Abstract：
:Two chlamydial enzyme immunoassays, Chlamydiazyme and IDEIA, were evaluated in a mouse model of chlamydial genital-tract infection. The Chlamydiazyme assay and the IDEIA were assessed on specimens from 10 and 11 mice, respectively. The animals were infected with Chlamydia trachomatis, strain SA2f, and the results obtained by these methods on vaginal specimens taken on 4 or 5 occasions during 41-42 days were compared with those obtained in cell culture and to a less extent by the MicroTrak direct immunofluorescence test. In comparison with culture, the Chlamydiazyme assay had a sensitivity of 62% and a specificity of 92%; IDEIA had a sensitivity of 76% and a specificity of 94%. These assays sometimes did not detect chlamydiae in specimens taken immediately before specimens which proved positive by culture and the immunoassays were less sensitive if swabs were taken after those for culture. The IDEIA also failed to detect chlamydiae in the late stage of the murine infection when chlamydial elementary bodies were seen by immunofluorescence. The implications of the observations for investigations in the human field as well as for further studies in the mouse are discussed.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Taylor-Robinson D,Tuffrey Mdoi
10.1099/00222615-24-2-169subject
Has Abstractpub_date
1987-09-01 00:00:00pages
169-73issue
2eissn
0022-2615issn
1473-5644journal_volume
24pub_type
杂志文章abstract：:Nearly 1000 sera from children were tested by immunoelectro-osmophoresis against BK virus, and age-specific prevalence rates were estimated from birth until the age of 12 years. Declining rates during the first 12 months showed the waning of passive immunity, which at birth reflects the mother's immune status. The cha...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-15-3-285
更新日期：1982-08-01 00:00:00
abstract：PURPOSE:Platelet concentrates are at risk of transfusion-related sepsis. The microbial detection methods currently available have reached their limits, as they do not completely prevent transfusion-related bacterial contamination.The aim of this study was to develop a new strategy to detect the risk of platelet transfu...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000533
更新日期：2017-11-01 00:00:00
abstract：:The heparin-binding properties of six different species of coagulase-negative staphylococci were examined by a particle agglutination assay. Heparin (mol. wt 4000-6000), mildly treated with sodium periodate, was covalently coupled to amino-modified latex beads (0.72 micron diameter). The particle agglutination assay w...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-45-4-263
更新日期：1996-10-01 00:00:00
abstract：:The growth of 29 strains of Acinetobacter spp. on semi-solid media was studied; 19 showed surface swarming and 14 produced channels ("ditches") in the agar that do not seem to have been described previously. An attempt was made to define the cultural and physical conditions for the demonstration of these phenomena. Po...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-8-3-443
更新日期：1975-08-01 00:00:00
abstract：:A total of 1878 non-duplicate clinical Escherichia coli isolates (comprising 1711 urinary isolates and 167 blood-culture isolates), which were collected from multiple centres in Hong Kong during 1996-2008, were used to investigate the prevalence and molecular epidemiology of plasmid-mediated fosfomycin (fos) resistanc...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.062653-0
更新日期：2013-11-01 00:00:00
abstract：:We present a case of septicaemia caused by Arcanobacterium haemolyticum in a previously healthy 23-year-old man suffering from acute pharyngotonsillitis, who developed complicated Lemierre's syndrome. Three blood cultures (both aerobic and anaerobic) revealed the exclusive presence of A. haemolyticum. The presence of ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.007492-0
更新日期：2009-12-01 00:00:00
abstract：PURPOSE:Extensively drug-resistant (XDR) strains of Acinetobacter baumannii are being reported worldwide, and they are associated with high morbidity and mortality rates. These strains are considered to be the highest priority for the development of new antibacterial agents. Therefore, we aimed to develop an effective ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.001002
更新日期：2019-07-01 00:00:00
abstract：:Aminoglycoside-resistant variants of Pseudomonas aeruginosa strain PAO1 were readily selected by culturing the organism in medium containing increasing concentrations of gentamicin, tobramycin or amikacin until the strains were growing in a concentration of drug 128-fold greater than the minimal inhibitory concentrati...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-29-1-41
更新日期：1989-05-01 00:00:00
abstract：:From 1997 to 2006, in the province of Quebec, Canada, 15 isolates of Streptococcus pseudoporcinus from 1 urine and 14 vaginorectal cultures were recovered from the genitourinary tract of pregnant women. All these women originated from the Caribbean or sub-Saharan Africa (P=0.00045 compared with a suitable control grou...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.47295-0
更新日期：2007-12-01 00:00:00
abstract：:Subtractive hybridisation was used to screen for and identify conserved DNA sequences associated with clinical, clonal populations of Staphylococcus aureus. DNA from S. aureus strain ISP8 (a clinical isolate) was digested with TaqI and hybridised with randomly fragmented pooled DNA from 10 non-clinical (community) iso...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-46-12-987
更新日期：1997-12-01 00:00:00
abstract：:The purpose of this study was to compare the frequency of recovery of aerobic and anaerobic organisms with interfering capability against group A beta-haemolytic streptococci (GABHS) and beta-lactamase-producing bacteria (BLPB) from the tonsils of GABHS carriers and non-carriers. The presence of aerobic and anaerobic ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.46796-0
更新日期：2006-12-01 00:00:00
abstract：:The production of toxins A and B by Clostridium difficile was greatly enhanced under biotin-limited conditions, in which a 140-kDa protein was expressed strongly. Gene cloning revealed that this protein was a homologue of formylglycinamidine ribonucleotide synthetase (FGAM synthetase, EC 6.3.5.3), which is known as Pu...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/0022-1317-51-1-34
更新日期：2002-01-01 00:00:00
abstract：:Clostridium difficile is the most common cause of nosocomial bacterial diarrhoea in the Western world. Diarrhoea and colitis are caused by the actions of toxins A and B released by pathogenic strains of C. difficile. Adaptive immune responses to these toxins influence the outcomes of C. difficile infection (CDI). Symp...
journal_title：Journal of medical microbiology
pub_type： 杂志文章,评审
doi：10.1099/jmm.0.030015-0
更新日期：2011-08-01 00:00:00
abstract：:There is a paucity of information about the factors associated with oral colonization with Candida species and the changes associated with antiretroviral (ARV) therapy. This study investigated the role of ARV therapy and other factors in a study population. Relevant clinical and laboratory information was obtained and...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.047522-0
更新日期：2013-01-01 00:00:00
abstract：:Terminal restriction fragment-length polymorphism (T-RFLP) analysis was applied to characterize oral bacterial flora in saliva from 18 healthy subjects and 18 patients with periodontitis. The 16S rRNA genes (rDNAs) of oral bacteria and spirochaetes in saliva were amplified by PCR with a 6'carboxy-fluorescein (6-FAM)-l...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.04991-0
更新日期：2003-01-01 00:00:00
abstract：:The activities of three sulphonamides and trimethoprim against strains of Pseudomonas aeruginosa have been studied. Sulphadiazine had most activity, sulphadimidine had little, and the activity of sulphamethoxazole was intermediate. According to their sensitivity to sulphamethoxazole, strains were divided into two grou...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-10-3-273
更新日期：1977-08-01 00:00:00
abstract：:Intravenous injection of eight human strains of Campylobacter fetus ss fetus and Campylobacter jejuni into mice at various stages of pregnancy demonstrated significant strain differences in ability to affect implantation of the fertilised ovum and to cause resorption of the mouse fetus. Implantation was significantly ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-25-1-7
更新日期：1988-01-01 00:00:00
abstract：:In recent years, hospitals in southeastern Europe have faced dramatically high rates of antibiotic-resistant Acinetobacter baumannii. We analysed the evolution of resistance among clinical isolates of A. baumannii group obtained from nine tertiary hospitals throughout Greece over 6 years (2010-2015). Identification an...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000708
更新日期：2018-04-01 00:00:00
abstract：:Campylobacter jejuni is a frequent cause of infectious diarrhoea and is increasingly recognized as a trigger for late-onset complications. The poor standardization of commonly used serological tests might explain the conflicting results regarding the frequency of antecedent C. jejuni infections in defined patient grou...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.46040-0
更新日期：2005-08-01 00:00:00
abstract：:A novel aminopeptidase was purified by high performance liquid chromatography from a cytosoluble 100,000 g extract of Candida albicans on the basis of its ability to cleave L-arginine 7-amino-4-methylcoumarin. The purification factor was 36 and the yield was 20%. The native enzyme had a mol. wt of 52 kDa as demonstrat...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-43-4-282
更新日期：1995-10-01 00:00:00
abstract：:Introduction.Mycoplasma genitalium is a sexually transmitted pathogen with increasing resistance to first- and second-line antimicrobials. The 'near-patient test' ResistancePlus MG FleXible (SpeeDx) detects M. genitalium plus four macrolide resistance mutations (MRMs), facilitating same-day patient follow up.Hypothesi...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.001271
更新日期：2021-01-01 00:00:00
abstract：:This study investigated the influence of the carbon source of the growth medium, strains of Candida albicans and source of epithelial cells, and the influence of smoking and gender, on the adhesion of C. albicans to epithelial cells from insulin-using diabetic patients. Adhesion was determined by an autologous adhesio...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/0022-1317-49-3-291
更新日期：2000-03-01 00:00:00
abstract：:Catalase (KatA) is known to play an important role in oxidative stress resistance in many bacterial species and a homologue exists in Helicobacter hepaticus, a member of the enterohepatic Helicobacter species. Here, a katA mutant was constructed by insertional mutagenesis and its oxidative stress phenotype was investi...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.46891-0
更新日期：2007-04-01 00:00:00
abstract：:Laribacter hongkongensis is a food-borne bacterium associated with community-acquired gastroenteritis and diarrhoea. Quinolone resistance was recently reported in bacterial isolates from aquatic products, but the molecular mechanisms for resistance were still unknown. In this study, a total of 157 L. hongkongensis str...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.059451-0
更新日期：2013-10-01 00:00:00
abstract：:Shanson's medium, a MacConkey-type agar containing added inhibitors, was compared with deoxycholate-citrate agar for the examination of faeces. The isolation rate of samonellae on the two media was similar, but Shanson's medium was the more selective; this meant that the amount of work required to produce a result was...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-8-2-365
更新日期：1975-05-01 00:00:00
abstract：:SDS-PAGE and iso-enzyme analysis of 11 human isolates of Blastocystis hominis revealed at least two variants with different polypeptide patterns and two zymodemes, respectively. This is the first iso-enzyme and the second protein analysis to indicate strain differences in B. hominis. ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-42-4-304
更新日期：1995-04-01 00:00:00
abstract：:Acinetobacter baumannii is an emerging multidrug-resistant pathogen and very little information is available regarding its imipenem resistance in Latin American countries such as Bolivia. This study investigated the antimicrobial resistance profile of 46 clinical strains from different hospitals in Cochabamba, Bolivia...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.032722-0
更新日期：2012-01-01 00:00:00
abstract：:Gardnerella vaginalis is a Gram-variable anaerobic bacterium present in 100% of women with bacterial vaginosis (BV). BV is a complex polymicrobial condition with no single causative agent. The current laboratory detection method for BV relies on a Gram-stain Nugent score to estimate the quantity of different bacterial...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000118
更新日期：2015-09-01 00:00:00
abstract：:A total of 287 Acinetobacter isolates belonging to DNA groups 2 (A. baumannii) and 13TU was collected consecutively from 46 hospitals and typed by randomly amplified polymorphic DNA fingerprinting with primers DAF-4 and ERIC-2. With a similarity coefficient of >/=72% as a cut-off value, 37 clusters of genotypically si...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/0022-1317-51-12-1107
更新日期：2002-12-01 00:00:00
abstract：PURPOSE:Diabetic patients seem to be predisposed to cutaneous candidiasis. In this study, we evaluated the interference of diabetic conditions in alloxan-induced diabetic mice in relation to the development of C. albicans infection, density of M1 and M2 macrophages, distribution of collagen type I and III and anti-infl...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000496
更新日期：2017-06-01 00:00:00
