Alteration of pulmonary structure by Pseudomonas aeruginosa exoenzyme S.

Abstract:

:Intratracheal administration of purified Pseudomonas aeruginosa exoenzyme S elicited extensive, grossly observable damage in the rat lung within 2 h. Light and electronmicroscopy revealed injury and necrosis of bronchial epithelium, type I pneumocytes and capillary endothelial cells after 1 h; associated haemorrhage, fibrinous exudation and released type II cell lamellar bodies in alveolar lumina after 1-12 h; progressively increasing accumulations of polymorphonuclear leucocytes in the bronchi and alveoli and in alveolar septae (interstitial pneumonia) after 1-12 h; collapse of alveolar septal connective tissue and damage to pulmonary arterioles and venules. Treatment of monolayer cultures of bronchial fibroblasts with purified exoenzyme S elicited vacuolation of the cells with apparent membrane damage as revealed by light and electronmicroscopy. In-vivo production and activity of P. aeruginosa exoenzyme S may be an important pathogenicity determinant in the necrotising lung injury characteristic of P. aeruginosa pneumonia.

journal_name

J Med Microbiol

authors

Woods DE,Hwang WS,Shahrabadi MS,Que JU

doi

10.1099/00222615-26-2-133

subject

Has Abstract

pub_date

1988-06-01 00:00:00

pages

133-41

issue

2

eissn

0022-2615

issn

1473-5644

journal_volume

26

pub_type

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