria sicca is rarely associated with clinical infections and to the authors' knowledge this organism has not been reported as a causative agent of infected biloma (liver abscess). A case of a diabetic man with infected biloma due to N. sicca after repeated transcatheter arterial embolization for hepatocellular ca...

journal_title：Journal of medical microbiology

authors： Chung HC,Teng LJ,Hsueh PR

更新日期：2007-11-01 00:00:00

abstract：:Chronic meningococcaemia is an unusual clinical presentation of Neisseria meningitidis infection. We describe the case of a patient, who presented with total IgA deficiency and partial IgM deficiency with a low switched memory B cells count, suggestive of a borderline form of common variable immunodeficiency (CVID). T...

journal_title：Journal of medical microbiology

authors： Theulin A,Rondeau-Lutz M,Kuhnert C,Boileau J,Weber JC

更新日期：2010-11-01 00:00:00

abstract：:An affinity procedure with purified, biotinylated human transferrin and streptavidin-agarose was used to identify the transferrin-binding proteins in strains of Haemophilus influenzae. Proteins of 58 and 98 Kda were isolated from total membranes prepared from iron-deficient but not iron-sufficient H. influenzae KC548 ...

journal_title：Journal of medical microbiology

authors： Schryvers AB

更新日期：1989-06-01 00:00:00

abstract：:Seven isolates of B. piliformis, the agent of Tyzzer's disease, obtained from various host species, were examined for cytotoxic activity by incubating culture filtrates on BRL 3A rat-hepatocyte and 3T3 mouse-fibroblast cell lines. One isolate exhibited cytopathic effects on BRL 3A cells, but not on 3T3 cells. Three ot...

journal_title：Journal of medical microbiology

authors： Riley LK,Caffrey CJ,Musille VS,Meyer JK

更新日期：1992-07-01 00:00:00