Abstract：
:The object of this study was to develop and characterize experimental Burkholderia mallei aerosol infection in BALB/c mice. Sixty-five mice were infected with 5000 [approx. 2.5 median lethal doses (MLD)] B. mallei strain ATCC 23344(T) bacteria by the aerosol route. Bacterial counts within lung, liver, spleen, brain, kidney and blood over 14 days were determined and histopathological and immunocytochemical profiles were assessed. Mortality due to B. mallei infection occurred between days 4 and 10 post-infection. Bacterial numbers were consistently higher in the lungs than in other tissues, reaching a maximum of approximately 1.0 x 10(6) c.f.u. ml(-1) at 5 days post-infection. Bacterial counts in liver and spleen tissue remained approximately equal, reaching a maximum of approximately 1.0 x 10(4) c.f.u. ml(-1) at day 4 post-infection. By day 14 post-infection, bacterial counts were in the range 1.0 x 10(3)-1.0 x 10(4) c.f.u. ml(-1) for all tissues. Infection of the lungs by B. mallei resulted in foci of acute inflammation and necrosis. As infection progressed, the inflammatory process became subacute or chronic; this was associated with the development of extensive consolidation. Lesions in liver and spleen tissue were typical of those that might be expected in bacteraemia or bacterial toxaemia. These results suggest that the BALB/c mouse is susceptible to B. mallei when delivered by the aerosol route and that this represents a model system of acute human glanders that is suitable for research into the pathogenesis of and vaccines against this disease.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Lever MS,Nelson M,Ireland PI,Stagg AJ,Beedham RJ,Hall GA,Knight G,Titball RWdoi
10.1099/jmm.0.05180-0keywords:
subject
Has Abstractpub_date
2003-12-01 00:00:00pages
1109-1115issue
Pt 12eissn
0022-2615issn
1473-5644journal_volume
52pub_type
杂志文章abstract：:We studied the immunoglobulin response to individual viral polypeptides in experimental primary and secondary infection with herpes simplex virus (HSV)-1 in mice. After a single footpad inoculation with 10(5.6) pfu of HSV-1, immunoglobulin to proteins mol. wts (10(3)) 44 and 75 appeared on day 7. Antibodies to gB, gC,...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-25-1-59
更新日期：1988-01-01 00:00:00
abstract：PURPOSE:Listeria monocytogenes is a genetically heterogeneous species, which is divided into evolutionary lineages and clonal complexes (CCs). Not all L. monocytogenes isolates are equally likely to cause disease, with CC1, and in particular sequence type (ST) 1, being the most prevalent complex in human and ruminant i...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000529
更新日期：2017-07-01 00:00:00
abstract：:Campylobacter strains isolated from the same stool sample were characterised by determination of biochemical properties and both heat-labile (Lior) and heat-stable (Lauwers) serotypes. In six of 60 campylobacter-infected stools, two or three strains differing in Lior-serotype were isolated from the same stool. In four...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-38-1-3
更新日期：1993-01-01 00:00:00
abstract：:Mice immunised by the oral or intraperitoneal route with a live aromatic-dependent strain of Salmonella typhimurium exhibited significantly less protection against oral challenge with 50 LD50 of an ovine isolate of S. typhimurium (12313) than when a bovine isolate with the same O antigens and phage-type as strain 1231...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-24-1-11
更新日期：1987-08-01 00:00:00
abstract：:The substrates inositol, rhamnose, d-tartrate and m-tartrate used in fermentation tests with 338 cultures of Salmonella paratyphi B differentiated strains in some phage types to give information that could be used in epidemiological investigations. Xylose in Bitter's medium, the fifth substrate by which 13 of a potent...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-26-4-285
更新日期：1988-08-01 00:00:00
abstract：:The annual prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in Malaysia has been estimated to be 30 % to 40 % of all S. aureus infections. Nevertheless, data on the antimicrobial resistance and genetic diversity of Malaysian MRSAs remain few. In 2009, we collected 318 MRSA strains from various wards of...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000387
更新日期：2016-12-01 00:00:00
abstract：:In recent years, hospitals in southeastern Europe have faced dramatically high rates of antibiotic-resistant Acinetobacter baumannii. We analysed the evolution of resistance among clinical isolates of A. baumannii group obtained from nine tertiary hospitals throughout Greece over 6 years (2010-2015). Identification an...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000708
更新日期：2018-04-01 00:00:00
abstract：:Iliopsoas abscess is usually secondary to the spread of infection from a contiguous focus. Primary disease is uncommon, except in children where Staphylococcus aureus is the main pathogen. We report a 60-year-old woman who developed a primary iliopsoas abscess as a result of haematogenous spread of Capnocytophaga sput...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.022905-0
更新日期：2010-11-01 00:00:00
abstract：:Soil specimens collected from a site around the home of patients with food-borne type E. botulism probably caused by neurotoxigenic Clostridium butyricum in Guanyun, Jiangsu province, China, were examined for the presence of neurotoxigenic C. butyricum. Five lakeside sites of Weishan lake, in an area near to the sites...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-48-2-133
更新日期：1999-02-01 00:00:00
abstract：:A total of 63 Aeromonas strains isolated from diarrhoeal faeces or water samples were tested for adhesion to HEp-2 cells. An association between diarrhoea and high level adhesion was observed in that 12 of the 34 faecal isolates and none of the 29 environmental isolates yielded greater than 20 bacteria per HEp-2 cell ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-26-1-19
更新日期：1988-05-01 00:00:00
abstract：:Nasal polyp cells in primary culture from cystic fibrosis (CF) and non-CF patients were compared for the ability to bind Pseudomonas aeruginosa cells and for the presence of sulphated glycoconjugates at the epithelial cell surface. Quantitation of bacterial adhesion, by scanning electronmicroscopy, showed no significa...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-36-2-104
更新日期：1992-02-01 00:00:00
abstract：:The present study aimed to characterize cell damage caused by vaginal Candida albicans isolates from women with different symptomatologies. It was evaluated 12 clinical isolates of C. albicans from vaginal samples: 4 from asymptomatic women (AS), 4 from women with a single episode of vulvovaginal candidiasis (VVC) and...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000547
更新日期：2017-08-01 00:00:00
abstract：:A down-flow immunoassay has been developed to detect serum mannan antigens, and the test was recently marketed as the Unimedi Candida monotest. Using 251 serum samples from 105 patients with candidaemia, a comparison of the Unimedi Candida monotest with the Cand-Tec latex agglutination test and 2 microplate enzyme imm...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.46314-0
更新日期：2006-05-01 00:00:00
abstract：:The distribution of hepatitis C virus (HCV) genotypes among Egyptian patients positive for anti-HCV was determined and their influence, when combined with neu-oncoprotein overexpression, on the development of hepatocellular carcinoma (HCC) was examined. The study groups included asymptomatic carriers (ASC) and patient...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/0022-1317-49-1-89
更新日期：2000-01-01 00:00:00
abstract：:Serum opacity factor of Streptococcus suis (OFS) has recently been identified as a virulence determinant of an S. suis strain. In this study, we investigated the prevalence and variations of the ofs gene among 108 S. suis isolates from diseased and healthy pigs, and human patients. PCR screening and sequencing analysi...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.47755-0
更新日期：2008-04-01 00:00:00
abstract：:Antibodies (Abs) to the protective antigen (PA) component of the anthrax toxins have anti-spore as well as anti-toxin activities. Anti-PA antisera and purified anti-PA Abs enhance the phagocytosis by murine-derived macrophages (MQs) of spores of the Ames and Sterne strains and retard the germination of extracellular s...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/0022-1317-51-10-821
更新日期：2002-10-01 00:00:00
abstract：:We sought evidence to determine if particular strains of Pseudomonas aeruginosa have a predilection for pulmonary colonisation in patients with cystic fibrosis (CF). The incidence of common pyocin types in non-CF isolates (74%) was similar to that noted in previous reports but differed significantly (X2 = 16.7, p less...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-32-3-169
更新日期：1990-07-01 00:00:00
abstract：:The effect of various energy sources and metabolic intermediates on the attachment of Treponema pallidum to baby rabbit genital organ (BRGO) cells in culture was examined. Pyruvate and glucose enhanced the motility of T. pallidum in vitro. Pyruvate increased significantly the attachment of treponemes to BRGO cells whe...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-17-2-121
更新日期：1984-04-01 00:00:00
abstract：:A novel spirochaete was isolated from a case of severe virulent ovine foot rot (SVOFR) by immunomagnetic separation with beads coated with polyclonal anti-treponemal antisera and prolonged anaerobic broth culture. The as yet unnamed treponeme differs considerably from the only other spirochaete isolated from ovine foo...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/0022-1317-50-12-1061
更新日期：2001-12-01 00:00:00
abstract：:The effects of imipenem and meropenem on the transcriptional expression of resistance-related genes oprD, oprM and oprN in Pseudomonas aeruginosa were studied by quantitative real-time PCR. Four strains were examined: the type strain PT5 (PAO1), its derivatives M7 and PT149, and a clinical isolate, PaKT3. The derivati...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.45692-0
更新日期：2004-09-01 00:00:00
abstract：:A large molecular-weight fraction of Pseudomonas aeruginosa culture filtrate protected rats and mice against a lethal infection with a heterologous serotype, and to some extent against Escherichia coli and Listeria monocytogenes. The active components were obtained from cultures grown for several days in a simple synt...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-13-2-201
更新日期：1980-05-01 00:00:00
abstract：:The bactericidal action of non-immune whole blood on Proteus mirabilis was increased when the bacteria contained the R-plasmid RP1. This effect was due mainly to increased phagocytosis. Iron-depleted stationary-phase cells were more sensitive than carbon-depleted cells. The contribution of serum was usually negligible...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-24-3-233
更新日期：1987-11-01 00:00:00
abstract：:The family Adenoviridae consists of five genera of which the genus Mastadenovirus includes human viruses classified into 57 serotypes clustered into seven subgroups (A-G). Serotypes 40 and 41 (subgroup F) are specifically associated with childhood gastroenteritis and are the most common cause of acute gastroenteritis ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.072413-0
更新日期：2014-06-01 00:00:00
abstract：:Rosacea is a chronic inflammatory condition that affects the skin of the face and the eyes. The aetiology of rosacea is not clearly established but increasing evidence suggests a potential role for bacteria in the induction of the condition. A role for Bacillus oleronius, originally isolated from within a Demodex foll...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.065136-0
更新日期：2014-02-01 00:00:00
abstract：:Clostridium botulinum type A toxin is the most prevalent cause of naturally occurring outbreaks of human botulism in the world. The active dichain neurotoxin molecule is composed of a heavy chain (H-chain) of ~100 kDa with the carboxy-terminal end consisting of a receptor-binding (HC) domain, while the amino-terminal ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.053124-0
更新日期：2013-06-01 00:00:00
abstract：:The increasing incidence of reported scabies in Trinidad, from 24.2/100,000 population in 1984 to 59.5/100,000 in 1985, led to a careful monitoring in 1986 of all patients with post-streptococcal acute glomerulonephritis (PSAGN). There were 181 cases of PSAGN; 84 beta-haemolytic streptococcal isolates from 72 patients...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-32-2-111
更新日期：1990-06-01 00:00:00
abstract：:Since 2007, there has been a re-emergence of cholera outbreaks in northern Vietnam. To understand the molecular epidemiological relatedness and determine the antibiotic susceptibility profiles of responsible V. cholerae O1 outbreak strains, a representative collection of 100 V. cholerae O1 strains was characterized. V...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.034744-0
更新日期：2012-03-01 00:00:00
abstract：PURPOSE:The aim of this study was to assess the biotope of the Cryptococcus neoformans/Cryptococcus gattii species complex from Ivory Coast, and clarify the possible epidemiological relationship between environmental and clinical strains. METHODOLOGY:Samples from Eucalyptus camaldulensis (n=136), Mangifera indica (n=1...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000654
更新日期：2018-01-01 00:00:00
abstract：PURPOSE:Infections associated with Acinetobacter baumannii represent an increasing threat in healthcare settings. Therefore, we investigated the epidemiological relationship between clinical isolates of A. baumannii obtained from patients in a university hospital in Bandar Abbas in southern Iran. METHODOLOGY:Sixty-fou...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000619
更新日期：2017-11-01 00:00:00
abstract：:In-vitro proteinase production by oral Candida albicans isolates from patients with and without HIV infection (18 isolates from each group) was assessed by image analysis of a plate assay, with bovine serum albumin (BSA) as a substrate. The effect of sub-minimal inhibitory concentrations (sub-MICs) of nystatin, amphot...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-44-4-311
更新日期：1996-04-01 00:00:00
