Abstract:
:Cell-surface hydrophobicity of Helicobacter (formerly Campylobacter) pylori was tested by aqueous two-phase partitioning and hydrophobic interaction chromatography. The hydrophobicity of H. pylori greatly exceeded that of Campylobacter fetus subsp. fetus, C. jejuni and Bacillus subtilis. A partition coefficient (PC) of hydrophobicity in the two-phase system was determined for H. pylori. PC was dependent on pH and the PC value was increased by greater than 20-fold at pH 2.5. Lithium cations increased PC, indicating a net negative surface charge. The presence of urea prevented the relative loss of hydrophobicity at raised pH. Exposure of H. pylori to proteolytic enzymes reduced the ability of the bacteria to adhere to human polymorphonuclear neutrophils (PMN). These findings suggest that H. pylori possesses protein-associated hydrophobic factors that are responsible for the non-opsonic adherence to PMN cell membranes.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Pruul H,Goodwin CS,McDonald PJ,Lewis G,Pankhurst Ddoi
10.1099/00222615-32-2-93subject
Has Abstractpub_date
1990-06-01 00:00:00pages
93-100issue
2eissn
0022-2615issn
1473-5644journal_volume
32pub_type
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