The effects of ethyleneglycoltetra-acetic acid on bactericidal activity of human serum against Escherichia coli.

Abstract:

:The effects of ethyleneglycoltetra-acetic acid (EGTA) and EGTA + magnesium (MgEGTA) on the viable counts of 10 strains of Escherichia coli O6 have been studied in normal human serum (NHS), heat-inactivated serum (HIS) and in culture media with and without the addition of a beta-lactam antibiotic. The addition of EGTA to NHS largely prevented bactericidal activity against serum-sensitive strains while, in contrast, it reduced the growth of a serum-resistant strain. These apparently paradoxical effects are due to the lower growth rate permitted by the reduced amount of available magnesium in the presence of EGTA. Experiments with equimolar concentrations of EGTA and magnesium indicated that whilst MgEGTA is a reagent allowing alternative complement-pathway activity, such activity must be determined by comparison with results in HIS + MgEGTA rather than in HIS alone, classical-pathway activity being taken as the difference between the results in NHS and in NHS + MgEGTA. By these criteria, prompt killing by serum was found to occur via the classical pathway while delayed serum bactericidal activity occurred by the alternative pathway in some strains and by the classical pathway in others.

journal_name

J Med Microbiol

authors

Roberts AP,Phillips R

doi

10.1099/00222615-14-2-195

subject

Has Abstract

pub_date

1981-05-01 00:00:00

pages

195-203

issue

2

eissn

0022-2615

issn

1473-5644

journal_volume

14

pub_type

杂志文章
  • A simple phenotypic test for detecting the contribution of outer membrane permeability to carbapenem resistance.

    abstract::Introduction. The worldwide emergence of carbapenem resistance in Gram-negative bacteria makes the development of simple tests mandatory to identify antimicrobial resistance mechanisms. Enzymatic and membrane barriers are the prominent resistance mechanisms described in these bacteria. Several tests are currently used...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001129

    authors: Pinet E,Franceschi C,Collin V,Davin-Regli A,Zambardi G,Pagès JM

    更新日期:2020-01-01 00:00:00

  • Phenotypic and genotypic traits and epidemiological implication of Vibrio cholerae O1 and O139 strains in India during 2003.

    abstract::During 2003, Vibrio cholerae O1 Ogawa was the predominant serotype among diarrhoeal patients admitted to different hospitals in India. With the exception of 3 strains from Kolkata, none of 172 strains examined exhibited resistance to tetracycline, but 45.7 % showed reduced susceptibility to ciprofloxacin. Extensive mo...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46982-0

    authors: Chatterjee S,Ghosh K,Raychoudhuri A,Pan A,Bhattacharya MK,Mukhopadhyay AK,Ramamurthy T,Bhattacharya SK,Nandy RK

    更新日期:2007-06-01 00:00:00

  • The establishment and persistence of Ureaplasma urealyticum in oestradiol-treated female mice.

    abstract::Administration of oestradiol to three strains of female mice induced the oestrous phase of the reproductive cycle in which there are few or no polymorphonuclear leucocytes (PMNL) in vaginal smears. This treatment rendered the mice susceptible to genital tract colonisation by serotype 8 of Ureaplasma urealyticum, inocu...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-29-2-111

    authors: Furr PM,Taylor-Robinson D

    更新日期:1989-06-01 00:00:00

  • Effect of glucan on Leishmania major infection in BALB/c mice.

    abstract::The effect of glucan on Leishmania major infection was studied in BALB/c mice, which are highly susceptible to leishmania infection. Glucan (0.45 mg), or isovolumetric dextrose, was administered intraperitoneally 7, 5, 3 and 1 day before infection with L. major promastigotes. At 3, 5, 6, 8 and 10 weeks after infection...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-23-4-363

    authors: Al Tuwaijri AS,Mahmoud AA,Al Mofleh IA,Al Khuwaitir SA

    更新日期:1987-06-01 00:00:00

  • Continuous culture of sessile human oropharyngeal microbiotas.

    abstract::The microbiota of the human oropharynx plays an important role in health through involvement in the aetiology of infection and the carriage of adventitious pathogens. Despite this, there are few models available for the preclinical assessment of novel antimicrobials directed to the human throat. We have profiled bacte...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.055806-0

    authors: Humphreys GJ,McBain AJ

    更新日期:2013-06-01 00:00:00

  • Listeria monocytogenes cells under nutrient deprivation showed reduced ability to infect the human intestinal cell line HT-29.

    abstract:PURPOSE:This study aimed to evaluate the effect of two types of stress, cold and nutritional, on the viability and the in vitro virulence of the foodborne pathogenic bacteria Listeria monocytogenes. METHODOLOGY:Ten diverse isolates were kept in phosphate-buffered saline (PBS) at optimal (37 °C) or at refrigeration tem...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000648

    authors: Araujo V,Neves E,Silva AC,Martins APL,Brito LC

    更新日期:2018-01-01 00:00:00

  • Paradoxically high resistance of natural killer T (NKT) cell-deficient mice to Legionella pneumophila: another aspect of NKT cells for modulation of host responses.

    abstract::In the present study, we examined the roles of natural killer T (NKT) cells in host defence against Legionella pneumophila in a mouse model. The survival rate of NKT cell-deficient Jalpha281 knock-out (KO) mice was significantly higher than that of wild-type mice. There was no bacterial overgrowth in the lungs, but Ja...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.47747-0

    authors: Hayakawa K,Tateda K,Fuse ET,Matsumoto T,Akasaka Y,Ishii T,Nakayama T,Taniguchi M,Kaku M,Standiford TJ,Yamaguchi K

    更新日期:2008-11-01 00:00:00

  • Frequency of four different mutacin genes in Streptococcus mutans genotypes isolated from caries-free and caries-active individuals.

    abstract::The ability of Streptococcus mutans to produce mutacins, combined with the production of other virulence factors such as lactic acid, may contribute to the pathogenesis of this bacterium. In the present study, the detection of genes encoding mutacin types I/III, II and IV was performed by PCR with specific primers to ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45870-0

    authors: Kamiya RU,Napimoga MH,Höfling JF,Gonçalves RB

    更新日期:2005-06-01 00:00:00

  • Disseminated intravascular coagulation in rabbits: synergistic activity of meningococcal endotoxin and materials egested from leucocytes containing meningococci.

    abstract::Leucocyte-egested material was harvested after the quantitative in-vitro phagocytosis of Neisseria meningitidis by rabbit or mouse polymorphonuclear leucocytes. The egested material was injected subcutaneously into rabbits and followed 24 h later with an intravenous injection of what would by itself have been a non-le...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-9-4-451

    authors: DeVoe IW,Gilka F

    更新日期:1976-11-01 00:00:00

  • Identification and evaluation of LPS antigen for serodiagnosis of uveitis associated with leptospirosis.

    abstract::Leptospirosis is a widespread zoonotic disease that affects all mammals in different parts of the world. Though there are many commercial kits available for the diagnosis of systemic leptospirosis, the nature of the antigen has not been described. Therefore, identification of a specific antigen is important. Since ocu...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.05120-0

    authors: Priya CG,Bhavani K,Rathinam SR,Muthukkaruppan VR

    更新日期:2003-08-01 00:00:00

  • Adhesion to and invasion of pulmonary epithelial cells by the F15/LAM4/KZN and Beijing strains of Mycobacterium tuberculosis.

    abstract::Globally, specific genotypes of Mycobacterium tuberculosis have been shown to dominate in patients, suggesting that these are more successful pathogens. One such genotype, the F15/LAM4/KZN (KZN) family of M. tuberculosis, has predominated in KwaZulu-Natal, South Africa, since the early 1990s. This strain recently evol...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.016006-0

    authors: Ashiru OT,Pillay M,Sturm AW

    更新日期:2010-05-01 00:00:00

  • Isolation and characterisation of an anticryptococcal protein in human cerebrospinal fluid.

    abstract::An earlier study reported that human cerebrospinal fluid (CSF) has fungistatic activity for Cryptococcus neoformans. The present study reports that molecular sieve fractionation of concentrated CSF yielded three protein peaks, one of which (p2) had anticryptococcal activity. On a DEAE-Sephacel anion-exchange column th...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-50-1-83

    authors: Ahluwalia M,Brummer E,Sridhar S,Singh R,Stevens DA

    更新日期:2001-01-01 00:00:00

  • Biochemical characterisation, enteropathogenicity and antimicrobial resistance plasmids of clinical and environmental Aeromonas isolates.

    abstract::One hundred and eight strains of Aeromonas from clinical and environmental samples were speciated. Seven species were identified, the most prevalent of which was A. hydrophila. Experimental studies in an animal model with 36 representative strains of different species revealed that all strains could cause significant ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-44-6-434

    authors: Chaudhury A,Nath G,Shukla BN,Sanyal SC

    更新日期:1996-06-01 00:00:00

  • Identification and characterization of breakthrough contaminants associated with the conventional isolation of Mycobacterium tuberculosis.

    abstract::Breakthrough contamination of tuberculosis (TB) cultures is a problem in that it allows the overgrowth of another bacterium present in the sputum specimen, which can potentially mask the presence of Mycobacterium tuberculosis. The aim of this study was to isolate and characterize the bacterial organisms responsible fo...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.030619-0

    authors: McClean M,Stanley T,Stanley S,Maeda Y,Goldsmith CE,Shepherd R,Millar BC,Dooley JS,Moore JE

    更新日期:2011-09-01 00:00:00

  • Prospective evaluation of the new chromogenic medium CandiSelect 4 for differentiation and presumptive identification of the major pathogenic Candida species.

    abstract::The rapid identification of pathogenic yeasts is a crucial step in ensuring that effective antifungal treatment is started as early as possible. CandiSelect 4 (CS4; Bio-Rad) is a new chromogenic medium for the isolation of fungi, the direct identification of Candida albicans and the presumptive identification of the m...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46715-0

    authors: Sendid B,François N,Standaert A,Dehecq E,Zerimech F,Camus D,Poulain D

    更新日期:2007-04-01 00:00:00

  • K1, K5 and O antigens of Escherichia coli in relation to serum killing via the classical and alternative complement pathways.

    abstract::The sensitivity of Escherichia coli strains to 80% normal human serum (NHS) and the relative importance of the classical and alternative complement pathways was assessed in relation to K1, K5, and O antigen carriage. Strains of each of the common O-serogroups, O1, O2, O4, O6, O7, O9, O18 and O75, smooth strains not ty...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-41-2-139

    authors: Devine DA,Roberts AP

    更新日期:1994-08-01 00:00:00

  • Cytotoxin detection in Campylobacter jejuni strains of human and animal origin with three tissue culture assay systems.

    abstract::Cytotoxin (CTX) production in 34 human and 22 animal strains of Campylobacter jejuni isolated in Japan and other countries was studied by three assay systems described previously. Furthermore, cholera-like enterotoxin production by these strains was tested by reversed passive latex agglutination (RPLA). CTX titres in ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-43-5-354

    authors: Misawa N,Ohnishi T,Itoh K,Takahashi E

    更新日期:1995-11-01 00:00:00

  • Evaluation of internal transcribed spacer 2-RFLP analysis for the identification of dermatophytes.

    abstract::A total of 95 isolates, belonging to 33 species of five dermatophyte genera, i.e. Arthroderma (15 species), Chrysosporium (two), Epidermophyton (one), Microsporum (three) and Trichophyton (12), were studied using internal transcribed spacer 2 (ITS2)-PCR-RFLP analysis (ITS2-RFLP), consisting of amplification of the ITS...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.013870-0

    authors: De Baere T,Summerbell R,Theelen B,Boekhout T,Vaneechoutte M

    更新日期:2010-01-01 00:00:00

  • Detection of enterovirus 68 as one of the commonest types of enterovirus found in patients with acute respiratory tract infection in China.

    abstract::Human enterovirus 68 (HEV-68) is an enterovirus associated with respiratory illness. In China, no information about HEV-68 is available for children yet. This study aimed to investigate the presence of HEV-68 in mainland China between 2009 and 2012 and to explore the migration events of HEV-68 across the world. Among ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.068247-0

    authors: Lu QB,Wo Y,Wang HY,Wei MT,Zhang L,Yang H,Liu EM,Li TY,Zhao ZT,Liu W,Cao WC

    更新日期:2014-03-01 00:00:00

  • Distribution of sero-biotypes of Campylobacter jejuni and C. coli isolated from paediatric patients.

    abstract::During a one-year period, 258 isolates of Campylobacter jejuni and C. coli were obtained from children with gastroenteritis or bacteraemia at the Red Cross Children's Hospital, Cape Town, South Africa. These isolates were biotyped by hippurate hydrolysis, H2S production and tolerance to 2,3,5-triphenyltetrazolium chlo...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-21-1-1

    authors: Lastovica AJ,Le Roux E,Congi RV,Penner JL

    更新日期:1986-02-01 00:00:00

  • Pyrolysis mass spectrometry in epidemiological and population genetic studies of Haemophilus influenzae.

    abstract::Haemophilus influenzae serotype b (Hib) vaccines have reduced the amount of invasive Hib disease in immunised infants. However, Hib disease remains in unvaccinated infants and adults and non-capsulate H. influenzae (NCHi) still causes infections, including outbreaks of respiratory disease. Characterisation of strains ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-46-3-204

    authors: Leaves NI,Sisson PR,Freeman R,Jordens JZ

    更新日期:1997-03-01 00:00:00

  • Sepsis with prolonged hypotension due to Moraxella osloensis in a non-immunocompromised child.

    abstract::We report a case of septicaemia with prolonged, refractory hypotension related to Moraxella osloensis isolated in a non-immunocompromised paediatric patient. ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.016378-0

    authors: Dien Bard J,Lewinski M,Summanen PH,Deville JG

    更新日期:2011-01-01 00:00:00

  • Adaptive resistance to aminoglycoside antibiotics in Pseudomonas aeruginosa.

    abstract::Aminoglycoside-resistant variants of Pseudomonas aeruginosa strain PAO1 were readily selected by culturing the organism in medium containing increasing concentrations of gentamicin, tobramycin or amikacin until the strains were growing in a concentration of drug 128-fold greater than the minimal inhibitory concentrati...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-29-1-41

    authors: Gilleland LB,Gilleland HE,Gibson JA,Champlin FR

    更新日期:1989-05-01 00:00:00

  • Effect of inhibitors in clinical specimens on Taq and Tth DNA polymerase-based PCR amplification of influenza A virus.

    abstract::Fifteen randomly selected nasopharyngeal (NP) swab specimens (culture-negative for influenza A virus) were spiked with influenza A virus and the nucleic acids were extracted and subjected to PCR amplification with Thermus aquaticus (Taq) and T. thermophilus (Tth) DNA polymerases. Products of the expected size, and giv...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-47-12-1131

    authors: Poddar SK,Sawyer MH,Connor JD

    更新日期:1998-12-01 00:00:00

  • Isolation and characterisation of bacteria from abscesses in the subcutis of cats.

    abstract::Thirty-six closed abcesses in the subcutis of cats were examined. Of 168 bacterial strains isolated, 121(72%) were anaerobes and 47 (28%) were facultative anaerobes. Twenty-six abscesses contained mixtures of facultative anaerobes and anaerobes, six contained anaerobes only and four contained facultative anaerobes onl...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-12-2-207

    authors: Love DN,Jones RF,Bailey M,Johnson RS

    更新日期:1979-05-01 00:00:00

  • Plasmid-mediated resistance to gentamicin in Staphylococcus aureus: the involvement of a transposon.

    abstract::Resistance to gentamicin, tobramycin and kanamycin (GmrTmrKmr) in strains of Staphylococcus aureus isolated from clinical sources in Australia is mediated by a 4.7 kb transposable element, designated Tn4001. A 2.5 kb HindIII fragment which maps symmetrically within Tn4001, and encompasses the aminoglycoside-resistance...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-23-2-101

    authors: Lyon BR,Gillespie MT,Byrne ME,May JW,Skurray RA

    更新日期:1987-03-01 00:00:00

  • Molecular detection of Mycobacterium tuberculosis in poor-quality cough specimens.

    abstract::Clinical specimens unfit for laboratory processing represent missed opportunities for diagnosing tuberculosis. Poor-quality cough specimens (n=61) from presumptive tuberculosis cases were cultured and GeneXpert MTB/RIF (Xpert) successfully performed on samples transferred by flocked swab into PrimeStore molecular tran...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001239

    authors: Mboneni TA,Eales OO,Mosina NL,Fourie PB

    更新日期:2020-09-01 00:00:00

  • Sequential multiplex PCR for identifying pneumococcal capsular serotypes from South-Saharan African clinical isolates.

    abstract::A serial multiplex PCR approach was reformulated for pneumococcal serotyping to test 153 clinical isolates from children in Mozambique. This approach identified serotypes in 139 (90.8%) of 153 isolates; 126 (82.4%) were identified within two reactions. This approach in developing countries would require minimal traini...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.47346-0

    authors: Morais L,Carvalho MDG,Roca A,Flannery B,Mandomando I,Soriano-Gabarró M,Sigauque B,Alonso P,Beall B

    更新日期:2007-09-01 00:00:00

  • A simplified system for the identification of staphylococci by multipoint inoculation of test media.

    abstract::Most hospital bacteriologists have divided staphylococci into two groups: Staphylococcus aureus and the coagulase-negative staphylococci of which the novobiocin-resistant varieties are termed S. saprophyticus. The identification of S. aureus has been easy but that of the other staphylococci has provided some difficult...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-22-2-179

    authors: Reuther JW

    更新日期:1986-09-01 00:00:00

  • Staphylococcal whole-cell polypeptide analysis: evaluation as a taxonomic and typing tool.

    abstract::Whole-cell-polypeptide profiles obtained by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) were used in conjunction with the API-Staph technique to identify different strains of Staphylococcus aureus, S. epidermidis, S. saprophyticus and S. capitis. Complete concordance of results from both techniques was achieved ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-23-1-41

    authors: Clink J,Pennington TH

    更新日期:1987-02-01 00:00:00