Abstract:
:Entero-invasive Escherichia coli (EIEC) and shigellae were tested for contact-haemolysin (CH) with red blood cells (RBCs) of guinea-pig, rabbit, rat, mouse, monkey, man, sheep and chicken; all bacteria showed the best lysis with guinea-pig RBCs. The best culture medium for CH activity of shigellae was tryptic soy broth, and for EIEC it was casamino acid-yeast extract broth with 1 mM CaCl2. CH production by all species was best at the slightly alkaline pH which is optimal for growth; it was also dependent on the presence of a large (140-Mda) plasmid. Pre-treatment of bacteria with homologous antisera inhibited CH activity. Various treatments of bacterial cells and RBCs suggested that CH may be a protein molecule, and that a chitotriose-like moiety may serve as CH receptor. RBCs that were incubated with bacteria at 4 degrees C, or with heat-killed bacteria at 37 degrees C, were not lysed; also, isolated cell-surface components (lipopolysaccharide and outer-membrane protein) did not lyse RBCs. This suggests that metabolically active cells are required for CH activity. Production of CH by both EIEC and shigellae is consistent with a common mechanism for the virulence of these organisms.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Haider K,Albert MJ,Hossain A,Nahar Sdoi
10.1099/00222615-35-6-330subject
Has Abstractpub_date
1991-12-01 00:00:00pages
330-7issue
6eissn
0022-2615issn
1473-5644journal_volume
35pub_type
杂志文章abstract::Streptolysin O (SLO) is a potent cytolytic toxin produced by almost all strains of group A streptococci and is considered an important virulence factor for this organism. In this study we investigated the effect of allicin and aqueous garlic extracts on the haemolytic activity of SLO. All tested materials potentially ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.019539-0
更新日期:2010-09-01 00:00:00
abstract::A novel aminopeptidase was purified by high performance liquid chromatography from a cytosoluble 100,000 g extract of Candida albicans on the basis of its ability to cleave L-arginine 7-amino-4-methylcoumarin. The purification factor was 36 and the yield was 20%. The native enzyme had a mol. wt of 52 kDa as demonstrat...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-43-4-282
更新日期:1995-10-01 00:00:00
abstract::Streptococcus pneumoniae remains a leading cause of serious paediatric disease. However, there are few published epidemiological data regarding invasive pneumococcal disease (IPD) in many countries in South East Asia, including Singapore. Baseline data for IPD are essential to inform policy regarding pneumococcal conj...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.030007-0
更新日期:2011-06-01 00:00:00
abstract::Sequence variations located at the signal sequence and mid-region within the vacA gene, the 3'-end of the cagA gene, the indel motifs at the 3'-end of the cag pathogenicity island and the regions upstream of the vacA and ribA genes were determined by PCR in 19 paired antral or antrum and corpus Helicobacter pylori iso...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.05440-0
更新日期:2004-07-01 00:00:00
abstract::Unlike their parent strains, zidovudine-resistant derivatives of Escherichia coli KL16 and Salmonella typhimurium NCTC 5710 were found to be incapable of incorporating radiolabelled thymidine into their chromosomal DNA. Since incorporation was still prevented in the presence of EDTA, resistance to zidovudine was not a...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-33-4-235
更新日期:1990-12-01 00:00:00
abstract::Fifty-eight isolates of Shigella sonnei from three outbreaks in school children and eight control isolates from epidemiologically unrelated sporadic clinical infections in Taiwan were compared by antibiotic susceptibility testing and molecular typing. Antibiotic susceptibility testing showed that all strains except on...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/0022-1317-49-4-355
更新日期:2000-04-01 00:00:00
abstract::An earlier study reported that human cerebrospinal fluid (CSF) has fungistatic activity for Cryptococcus neoformans. The present study reports that molecular sieve fractionation of concentrated CSF yielded three protein peaks, one of which (p2) had anticryptococcal activity. On a DEAE-Sephacel anion-exchange column th...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/0022-1317-50-1-83
更新日期:2001-01-01 00:00:00
abstract::Subtractive hybridisation was used to screen for and identify conserved DNA sequences associated with clinical, clonal populations of Staphylococcus aureus. DNA from S. aureus strain ISP8 (a clinical isolate) was digested with TaqI and hybridised with randomly fragmented pooled DNA from 10 non-clinical (community) iso...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-46-12-987
更新日期:1997-12-01 00:00:00
abstract::A total of 2401 isolates of Haemophilus parainfluenzae was isolated from respiratory secretions of 36 healthy adults and 128 patients with chronic bronchitis over a period of 1 year. The isolates were allocated to eight biotypes, by their production of indole, urease and ornithine decarboxylase. Biotypes I and II cons...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-36-4-279
更新日期:1992-04-01 00:00:00
abstract::The results of this study demonstrate that saline extracted surface-associated material (SAM) of Actinobacillus actinomycetemcomitans and Eikenella corrodens stimulates bone resorption at picomolar concentrations. Various inhibitors of known osteolytic mediators--indomethacin, interleukin-1 receptor antagonist (IL-1ra...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-41-3-197
更新日期:1994-09-01 00:00:00
abstract::Listeria monocytogenes cells are ingested by protozoa such as Acanthamoeba sp. or Tetrahymena pyriformis. However, they are not killed, but survive within the protozoa and may multiply intracellularly. The protozoa are lysed within about 8 days, releasing viable L. monocytogenes. No co-existence was observed between L...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-33-1-51
更新日期:1990-09-01 00:00:00
abstract::Small round viruses (SRV) were isolated from the faeces of diarrhoeic calves from three farms. All three SRV preparations caused diarrhoea experimentally in gnotobiotic calves. Each preparation contained viral particles of two morphological types, "astrovirus-like" and "calicivirus-like", and from one preparation the ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-11-4-441
更新日期:1978-11-01 00:00:00
abstract::The enzymic activity of 29 Haemophilus ducreyi strains on 28 substrates is described. The results are compared with those of seven other authors. There is agreement only about the presence of alkaline phosphatase and arginine aminopeptidase and the lack of glycosidases. Possible reasons for the contradictions in the e...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-18-2-181
更新日期:1984-10-01 00:00:00
abstract::Klebsiella pneumoniae meningitis has recently become an increasingly common cause of central nervous system infection. The invasion of bacteria within the subarachnoid space stimulates the release of pro-inflammatory cytokines and chemokines, triggering a host immune response. The aim of the present study was to evalu...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.063289-0
更新日期:2014-01-01 00:00:00
abstract::The aim of this study was to examine changes in the systemic immune response during the incubation period and following the onset of clinical swine dysentery, including the recovery period. Ten healthy conventional pigs were inoculated with Brachyspira hyodysenteriae. Blood was sampled at pre-inoculation, at days 4 an...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46538-0
更新日期:2006-07-01 00:00:00
abstract::Pseudomonas aeruginosa is an important opportunistic bacterial pathogen. Despite its metabolic and virulence versatility, it has not been shown to infect articular joints, which are areas that are rarely infected with bacteria in general. We hypothesized that articular joints possess antimicrobial activity that limits...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.051227-0
更新日期:2013-04-01 00:00:00
abstract::Candida albicans, as an opportunistic pathogen, can cause superficial and life-threatening candidiasis in immunocompromised individuals. The formation of surface-associated biofilms and the appearance of drug resistance pose a significant challenge for clinical intervention. In this study, a total of 104 hospital-acqu...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.080200-0
更新日期:2015-01-01 00:00:00
abstract::Over the last 30 years, Serratia marcescens has become an important cause of nosocomial infection. There have been many reports concerning the identification, antibiotic susceptibility, pathogenicity, epidemiological investigations and typing of this organism. Accurate identification is important in defining outbreaks...
journal_title:Journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.1099/00222615-46-11-903
更新日期:1997-11-01 00:00:00
abstract::The objectives of the present study were to evaluate in vitro the production of antagonistic compounds against Gardnerella vaginalis by Lactobacillus strains isolated from women with or without bacterial vaginosis (BV), and to select one of the better Lactobacillus producers of such a substance to be tested in vivo us...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.041962-0
更新日期:2012-08-01 00:00:00
abstract::Mice were vaccinated with lipopolysaccharide (LPS) from Leptospira interrogans serovar pomona or hardjo, or with the polysaccharide (PS) fraction of the LPS, or with an immunoconjugate of PS and diphtheria toxoid (DT). Maximum agglutinin titres were found 6-10 weeks after vaccination with LPS or PS-DT conjugate; the l...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-33-3-199
更新日期:1990-11-01 00:00:00
abstract::Breakthrough contamination of tuberculosis (TB) cultures is a problem in that it allows the overgrowth of another bacterium present in the sputum specimen, which can potentially mask the presence of Mycobacterium tuberculosis. The aim of this study was to isolate and characterize the bacterial organisms responsible fo...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.030619-0
更新日期:2011-09-01 00:00:00
abstract::A total of 287 Acinetobacter isolates belonging to DNA groups 2 (A. baumannii) and 13TU was collected consecutively from 46 hospitals and typed by randomly amplified polymorphic DNA fingerprinting with primers DAF-4 and ERIC-2. With a similarity coefficient of >/=72% as a cut-off value, 37 clusters of genotypically si...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/0022-1317-51-12-1107
更新日期:2002-12-01 00:00:00
abstract::Acinetobacter baumannii, Acinetobacter nosocomialis and Acinetobacter pittii are clinically relevant members of the Acinetobacter calcoaceticus-A. baumannii (Acb) complex and important nosocomial pathogens. These three species are genetically closely related and phenotypically similar; however, they differ in their ep...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.071712-0
更新日期:2014-09-01 00:00:00
abstract::The potential of exocellular carbohydrate antigens of Staphylococcus epidermidis as markers of infection in bone was investigated by immunoblotting and enzyme-linked immunosorbent assay (ELISA). Exocellular antigens were prepared by gel filtration chromatography of concentrated brain heart infusion culture supernates....
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-44-5-355
更新日期:1996-05-01 00:00:00
abstract::Historically, enteropathogenic Escherichia coli (EPEC) are a well-known cause of outbreaks of infantile diarrhoea associated with morbidity and mortality in England. The aim of this study was to provide an update on the microbiology and epidemiology of strains of EPEC in England between 2010 and 2012. A wide range of ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.062380-0
更新日期:2013-10-01 00:00:00
abstract::A collection of 72 strains of catalase-negative gram-positive, -negative and -variable cocco-bacilli isolated from samples of vaginal discharge from women with non-specific vaginal infection was examined in an attempt to develop an identification system for Gardnerella vaginalis that could be used in a diagnostic labo...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-16-1-83
更新日期:1983-02-01 00:00:00
abstract::The increased prevalence of oral candidosis associated with HIV infection must be intrinsically related to immunological changes in the host, but might also involve alterations to the infecting strains of yeast. This study aimed to determine if strains of Candida albicans isolated from asymptomatic HIV-infected indivi...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-43-6-452
更新日期:1995-12-01 00:00:00
abstract::Salmonella enterica serovar Typhimurium (S. Typhimurium) is the commonest pathogen causing food-borne disease among humans and animals in Australia. A multiplex PCR-based reverse line blot (mPCR/RLB) system was developed to rapidly identify S. Typhimurium phage types and strains within them. The system comprised 32 bi...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.47766-0
更新日期:2008-07-01 00:00:00
abstract::The aim of this study was the analysis of the cytokine response in BALB/c mice infected with the highly virulent RH or the weakly virulent Beverley strains of Toxoplasma gondii. Analysis of cytokine messages showed increased expression of IL12, IFN-gamma and TNF-alpha, but not IL4 mRNAs in spleen cells after infection...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.04860-0
更新日期:2003-10-01 00:00:00
abstract::Intratracheal administration of purified Pseudomonas aeruginosa exoenzyme S elicited extensive, grossly observable damage in the rat lung within 2 h. Light and electronmicroscopy revealed injury and necrosis of bronchial epithelium, type I pneumocytes and capillary endothelial cells after 1 h; associated haemorrhage, ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-26-2-133
更新日期:1988-06-01 00:00:00