Abstract:
:A partially-purified neuraminidase from the mucinase complex of Vibrio cholerae was used to prepare a specific anti-neuraminidase antiserum in rabbits. When the neutralising potency of this serum against V. cholerae neuraminidase was assessed in conventional tests, the enzymic activity, as measured by thiobarbituric acid, methoxyphenol-neuraminate and goblet-cell assays, apparently increased. These results are attributable to the presence of a sialylated glycoprotein substrate and small amounts of sialidase in the crude antiserum. However, a twice-purified DEAE-IgG fraction of the antiserum neutralised the enzymic activity of the V. cholerae neuraminidase.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Stewart-Tull DE,Ollar RAdoi
10.1099/00222615-25-2-123subject
Has Abstractpub_date
1988-02-01 00:00:00pages
123-8issue
2eissn
0022-2615issn
1473-5644journal_volume
25pub_type
杂志文章abstract:PURPOSE:This paper aims to evaluate the antimicrobial resistance of Esherichia coli isolates from children under 5 years old, with and without diarrhoea, who were hospital outpatients in Culiacan, Sinaloa, Mexico. It also looks at the antimicrobial activity of fruit extracts against selected multidrug-resistant (MDR) E...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000548
更新日期:2017-07-01 00:00:00
abstract:PURPOSE:Penicillium is the most common mould isolated in housing. Penicillium chrysogenum is the only species tested by prick test or serology for allergic patients. The American Institute of Medicine has accepted Penicillium as an aetiological agent of rhinitis in children and adults and as an asthma agent in children...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000960
更新日期:2019-05-01 00:00:00
abstract::Leishmaniasis consists of a complex spectrum of infectious diseases with worldwide distribution of which visceral leishmaniasis or kala-azar caused by Leishmania donovani is the most devastating. In the absence of vaccines, chemotherapy remains the mainstay for the control of leishmaniasis. The drugs of choice are exp...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.064709-0
更新日期:2014-01-01 00:00:00
abstract::A collection of 820 isolates of streptococci and aerococci from human blood or internal organs was classified by means of a set of cultural and biochemical tests. Of these, 719 (712 streptococci and 7 aerococci) were from patients believed to be suffering from a systemic infection: endocarditis 317; a purulent lesion ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-9-3-275
更新日期:1976-08-01 00:00:00
abstract::This study investigated the comparative performance of the Amplicor assay and an in-house semi-automated, multiplex real-time PCR for the diagnosis of genital chlamydial infection. Four different assays, the COBAS Amplicor CT test (Amplicor PCR), in-house real-time PCR (IHRT-PCR), in-house nested cryptic plasmid PCR a...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46762-0
更新日期:2007-03-01 00:00:00
abstract::Sixty-one isolates of Candida albicans were tested for their phospholipase activity and this parameter was correlated with pathogenicity in albino mice. Of the isolates tested, 57 (93%) showed appreciable phospholipase activity and of these, 55 (90%) were pathogenic to mice. A significant correlation was found between...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-47-2-99
更新日期:1998-02-01 00:00:00
abstract:PURPOSE:We previously identified an association between CC22 meticillin-resistant Staphylococcus aureus (MRSA) bloodstream infection isolates with an elevated vancomycin MIC (V-MIC) in the susceptible range (1.5-2 mg l-1) and endocarditis. This study explores whether these isolates have a specific phenotype consistent ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000470
更新日期:2017-05-01 00:00:00
abstract::A monospecific polyclonal antiserum, prepared against Bacteroides fragilis common polysaccharide antigen purified by polyacrylamide gel immunoblot detected B. fragilis, B. thetaiotaomicron, B. ovatus and Prevotella melaninogenica in pus samples from various anatomical sites by immunofluorescence microscopy of the pus....
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-43-2-99
更新日期:1995-08-01 00:00:00
abstract::In-vitro proteinase production by oral Candida albicans isolates from patients with and without HIV infection (18 isolates from each group) was assessed by image analysis of a plate assay, with bovine serum albumin (BSA) as a substrate. The effect of sub-minimal inhibitory concentrations (sub-MICs) of nystatin, amphot...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-44-4-311
更新日期:1996-04-01 00:00:00
abstract::Data on the occurrence and epidemiology of Aspergillus spp. in laying hens farms are scant. With the aims of determining levels of airborne contamination in laying hen farms and evaluating the potential risk of infection for workers and animals, 57 air samples from 19 sheds (Group I), 69 from faeces (Group II), 19 fro...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.065946-0
更新日期:2014-03-01 00:00:00
abstract::Following characterisation by phenotypic tests and amplified ribosomal DNA restriction analysis (ARDRA), 50 tetracycline-resistant (MIC > or = 16 mg/L) Acinetobacter strains from clinical (n = 35) and aquatic (n = 15) samples were analysed by PCR for tetracycline resistance (Tet) determinants of classes A-E. All the c...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/0022-1317-49-10-929
更新日期:2000-10-01 00:00:00
abstract::The genetic characteristics of Vibrio parahaemolyticus strains isolated in 2004 and 2005 in Mozambique were assessed in this study to determine whether the pandemic clone of V. parahaemolyticus O3 : K6 and O4 : K68 serotypes has spread to Mozambique. Fifty-eight V. parahaemolyticus strains isolated from hospitalized d...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.2008/004275-0
更新日期:2008-12-01 00:00:00
abstract::The heparin-binding properties of six different species of coagulase-negative staphylococci were examined by a particle agglutination assay. Heparin (mol. wt 4000-6000), mildly treated with sodium periodate, was covalently coupled to amino-modified latex beads (0.72 micron diameter). The particle agglutination assay w...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-45-4-263
更新日期:1996-10-01 00:00:00
abstract::The production of toxins A and B by Clostridium difficile was greatly enhanced under biotin-limited conditions, in which a 140-kDa protein was expressed strongly. Gene cloning revealed that this protein was a homologue of formylglycinamidine ribonucleotide synthetase (FGAM synthetase, EC 6.3.5.3), which is known as Pu...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/0022-1317-51-1-34
更新日期:2002-01-01 00:00:00
abstract::Haemophilus influenzae serotype b (Hib) vaccines have reduced the amount of invasive Hib disease in immunised infants. However, Hib disease remains in unvaccinated infants and adults and non-capsulate H. influenzae (NCHi) still causes infections, including outbreaks of respiratory disease. Characterisation of strains ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-46-3-204
更新日期:1997-03-01 00:00:00
abstract::A multiplex PCR was developed to detect 15 serotypes of Streptococcus suis. This multiplex PCR was separated into two reaction sets. The first set identified nine serotypes (serotypes 1/2, 1, 2, 3, 4, 7, 9, 14 and 16) and the second set identified six serotypes (serotypes 5, 8, 10, 19, 23 and 25). This assay correctly...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.048587-0
更新日期:2012-12-01 00:00:00
abstract::The characterization of integrons and their promoters in 156 antibiotic-resistant clinical isolates of the important zoonotic pathogens Salmonella enterica subsp. enterica serovars Typhimurium (S. Typhimurium) and Enteritidis (S. Enteritidis) from Norwegian hospitals were performed. Integrons were found in 64 of 66 S....
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.04958-0
更新日期:2003-02-01 00:00:00
abstract::To gain insight into the value of lipopolysaccharide (LPS) core determinants for cross-protective immunisation the serological relationships between six complete (LPS) core types from Enterobacteriaceae were investigated. Hyperimmune sera were raised in mice by repeated immunisation with heat-killed strains of Salmone...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-48-5-433
更新日期:1999-05-01 00:00:00
abstract:PURPOSE:To determine the predominant strains of Bordetella pertussis in Greece during 2010-2015. METHODOLOGY:Infants and children (n=1150) (15 days to 14 years) of Greek, Roma and immigrant origin with different vaccination statuses were hospitalized in Athens, Greece with suspected pertussis infection. IS481/IS1001 r...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000688
更新日期:2018-03-01 00:00:00
abstract:PURPOSE:Despite hundreds of reports on the isolation of Nocardia from clinical samples, the presence and diversity of Nocardia species that are capable of survival in a harsh and adverse condition, such as a hospital environment, have not been comprehensively studied. The aim of this study was to assess Nocardia specie...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000436
更新日期:2017-03-01 00:00:00
abstract::Immunoblotting sera from cases of Streptococcus mutans or S. sobrinus endocarditis against an extract from S. sobrinus strain MUCOB 263 had identified three immunodominant antigenic bands at 190, 200 and 220 kDa. A lambda ZAPII DNA library was produced from the sheared genomic DNA of S. sobrinus MUCOB 263 and six iden...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-40-5-330
更新日期:1994-05-01 00:00:00
abstract::We present a case of septicaemia caused by Arcanobacterium haemolyticum in a previously healthy 23-year-old man suffering from acute pharyngotonsillitis, who developed complicated Lemierre's syndrome. Three blood cultures (both aerobic and anaerobic) revealed the exclusive presence of A. haemolyticum. The presence of ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.007492-0
更新日期:2009-12-01 00:00:00
abstract::Clostridium difficile is the most common cause of diarrhoea in hospitalised patients. Bacterial adherence to gut epithelial cells is a likely prerequisite to infection and toxin production. A novel flow cytometric method was developed for detecting adherence of C. difficile to human colonic and small intestinal epithe...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/0022-1317-50-6-526
更新日期:2001-06-01 00:00:00
abstract::Introduction. Current testing practices for yersiniosis mean that its true incidence and epidemiology are not well understood. In mid-2016, the introduction of testing via a multiplex gastrointestinal PCR panel at Portsmouth hospital laboratory in Hampshire, UK, resulted in a marked increase in the number of Yersinia ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.001125
更新日期:2020-03-01 00:00:00
abstract::Certain bile acids inhibit the growth in vitro of organisms commonly found in the intestine and, in particular, anaerobic bacteria. As the importance of this effect in vivo has not been demonstrated, we investigated changes in intestinal flora in the rat after diversion of bile to the urinary bladder. Five days after ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-10-2-241
更新日期:1977-05-01 00:00:00
abstract::Rapid identification of isolates in positive blood cultures are of great importance to secure correct treatment of septicaemic patients. As antimicrobial resistance is increasing, rapid detection of resistance is crucial. Carbapenem resistance in Bacteroides fragilis associated with cfiA-encoded class B metallo-beta-l...
journal_title:Journal of medical microbiology
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doi:10.1099/jmm.0.075465-0
更新日期:2014-08-01 00:00:00
abstract::Spirochaetes were demonstrated in material from patients with Lyme disease by short-time high-concentrate silver impregnation after treatment with amylase. Removal of mucoid material was essential to visualise Borrelia burgdorferi. Lyme spirochaetes were demonstrated in material from 23 patients with Lyme disease--ery...
journal_title:Journal of medical microbiology
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doi:10.1099/00222615-23-3-261
更新日期:1987-05-01 00:00:00
abstract::A study was undertaken to quantify the expression of prostaglandin (PG) receptors and find the effect of gestational age on expression of PG receptor genes in Chlamydia trachomatis-infected recurrent spontaneous aborters (RSA). Endometrial curettage tissue (ECT) was collected from 130 RSA (Group I) and 100 age-matched...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000256
更新日期:2016-06-01 00:00:00
abstract::The antifungal effects of cinnamaldehyde, 4-hydroxy-3-methoxycinnamaldehyde (coniferyl aldehyde) and 3,5-dimethoxy-4-hydroxycinnamaldehyde (sinapaldehyde) were investigated against 65 strains of Candida (six standard, 39 fluconazole-sensitive and 20 fluconazole-resistant). MICs of cinnamaldehyde, coniferyl aldehyde an...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.036145-0
更新日期:2013-02-01 00:00:00
abstract::A case of Salmonella typhimurium pericarditis is reported. The diagnosis was based on blood and pericardial effusion cultures. ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.05449-0
更新日期:2004-10-01 00:00:00