Evaluation of a newly developed down-flow immunoassay for detection of serum mannan antigens in patients with candidaemia.

abstract：PURPOSE:Listeria monocytogenes is a genetically heterogeneous species, which is divided into evolutionary lineages and clonal complexes (CCs). Not all L. monocytogenes isolates are equally likely to cause disease, with CC1, and in particular sequence type (ST) 1, being the most prevalent complex in human and ruminant i...

journal_title：Journal of medical microbiology

authors： Rupp S,Bärtschi M,Frey J,Oevermann A

更新日期：2017-07-01 00:00:00

abstract：:Polyacrylamide-agarose gel electrophoresis was used to study polymorphism of lactate dehydrogenase (LDH), nucleoside phosphorylase (NSP), phosphoglucose isomerase (PGI), hydroxybutyrate dehydrogenase (HBD), adenylate kinase (ADK) and esterases of 44 strains of Streptococcus pyogenes, 25 group G streptococcal strains, ...

journal_title：Journal of medical microbiology

authors： Bert F,Picard B,Lambert-Zechovsky N,Goullet P

更新日期：1995-06-01 00:00:00

abstract：:Klebsiella pneumoniae meningitis has recently become an increasingly common cause of central nervous system infection. The invasion of bacteria within the subarachnoid space stimulates the release of pro-inflammatory cytokines and chemokines, triggering a host immune response. The aim of the present study was to evalu...

journal_title：Journal of medical microbiology

authors： Barichello T,Simões LR,Valvassori SS,Generoso JS,Aveline PEDV,Dominguini D,Elias SG,Vilela MC,Quevedo J,Teixeira AL

更新日期：2014-01-01 00:00:00

abstract：:The aim of this study was to improve the identification of Mycobacterium species in the context of a UK teaching hospital. Real-time PCR assays were established to enable the rapid differentiation between Mycobacterium tuberculosis (MTB) complex and Mycobacterium species other than tuberculosis (MOTT), followed by 16S...

journal_title：Journal of medical microbiology

authors： Williams KJ,Ling CL,Jenkins C,Gillespie SH,McHugh TD

更新日期：2007-05-01 00:00:00

abstract：:Spondylodiscitis caused by Aggregatibacter aphrophilus, formerly known as Haemophilus paraphrophilus, is an unusual condition and can be very difficult to diagnose. We report a case of cervical spondylodiscitis complicated by spinal epidural abscess in a 63-year-old woman, without underlying predisposing conditions. T...

journal_title：Journal of medical microbiology

authors： Pasqualini L,Mencacci A,Scarponi AM,Leli C,Fabbriciani G,Callarelli L,Schillaci G,Bistoni F,Mannarino E

更新日期：2008-05-01 00:00:00

abstract：:Two monoclonal antibodies (MAbs) to a 36-kDa extracellular metalloprotease (PSCP) from Burkholderia (Pseudomonas) cepacia were found to react with thermolysin, Pseudomonas aeruginosa elastase, alkaline protease (Apr) and LasA, Serratia marcescens protease (SMP), Aeromonas hydrophila protease (AhP), and both the lethal...

journal_title：Journal of medical microbiology

authors： Kooi C,Sokol PA

更新日期：1996-09-01 00:00:00

abstract：:The effects of ethyleneglycoltetra-acetic acid (EGTA) and EGTA + magnesium (MgEGTA) on the viable counts of 10 strains of Escherichia coli O6 have been studied in normal human serum (NHS), heat-inactivated serum (HIS) and in culture media with and without the addition of a beta-lactam antibiotic. The addition of EGTA ...

journal_title：Journal of medical microbiology

authors： Roberts AP,Phillips R

更新日期：1981-05-01 00:00:00

abstract：:The encapsulated strain M of Staphylococcus aureus and the encapsulated strain 76 of S. simulans survived incubation with bovine neutrophils in media containing heated bovine serum 0.5% v/v or milk whey 10% v/v. With two unencapsulated strains of S. aureus, M12 and M60, less than 5% of the original inoculum survived. ...

journal_title：Journal of medical microbiology

authors： Anderson JC,Williams MR

更新日期：1985-12-01 00:00:00

abstract：:Neisseria sicca is rarely associated with clinical infections and to the authors' knowledge this organism has not been reported as a causative agent of infected biloma (liver abscess). A case of a diabetic man with infected biloma due to N. sicca after repeated transcatheter arterial embolization for hepatocellular ca...

journal_title：Journal of medical microbiology

authors： Chung HC,Teng LJ,Hsueh PR

更新日期：2007-11-01 00:00:00

abstract：:Helicobacter pylori is believed to trigger neutrophil activation through several factors, including the H. pylori neutrophil-activating protein (HpNAP). The aim of this study was to characterise the factors within H. pylori cell-free extracts that stimulate neutrophil activation. Neutrophil activation was found to be ...

journal_title：Journal of medical microbiology

authors： Leakey A,Hirst R,LA Brooy J

更新日期：2001-09-01 00:00:00

abstract：PURPOSE:We previously identified an association between CC22 meticillin-resistant Staphylococcus aureus (MRSA) bloodstream infection isolates with an elevated vancomycin MIC (V-MIC) in the susceptible range (1.5-2 mg l-1) and endocarditis. This study explores whether these isolates have a specific phenotype consistent ...

journal_title：Journal of medical microbiology

authors： Marbach H,Boakes E,Lynham S,Ward M,Otter JA,Edgeworth JD

更新日期：2017-05-01 00:00:00

abstract：:Neisseria gonorrhoeae expresses receptors for both lactoferrin (LF) and transferrin (TF). To determine whether qualitative or quantitative changes in these receptors, or both, correlate with the pattern of disease due to N. gonorrhoeae, a dot binding assay with whole cells was used to measure the absolute receptor lev...

journal_title：Journal of medical microbiology

authors： Lee BC,Bryan LE

更新日期：1989-03-01 00:00:00

abstract：:The aim of the present study was to correlate molecular evidence of the presence of Helicobacter pylori in gastric biopsy samples, based on analysis of 16S rDNA, vacuolating toxin (vacA), urease A (ureA) and cagA genes, with the clinical, histological and serological findings in patients with H. pylori-associated gast...

journal_title：Journal of medical microbiology

authors： Tiveljung A,Borch K,Jonasson J,Mårdh S,Petersson F,Monstein HJ

更新日期：1998-08-01 00:00:00

abstract：:Five hundred clinical group A streptococcal (GAS) isolates were collected in Belgium during the period 1 Nov. 1993 to 31 Oct. 1994. Clinical and laboratory data were recorded and isolates were characterised. The presence of the genes encoding streptococcal pyrogenic exotoxin types A (speA), B (speB), C (speC), F (speF...

journal_title：Journal of medical microbiology

authors： Descheemaeker P,VAN Loock F,Hauchecorne M,Vandamme P,Goossens H

更新日期：2000-05-01 00:00:00

abstract：:An earlier study reported that human cerebrospinal fluid (CSF) has fungistatic activity for Cryptococcus neoformans. The present study reports that molecular sieve fractionation of concentrated CSF yielded three protein peaks, one of which (p2) had anticryptococcal activity. On a DEAE-Sephacel anion-exchange column th...

journal_title：Journal of medical microbiology

authors： Ahluwalia M,Brummer E,Sridhar S,Singh R,Stevens DA

更新日期：2001-01-01 00:00:00

abstract：:Cytotoxin (CTX) production in 34 human and 22 animal strains of Campylobacter jejuni isolated in Japan and other countries was studied by three assay systems described previously. Furthermore, cholera-like enterotoxin production by these strains was tested by reversed passive latex agglutination (RPLA). CTX titres in ...

journal_title：Journal of medical microbiology

authors： Misawa N,Ohnishi T,Itoh K,Takahashi E

更新日期：1995-11-01 00:00:00

abstract：:Cellular fatty acid analysis by GLC is widely used in the species identification of mycobacteria. Combining mycolic acid cleavage products with shorter cellular fatty acids increases the informative value of the analysis. A key has been created to aid in the identification of all currently known slowly growing environ...

journal_title：Journal of medical microbiology

authors： Torkko P,Katila ML,Kontro M

更新日期：2003-04-01 00:00:00

abstract：:The distribution of cryptic plasmids among 123 isolates of Providencia stuarti from a hospital ward during a prospective epidemiological study is reported. Two closely related stable plasmids (34 Kb and 36 Kb) were identified by restriction endonuclease digest analysis of plasmid DNA. One or other of these cryptic pla...

journal_title：Journal of medical microbiology

authors： Hawkey PM,Bennett PM,Hawkey CA

更新日期：1984-10-01 00:00:00

abstract：PURPOSE:Diabetic patients seem to be predisposed to cutaneous candidiasis. In this study, we evaluated the interference of diabetic conditions in alloxan-induced diabetic mice in relation to the development of C. albicans infection, density of M1 and M2 macrophages, distribution of collagen type I and III and anti-infl...

journal_title：Journal of medical microbiology

authors： Pupim ACE,Campois TG,Araújo EJA,Svidizinski TIE,Felipe I

更新日期：2017-06-01 00:00:00

abstract：:Introduction. Ocular microsporidiosis is a significant emerging infectious disease reported in immunocompromised patients and immunocompetent persons throughout the world.Aim. To identify the pathogens responsible for human keratitis, via corneal scrapings.Methodology. Thirty-three hospitalized patients with epithelia...

journal_title：Journal of medical microbiology

authors： Sanpool O,Thanathanee O,Laummuanwai P,Maleewong W,Intapan PM

更新日期：2020-03-01 00:00:00

abstract：:An affinity procedure with purified, biotinylated human transferrin and streptavidin-agarose was used to identify the transferrin-binding proteins in strains of Haemophilus influenzae. Proteins of 58 and 98 Kda were isolated from total membranes prepared from iron-deficient but not iron-sufficient H. influenzae KC548 ...

journal_title：Journal of medical microbiology

authors： Schryvers AB

更新日期：1989-06-01 00:00:00

abstract：:Eight monoclonal antibodies (MAbs) were produced from mice immunised with whole cells of heat-killed Pasteurella multocida type A which had been cultured under iron-restricted conditions. The MAbs were selected by an enzyme-linked immunosorbent assay (ELISA) in which the antigen consisted of whole bacteria of the immu...

journal_title：Journal of medical microbiology

authors： Wijewardana TG,Wilson CF,Gilmour NJ,Poxton IR

更新日期：1990-12-01 00:00:00

abstract：:This study investigated the comparative performance of the Amplicor assay and an in-house semi-automated, multiplex real-time PCR for the diagnosis of genital chlamydial infection. Four different assays, the COBAS Amplicor CT test (Amplicor PCR), in-house real-time PCR (IHRT-PCR), in-house nested cryptic plasmid PCR a...

journal_title：Journal of medical microbiology

authors： Jalal H,Al-Suwaine A,Stephen H,Carne C,Sonnex C

更新日期：2007-03-01 00:00:00

abstract：:Data on the occurrence and epidemiology of Aspergillus spp. in laying hens farms are scant. With the aims of determining levels of airborne contamination in laying hen farms and evaluating the potential risk of infection for workers and animals, 57 air samples from 19 sheds (Group I), 69 from faeces (Group II), 19 fro...

journal_title：Journal of medical microbiology

authors： Cafarchia C,Camarda A,Iatta R,Danesi P,Favuzzi V,Di Paola G,Pugliese N,Caroli A,Montagna MT,Otranto D

更新日期：2014-03-01 00:00:00

abstract：:Early diagnosis of leptospirosis is important because severe leptospiral infection can run a fulminant course. The polymerase chain reaction (PCR) was evaluated for the detection of leptospires in clinical samples from patients with acute leptospiral infection. Blood and urine samples from 71 patients with leptospiros...

journal_title：Journal of medical microbiology

authors： Brown PD,Gravekamp C,Carrington DG,van de Kemp H,Hartskeerl RA,Edwards CN,Everard CO,Terpstra WJ,Levett PN

更新日期：1995-08-01 00:00:00

abstract：:Rapid identification of the causative bacteria of sepsis in patients can contribute to the selection of appropriate antibiotics and improvement of patients' prognosis. Genotypic identification is an emerging technology that may provide an alternative method to, or complement, established phenotypic identification proc...

journal_title：Journal of medical microbiology

authors： Motoshima M,Yanagihara K,Morinaga Y,Matsuda J,Hasegawa H,Kohno S,Kamihira S

更新日期：2012-11-01 00:00:00

abstract：:The aim of this study was the analysis of the cytokine response in BALB/c mice infected with the highly virulent RH or the weakly virulent Beverley strains of Toxoplasma gondii. Analysis of cytokine messages showed increased expression of IL12, IFN-gamma and TNF-alpha, but not IL4 mRNAs in spleen cells after infection...

journal_title：Journal of medical microbiology

authors： Nguyen TD,Bigaignon G,Markine-Goriaynoff D,Heremans H,Nguyen TN,Warnier G,Delmee M,Warny M,Wolf SF,Uyttenhove C,Van Snick J,Coutelier JP

更新日期：2003-10-01 00:00:00

abstract：:Bacterial activity and growth were monitored by following the changes of electrical impedance of cultures in liquid media. The signal is expressed automatically as a curve similar to grwoth curves produced by other methods. The technique offers a new, rapid and sensitive means of detecting active micro-organisms and i...

journal_title：Journal of medical microbiology

authors： Ur A,Brown DF

更新日期：1975-02-01 00:00:00

abstract：:Iliopsoas abscess is usually secondary to the spread of infection from a contiguous focus. Primary disease is uncommon, except in children where Staphylococcus aureus is the main pathogen. We report a 60-year-old woman who developed a primary iliopsoas abscess as a result of haematogenous spread of Capnocytophaga sput...

journal_title：Journal of medical microbiology

authors： Chan JFW,Wong SSY,Leung SSM,Li IWS,To KKW,Cheng VCC,Yuen KY

更新日期：2010-11-01 00:00:00

abstract：:Meticillin-resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen in Pakistan and is emerging in the community. This is one of the first reports of the prevalent genotypes of MRSA in both hospital and community settings in Pakistan. Isolates collected in 2006-2007 were characterized by PFGE, staphylococ...

journal_title：Journal of medical microbiology

authors： Zafar A,Stone M,Ibrahim S,Parveen Z,Hasan Z,Khan E,Hasan R,Wain J,Bamford K

更新日期：2011-01-01 00:00:00