Opsonic requirements of Helicobacter pylori.

Abstract:

:The opsonic requirements of Helicobacter pylori were investigated in a series of experiments with human polymorphonuclear leucocytes (PMNL). Pre-incubation of H. pylori with pooled normal human serum (NHS) in concentrations of 5-20% significantly increased the uptake of radiolabelled bacteria by PMNL. Treatment of the bacteria with NHS 30% caused the release of radiolabel and this effect was abolished by heating serum to 56 degrees C, suggesting that H. pylori is serum-sensitive and that complement is involved. Opsonisation of H. pylori with NHS concentrations of 10-30% significantly increased PMNL chemiluminescence. Removal of specific antibody had no effect. Removal of either the classical or alternative complement pathways produced no significant change in PMNL chemiluminescence, indicating that either pathway is sufficient for opsonisation on its own. The results confirm that complement is the most efficient opsonin for H. pylori.

journal_name

J Med Microbiol

authors

McKinlay AW,Young A,Russell RI,Gemmell CG

doi

10.1099/00222615-38-3-209

subject

Has Abstract

pub_date

1993-03-01 00:00:00

pages

209-15

issue

3

eissn

0022-2615

issn

1473-5644

journal_volume

38

pub_type

杂志文章
  • Molecular detection of Mycobacterium tuberculosis in poor-quality cough specimens.

    abstract::Clinical specimens unfit for laboratory processing represent missed opportunities for diagnosing tuberculosis. Poor-quality cough specimens (n=61) from presumptive tuberculosis cases were cultured and GeneXpert MTB/RIF (Xpert) successfully performed on samples transferred by flocked swab into PrimeStore molecular tran...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001239

    authors: Mboneni TA,Eales OO,Mosina NL,Fourie PB

    更新日期:2020-09-01 00:00:00

  • Evaluation of risk factors for colistin resistance among uropathogenic isolates of Escherichia coli and Klebsiella pneumoniae: a case-control study.

    abstract:INTRODUCTION:The last few years have seen the emergence of multi-drug resistant (MDR) Gram-negative infections, which are associated with high morbidity and mortality. The indiscriminate use of colistin has led to the development of resistance, which can be diagnosed effectively by broth microdilution. Studies from Ind...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000986

    authors: Kaza P,Mahindroo J,Veeraraghavan B,Mavuduru RS,Mohan B,Taneja N

    更新日期:2019-06-01 00:00:00

  • Cell damage caused by vaginal Candida albicans isolates from women with different symptomatologies.

    abstract::The present study aimed to characterize cell damage caused by vaginal Candida albicans isolates from women with different symptomatologies. It was evaluated 12 clinical isolates of C. albicans from vaginal samples: 4 from asymptomatic women (AS), 4 from women with a single episode of vulvovaginal candidiasis (VVC) and...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000547

    authors: Faria DR,Sakita KM,Akimoto-Gunther LS,Kioshima ÉS,Svidzinski TIE,Bonfim-Mendonça PS

    更新日期:2017-08-01 00:00:00

  • Impedance monitoring of bacterial activity.

    abstract::Bacterial activity and growth were monitored by following the changes of electrical impedance of cultures in liquid media. The signal is expressed automatically as a curve similar to grwoth curves produced by other methods. The technique offers a new, rapid and sensitive means of detecting active micro-organisms and i...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-8-1-19

    authors: Ur A,Brown DF

    更新日期:1975-02-01 00:00:00

  • Comparative characterisation of the biofilm-production abilities of Staphylococcus epidermidis isolated from human skin and platelet concentrates.

    abstract:PURPOSE:Staphylococcus epidermidis is the predominant contaminant of platelet concentrates (PCs), a blood product used to treat patients with platelet deficiencies. This microorganism is able to form surface-attached aggregates (biofilms) in human skin. Herein, the abundance of S. epidermidis biofilm-producers in conta...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000673

    authors: Taha M,Kohnen C,Mallya S,Kou Y,Zapata A,Ramirez-Arcos S

    更新日期:2018-02-01 00:00:00

  • Association of Clostridium difficile ribotype 078 with detectable toxin in human stool specimens.

    abstract::Using a Clostridium difficile glutamate dehydrogenase (GDH) immunoassay and a sensitive C. difficile toxin A/B immunoassay, human stool specimens from patients with diarrhoea (n = 1085) were classified as either GDH positive/toxin negative, or GDH positive/toxin positive. Overall, 528/725 (73%) of the GDH-positive/tox...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000165

    authors: Fairley DJ,McKenna JP,Stevenson M,Weaver J,Gilliland C,Watt A,Coyle PV

    更新日期:2015-11-01 00:00:00

  • Widespread clone of Burkholderia cenocepacia in cystic fibrosis patients in the Czech Republic.

    abstract::The morbidity and mortality rates in patients with cystic fibrosis (CF) are significantly affected by infections with Burkholderia cepacia complex. In a Czech CF Centre, the prevalence of the infection reached up to 30 %, with the majority of patients found to be infected with Burkholderia cenocepacia (formerly genomo...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46025-0

    authors: Drevinek P,Vosahlikova S,Cinek O,Vavrova V,Bartosova J,Pohunek P,Mahenthiralingam E

    更新日期:2005-07-01 00:00:00

  • Inhibition of quorum-sensing-controlled virulence factors of Pseudomonas aeruginosa by Murraya koenigii essential oil: a study in a Caenorhabditis elegans infectious model.

    abstract::The global emergence of antibiotic-resistant strains of Pseudomonas aeruginosa poses a major threat in both hospital environments and the community. P. aeruginosa is an opportunistic human pathogen, and it also infects a wide range of model organisms including the nematode Caenorhabditis elegans. Quorum sensing (QS) m...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000385

    authors: Ganesh PS,Rai RV

    更新日期:2016-12-01 00:00:00

  • Experimental infection of germ-free mice with hyper-toxigenic enterohaemorrhagic Escherichia coli O157:H7, strain 6.

    abstract::A mouse enterohaemorrhagic Escherichia coli (EHEC) infection model was developed with a combination of germ-free (GF) mice and hyper-toxigenic EHEC (HT-EHEC) O157:H7 strain 6. The HT-EHEC strain 6 produced both Shiga-like toxin (SLT)-1 1.0 microg/mI and SLT-2 8.2 microg/ml in vitro. Eight-week-old germ-free mice were ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-51-4-336

    authors: Taguchi H,Takahashi M,Yamaguchi H,Osaki T,Komatsu A,Fujioka Y,Kamiya S

    更新日期:2002-04-01 00:00:00

  • Cronobacter sakazakii induces necrotizing enterocolitis by regulating NLRP3 inflammasome expression via TLR4.

    abstract::Introduction. Neonatal infection with Cronobacter sakazakii can cause severe intestinal damage and necrotizing enterocolitis (NEC). The inflammasome and Toll-like receptors mediate intestinal damage caused by other intestinal pathogens causing NEC, but the exact mechanism is unclear.Aim. We evaluated the molecular mec...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001181

    authors: Chen Z,Zhang Y,Lin R,Meng X,Zhao W,Shen W,Fan H

    更新日期:2020-05-01 00:00:00

  • Mediterranean spotted fever with encephalitis.

    abstract::Rickettsia conorii infection is endemic in the Mediterranean basin, where it is known as Mediterranean spotted fever, also known as Boutonneuse fever and Marseilles fever. We report the case of a 66-year-old diabetic man who presented a severe form of the disease, complicated by acute renal failure, thrombocytopenia a...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.1099/jmm.0.004465-0

    authors: Aliaga L,Sánchez-Blázquez P,Rodríguez-Granger J,Sampedro A,Orozco M,Pastor J

    更新日期:2009-04-01 00:00:00

  • Do English NHS Microbiology laboratories offer adequate services for the diagnosis of UTI in children? Healthcare Quality Improvement Partnership (HQIP) Audit of Standard Operational Procedures.

    abstract::The National Institute of Care Excellence (NICE) 2007 guidance CG54, on urinary tract infection (UTI) in children, states that clinicians should use urgent microscopy and culture as the preferred method for diagnosing UTI in the hospital setting for severe illness in children under 3 years old and from the GP setting ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000114

    authors: McNulty CAM,Verlander NQ,Moore PCL,Larcombe J,Dudley J,Banerjee J,Jadresic L

    更新日期:2015-09-01 00:00:00

  • In vitro activities of vancomycin and linezolid against biofilm-producing methicillin-resistant staphylococci species isolated from catheter-related bloodstream infections from an Egyptian tertiary hospital.

    abstract:PURPOSE:Catheter-related bloodstream infections (CRBSIs) are among the most common hospital-acquired infections. We aimed to survey methicillin resistance, biofilm production and susceptibility to vancomycin, linezolid and other antibiotics for staphylococci isolated from CRBSIs. METHODOLOGY:Fifty-eight isolates [20 S...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000490

    authors: Hashem AA,Abd El Fadeal NM,Shehata AS

    更新日期:2017-06-01 00:00:00

  • The potential of fosfomycin for multi-drug resistant sepsis: an analysis of in vitro activity against invasive paediatric Gram-negative bacteria.

    abstract:PURPOSE:Antimicrobial resistance (AMR) is of increasing global concern, threatening to undermine recent progress in reducing child and neonatal mortality. Repurposing older antimicrobials is a prominent strategy to combat multidrug-resistant sepsis. A potential agent is fosfomycin, however, there is scarce data regardi...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000973

    authors: Williams PCM,Waichungo J,Gordon NC,Sharland M,Murunga S,Kamau A,Berkley JA

    更新日期:2019-05-01 00:00:00

  • The role of HSV-induced Fc- and C3b (i)-receptors in bacterial adherence.

    abstract::Herpes simplex virus type-1 (HSV-1) induces Fc- and C3b(i)-receptors on infected cells. The role of these receptors in bacterial superinfection was studied by comparing the adherence of non-opsonised and opsonised bacteria to HSV-infected and non-infected HEp-2 cells. A flow cytometric adherence assay, based on the fl...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-40-1-48

    authors: De Graaf-Miltenburg LA,Van Vliet KE,Ten Hagen TL,Verhoef J,Van Strijp JA

    更新日期:1994-01-01 00:00:00

  • Streptococcus gordonii pheromone s.g.cAM373 may influence the reservoir of antibiotic resistance determinants of Enterococcus faecalis origin in the oral metagenome.

    abstract::Streptococcus gordonii produces a pheromone heptapeptide, s.g.cAM373, which induces a conjugative mating response in Enterococcus faecalis cells carrying the responsive plasmid, pAM373. We investigated the extent of this intergeneric signaling on DNA acquisition by streptococcal species likely to cohabit oral biofilms...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000613

    authors: Mansfield JM,Herrmann P,Jesionowski AM,Vickerman MM

    更新日期:2017-11-01 00:00:00

  • An in vitro and in vivo study on the synergistic effect and mechanism of itraconazole or voriconazole alone and in combination with tetrandrine against Aspergillus fumigatus.

    abstract::In this study, we investigated the in vitro antifungal effects of itraconazole/voriconazole (ITR/VRC) alone and in combination with tetrandrine (TET) against 23 clinical isolates of A. fumigatus using a chequerboard microdilution method. The dynamic antifungal effects of TET with ITR/VRC against A. fumigatus were asse...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000120

    authors: Li SX,Song YJ,Zhang LL,Shi JP,Ma ZL,Guo H,Dong HY,Li YM,Zhang H

    更新日期:2015-09-01 00:00:00

  • Ribotype differences between clinical and environmental isolates of Burkholderia pseudomallei.

    abstract::Burkholderia pseudomallei is isolated frequently from the soil in regions where the disease melioidosis occurs. However, recent surveys in Thailand have shown that the frequency of isolation of the organism from soil samples is not directly related to the incidence of melioidosis in an area. To determine whether strai...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-46-7-565

    authors: Trakulsomboon S,Dance DA,Smith MD,White NJ,Pitt TL

    更新日期:1997-07-01 00:00:00

  • Prospective evaluation of the new chromogenic medium CandiSelect 4 for differentiation and presumptive identification of the major pathogenic Candida species.

    abstract::The rapid identification of pathogenic yeasts is a crucial step in ensuring that effective antifungal treatment is started as early as possible. CandiSelect 4 (CS4; Bio-Rad) is a new chromogenic medium for the isolation of fungi, the direct identification of Candida albicans and the presumptive identification of the m...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46715-0

    authors: Sendid B,François N,Standaert A,Dehecq E,Zerimech F,Camus D,Poulain D

    更新日期:2007-04-01 00:00:00

  • Experimental infection of Wistar rats with 'Gastrospirillum suis'.

    abstract::In order to develop a model for the study of gastric spiral bacteria, and based on the observation that Wistar rats do not carry urease-positive spiral bacteria in their gastric mucosa, mucus from a pig naturally colonised by 'Gastrospirillum suis' (an organism with I6S rDNA 99.5% similar to that of 'G. hominis' type ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-44-2-105

    authors: Mendes EN,Queiroz DM,Coimbra RS,Moura SB,Barbosa AJ,Rocha GA

    更新日期:1996-02-01 00:00:00

  • Prevalence of Chlamydia psittaci in the feral pigeon population of Basel, Switzerland.

    abstract::Feral pigeons (Columba livia) are commonly infected with Chlamydia psittaci, the agent of psittacosis in humans. To assess the risk of zoonosis posed by feral pigeons in the urban environment, we determined the prevalence of Chlamydia psittaci by detection of the outer-membrane protein A (ompA) gene of this pathogen i...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.034025-0

    authors: Geigenfeind I,Vanrompay D,Haag-Wackernagel D

    更新日期:2012-02-01 00:00:00

  • Identification of oral bacteria associated with crevicular epithelial cells from chronic periodontitis lesions.

    abstract::Bacterial invasion of host epithelial cells plays an important role in the pathogenesis of periodontal diseases; however, the interactions between subgingival species and the gingival crevice cells are not fully understood. This study determined the prevalence of a group of oral bacterial species on or in epithelial c...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46417-0

    authors: Colombo AV,Silva CM,Haffajee A,Colombo APV

    更新日期:2006-05-01 00:00:00

  • Rapid and accurate identification of species belonging to the Candida parapsilosis complex by real-time PCR and melting curve analysis.

    abstract::Candida parapsilosis is the second most frequent Candida species isolated from blood cultures. Since 2005, C. parapsilosis has been divided into three distinct species based on genetic traits: Candida parapsilosis, Candida metapsilosis and Candida orthopsilosis. The aim of this study was to develop a rapid real-time P...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.026633-0

    authors: Hays C,Duhamel C,Cattoir V,Bonhomme J

    更新日期:2011-04-01 00:00:00

  • The isolation of Streptococcus pyogenes from throat swabs.

    abstract::Simulated throat swabs were prepared with known numbers of Streptococcus pyogenes; some were suspended in pasteurised human saliva and an equal number in saline. Two types of commercially available swabs were tested; these were composed of (1) plain, buffered, cotton wool, and (2) albumen-coated cotton wool. The mean ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-10-1-69

    authors: Ross PW

    更新日期:1977-02-01 00:00:00

  • Biochemical characterisation of human isolates of Blastocystis hominis.

    abstract::SDS-PAGE and iso-enzyme analysis of 11 human isolates of Blastocystis hominis revealed at least two variants with different polypeptide patterns and two zymodemes, respectively. This is the first iso-enzyme and the second protein analysis to indicate strain differences in B. hominis. ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-42-4-304

    authors: Mansour NS,Mikhail EM,el Masry NA,Sabry AG,Mohareb EW

    更新日期:1995-04-01 00:00:00

  • In vitro assessment of the APTIMA Combo 2 assay for the detection of Chlamydia trachomatis using highly purified elementary bodies.

    abstract::The Gen-Probe APTIMA Combo 2 assay has previously been reported to have a high sensitivity. The end point of this assay was evaluated using highly purified chlamydial elementary bodies (EBs). The performance of the APTIMA Combo 2 assay was compared with a commercially available PCR kit, AMPLICOR Chlamydia trachomatis....

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45911-0

    authors: Ikeda-Dantsuji Y,Konomi I,Nagayama A

    更新日期:2005-04-01 00:00:00

  • The prevalence of Chlamydia trachomatis in fresh tissue specimens from patients with ectopic pregnancy or tubal factor infertility as determined by PCR and in-situ hybridisation.

    abstract::The prevalence of chlamydial DNA determined by PCR and in-situ hybridisation (ISH) in fresh tissue specimens (endometrium, fallopian tube and ovary) was investigated in 33 women presenting with ectopic pregnancy (EP), 14 women with tubal factor infertility (TFI) and 50 control patients from the UK and the West Indies....

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-50-10-902

    authors: Barlow REL,Cooke ID,Odukoya O,Heatley MK,Jenkins J,Narayansingh G,Ramsewak SS,Eley A

    更新日期:2001-10-01 00:00:00

  • Prevalence of resistant Helicobacter pylori isolates in Bulgarian children.

    abstract::The aim of this study was to assess the primary and combined resistances of Helicobacter pylori isolates obtained from paediatric patients in 2000-2001 to seven antimicrobial agents. Resistance rates of pre-treatment isolates from 115 children were investigated by the limited agar dilution method alone and by the E-te...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-51-9-786

    authors: Boyanova L,Koumanova R,Gergova G,Popova M,Mitov I,Kovacheva Y,Derejian S,Katsarov N,Nikolov R,Krastev Z

    更新日期:2002-09-01 00:00:00

  • Maternofetal infections due to Eikenella corrodens.

    abstract::Eikenella corrodens, a commensal of the human oral cavity, is generally associated with bite wounds and head and neck infections. Neonatal infections are rare. We report two cases of premature birth associated with maternofetal E. corrodens infection. ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.002568-0

    authors: Garnier F,Masson G,Bedu A,Masson P,Decroisette E,Guigonis V,Fermeaux V,De Barbentane MC,Denis F,Ploy MC

    更新日期:2009-02-01 00:00:00

  • Effects of altering the germination potential of Bacillus anthracis spores by exogenous means in a mouse model.

    abstract::Inhalational anthrax is the most severe form of anthrax. It has been shown in small-animal and non-human primate models that relatively large pools of ungerminated Bacillus anthracis spores can remain within the alveolar spaces for days to weeks post-inhalation or until transported to areas more favourable for germina...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.008656-0

    authors: Cote CK,Bozue J,Twenhafel N,Welkos SL

    更新日期:2009-06-01 00:00:00