Abstract：
:The effect of various energy sources and metabolic intermediates on the attachment of Treponema pallidum to baby rabbit genital organ (BRGO) cells in culture was examined. Pyruvate and glucose enhanced the motility of T. pallidum in vitro. Pyruvate increased significantly the attachment of treponemes to BRGO cells when compared with the other substrates but all substrates tested stimulated DNA synthesis by cultured BRGO cells. Thus, the effect of pyruvate on attachment may be due to an effect on the treponemes. Prior exposure of the BRGO cells to the glucose analogue 2-deoxyglucose greatly inhibited the attachment of T. pallidum whereas three other analogues had no effect. The inhibitory effect of 2-deoxyglucose was partially reversed by the presence of pyruvate in the attachment assay. These results suggest that energy metabolism of both T. pallidum and host cells may be required for the initial interaction of T. pallidum with its host in vivo.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Wong GH,Steiner BMdoi
10.1099/00222615-17-2-121subject
Has Abstractpub_date
1984-04-01 00:00:00pages
121-8issue
2eissn
0022-2615issn
1473-5644journal_volume
17pub_type
杂志文章abstract：:The aim of this study was to evaluate the presence and prevalence of multidrug antibacterial resistance in Helicobacter pylori in Bulgaria from 2005 to 2008. The resistance in 828 untreated adults, 124 treated adults and 105 untreated children was, respectively, 26.5, 50.8 and 16.2% for metronidazole; 18.4, 45.2 and 1...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.009993-0
更新日期：2009-07-01 00:00:00
abstract：:A novel combination of culturing and DNA-based terminal restriction fragment length polymorphism (TRFLP) analysis was used to investigate the effect of probiotics on antibiotic-induced gut microbiota alterations to determine if a probiotic preparation containing bifidobacteria and lactobacilli, taken during and after ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.47615-0
更新日期：2009-05-01 00:00:00
abstract：:A simple method is described for testing milk specimens from nursing mothers for the presence of RNA-dependent DNA-polymerase activity. Positive results were obtained in five of 137 women (3.6%) without a family history of breast cancer, and in six of 31 women (19.3%) with a family history of breast cancer. ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-8-3-447
更新日期：1975-08-01 00:00:00
abstract：:Various factors influencing the susceptibility of C3H mice to lethal infection by the phycomycete Absidia ramosa were examined. Mice 19-21 days old were exposed to graded doses of A. ramosa spores by various routes. After intravenous inoculation with doses in excess of 10(3) viable units, a variable proportion of mice...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-8-4-551
更新日期：1975-11-01 00:00:00
abstract：:There is great interest in the use of antimicrobial agents for the prevention and treatment of plaque-related oral diseases and many publications have reported the results of studies in which the minimum inhibitory concentrations of agents for cariogenic and periodontopathogenic bacteria have been determined. However,...
journal_title：Journal of medical microbiology
pub_type： 杂志文章,评审
doi：10.1099/00222615-44-2-79
更新日期：1996-02-01 00:00:00
abstract：:Eight monoclonal antibodies (MAbs) were produced from mice immunised with whole cells of heat-killed Pasteurella multocida type A which had been cultured under iron-restricted conditions. The MAbs were selected by an enzyme-linked immunosorbent assay (ELISA) in which the antigen consisted of whole bacteria of the immu...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-33-4-217
更新日期：1990-12-01 00:00:00
abstract：:Enteroaggregative Escherichia coli (EAEC) is an important agent of diarrhoeal diseases worldwide. The role of EAEC virulence factors in the clinical outcome of infection is not completely defined. This case-control study investigated the prevalence of EAEC, its virulence genes and the antimicrobial resistance profile ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000055
更新日期：2015-05-01 00:00:00
abstract：:Limited digestion of staphylococcal enterotoxin B (SEB) with trypsin resulted in the generation of a 12-Kda amino-terminal fragment and a 17-Kda carboxy-terminal fragment which were isolated by preparative iso-electric focusing. The carboxy-terminal fragment exhibited significant mitogenic activity for murine splenocy...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-36-3-155
更新日期：1992-03-01 00:00:00
abstract：:The resistance of Balamuthia mandrillaris to physical, chemical and radiological conditions was tested. Following treatments, viability was determined by culturing amoebae on human brain microvascular endothelial cells for up to 12 days. B. mandrillaris cysts were resistant to repeated freeze-thawing (five times), tem...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.47694-0
更新日期：2008-04-01 00:00:00
abstract：:A 29-year-old pregnant woman was admitted to hospital with signs of sepsis and threatened pre-term labour. The premature neonate also showed signs of sepsis. Haemophilus influenzae biotype III was cultured from a midstream urine sample taken from the mother, maternal placental swabs and neonatal blood cultures. The pl...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.016543-0
更新日期：2010-03-01 00:00:00
abstract：:Campylobacter-like organisms, isolated from the gastric antrum of Rhesus monkeys, were compared with Campylobacter jejuni and C. pylori. They were similar to C. pylori by light microscopy, in ultrastructural morphology, in enzymic, fatty-acid-methyl-ester, and protein-profile analysis, and in antigenic reactivity with...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-27-1-41
更新日期：1988-09-01 00:00:00
abstract：:Preliminary differentiation of Leptospira interrogans serovars hardjo and balcanica can be made with antisera that are collected from hamsters after experimental infection with serovar hardjo and selected for high homologous and low or nil heterologous titres, as measured by the microscopic agglutination test. Rabbit ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-11-3-351
更新日期：1978-08-01 00:00:00
abstract：:A total of 110 clinical isolates of Vibrio cholerae O1 biotype El Tor serotype Ogawa isolated in a recent outbreak from different districts of Teheran, Iran, was subjected to 99 carbon source utilisation tests, ribotyping and toxinogenotyping. PCR showed that the genes encoding cholera toxin (ctxA), toxin co-regulated...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/0022-1317-49-12-1085
更新日期：2000-12-01 00:00:00
abstract：:In this study, the species diversity of staphylococci with inducible resistance to macrolides, lincosamides and streptogramin B (MLSB) isolated from clinical samples, sewage and river water was investigated. Inducible clindamycin resistance was tested using a D-test and macrodilution assays. Inducible cross-resistance...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.077081-0
更新日期：2014-11-01 00:00:00
abstract：PURPOSE:This study investigates the virulence and antimicrobial resistance in association with common clonal complexes (CCs) of enteroaggregative Escherichia coli (EAEC) isolated from Bangladesh. The aim was to determine whether specific CCs were more likely to be associated with putative virulence genes and/or antimic...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000594
更新日期：2017-10-01 00:00:00
abstract：:A rabbit was immunised with a heat-killed vaccine prepared from strain 1142 of Staphylococcus epidermidis. The HA titres of the antisera against a surface-polysaccharide antigen extracted from the homologous strain were highest in the 2nd-4th weeks after immunisation and subsequently declined. Only a minor amount of 2...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-11-4-371
更新日期：1978-11-01 00:00:00
abstract：:Anaplasma phagocytophilum, an agent of human granulocytic anaplasmosis, infects neutrophils and causes an emerging tickborne febrile disease. The genome of this bacterium contains a large number of p44/msp2-related genes encoding 44 kDa major outer-membrane proteins, and it is known that a specific p44/msp2 gene is pr...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.011775-0
更新日期：2009-12-01 00:00:00
abstract：:A total of 95 isolates, belonging to 33 species of five dermatophyte genera, i.e. Arthroderma (15 species), Chrysosporium (two), Epidermophyton (one), Microsporum (three) and Trichophyton (12), were studied using internal transcribed spacer 2 (ITS2)-PCR-RFLP analysis (ITS2-RFLP), consisting of amplification of the ITS...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.013870-0
更新日期：2010-01-01 00:00:00
abstract：:A rapid and simple method for counting viable bacteria is described. The technique involves the use of a semi-automatic micropipette and disposable glass-capillary tubes. Viable counts obtained by this method were not significantly different from those obtained by the Miles, Misra and Irwin method or by a pour-plate m...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-11-4-541
更新日期：1978-11-01 00:00:00
abstract：:The susceptibilities of Actinobacillus actinomycetemcomitans cultures, grown as 1- or 3-day-old biofilms or as planktonic suspensions, to concentrations of chlorhexidine digluconate, cetylpyridinium chloride or triclosan used in commercial mouthwashes were compared. Three-day biofilms were the most resistant form of t...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-46-5-425
更新日期：1997-05-01 00:00:00
abstract：PURPOSE:Urinary tract infection (UTI) can be community-acquired (Com-UTI) or catheter-associated (CAUTI) and may be associated with biofilm-producing organisms. A comparative analysis of biofilm-producing property (BPP), antibiotic-sensitivity and multi-drug resistance (MDR) and their relation with the BPP of isolates ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000525
更新日期：2017-07-01 00:00:00
abstract：PURPOSE:An observational study was performed to investigate the carriage rate and serotypes of Streptococcus pneumoniae in the 13-valent pneumococcal conjugate vaccine (PCV13) era in Taiwan. METHODOLOGY:From March 2014 to March 2015 a total of 500 healthy children and their households (631 adults) were enrolled from t...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000488
更新日期：2017-06-01 00:00:00
abstract：:The genetic characteristics of Vibrio parahaemolyticus strains isolated in 2004 and 2005 in Mozambique were assessed in this study to determine whether the pandemic clone of V. parahaemolyticus O3 : K6 and O4 : K68 serotypes has spread to Mozambique. Fifty-eight V. parahaemolyticus strains isolated from hospitalized d...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.2008/004275-0
更新日期：2008-12-01 00:00:00
abstract：:The cystic fibrosis (CF) airways are colonized by polymicrobial communities with high bacterial load and are influenced by frequent antibiotic exposures. This community includes diverse streptococci, some of which have been directly or indirectly associated with pulmonary exacerbations. As many streptococci are natura...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000172
更新日期：2015-11-01 00:00:00
abstract：:Diabetic mice were significantly more susceptible than normal mice to bladder infection with Enterobacter cloacae or Serratia marcescens but not to intraperitoneal infection. Normal urine inhibited the growth of E. cloacae and S. marcescens, whereas urine from diabetic mice permitted multiplication. The addition of ur...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-30-2-105
更新日期：1989-10-01 00:00:00
abstract：:Rapid identification of isolates in positive blood cultures are of great importance to secure correct treatment of septicaemic patients. As antimicrobial resistance is increasing, rapid detection of resistance is crucial. Carbapenem resistance in Bacteroides fragilis associated with cfiA-encoded class B metallo-beta-l...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.075465-0
更新日期：2014-08-01 00:00:00
abstract：:The incidence of candidaemia among immunocompromised patients in Malaysia is increasing at an alarming rate. Isolation of clinical strains that are resistant to fluconazole has also risen markedly. We report here the repeated isolation of Candida tropicalis from the blood of a neonatal patient with Hirschsprung's dise...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.46045-0
更新日期：2006-04-01 00:00:00
abstract：:Multilocus sequence typing (MLST) is considered a reliable method for providing insight into the Streptococcus suis population structure, clonal relationships and the potential of particular clones to cause disease. Indeed, MLST has revealed the presence of several clonal complexes (CCs) within the Streptococcus suis ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000239
更新日期：2016-05-01 00:00:00
abstract：:In this study, 250 vaginal samples from patients with vulvovaginal candidosis were inoculated onto two chromogenic media, Albicans ID(2) and Biggy agar, as well as onto Sabouraud chloramphenicol agar, yielding a total of 63 yeast (25.2 %) on all three media. These strains were identified as Candida glabrata in 20 (31....
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.46625-0
更新日期：2007-06-01 00:00:00
abstract：:The nucleolar accumulation of Semliki Forest Virus (SFV) C protein was examined as a function of intact microtubules, intact microfilaments and accessible intermediate filaments. The cytoskeletal components do not seem to play a role in directing C protein to the nucleolus but nucleolar accumulation is energy-dependen...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-40-6-389
更新日期：1994-06-01 00:00:00
