Abstract:
:A rapid and simple method for counting viable bacteria is described. The technique involves the use of a semi-automatic micropipette and disposable glass-capillary tubes. Viable counts obtained by this method were not significantly different from those obtained by the Miles, Misra and Irwin method or by a pour-plate method. The micropipette procedure is less hazardous than techniques involving the use of calibrated dropping pipettes.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Slack MP,Wheldon DBdoi
10.1099/00222615-11-4-541subject
Has Abstractpub_date
1978-11-01 00:00:00pages
541-5issue
4eissn
0022-2615issn
1473-5644journal_volume
11pub_type
杂志文章abstract:PURPOSE:Pertussis remains a public health concern in most countries. Our study aimed to prospectively explore the epidemiology of pertussis in the Tunis area of Tunisia between 2007 and 2016, and to characterize the virulence-associated genes of the collected Bordetella pertussis isolates. METHODOLOGY:Infants and chil...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000892
更新日期:2019-02-01 00:00:00
abstract::A longitudinal analysis was carried out of the colonization by four potential respiratory pathogens - Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis and Staphylococcus aureus - in 165 healthy children (aged 3-7 years) attending three kindergartens and 417 healthy children (aged 7-10 years) att...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.040584-0
更新日期:2012-05-01 00:00:00
abstract::The resistance of Balamuthia mandrillaris to physical, chemical and radiological conditions was tested. Following treatments, viability was determined by culturing amoebae on human brain microvascular endothelial cells for up to 12 days. B. mandrillaris cysts were resistant to repeated freeze-thawing (five times), tem...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.47694-0
更新日期:2008-04-01 00:00:00
abstract::Two types of fimbriae, designated aggregative adherence fimbria I and II (AAF/I and AAF/II) have been described in enteroaggregative Escherichia coli (EAEC) strains. These fimbriae mediate the aggregative pattern of adherence (AA) to epithelial cells. The genes encoding the structural subunit of each fimbria have been...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-48-6-597
更新日期:1999-06-01 00:00:00
abstract::The family Adenoviridae consists of five genera of which the genus Mastadenovirus includes human viruses classified into 57 serotypes clustered into seven subgroups (A-G). Serotypes 40 and 41 (subgroup F) are specifically associated with childhood gastroenteritis and are the most common cause of acute gastroenteritis ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.072413-0
更新日期:2014-06-01 00:00:00
abstract::Erysipelothrix rhusiopathiae is a zoonotic pathogen that causes erysipelas in a variety of animals. In humans, in contrast to the cutaneous form called erysipeloid, which is an occupational disease and common in individuals who handle raw meat and fish, invasive systemic infections are unusual. E. rhusiopathiae expres...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.001252
更新日期:2020-10-01 00:00:00
abstract::A case of Salmonella typhimurium pericarditis is reported. The diagnosis was based on blood and pericardial effusion cultures. ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.05449-0
更新日期:2004-10-01 00:00:00
abstract::The substrates inositol, rhamnose, d-tartrate and m-tartrate used in fermentation tests with 338 cultures of Salmonella paratyphi B differentiated strains in some phage types to give information that could be used in epidemiological investigations. Xylose in Bitter's medium, the fifth substrate by which 13 of a potent...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-26-4-285
更新日期:1988-08-01 00:00:00
abstract::To clarify further the role of chemotaxis in Helicobacter pylori colonization, the in vitro bacterium response to human plasma and bile (secretions containing chemoeffector compounds that are present in the gastric mucus layer) was examined. Human plasma, after dilution to 1 % (v/v) with buffer, was found to be a chem...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.45636-0
更新日期:2004-08-01 00:00:00
abstract:INTRODUCTION:Idiopathic pulmonary fibrosis (IPF) is a chronic and ultimately fatal lung disease. One of the risk factors involved in the acquisition of IPF is viral infections, especially respiratory viruses. In the present study, we investigated the detection of respiratory viruses and the possible relationship betwee...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000628
更新日期:2017-11-01 00:00:00
abstract::Aspiration of oropharyngeal bacteria and fungi is occasionally suspected in patients with pneumonia. A patient with oral carcinoma underwent chemoradioimmunotherapy and, about 4 weeks from the start of the therapy, the patient suffered from severe oral mucositis induced by chemoradiotherapy, and candidal pneumonia was...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.45769-0
更新日期:2005-05-01 00:00:00
abstract::Here, we describe the first report of post-operative endophthalmitis due to Comamonas testosteroni in an elderly diabetic patient after complicated cataract surgery. The isolate was identified by using Mini API strips. The patient was successfully treated with intravitreal ceftazidime and oral ciprofloxacin. ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.006072-0
更新日期:2009-03-01 00:00:00
abstract::Molecular typing is an important tool for control and prevention of infection. A suitable molecular typing method for epidemiological investigation must be easy to perform, highly reproducible, inexpensive, rapid and easy to interpret. In this study, two molecular typing methods including the conventional PCR-sequenci...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000574
更新日期:2017-09-01 00:00:00
abstract:PURPOSE:Despite hundreds of reports on the isolation of Nocardia from clinical samples, the presence and diversity of Nocardia species that are capable of survival in a harsh and adverse condition, such as a hospital environment, have not been comprehensively studied. The aim of this study was to assess Nocardia specie...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000436
更新日期:2017-03-01 00:00:00
abstract:PURPOSE:This study investigated the efficacy of the essential mineral, selenium (sodium selenite), in reducing the toxin production, spore outgrowth and antibiotic resistance of Clostridium difficile in vitro. METHODOLOGY:Two hypervirulent C. difficile isolates were cultured in brain heart infusion broth with and with...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.001008
更新日期:2019-07-01 00:00:00
abstract::A series of 178 strains of Escherichia coli, highly resistant to trimethoprim, isolated from hospital patients and patients in the community between 1979 and 1983, was examined for the presence of Tn7 on a plasmid or on the chromosome only, by transposition to RP4 and restriction endonuclease digestion with Hind III. ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-22-2-125
更新日期:1986-09-01 00:00:00
abstract:PURPOSE:The analytical performance of the cobas 6800 HIV-1, HBV and HCV assays was verified and evaluated to the COBAS Ampliprep/COBAS TaqMan assays. METHODOLOGY:The precision, limit of detection (LoD), limit of quantification (LoQ) and linearity were verified using pooled residual clinical samples. The analytical spe...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000838
更新日期:2018-12-01 00:00:00
abstract::Introduction. The worldwide emergence of carbapenem resistance in Gram-negative bacteria makes the development of simple tests mandatory to identify antimicrobial resistance mechanisms. Enzymatic and membrane barriers are the prominent resistance mechanisms described in these bacteria. Several tests are currently used...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.001129
更新日期:2020-01-01 00:00:00
abstract::Purpose. In this study, we differentiated between tuberculosis (TB) and infections caused by nontuberculous mycobacteria (NTM) or Nocardia in a tertiary general hospital in China. Differences in clinical manifestations and factors between respiratory infections associated with these organisms were also investigated.Me...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000961
更新日期:2020-09-01 00:00:00
abstract::The rat was evaluated as an experimental model for disseminated candidosis by quantitating blood clearance and initial organ localisation of 3H-leucine-labelled Candida albicans after intravenous injection into the tail or portal vein. Viable or formalin-killed blastoconidia or viable blastoconidia with germ tubes wer...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-37-4-291
更新日期:1992-10-01 00:00:00
abstract::Certain bile acids inhibit the growth in vitro of organisms commonly found in the intestine and, in particular, anaerobic bacteria. As the importance of this effect in vivo has not been demonstrated, we investigated changes in intestinal flora in the rat after diversion of bile to the urinary bladder. Five days after ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-10-2-241
更新日期:1977-05-01 00:00:00
abstract::Three strains of Plesiomonas shigelloides isolated from patients with diarrhoea were agglutinated with Shigella flexneri 6 antiserum in slide and tube tests. All the strains were also agglutinated with a monoclonal antibody to the common group 1 antigen shared between S. flexneri serotypes and S. dysenteriae type 1. F...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-39-3-211
更新日期:1993-09-01 00:00:00
abstract::The characterization and tracking of pathogenic micro-organisms in the clinical laboratory and public health environment demand schemes that are easy to standardize and use, are automated and high-throughput, and provide portable data. A combined genomarkers approach for Salmonella enterica based on comparative sequen...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.047431-0
更新日期:2012-11-01 00:00:00
abstract::The growth of 29 strains of Acinetobacter spp. on semi-solid media was studied; 19 showed surface swarming and 14 produced channels ("ditches") in the agar that do not seem to have been described previously. An attempt was made to define the cultural and physical conditions for the demonstration of these phenomena. Po...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-8-3-443
更新日期:1975-08-01 00:00:00
abstract:PURPOSE:Diabetic patients seem to be predisposed to cutaneous candidiasis. In this study, we evaluated the interference of diabetic conditions in alloxan-induced diabetic mice in relation to the development of C. albicans infection, density of M1 and M2 macrophages, distribution of collagen type I and III and anti-infl...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000496
更新日期:2017-06-01 00:00:00
abstract::In the present study, the adhesion of bacteriocin-producing probiotic strains of Lactobacillus plantarum onto extracellular matrix (ECM) proteins such as collagen and mucin and their potential to prevent pathogen invasion onto the ECM was ascertained. Fluorescence-based in vitro assays indicated that L. plantarum stra...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000181
更新日期:2015-12-01 00:00:00
abstract::Seven isolates of B. piliformis, the agent of Tyzzer's disease, obtained from various host species, were examined for cytotoxic activity by incubating culture filtrates on BRL 3A rat-hepatocyte and 3T3 mouse-fibroblast cell lines. One isolate exhibited cytopathic effects on BRL 3A cells, but not on 3T3 cells. Three ot...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-37-1-77
更新日期:1992-07-01 00:00:00
abstract::Encapsulation of purified tuberculoproteins in liposomes augmented their ability to elicit delayed hypersensitivity reactions in BCG-immune rats. The effect was most marked with a low-molecular-weight tuberculopeptide that was relatively poor at eliciting reactions when in free form. These findings indicate that, in a...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-18-3-429
更新日期:1984-12-01 00:00:00
abstract::During a one-year period, 258 isolates of Campylobacter jejuni and C. coli were obtained from children with gastroenteritis or bacteraemia at the Red Cross Children's Hospital, Cape Town, South Africa. These isolates were biotyped by hippurate hydrolysis, H2S production and tolerance to 2,3,5-triphenyltetrazolium chlo...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-21-1-1
更新日期:1986-02-01 00:00:00
abstract::To gain a better understanding of the pathological role of interferon-gamma (IFN-gamma) in specific granuloma formation, IFN-gamma gene-deficient mice (BALB/c and C57BL/6) were produced. The IFN-gamma gene in embryonic stem (ES) cells was disrupted by inserting the beta-galactosidase gene (lacZ) and the neomycin resis...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-47-10-871
更新日期:1998-10-01 00:00:00