Studies on the Vibrio cholerae mucinase complex. III. Neutralisation of the neuraminidase activity by specific anti-neuraminidase IgG.

Abstract:

:A partially-purified neuraminidase from the mucinase complex of Vibrio cholerae was used to prepare a specific anti-neuraminidase antiserum in rabbits. When the neutralising potency of this serum against V. cholerae neuraminidase was assessed in conventional tests, the enzymic activity, as measured by thiobarbituric acid, methoxyphenol-neuraminate and goblet-cell assays, apparently increased. These results are attributable to the presence of a sialylated glycoprotein substrate and small amounts of sialidase in the crude antiserum. However, a twice-purified DEAE-IgG fraction of the antiserum neutralised the enzymic activity of the V. cholerae neuraminidase.

journal_name

J Med Microbiol

authors

Stewart-Tull DE,Ollar RA

doi

10.1099/00222615-25-2-123

subject

Has Abstract

pub_date

1988-02-01 00:00:00

pages

123-8

issue

2

eissn

0022-2615

issn

1473-5644

journal_volume

25

pub_type

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