Detection and identification of bacteria in clinical samples by 16S rRNA gene sequencing: comparison of two different approaches in clinical practice.

abstract：:Globally, specific genotypes of Mycobacterium tuberculosis have been shown to dominate in patients, suggesting that these are more successful pathogens. One such genotype, the F15/LAM4/KZN (KZN) family of M. tuberculosis, has predominated in KwaZulu-Natal, South Africa, since the early 1990s. This strain recently evol...

journal_title：Journal of medical microbiology

authors： Ashiru OT,Pillay M,Sturm AW

更新日期：2010-05-01 00:00:00

abstract：:Clinical isolates of Neisseria gonorrhoeae harbouring resistance (R) plasmids of mol. wts 4.4 x 10(6) (Asian) or 3.2 x 10(6) (African) were grown in prolonged glucose-limited continuous culture to determine the segregation efficiency of each type of plasmid and their expression of penicillinase activity in the absence...

journal_title：Journal of medical microbiology

authors： Keevil CW,Spillane BJ,Major NC

更新日期：1987-12-01 00:00:00

abstract：:We report a case of septicaemia with prolonged, refractory hypotension related to Moraxella osloensis isolated in a non-immunocompromised paediatric patient. ...

journal_title：Journal of medical microbiology

authors： Dien Bard J,Lewinski M,Summanen PH,Deville JG

更新日期：2011-01-01 00:00:00

abstract：:The activities of three sulphonamides and trimethoprim against strains of Pseudomonas aeruginosa have been studied. Sulphadiazine had most activity, sulphadimidine had little, and the activity of sulphamethoxazole was intermediate. According to their sensitivity to sulphamethoxazole, strains were divided into two grou...

journal_title：Journal of medical microbiology

authors： Grey D,Hamilton-Miller JM

更新日期：1977-08-01 00:00:00

abstract：:The opsonic requirements of Helicobacter pylori were investigated in a series of experiments with human polymorphonuclear leucocytes (PMNL). Pre-incubation of H. pylori with pooled normal human serum (NHS) in concentrations of 5-20% significantly increased the uptake of radiolabelled bacteria by PMNL. Treatment of the...

journal_title：Journal of medical microbiology

authors： McKinlay AW,Young A,Russell RI,Gemmell CG

更新日期：1993-03-01 00:00:00

abstract：:Thirty-seven multidrug-resistant and 13 pan-susceptible isolates of Mycobacterium tuberculosis were analysed for the diversity of genotypes associated with known drug-resistance mechanisms. The isolates were obtained from patients attending a university tuberculosis clinic in Monterrey, Mexico. A total of 25 IS6110-RF...

journal_title：Journal of medical microbiology

authors： Ramaswamy SV,Dou SJ,Rendon A,Yang Z,Cave MD,Graviss EA

更新日期：2004-02-01 00:00:00

abstract：:The distribution of hepatitis C virus (HCV) genotypes among Egyptian patients positive for anti-HCV was determined and their influence, when combined with neu-oncoprotein overexpression, on the development of hepatocellular carcinoma (HCC) was examined. The study groups included asymptomatic carriers (ASC) and patient...

journal_title：Journal of medical microbiology

authors： Zekri AN,Bahnassy AA,Shaarawy SM,Mansour OA,Maduar MA,Khaled HM,El-Ahmadi O

更新日期：2000-01-01 00:00:00

abstract：:Chlamydia trachomatis results in tubal factor infertility in some women. Diagnosis of this tubal infertility is difficult and typically involves laparoscopy or hysterosalpingography to detect the tubal blockages. Numerous serological tests have been developed; however, they are presently not used for diagnosis without...

journal_title：Journal of medical microbiology

authors： Menon S,Stansfield SH,Logan B,Hocking JS,Timms P,Rombauts L,Allan JA,Huston WM

更新日期：2016-09-01 00:00:00

abstract：PURPOSE:The aim of this study was to assess the biotope of the Cryptococcus neoformans/Cryptococcus gattii species complex from Ivory Coast, and clarify the possible epidemiological relationship between environmental and clinical strains. METHODOLOGY:Samples from Eucalyptus camaldulensis (n=136), Mangifera indica (n=1...

journal_title：Journal of medical microbiology

authors： Kassi FK,Bellet V,Drakulovski P,Krasteva D,Roger F,Valérie BA,Aboubakar T,Doumbia A,Kouakou GA,Delaporte E,Reynes J,Yavo W,Menan HIE,Bertout S

更新日期：2018-01-01 00:00:00

abstract：:Helicobacter pylori is believed to trigger neutrophil activation through several factors, including the H. pylori neutrophil-activating protein (HpNAP). The aim of this study was to characterise the factors within H. pylori cell-free extracts that stimulate neutrophil activation. Neutrophil activation was found to be ...

journal_title：Journal of medical microbiology

authors： Leakey A,Hirst R,LA Brooy J

更新日期：2001-09-01 00:00:00

abstract：:Chemotaxis of polymorphonuclear leukocytes (PMNL) in response to cell components of Bacteroides fragilis alone or in combination with Escherichia coli was evaluated. E. coli produced much more powerful chemotactic factors than B. fragilis. The culture filtrate (CF), outer membrane (OM) preparation, and lipopolysacchar...

journal_title：Journal of medical microbiology

authors： Namavar F,Verweij-Van Vught AM,Bal M,MacLaren DM

更新日期：1987-09-01 00:00:00

abstract：:During a one-year period, 258 isolates of Campylobacter jejuni and C. coli were obtained from children with gastroenteritis or bacteraemia at the Red Cross Children's Hospital, Cape Town, South Africa. These isolates were biotyped by hippurate hydrolysis, H2S production and tolerance to 2,3,5-triphenyltetrazolium chlo...

journal_title：Journal of medical microbiology

authors： Lastovica AJ,Le Roux E,Congi RV,Penner JL

更新日期：1986-02-01 00:00:00

abstract：:During the first quarter of 1996, a major outbreak of clinical infection caused by multiresistant Klebsiella pneumoniae (MRKP) occurred in the neonatal ward of the 'Maternité Wassila Bourguiba' in Tunis, Tunisia. In total, 32 isolates of MRKP, comprising 23 clinical isolates and nine surveillance isolates, were recove...

journal_title：Journal of medical microbiology

authors： Ben-Hamouda T,Foulon T,Ben-Cheikh-Masmoudi A,Fendri C,Belhadj O,Ben-Mahrez K

更新日期：2003-05-01 00:00:00

abstract：:Four hundred and twenty-seven Escherichia coli isolates from 427 cases of infantile diarrhoea in Poland, belonging to serogroups O18, O26, O44, O86, O126 and O127 and 150 E. coli isolates from 52 healthy children were examined for selected virulence properties. The presence of the plasmid pAA, a plasmid encoding enter...

journal_title：Journal of medical microbiology

authors： Paciorek J

更新日期：2002-07-01 00:00:00

abstract：:We report a third case of Mycobacterium conceptionense infection, which was found in a 50-year-old female following face rejuvenation surgery with fat grafting. The pathogen was identified using 16S rRNA gene and rpoB gene sequences. The growing diversity of non-tuberculosis mycobacterial species causing human infecti...

journal_title：Journal of medical microbiology

authors： Yang HJ,Yim HW,Lee MY,Ko KS,Yoon HJ

更新日期：2011-03-01 00:00:00

abstract：:Most past studies of cross-infection with Pseudomonas aeruginosa among cystic fibrosis (CF) patients in the UK suggest that it is a rare occurrence. However, two recent reports of highly transmissible strains in patients in regional centres in England (Liverpool and Manchester) have raised questions as to the extent o...

journal_title：Journal of medical microbiology

authors： Scott FW,Pitt TL

更新日期：2004-07-01 00:00:00

abstract：:The effects of ethyleneglycoltetra-acetic acid (EGTA) and EGTA + magnesium (MgEGTA) on the viable counts of 10 strains of Escherichia coli O6 have been studied in normal human serum (NHS), heat-inactivated serum (HIS) and in culture media with and without the addition of a beta-lactam antibiotic. The addition of EGTA ...

journal_title：Journal of medical microbiology

authors： Roberts AP,Phillips R

更新日期：1981-05-01 00:00:00

abstract：:Small numbers of bacteria capable of growing on agar supplemented with amoxycillin 40 mg/L were isolated from the saliva of 9 out of 20 adult volunteers in a previous study. All the bacteria were identified as Streptococcus sanguis although no strains produced dextran in conventional tests. However, using a specific a...

journal_title：Journal of medical microbiology

authors： Marsh PD,West AA,Keevil CW

更新日期：1987-11-01 00:00:00

abstract：:The genetic diversity between major meticillin-resistant Staphylococcus aureus (MRSA) lineages was probed using fluorescent amplified fragment length polymorphism (FAFLP) as a random genome sampling tool. Genomic DNA was digested with endonucleases BglII and Csp6I and a subset of the restricted fragments were amplifie...

journal_title：Journal of medical microbiology

authors： Desai D,Ellington MJ,Arnold C,Desai M

更新日期：2012-12-01 00:00:00

abstract：:The substrates inositol, rhamnose, d-tartrate and m-tartrate used in fermentation tests with 338 cultures of Salmonella paratyphi B differentiated strains in some phage types to give information that could be used in epidemiological investigations. Xylose in Bitter's medium, the fifth substrate by which 13 of a potent...

journal_title：Journal of medical microbiology

authors： Barker RM,Kearney GM,Nicholson P,Blair AL,Porter RC,Crichton PB

更新日期：1988-08-01 00:00:00

abstract：:Pseudomonas aeruginosa is an opportunistic human pathogen that causes serious and chronic infections. Many secondary metabolites are secreted throughout its growth, among which phenazine is a known virulence factor and signalling molecule. Phenazine is coordinately controlled by the global regulatory quorum-sensing (Q...

journal_title：Journal of medical microbiology

authors： Liang H,Duan J,Sibley CD,Surette MG,Duan K

更新日期：2011-01-01 00:00:00

abstract：:Human granulocytic anaplasmosis (HGA) and Lyme borreliosis (LB) are tick-borne infectious diseases caused by Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato species, respectively. In this study, p44/msp2 paralogues specific to A. phagocytophilum and 5S-23S rRNA gene-intergenic spacers specific to B. burg...

journal_title：Journal of medical microbiology

authors： Masuzawa T,Kharitonenkov IG,Okamoto Y,Fukui T,Ohashi N

更新日期：2008-08-01 00:00:00

abstract：:The nucleolar accumulation of Semliki Forest Virus (SFV) C protein was examined as a function of intact microtubules, intact microfilaments and accessible intermediate filaments. The cytoskeletal components do not seem to play a role in directing C protein to the nucleolus but nucleolar accumulation is energy-dependen...

journal_title：Journal of medical microbiology

authors： Jakob R

更新日期：1994-06-01 00:00:00

abstract：PURPOSE:Diabetic patients seem to be predisposed to cutaneous candidiasis. In this study, we evaluated the interference of diabetic conditions in alloxan-induced diabetic mice in relation to the development of C. albicans infection, density of M1 and M2 macrophages, distribution of collagen type I and III and anti-infl...

journal_title：Journal of medical microbiology

authors： Pupim ACE,Campois TG,Araújo EJA,Svidizinski TIE,Felipe I

更新日期：2017-06-01 00:00:00

abstract：:Eight monoclonal antibodies (MAbs) were produced from mice immunised with whole cells of heat-killed Pasteurella multocida type A which had been cultured under iron-restricted conditions. The MAbs were selected by an enzyme-linked immunosorbent assay (ELISA) in which the antigen consisted of whole bacteria of the immu...

journal_title：Journal of medical microbiology

authors： Wijewardana TG,Wilson CF,Gilmour NJ,Poxton IR

更新日期：1990-12-01 00:00:00

abstract：:Helicobacter pylori is classified as a class I carcinogenic factor and its persistent colonization in the stomach induces gastric cancer. Cancerous Inhibitor of PP2A (CIP2A) is a newly identified oncoprotein overexpressed in gastric cancer. Serving as a key oncoprotein, CIP2A also participates in regulation of senesce...

journal_title：Journal of medical microbiology

authors： Zhao D,Liu Z,Ding J,Li W,Sun Y,Yu H,Zhou Y,Zeng J,Chen C,Jia J

更新日期：2010-03-01 00:00:00

abstract：:A partially-purified neuraminidase from the mucinase complex of Vibrio cholerae was used to prepare a specific anti-neuraminidase antiserum in rabbits. When the neutralising potency of this serum against V. cholerae neuraminidase was assessed in conventional tests, the enzymic activity, as measured by thiobarbituric a...

journal_title：Journal of medical microbiology

authors： Stewart-Tull DE,Ollar RA

更新日期：1988-02-01 00:00:00

abstract：:Fifty clinical strains of Chlamydia trachomatis were studied by denaturing gradient gel electrophoresis (DGGE) of bacterial DNA amplified by the polymerase chain reaction (PCR). The strains belonged to the three most commonly encountered serovars in developed countries--D, E and F. Six reference strains, including the...

journal_title：Journal of medical microbiology

authors： Sayada C,Denamur E,Grandchamp B,Orfila J,Elion J

更新日期：1995-07-01 00:00:00

abstract：:Introduction. The worldwide emergence of carbapenem resistance in Gram-negative bacteria makes the development of simple tests mandatory to identify antimicrobial resistance mechanisms. Enzymatic and membrane barriers are the prominent resistance mechanisms described in these bacteria. Several tests are currently used...

journal_title：Journal of medical microbiology

authors： Pinet E,Franceschi C,Collin V,Davin-Regli A,Zambardi G,Pagès JM

更新日期：2020-01-01 00:00:00

abstract：:The plasmid profiles, and their association with antimicrobial resistance, of 60 strains of Aeromonas hydrophila isolated from fish, shellfish and water were investigated. Only two strains were susceptible to all the antimicrobial agents tested; the highest incidences of resistance were to tetracycline (96.7%), prysta...

journal_title：Journal of medical microbiology

authors： Borrego JJ,Moriñigo MA,Martinez-Manzanares E,Bosca M,Castro D,Barja JL,Toranzo AE

更新日期：1991-11-01 00:00:00