The isolation and cloning of chromosomal DNA specific for a clonal population of Staphylococcus aureus by subtractive hybridisation.

abstract：:This study analysed the invasiveness of Listeria monocytogenes into enterocyte-like Caco-2 cells in which iron depletion was achieved by picolinic acid treatment. Both entry and intracellular multiplication varied depending on the endogenous iron content of bacterial and eukaryotic cells. The behaviour within enterocy...

journal_title：Journal of medical microbiology

authors： Conte MP,Longhi C,Petrone G,Polidoro M,Valenti P,Seganti L

更新日期：2000-08-01 00:00:00

abstract：:Breast-fed infants are less susceptible to gastroenteritis than bottle-fed infants. Antibodies against rotavirus, the major pathogen of infantile gastroenteritis, were sought in human sera, colostrum and milk specimens by immunofluorescence. An experimental murine-rotavirus model was established by infecting the secon...

journal_title：Journal of medical microbiology

authors： Schoub BD,Prozesky OW,Lecatsas G,Oosthuizen R

更新日期：1978-02-01 00:00:00

abstract：:Amplification and sequence analysis of the 16S rRNA gene can be applied to detect and identify bacteria in clinical samples. We examined 75 clinical samples (17 culture-positive, 58 culture-negative) prospectively by two different PCR protocols, amplifying either a single fragment (1343 bp) or two fragments (762/598 b...

journal_title：Journal of medical microbiology

authors： Jenkins C,Ling CL,Ciesielczuk HL,Lockwood J,Hopkins S,McHugh TD,Gillespie SH,Kibbler CC

更新日期：2012-04-01 00:00:00

abstract：:To elucidate whether Chlamydia trachomatis and C. pneumoniae infections occur to a significant extent in monocytes-macrophages, the human monocytic cell line, U-937, was infected with C. trachomatis L2 or C. pneumoniae TW-183. Chlamydial DNA and genus-specific antigens of the lipopolysaccharides (LPS) in epitopes of t...

journal_title：Journal of medical microbiology

authors： Numazaki K,Suzuki K,Chiba S

更新日期：1995-03-01 00:00:00

abstract：:In recent years, hospitals in southeastern Europe have faced dramatically high rates of antibiotic-resistant Acinetobacter baumannii. We analysed the evolution of resistance among clinical isolates of A. baumannii group obtained from nine tertiary hospitals throughout Greece over 6 years (2010-2015). Identification an...

journal_title：Journal of medical microbiology

authors： Dafopoulou K,Tsakris A,Pournaras S

更新日期：2018-04-01 00:00:00

abstract：:Resistance patterns and carbapenemase gene presence among Klebsiella pneumoniae isolates from the University General Hospital of Patras, Greece during a ten-year period were analysed under a surveillance programme for multi-drug-resistant bacteria. From 2005 to 2014, K. pneumoniae isolates from clinically significant ...

journal_title：Journal of medical microbiology

authors： Spyropoulou A,Papadimitriou-Olivgeris M,Bartzavali C,Vamvakopoulou S,Marangos M,Spiliopoulou I,Anastassiou ED,Christofidou M

更新日期：2016-03-01 00:00:00

abstract：:During a one-year period, 258 isolates of Campylobacter jejuni and C. coli were obtained from children with gastroenteritis or bacteraemia at the Red Cross Children's Hospital, Cape Town, South Africa. These isolates were biotyped by hippurate hydrolysis, H2S production and tolerance to 2,3,5-triphenyltetrazolium chlo...

journal_title：Journal of medical microbiology

authors： Lastovica AJ,Le Roux E,Congi RV,Penner JL

更新日期：1986-02-01 00:00:00

abstract：:Some strains of Helicobacter pylori are known to produce an extracellular cytotoxin that causes vacuolation in cultured mammalian cells. Screening for such strains makes use of HeLa cells which may not be sensitive enough to detect minimal changes. The aim of this study was to develop a more sensitive cell line. Vacuo...

journal_title：Journal of medical microbiology

authors： Wang XM,Kojima T,Satoh K,Taniguchi Y,Tokumaru K,Saifuku K,Seki M,Kihira K,Ido K,Uchida JY,Ohmori C,Takaoka T,Kimura K

更新日期：1997-08-01 00:00:00

abstract：:Rickettsia conorii is endemic in Greece, though only a few cases of infection have been published to date. The case of a 58-year-old man from northern Greece with a severe form of Mediterranean spotted fever and rapid neurological deterioration is presented here. The patient received antibiotic treatment with doxycycl...

journal_title：Journal of medical microbiology

authors： Tzavella K,Hatzizisis IS,Vakali A,Mandraveli K,Zioutas D,Alexiou-Daniel S

更新日期：2006-03-01 00:00:00

abstract：:The aim of this study was to evaluate the presence and prevalence of multidrug antibacterial resistance in Helicobacter pylori in Bulgaria from 2005 to 2008. The resistance in 828 untreated adults, 124 treated adults and 105 untreated children was, respectively, 26.5, 50.8 and 16.2% for metronidazole; 18.4, 45.2 and 1...

journal_title：Journal of medical microbiology

authors： Boyanova L

更新日期：2009-07-01 00:00:00

abstract：:A polymerase chain reaction (PCR) assay with oligonucleotide primers homologous to a portion of the urease C gene of Helicobacter pylori was evaluated for specificity with pure DNA and biopsy material. The assay was used to test for the presence of the organism in dental plaque. The species specificity of detection wa...

journal_title：Journal of medical microbiology

authors： Bickley J,Owen RJ,Fraser AG,Pounder RE

更新日期：1993-11-01 00:00:00

abstract：:This study investigated the comparative performance of the Amplicor assay and an in-house semi-automated, multiplex real-time PCR for the diagnosis of genital chlamydial infection. Four different assays, the COBAS Amplicor CT test (Amplicor PCR), in-house real-time PCR (IHRT-PCR), in-house nested cryptic plasmid PCR a...

journal_title：Journal of medical microbiology

authors： Jalal H,Al-Suwaine A,Stephen H,Carne C,Sonnex C

更新日期：2007-03-01 00:00:00

abstract：:The effect of glucan on Leishmania major infection was studied in BALB/c mice, which are highly susceptible to leishmania infection. Glucan (0.45 mg), or isovolumetric dextrose, was administered intraperitoneally 7, 5, 3 and 1 day before infection with L. major promastigotes. At 3, 5, 6, 8 and 10 weeks after infection...

journal_title：Journal of medical microbiology

authors： Al Tuwaijri AS,Mahmoud AA,Al Mofleh IA,Al Khuwaitir SA

更新日期：1987-06-01 00:00:00

abstract：:Sorbarod biofilms were investigated for their suitability in establishing continuous culture biofilms for the study of bacterial vaginosis. Two important organisms in the condition, Gardnerella vaginalis and Lactobacillus acidophilus, were studied. In contrast to growth in broth culture, both organisms were maintained...

journal_title：Journal of medical microbiology

authors： Muli FW,Struthers JK

更新日期：1998-05-01 00:00:00

abstract：:Erythrocyte-sensitising antigens (AE) were prepared from Vibrio cholerae serotypes, from EL-Tor vibrio, Escherichia coli and Salmonella enteritidis by digesting the organisms with NaOH followed by precipitation with alcohol. When AE was used in indirect haemagglutination (IHA) tests, the results in a number of cases w...

journal_title：Journal of medical microbiology

authors： Garabedian GA,Barsumian EL,Malakian A

更新日期：1975-02-01 00:00:00

abstract：:Historically, enteropathogenic Escherichia coli (EPEC) are a well-known cause of outbreaks of infantile diarrhoea associated with morbidity and mortality in England. The aim of this study was to provide an update on the microbiology and epidemiology of strains of EPEC in England between 2010 and 2012. A wide range of ...

journal_title：Journal of medical microbiology

authors： Sakkejha H,Byrne L,Lawson AJ,Jenkins C

更新日期：2013-10-01 00:00:00

abstract：:Members of the Burkholderia cepacia complex are important bacterial pathogens in cystic fibrosis (CF) patients. The B. cepacia complex currently consists of nine genetic subgroups (genomovars) of different epidemiological relevance and possibly of different pathogenic potential in humans. In this study, a new approach...

journal_title：Journal of medical microbiology

authors： Vonberg RP,Häußler S,Vandamme P,Steinmetz I

更新日期：2006-06-01 00:00:00

abstract：:The rat was evaluated as an experimental model for disseminated candidosis by quantitating blood clearance and initial organ localisation of 3H-leucine-labelled Candida albicans after intravenous injection into the tail or portal vein. Viable or formalin-killed blastoconidia or viable blastoconidia with germ tubes wer...

journal_title：Journal of medical microbiology

authors： Katz S,Grosfeld JL,Folkening WJ,Rosenthal RS,Merkel GJ

更新日期：1992-10-01 00:00:00

abstract：:In this study, we investigated the in vitro antifungal effects of itraconazole/voriconazole (ITR/VRC) alone and in combination with tetrandrine (TET) against 23 clinical isolates of A. fumigatus using a chequerboard microdilution method. The dynamic antifungal effects of TET with ITR/VRC against A. fumigatus were asse...

journal_title：Journal of medical microbiology

authors： Li SX,Song YJ,Zhang LL,Shi JP,Ma ZL,Guo H,Dong HY,Li YM,Zhang H

更新日期：2015-09-01 00:00:00

abstract：:The distribution of K1, K5 and O antigens was examined in 500 clinical strains of Escherichia coli. Of 400 strains from urine, 52% belonged to serogroups O1, O2, O4, O6, O7, O8, O9, O18, O25 and O75; 34% were non-typable (NT) and 14% were autoagglutinable (AA). Antigen K1 was carried by 17% of these strains, and K5 by...

journal_title：Journal of medical microbiology

authors： Devine DA,Robinson L,Roberts AP

更新日期：1989-12-01 00:00:00

abstract：:Streptococcus pneumoniae remains a leading cause of serious paediatric disease. However, there are few published epidemiological data regarding invasive pneumococcal disease (IPD) in many countries in South East Asia, including Singapore. Baseline data for IPD are essential to inform policy regarding pneumococcal conj...

journal_title：Journal of medical microbiology

authors： Jefferies JMC,Tee WSN,Clarke SC

更新日期：2011-06-01 00:00:00

abstract：:Cutaneous leishmaniasis (CL), a neglected tropical disease, is reported to be prevalent in tribal villages located in the Agasthyamala Biosphere Reserve forests of Western Ghats, Kerala state, India. We carried out an investigation to characterize the species of Leishmania parasites involved in these infections preval...

journal_title：Journal of medical microbiology

authors： Kumar NP,Srinivasan R,Anish TS,Nandakumar G,Jambulingam P

更新日期：2015-02-01 00:00:00

abstract：:Preliminary differentiation of Leptospira interrogans serovars hardjo and balcanica can be made with antisera that are collected from hamsters after experimental infection with serovar hardjo and selected for high homologous and low or nil heterologous titres, as measured by the microscopic agglutination test. Rabbit ...

journal_title：Journal of medical microbiology

authors： Ris DR,Hamel KL

更新日期：1978-08-01 00:00:00

abstract：:Two chlamydial enzyme immunoassays, Chlamydiazyme and IDEIA, were evaluated in a mouse model of chlamydial genital-tract infection. The Chlamydiazyme assay and the IDEIA were assessed on specimens from 10 and 11 mice, respectively. The animals were infected with Chlamydia trachomatis, strain SA2f, and the results obta...

journal_title：Journal of medical microbiology

authors： Taylor-Robinson D,Tuffrey M

更新日期：1987-09-01 00:00:00

abstract：:An affinity procedure with purified, biotinylated human transferrin and streptavidin-agarose was used to identify the transferrin-binding proteins in strains of Haemophilus influenzae. Proteins of 58 and 98 Kda were isolated from total membranes prepared from iron-deficient but not iron-sufficient H. influenzae KC548 ...

journal_title：Journal of medical microbiology

authors： Schryvers AB

更新日期：1989-06-01 00:00:00

abstract：:A collection of Enterococcus faecalis strains from clinical isolates, healthy individuals and the environment was screened for the presence of virulence factor genes, such as those for collagen-binding protein (ace), endocarditis antigen (efaA), haemolysin activator (cylA), gelatinase (gelE), aggregation substances (a...

journal_title：Journal of medical microbiology

authors： Creti R,Imperi M,Bertuccini L,Fabretti F,Orefici G,Di Rosa R,Baldassarri L

更新日期：2004-01-01 00:00:00

abstract：:The genetic characteristics of Vibrio parahaemolyticus strains isolated in 2004 and 2005 in Mozambique were assessed in this study to determine whether the pandemic clone of V. parahaemolyticus O3 : K6 and O4 : K68 serotypes has spread to Mozambique. Fifty-eight V. parahaemolyticus strains isolated from hospitalized d...

journal_title：Journal of medical microbiology

authors： Ansaruzzaman M,Chowdhury A,Bhuiyan NA,Sultana M,Safa A,Lucas M,von Seidlein L,Barreto A,Chaignat CL,Sack DA,Clemens JD,Nair GB,Choi SY,Jeon YS,Lee JH,Lee HR,Chun J,Kim DW

更新日期：2008-12-01 00:00:00

abstract：:The Pro-Pro-Glu (PPE) protein family of Mycobacterium tuberculosis includes 69 glycine-rich proteins with a conserved N-terminal domain. Their role in tuberculosis is unknown, but it has been speculated that they may have an important immunological significance. In this investigation, the immunogenicity of the ppe44 (...

journal_title：Journal of medical microbiology

authors： Bonanni D,Rindi L,Lari N,Garzelli C

更新日期：2005-05-01 00:00:00

abstract：:Brevundimonas diminuta is rarely associated with invasive infections. We report the case of a previously healthy young man with pleural effusion, in which B. diminuta was recovered but incorrectly identified as Kingella kingae when it was freshly isolated. Consequently, the misidentification resulted in initial treatm...

journal_title：Journal of medical microbiology

authors： Lu B,Shi Y,Zhu F,Xu X

更新日期：2013-03-01 00:00:00

abstract：:Terminal restriction fragment-length polymorphism (T-RFLP) analysis was applied to characterize oral bacterial flora in saliva from 18 healthy subjects and 18 patients with periodontitis. The 16S rRNA genes (rDNAs) of oral bacteria and spirochaetes in saliva were amplified by PCR with a 6'carboxy-fluorescein (6-FAM)-l...

journal_title：Journal of medical microbiology

authors： Sakamoto M,Takeuchi Y,Umeda M,Ishikawa I,Benno Y

更新日期：2003-01-01 00:00:00