Pyrolysis mass spectrometry in epidemiological and population genetic studies of Haemophilus influenzae.

Abstract:

:Haemophilus influenzae serotype b (Hib) vaccines have reduced the amount of invasive Hib disease in immunised infants. However, Hib disease remains in unvaccinated infants and adults and non-capsulate H. influenzae (NCHi) still causes infections, including outbreaks of respiratory disease. Characterisation of strains and the bacterial population as a whole is therefore necessary to detect outbreaks of infection with NCHi or changes in the population, for example, to vaccine-resistant clones of Hib. The rapid, simple and objective technique of pyrolysis mass spectrometry (PMS) was investigated as an alternative to current complex, subjective methods. PMS was compared with ribotyping and multilocus enzyme electrophoresis (MLEE) for population genetic analyses of Hib and with ribotyping and protein profiling for epidemiological analyses of NCHi. PMS clustered all the isolates of Hib together whereas MLEE and ribotyping distinguished certain clones - this is probably because the three methods examine different (and unrelated) characteristics of the organisms. The PMS results were essentially similar to those from ribotyping and protein profiling for the epidemiological analyses of outbreaks of NCHi disease. Therefore, PMS is probably unsuitable for comparisons of Hib populations but it is a useful addition to the arsenal of techniques for the characterisation of NCHi.

journal_name

J Med Microbiol

authors

Leaves NI,Sisson PR,Freeman R,Jordens JZ

doi

10.1099/00222615-46-3-204

subject

Has Abstract

pub_date

1997-03-01 00:00:00

pages

204-7

issue

3

eissn

0022-2615

issn

1473-5644

journal_volume

46

pub_type

杂志文章
  • Drug-sensitivity profile of clinical Mycobacterium tuberculosis isolates--a retrospective study from a chest-disease institute in India.

    abstract::Multi-drug-resistant tuberculosis (MDR-TB) is a major public-health problem, because treatment is complicated and patients remain infectious for months or years, despite receiving the best available therapy. To gain better understanding of MDR-TB, a retrospective study was initiated to determine the level of drug resi...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45635-0

    authors: Dam T,Isa M,Bose M

    更新日期:2005-03-01 00:00:00

  • Comparative prevalence of superantigenic toxin genes in meticillin-resistant and meticillin-susceptible Staphylococcus aureus isolates.

    abstract::A total of 118 meticillin-resistant Staphylococcus aureus (MRSA) and 140 meticillin-susceptible S. aureus (MSSA) isolates from different patients in the same time period were comprehensively searched using a multiplex PCR for the classical and recently described superantigenic toxin gene family comprising the staphylo...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.2008/002790-0

    authors: Hu DL,Omoe K,Inoue F,Kasai T,Yasujima M,Shinagawa K,Nakane A

    更新日期:2008-09-01 00:00:00

  • Alteration of pulmonary structure by Pseudomonas aeruginosa exoenzyme S.

    abstract::Intratracheal administration of purified Pseudomonas aeruginosa exoenzyme S elicited extensive, grossly observable damage in the rat lung within 2 h. Light and electronmicroscopy revealed injury and necrosis of bronchial epithelium, type I pneumocytes and capillary endothelial cells after 1 h; associated haemorrhage, ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-26-2-133

    authors: Woods DE,Hwang WS,Shahrabadi MS,Que JU

    更新日期:1988-06-01 00:00:00

  • Comparison of two matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry methods and API 20AN for identification of clinically relevant anaerobic bacteria.

    abstract::Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) is suitable for high-throughput and rapid microbial diagnosis at relatively low cost and can be considered an alternative to conventional biochemical and molecular identification systems in clinical microbiological laboratories...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.053256-0

    authors: Jamal WY,Shahin M,Rotimi VO

    更新日期:2013-04-01 00:00:00

  • Genotypic analysis of multidrug-resistant Mycobacterium tuberculosis isolates from Monterrey, Mexico.

    abstract::Thirty-seven multidrug-resistant and 13 pan-susceptible isolates of Mycobacterium tuberculosis were analysed for the diversity of genotypes associated with known drug-resistance mechanisms. The isolates were obtained from patients attending a university tuberculosis clinic in Monterrey, Mexico. A total of 25 IS6110-RF...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.05343-0

    authors: Ramaswamy SV,Dou SJ,Rendon A,Yang Z,Cave MD,Graviss EA

    更新日期:2004-02-01 00:00:00

  • Infection and tissue repair of experimental cutaneous candidiasis in diabetic mice.

    abstract:PURPOSE:Diabetic patients seem to be predisposed to cutaneous candidiasis. In this study, we evaluated the interference of diabetic conditions in alloxan-induced diabetic mice in relation to the development of C. albicans infection, density of M1 and M2 macrophages, distribution of collagen type I and III and anti-infl...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000496

    authors: Pupim ACE,Campois TG,Araújo EJA,Svidizinski TIE,Felipe I

    更新日期:2017-06-01 00:00:00

  • Nucleolar accumulation of Semliki Forest virus nucleocapsid C protein: influence of metabolic status, cytoskeleton and receptors.

    abstract::The nucleolar accumulation of Semliki Forest Virus (SFV) C protein was examined as a function of intact microtubules, intact microfilaments and accessible intermediate filaments. The cytoskeletal components do not seem to play a role in directing C protein to the nucleolus but nucleolar accumulation is energy-dependen...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-40-6-389

    authors: Jakob R

    更新日期:1994-06-01 00:00:00

  • Mycobacterium conceptionense infection complicating face rejuvenation with fat grafting.

    abstract::We report a third case of Mycobacterium conceptionense infection, which was found in a 50-year-old female following face rejuvenation surgery with fat grafting. The pathogen was identified using 16S rRNA gene and rpoB gene sequences. The growing diversity of non-tuberculosis mycobacterial species causing human infecti...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.024554-0

    authors: Yang HJ,Yim HW,Lee MY,Ko KS,Yoon HJ

    更新日期:2011-03-01 00:00:00

  • Outbreak of Klebsiella pneumoniae carbapenemase 2-producing K. pneumoniae with high qnr prevalence in a Chinese hospital.

    abstract::Forty carbapenem-resistant Klebsiella pneumoniae isolates were recovered from 28 patients from various sites in an intensive care unit in Zhejiang Provincial People's Hospital, China, over a 6 month period. PFGE analysis indicated that the 40 strains were all closely related. The MICs of carbapenems varied from 16 to ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.015826-0

    authors: Zhang R,Wang XD,Cai JC,Zhou HW,Lv HX,Hu QF,Chen GX

    更新日期:2011-07-01 00:00:00

  • Evaluation of antigenic variations between two virulent toxoplasma strains.

    abstract::Toxoplasma gondii infection in humans is routinely assessed by serological means. Here, the authors attempted to compare the response of different Toxoplasma strains to serological tests and to evaluate the antigenic profiles of the RH and RH Ankara (TRH) strains with Western blotting. Anti-Toxoplasma IgG antibodies o...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46712-0

    authors: Delibas SB,Ertabaklar H,Ertug S

    更新日期:2006-10-01 00:00:00

  • Evaluation of Albicans ID2 and Biggy agar for the isolation and direct identification of vaginal yeast isolates.

    abstract::In this study, 250 vaginal samples from patients with vulvovaginal candidosis were inoculated onto two chromogenic media, Albicans ID(2) and Biggy agar, as well as onto Sabouraud chloramphenicol agar, yielding a total of 63 yeast (25.2 %) on all three media. These strains were identified as Candida glabrata in 20 (31....

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46625-0

    authors: Ilkit M,Hilmioglu S,Tasbakan M,Aydemir S

    更新日期:2007-06-01 00:00:00

  • Prospective evaluation of BDProbeTec strand displacement amplification (SDA) system for diagnosis of tuberculosis in non-respiratory and respiratory samples.

    abstract::Nucleic acid amplification techniques (NAATs) have been demonstrated to make significant improvements in the diagnosis of tuberculosis (TB), particularly in the time to diagnosis and the diagnosis of smear-negative TB. The BD ProbeTec strand displacement amplification (SDA) system for the diagnosis of pulmonary and no...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45780-0

    authors: McHugh TD,Pope CF,Ling CL,Patel S,Billington OJ,Gosling RD,Lipman MC,Gillespie SH

    更新日期:2004-12-01 00:00:00

  • Characterisation of Eubacterium-like strains isolated from oral infections.

    abstract::The genus Eubacterium currently includes a heterogeneous group of gram-positive, non-spore-forming anaerobic bacilli, many of which are slow growing, fastidious and generally unreactive in biochemical tests. As a consequence, cultivation and identification of isolates are difficult and the taxonomy of the group remain...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-50-11-947

    authors: Downes J,Munson MA,Spratt DA,Kononen E,Tarkka E,Jousimies-Somer H,Wade WG

    更新日期:2001-11-01 00:00:00

  • Corynebacterium jeikeium pacemaker infection associated with antineutrophil cytoplasmic antibodies: a single positive blood culture could be sufficient for diagnosis.

    abstract::Corynebacterium jeikeium, a member of the non-diphtheria corynebacteria, has been rarely reported as being responsible for cardiovascular-device infection. Here, we report what is believed to be the first case of C. jeikeium pacemaker infection associated with the presence of proteinase-3 antineutrophil cytoplasmic an...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.023283-0

    authors: Bechara C,Gousseff M,Passeron A,Podglajen I,Day N,Pouchot J,Lavergne T,Mainardi JL

    更新日期:2011-02-01 00:00:00

  • Expression of prostaglandin receptors in Chlamydia trachomatis-infected recurrent spontaneous aborters.

    abstract::A study was undertaken to quantify the expression of prostaglandin (PG) receptors and find the effect of gestational age on expression of PG receptor genes in Chlamydia trachomatis-infected recurrent spontaneous aborters (RSA). Endometrial curettage tissue (ECT) was collected from 130 RSA (Group I) and 100 age-matched...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000256

    authors: Singh N,Prasad P,Singh LC,Das B,Rastogi S

    更新日期:2016-06-01 00:00:00

  • Comparative typing analyses of clinical and environmental strains of the Cryptococcus neoformans/Cryptococcus gattii species complex from Ivory Coast.

    abstract:PURPOSE:The aim of this study was to assess the biotope of the Cryptococcus neoformans/Cryptococcus gattii species complex from Ivory Coast, and clarify the possible epidemiological relationship between environmental and clinical strains. METHODOLOGY:Samples from Eucalyptus camaldulensis (n=136), Mangifera indica (n=1...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000654

    authors: Kassi FK,Bellet V,Drakulovski P,Krasteva D,Roger F,Valérie BA,Aboubakar T,Doumbia A,Kouakou GA,Delaporte E,Reynes J,Yavo W,Menan HIE,Bertout S

    更新日期:2018-01-01 00:00:00

  • A simplified system for the identification of staphylococci by multipoint inoculation of test media.

    abstract::Most hospital bacteriologists have divided staphylococci into two groups: Staphylococcus aureus and the coagulase-negative staphylococci of which the novobiocin-resistant varieties are termed S. saprophyticus. The identification of S. aureus has been easy but that of the other staphylococci has provided some difficult...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-22-2-179

    authors: Reuther JW

    更新日期:1986-09-01 00:00:00

  • Evaluation of the BD MAX Enteric Parasite Panel for the detection of Cryptosporidium parvum/hominis, Giardia duodenalis and Entamoeba histolytica.

    abstract:PURPOSE:Conventional laboratory detection methods for gastrointestinal parasites are time consuming, require considerable technical expertise and may suffer from poor analytical sensitivity. This study sought to evaluate the automated BD MAX Enteric Parasite Panel (EPP) for the detection of Cryptosporidium parvum/homin...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000558

    authors: Perry MD,Corden SA,Lewis White P

    更新日期:2017-08-01 00:00:00

  • The special affinity of particular types of Proteus mirabilis for the urinary tract.

    abstract::The strains of Proteus species found in significant numbers and as pure cultures in urine from 217 individuals were isolated, identified to species level and typed for proticine production (P type) and proticine sensitivity (S type) to give their P/S type. Urinary-tract infections with Proteus, principally P. mirabili...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-12-1-1

    authors: Senior BW

    更新日期:1979-02-01 00:00:00

  • Rapid and accurate identification of species belonging to the Candida parapsilosis complex by real-time PCR and melting curve analysis.

    abstract::Candida parapsilosis is the second most frequent Candida species isolated from blood cultures. Since 2005, C. parapsilosis has been divided into three distinct species based on genetic traits: Candida parapsilosis, Candida metapsilosis and Candida orthopsilosis. The aim of this study was to develop a rapid real-time P...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.026633-0

    authors: Hays C,Duhamel C,Cattoir V,Bonhomme J

    更新日期:2011-04-01 00:00:00

  • Acquisition of Klebsiella aerogenes by neonates.

    abstract::The acquisition of Klebsiella aerogenes by neonates born in hospital has been studied by sero- and klebecin typing. This organism was more commonly carried in the bowel of breast-fed babies than of bottle-fed babies. Only very rarely did babies acquire strains of K. aerogenes from their mothers. K. aerogenes was widel...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-12-2-201

    authors: Shinebaum R,Cooke EM,Brayson JC

    更新日期:1979-05-01 00:00:00

  • Antibiotic-loaded hydroxyapatite blocks in the treatment of experimental osteomyelitis in rats.

    abstract::A novel drug delivery system for osteomyelitis was developed in which a porous hydroxyapatite block (HAB) is loaded with antibiotic by a centrifugation method. In this study, implantation of HABs loaded with the aminoglycoside antibiotic, arbekacin, were tested in established Staphylococcus aureus osteomyelitis in the...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-46-9-779

    authors: Itokazu M,Ohno T,Tanemori T,Wada E,Kato N,Watanabe K

    更新日期:1997-09-01 00:00:00

  • Airborne transmission of lyssaviruses.

    abstract::In 2002, a Scottish bat conservationist developed a rabies-like disease and subsequently died. This was caused by infection with European bat lyssavirus 2 (EBLV-2), a virus closely related to Rabies virus (RABV). The source of this infection and the means of transmission have not yet been confirmed. In this study, the...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46370-0

    authors: Johnson N,Phillpotts R,Fooks AR

    更新日期:2006-06-01 00:00:00

  • Adhesion to and invasion of pulmonary epithelial cells by the F15/LAM4/KZN and Beijing strains of Mycobacterium tuberculosis.

    abstract::Globally, specific genotypes of Mycobacterium tuberculosis have been shown to dominate in patients, suggesting that these are more successful pathogens. One such genotype, the F15/LAM4/KZN (KZN) family of M. tuberculosis, has predominated in KwaZulu-Natal, South Africa, since the early 1990s. This strain recently evol...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.016006-0

    authors: Ashiru OT,Pillay M,Sturm AW

    更新日期:2010-05-01 00:00:00

  • Development of a routine laboratory direct detection system of staphylococcal enterotoxin genes.

    abstract::A novel direct detection system has been developed for eight staphylococcal enterotoxin (SE)-encoding genes (sea, seb, sec, sed, see, seg, seh and sei) in milk. Specific detection by real-time PCR was successful for all SE-encoding genes in the reference strains. Furthermore, a novel DNA-preparation method with good r...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46027-0

    authors: Nakayama A,Okayama A,Hashida M,Yamamoto Y,Takebe H,Ohnaka T,Tanaka T,Imai S

    更新日期:2006-03-01 00:00:00

  • Fatal post-traumatic zygomycosis in an immunocompetent young patient.

    abstract::Zygomycosis, a relatively uncommon infection, usually occurs among immunocompromised individuals. It has been reported only rarely in trauma patients. A fatal case is reported of pulmonary and rapidly progressive cutaneous zygomycosis in a young, otherwise healthy farmer, with multiple bone fractures, wounds and soft ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46993-0

    authors: Kontogiorgi M,Floros I,Koroneos A,Vamvouka C,Paniara O,Roussos C,Routsi C

    更新日期:2007-09-01 00:00:00

  • A collaborative evaluation of a rapid, semi-automated identification system for gram-negative bacilli: the Quantum II BID.

    abstract::The recently-introduced semi-automated bacterial identification system, the Quantum II BID, is designed to identify fermentative and non-fermentative gram-negative bacilli in 4-5 h. The system was evaluated independently by the two participating laboratories. Inter-laboratory reproducibility was determined by testing ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-21-2-145

    authors: Stevens M,Henrichsen C,Smith M

    更新日期:1986-03-01 00:00:00

  • A Helicobacter hepaticus catalase mutant is hypersensitive to oxidative stress and suffers increased DNA damage.

    abstract::Catalase (KatA) is known to play an important role in oxidative stress resistance in many bacterial species and a homologue exists in Helicobacter hepaticus, a member of the enterohepatic Helicobacter species. Here, a katA mutant was constructed by insertional mutagenesis and its oxidative stress phenotype was investi...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46891-0

    authors: Hong Y,Wang G,Maier RJ

    更新日期:2007-04-01 00:00:00

  • Voriconazole susceptibility of yeasts isolated from the mouths of patients with advanced cancer.

    abstract::The in vitro activity of voriconazole was compared with those of fluconazole and itraconazole against 270 clinical isolates of yeasts from the mouths of patients receiving palliative care for advanced cancer. A broth micro-dilution assay as described by the National Committee for Clinical Laboratory Standards was empl...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45720-0

    authors: Bagg J,Sweeney MP,Davies AN,Jackson MS,Brailsford S

    更新日期:2005-10-01 00:00:00

  • An update on the microbiology and epidemiology of enteropathogenic Escherichia coli in England 2010-2012.

    abstract::Historically, enteropathogenic Escherichia coli (EPEC) are a well-known cause of outbreaks of infantile diarrhoea associated with morbidity and mortality in England. The aim of this study was to provide an update on the microbiology and epidemiology of strains of EPEC in England between 2010 and 2012. A wide range of ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.062380-0

    authors: Sakkejha H,Byrne L,Lawson AJ,Jenkins C

    更新日期:2013-10-01 00:00:00