Cytotoxin detection in Campylobacter jejuni strains of human and animal origin with three tissue culture assay systems.

abstract：:We present a case of septicaemia caused by Arcanobacterium haemolyticum in a previously healthy 23-year-old man suffering from acute pharyngotonsillitis, who developed complicated Lemierre's syndrome. Three blood cultures (both aerobic and anaerobic) revealed the exclusive presence of A. haemolyticum. The presence of ...

journal_title：Journal of medical microbiology

authors： Fernández-Suárez A,Aguilar Benítez JM,López Vidal AM,Díaz Iglesias JM

更新日期：2009-12-01 00:00:00

abstract：:A collection of 72 strains of catalase-negative gram-positive, -negative and -variable cocco-bacilli isolated from samples of vaginal discharge from women with non-specific vaginal infection was examined in an attempt to develop an identification system for Gardnerella vaginalis that could be used in a diagnostic labo...

journal_title：Journal of medical microbiology

authors： Taylor E,Phillips I

更新日期：1983-02-01 00:00:00

abstract：:The contribution of quorum sensing in some phenotypic and pathogenic characteristics of Pseudomonas aeruginosa was studied. The production of acylhomoserine lactones (AHL) by planktonic cultures of eight clinical and reference strains of P. aeruginosa was evaluated using two biosensors. The adhesion of the bacteria on...

journal_title：Journal of medical microbiology

authors： Nagant C,Seil M,Nachtergael A,Dulanto S,Dehaye JP

更新日期：2013-07-01 00:00:00

abstract：:The ability of Mycobacterium leprae to specifically bind alpha2-laminins of Schwann cells has been described recently as being an important property of the leprosy bacillus, which could explain the neural tropism of M. leprae. Therefore, the extent of the expression of alpha2-laminin-binding properties among mycobacte...

journal_title：Journal of medical microbiology

authors： Marques MA,Ant nio VL,Sarno EN,Brennan PJ,Pessolani MC

更新日期：2001-01-01 00:00:00

abstract：:This study investigated the comparative performance of the Amplicor assay and an in-house semi-automated, multiplex real-time PCR for the diagnosis of genital chlamydial infection. Four different assays, the COBAS Amplicor CT test (Amplicor PCR), in-house real-time PCR (IHRT-PCR), in-house nested cryptic plasmid PCR a...

journal_title：Journal of medical microbiology

authors： Jalal H,Al-Suwaine A,Stephen H,Carne C,Sonnex C

更新日期：2007-03-01 00:00:00

abstract：:Thirty-six closed abcesses in the subcutis of cats were examined. Of 168 bacterial strains isolated, 121(72%) were anaerobes and 47 (28%) were facultative anaerobes. Twenty-six abscesses contained mixtures of facultative anaerobes and anaerobes, six contained anaerobes only and four contained facultative anaerobes onl...

journal_title：Journal of medical microbiology

authors： Love DN,Jones RF,Bailey M,Johnson RS

更新日期：1979-05-01 00:00:00

abstract：:The purpose of this study was to compare the frequency of recovery of aerobic and anaerobic organisms with interfering capability against group A beta-haemolytic streptococci (GABHS) and beta-lactamase-producing bacteria (BLPB) from the tonsils of GABHS carriers and non-carriers. The presence of aerobic and anaerobic ...

journal_title：Journal of medical microbiology

authors： Brook I,Gober AE

更新日期：2006-12-01 00:00:00

abstract：:Introduction. Neonatal infection with Cronobacter sakazakii can cause severe intestinal damage and necrotizing enterocolitis (NEC). The inflammasome and Toll-like receptors mediate intestinal damage caused by other intestinal pathogens causing NEC, but the exact mechanism is unclear.Aim. We evaluated the molecular mec...

journal_title：Journal of medical microbiology

authors： Chen Z,Zhang Y,Lin R,Meng X,Zhao W,Shen W,Fan H

更新日期：2020-05-01 00:00:00

abstract：:The faecal flora and mucosa-associated flora (MAF) of rectal biopsy material from 12 patients with active Crohn's disease were studied before and during treatment with a combination of metronidazole and cotrimoxazole given orally for at least 2 weeks. The total faecal flora was greater than the MAF although the propor...

journal_title：Journal of medical microbiology

authors： Hudson MJ,Hill MJ,Elliott PR,Berghouse LM,Burnham WR,Lennard-Jones JE

更新日期：1984-12-01 00:00:00

abstract：:Stenotrophomonas maltophilia KH has two acid beta-lactamases with isoelectric points (pIs) of 4.6 and 5.4, and several basic beta-lactamases (pIs >7.0) that produce a ladder-shaped pattern by IEF. An isogenic L2 mutant, KHL2(xylE), was constructed by gene replacement. From IEF and native PAGE zymograms of strains KH a...

journal_title：Journal of medical microbiology

authors： Hu RM,Chiang KH,Chang YC,Yang TC

更新日期：2009-03-01 00:00:00

abstract：:In order to develop novel host/pathogen real-time PCR assays for routine diagnostic use, early gene expression patterns from both Epstein-Barr virus (EBV) and Raji cells were examined after inducing the lytic life cycle using 12-O-tetradecanoyl-13-phorbol ester and sodium butyrate. Real-time PCR identified several hig...

journal_title：Journal of medical microbiology

authors： Auburn H,Zuckerman M,Smith M

更新日期：2016-11-01 00:00:00

abstract：:A serial study of rotavirus infection in gnotobiotic piglets is described. They were infected when 7 days old and the course of infection was followed for 21 days, by immunofluorescence and histological examinations of the intestinal epithelium and by titration of the virus content of the gut lumen. There appeared to ...

journal_title：Journal of medical microbiology

authors： Crouch CF,Woode GN

更新日期：1978-08-01 00:00:00

abstract：:The object of this study was to develop and characterize experimental Burkholderia mallei aerosol infection in BALB/c mice. Sixty-five mice were infected with 5000 [approx. 2.5 median lethal doses (MLD)] B. mallei strain ATCC 23344(T) bacteria by the aerosol route. Bacterial counts within lung, liver, spleen, brain, k...

journal_title：Journal of medical microbiology

authors： Lever MS,Nelson M,Ireland PI,Stagg AJ,Beedham RJ,Hall GA,Knight G,Titball RW

更新日期：2003-12-01 00:00:00

abstract：:Meticillin-resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen in Pakistan and is emerging in the community. This is one of the first reports of the prevalent genotypes of MRSA in both hospital and community settings in Pakistan. Isolates collected in 2006-2007 were characterized by PFGE, staphylococ...

journal_title：Journal of medical microbiology

authors： Zafar A,Stone M,Ibrahim S,Parveen Z,Hasan Z,Khan E,Hasan R,Wain J,Bamford K

更新日期：2011-01-01 00:00:00

abstract：:Staphylococcus aureus strains were treated with ethylmethane sulphonate with the object of obtaining mutants that differed from the wild type in one of the properties that might contribute to virulence. Mutants with isolated loss or deficient production of gamma-haemolysin, coagulase, leucocidin and staphylokinase wer...

journal_title：Journal of medical microbiology

authors： van der Vijver JC,van Es-Boon MM,Michel MF

更新日期：1975-05-01 00:00:00

abstract：:The potential of human plasma (HP) or serum (HS) as a replacement for fetal calf serum (FCS) was evaluated in a liver infusion tryptose (LIT) medium for bulk cultivation of Leishmania donovani promastigotes. The promastigote yield with the LIT-FCS standard medium was 0.4-1.8×10(7) ml(-1), and yields of 0.5-3.4×10(7) (...

journal_title：Journal of medical microbiology

authors： Mahamoud A,Osman HA,Mansour D,El Harith A

更新日期：2013-08-01 00:00:00

abstract：:Clostridium difficile is a frequent cause of severe, recurrent, post-antibiotic diarrhoea and pseudomembranous colitis. Its pathogenicity is mediated mainly by two toxins, TcdA and TcdB. However, different adhesins have also been described as important colonization factors which are implicated in the first step of the...

journal_title：Journal of medical microbiology

authors： Barketi-Klai A,Hoys S,Lambert-Bordes S,Collignon A,Kansau I

更新日期：2011-08-01 00:00:00

abstract：:Cutaneous leishmaniasis (CL), a neglected tropical disease, is reported to be prevalent in tribal villages located in the Agasthyamala Biosphere Reserve forests of Western Ghats, Kerala state, India. We carried out an investigation to characterize the species of Leishmania parasites involved in these infections preval...

journal_title：Journal of medical microbiology

authors： Kumar NP,Srinivasan R,Anish TS,Nandakumar G,Jambulingam P

更新日期：2015-02-01 00:00:00

abstract：:Clinical and bacteriological findings in seven cases of urinary tract infection with cysteine-requiring strains of Klebsiella are described. The organisms were isolated from patients with long-standing urinary tract abnormalities and grew as small (c. 1 mm) colonies on MacConkey agar. The organisms failed to grow in a...

journal_title：Journal of medical microbiology

authors： McIver CJ,Tapsall JW

更新日期：1988-07-01 00:00:00

abstract：:The adhesion of bovine and a human isolate of Escherichia coli to epithelial cells from the teat and lactiferous sinuses of the udder was examined. Adhesion was detected with bacterial suspensions that produced mannose-sensitive agglutination of guinea-pig red cells. Adhesion to epithelial cells could be inhibited by ...

journal_title：Journal of medical microbiology

authors： Harper M,Turvey A,Bramley AJ

更新日期：1978-05-01 00:00:00

abstract：:Cholera toxin (CT) is the major virulence factor produced by Vibrio cholerae. Several genomic arrangements within the CTX cassette have been elucidated in V. cholerae. Previously, it was shown that three different CTX cassette arrangements, one complete CTX cassette (arrangement A), one complete and two incomplete CTX...

journal_title：Journal of medical microbiology

authors： Amin Marashi SM,Bakhshi B,Imani Fooladi AA,Tavakoli A,Sharifnia A,Pourshafie MR

更新日期：2012-08-01 00:00:00

abstract：:A cosmid DNA library had been constructed previously from 40-kb fragments of genomic DNA from a virulent invasive strain of Salmonella enterica serotype Typhimurium (TML) in an avirulent hypo-invasive Typhimurium strain (LT7). Selection of invasive clones from the library was attempted by iterative passage through a r...

journal_title：Journal of medical microbiology

authors： Robey M,Morgan E,Lodge JM,Bolton AJ,Martin GD,Brown NL,Stephen J

更新日期：2002-03-01 00:00:00

abstract：:Entero-invasive Escherichia coli (EIEC) and shigellae were tested for contact-haemolysin (CH) with red blood cells (RBCs) of guinea-pig, rabbit, rat, mouse, monkey, man, sheep and chicken; all bacteria showed the best lysis with guinea-pig RBCs. The best culture medium for CH activity of shigellae was tryptic soy brot...

journal_title：Journal of medical microbiology

authors： Haider K,Albert MJ,Hossain A,Nahar S

更新日期：1991-12-01 00:00:00

abstract：:The increasing incidence of reported scabies in Trinidad, from 24.2/100,000 population in 1984 to 59.5/100,000 in 1985, led to a careful monitoring in 1986 of all patients with post-streptococcal acute glomerulonephritis (PSAGN). There were 181 cases of PSAGN; 84 beta-haemolytic streptococcal isolates from 72 patients...

journal_title：Journal of medical microbiology

authors： Reid HF,Bassett DC,Gaworzewska E,Colman G,Poon-King T

更新日期：1990-06-01 00:00:00

abstract：:Prior to the advent of the Haemophilus influenzae type b vaccine, invasive infections due to H. influenzae type f were rarely described. However, the epidemiology of H. influenzae is changing. While the incidence of invasive infections due to H. influenzae is declining in children, such infections are becoming more co...

journal_title：Journal of medical microbiology

authors： Suarez CJ,Glover WA,Cowan J,Smith A,Clarridge JE

更新日期：2013-04-01 00:00:00

abstract：:Resistance patterns and carbapenemase gene presence among Klebsiella pneumoniae isolates from the University General Hospital of Patras, Greece during a ten-year period were analysed under a surveillance programme for multi-drug-resistant bacteria. From 2005 to 2014, K. pneumoniae isolates from clinically significant ...

journal_title：Journal of medical microbiology

authors： Spyropoulou A,Papadimitriou-Olivgeris M,Bartzavali C,Vamvakopoulou S,Marangos M,Spiliopoulou I,Anastassiou ED,Christofidou M

更新日期：2016-03-01 00:00:00

abstract：:To clarify further the role of chemotaxis in Helicobacter pylori colonization, the in vitro bacterium response to human plasma and bile (secretions containing chemoeffector compounds that are present in the gastric mucus layer) was examined. Human plasma, after dilution to 1 % (v/v) with buffer, was found to be a chem...

journal_title：Journal of medical microbiology

authors： Worku ML,Karim QN,Spencer J,Sidebotham RL

更新日期：2004-08-01 00:00:00

abstract：:Staphylococcus aureus is a leading aetiologic agent of nosocomial- and community-acquired infectious diseases worldwide. The public health concern regarding staphylococcal infections is inflated by the increasing occurrence of multidrug-resistant strains, e.g. multidrug- and meticillin-resistant S.aureus (MDR MRSA). T...

journal_title：Journal of medical microbiology

authors： Sarkar P,Acharyya S,Banerjee A,Patra A,Thankamani K,Koley H,Bag PK

更新日期：2016-10-01 00:00:00

abstract：:Two chlamydial enzyme immunoassays, Chlamydiazyme and IDEIA, were evaluated in a mouse model of chlamydial genital-tract infection. The Chlamydiazyme assay and the IDEIA were assessed on specimens from 10 and 11 mice, respectively. The animals were infected with Chlamydia trachomatis, strain SA2f, and the results obta...

journal_title：Journal of medical microbiology

authors： Taylor-Robinson D,Tuffrey M

更新日期：1987-09-01 00:00:00

abstract：:Campylobacter-like organisms, isolated from the gastric antrum of Rhesus monkeys, were compared with Campylobacter jejuni and C. pylori. They were similar to C. pylori by light microscopy, in ultrastructural morphology, in enzymic, fatty-acid-methyl-ester, and protein-profile analysis, and in antigenic reactivity with...

journal_title：Journal of medical microbiology

authors： Newell DG,Hudson MJ,Baskerville A

更新日期：1988-09-01 00:00:00