Staphylococcal whole-cell polypeptide analysis: evaluation as a taxonomic and typing tool.

abstract：:Introduction. Ocular microsporidiosis is a significant emerging infectious disease reported in immunocompromised patients and immunocompetent persons throughout the world.Aim. To identify the pathogens responsible for human keratitis, via corneal scrapings.Methodology. Thirty-three hospitalized patients with epithelia...

journal_title：Journal of medical microbiology

authors： Sanpool O,Thanathanee O,Laummuanwai P,Maleewong W,Intapan PM

更新日期：2020-03-01 00:00:00

abstract：:A pathogenic vancomycin-resistant Staphylococcus aureus (VRSA) isolate (MIC > or =64 microg ml(-1)) was obtained from a Kolkata hospital in June 2005. Species identification was confirmed by Gram staining, standard biochemical tests and PCR amplification of the nuc gene, which encodes the thermostable nuclease that is...

journal_title：Journal of medical microbiology

authors： Saha B,Singh AK,Ghosh A,Bal M

更新日期：2008-01-01 00:00:00

abstract：PURPOSE:Strains of type emm89 Streptococcus pyogenes have recently increased in frequency as a cause of human infections in several countries in Europe and North America. This increase has been molecular epidemiologically linked with the emergence of a new genetically distinct clone, designated clade 3. We sought to ex...

journal_title：Journal of medical microbiology

authors： Beres SB,Olsen RJ,Ojeda Saavedra M,Ure R,Reynolds A,Lindsay DSJ,Smith AJ,Musser JM

更新日期：2017-12-01 00:00:00

abstract：:Subtractive hybridisation was used to screen for and identify conserved DNA sequences associated with clinical, clonal populations of Staphylococcus aureus. DNA from S. aureus strain ISP8 (a clinical isolate) was digested with TaqI and hybridised with randomly fragmented pooled DNA from 10 non-clinical (community) iso...

journal_title：Journal of medical microbiology

authors： el-Adhami WA,Stewart PR,Matthaei KI

更新日期：1997-12-01 00:00:00

abstract：:The effects of ethyleneglycoltetra-acetic acid (EGTA) and EGTA + magnesium (MgEGTA) on the viable counts of 10 strains of Escherichia coli O6 have been studied in normal human serum (NHS), heat-inactivated serum (HIS) and in culture media with and without the addition of a beta-lactam antibiotic. The addition of EGTA ...

journal_title：Journal of medical microbiology

authors： Roberts AP,Phillips R

更新日期：1981-05-01 00:00:00

abstract：:We report a case of septicaemia with prolonged, refractory hypotension related to Moraxella osloensis isolated in a non-immunocompromised paediatric patient. ...

journal_title：Journal of medical microbiology

authors： Dien Bard J,Lewinski M,Summanen PH,Deville JG

更新日期：2011-01-01 00:00:00

abstract：:Yeast identification and in vitro susceptibility testing provide helpful information for appropriate administration of antifungal treatments; however, few reports from the Latin American region have been published. The aim of this study was to identify the species present in isolates from bloodstream infections diagno...

journal_title：Journal of medical microbiology

authors： Bustamante B,Martins MA,Bonfietti LX,Szeszs MW,Jacobs J,Garcia C,Melhem MSC

更新日期：2014-06-01 00:00:00

abstract：:The family of leptospiral immunoglobulin-like (lig) genes comprises ligA, ligB and ligC. This study used PCR to demonstrate the presence of lig genes among serovars from a collection of leptospiral strains and clinical isolates. Whilst ligA and ligC appeared to be present in a limited number of pathogenic serovars, th...

journal_title：Journal of medical microbiology

authors： Cerqueira GM,McBride AJA,Picardeau M,Ribeiro SG,Moreira ÂN,Morel V,Reis MG,Ko AI,Dellagostin OA

更新日期：2009-09-01 00:00:00

abstract：PURPOSE:Non-albicansCandida species have emerged as fungal pathogens that cause invasive infections, with many of these species displaying resistance to commonly used antifungal agents. This study was confined to studying the characteristics of clinical isolates of the C. rugosa complex and C. pararugosa species. METH...

journal_title：Journal of medical microbiology

authors： Peremalo T,Madhavan P,Hamzah S,Than L,Wong EH,Nasir MDM,Chong PP,Ng KP

更新日期：2019-03-01 00:00:00

abstract：:Rhodococcus equi is one of the most important causes of pneumonia in foals and has emerged as a significant opportunistic pathogen of immunosuppressed hosts such as human immunodeficiency virus-infected patients. Virulent R. equi harbouring an 85 kb plasmid, but not the avirulent form lacking the plasmid, has the abil...

journal_title：Journal of medical microbiology

authors： Toyooka K,Takai S,Kirikae T

更新日期：2005-11-01 00:00:00

abstract：:The move towards pathology networks and hub-and-spoke models of medical laboratory service provision has significantly changed the flow of samples, and the impact of results on patients, over recent years. At the same time advances in technology, including rapid, simple to use molecular platforms, are changing the way...

journal_title：Journal of medical microbiology

authors： Rabinowicz S,O'Hare M,Moore LSP,Mughal N

更新日期：2019-03-01 00:00:00

abstract：:A polymerase chain reaction (PCR) assay with oligonucleotide primers homologous to a portion of the urease C gene of Helicobacter pylori was evaluated for specificity with pure DNA and biopsy material. The assay was used to test for the presence of the organism in dental plaque. The species specificity of detection wa...

journal_title：Journal of medical microbiology

authors： Bickley J,Owen RJ,Fraser AG,Pounder RE

更新日期：1993-11-01 00:00:00

abstract：:Pyrolysis mass spectrometry (PyMS), a highly discriminatory method for comparison of isolates, was used to assess the homogeneity of colonies taken from apparently pure cultures of Escherichia coli in 10 urine specimens. Ten randomly selected colonies were subcultured from each urine for comparison. For six urines, th...

journal_title：Journal of medical microbiology

authors： Freeman R,Sisson PR,Burdess D

更新日期：1996-11-01 00:00:00

abstract：:Extracts of 19 samples of pus which showed red fluorescence with ultraviolet light were screened for the presence of porphyrins by absorption spectrophotometry. All those which showed spectra typical of metal-free porphyrins were analysed by high performance liquid chromatography to identify the porphyrins present. Th...

journal_title：Journal of medical microbiology

authors： Brazier JS

更新日期：1990-09-01 00:00:00

abstract：:Two types of fimbriae, designated aggregative adherence fimbria I and II (AAF/I and AAF/II) have been described in enteroaggregative Escherichia coli (EAEC) strains. These fimbriae mediate the aggregative pattern of adherence (AA) to epithelial cells. The genes encoding the structural subunit of each fimbria have been...

journal_title：Journal of medical microbiology

authors： Elias WP,Suzart S,Trabulsi LR,Nataro JP,Gomes TAT

更新日期：1999-06-01 00:00:00

abstract：:In tests of bactericidal action against H. influenzae type b strains isolated from patients with meningitis, chloramphenicol was found to be far more reliable than ampicillin in dealing with large inocula, and more rapidly effective against both large and relatively small inocula. These findings provide a laboratory e...

journal_title：Journal of medical microbiology

authors： Turk DC

更新日期：1977-02-01 00:00:00

abstract：:Acinetobacter junii is a rare human pathogen associated with bacteraemia in neonates and paediatric oncology patients. We present a case of A. junii causing bacteraemia in an adult transplant patient with leukaemia. The correct identification of Acinetobacter species can highlight the clinical significance of the diff...

journal_title：Journal of medical microbiology

authors： Cayô R,Yañez San Segundo L,Pérez Del Molino Bernal IC,García de la Fuente C,Bermúdez Rodríguez MA,Calvo J,Martínez-Martínez L

更新日期：2011-03-01 00:00:00

abstract：PURPOSE:Pertussis remains a public health concern in most countries. Our study aimed to prospectively explore the epidemiology of pertussis in the Tunis area of Tunisia between 2007 and 2016, and to characterize the virulence-associated genes of the collected Bordetella pertussis isolates. METHODOLOGY:Infants and chil...

journal_title：Journal of medical microbiology

authors： Ben Fraj I,Kechrid A,Guillot S,Bouchez V,Brisse S,Guiso N,Smaoui H

更新日期：2019-02-01 00:00:00

abstract：:Chlamydia trachomatis results in tubal factor infertility in some women. Diagnosis of this tubal infertility is difficult and typically involves laparoscopy or hysterosalpingography to detect the tubal blockages. Numerous serological tests have been developed; however, they are presently not used for diagnosis without...

journal_title：Journal of medical microbiology

authors： Menon S,Stansfield SH,Logan B,Hocking JS,Timms P,Rombauts L,Allan JA,Huston WM

更新日期：2016-09-01 00:00:00

abstract：:Bedaquiline (BDQ) has been proven to be effective in the treatment of multidrug-resistant tuberculosis. We hypothesized that BDQ could be a potential agent to treat nontuberculous mycobacterial (NTM) infection. The objective of this study was to evaluate the in vitro activity of BDQ against rapidly growing mycobacteri...

journal_title：Journal of medical microbiology

authors： Aguilar-Ayala DA,Cnockaert M,André E,Andries K,Gonzalez-Y-Merchand JA,Vandamme P,Palomino JC,Martin A

更新日期：2017-08-01 00:00:00

abstract：PURPOSE:This paper aims to evaluate the antimicrobial resistance of Esherichia coli isolates from children under 5 years old, with and without diarrhoea, who were hospital outpatients in Culiacan, Sinaloa, Mexico. It also looks at the antimicrobial activity of fruit extracts against selected multidrug-resistant (MDR) E...

journal_title：Journal of medical microbiology

authors： Uribe-Beltrán MJ,Ahumada-Santos YP,Díaz-Camacho SP,Eslava-Campos CA,Reyes-Valenzuela JE,Báez-Flores ME,Osuna-Ramírez I,Delgado-Vargas F

更新日期：2017-07-01 00:00:00

abstract：:We sought evidence to determine if particular strains of Pseudomonas aeruginosa have a predilection for pulmonary colonisation in patients with cystic fibrosis (CF). The incidence of common pyocin types in non-CF isolates (74%) was similar to that noted in previous reports but differed significantly (X2 = 16.7, p less...

journal_title：Journal of medical microbiology

authors： Tredgett MW,Doherty C,Govan JR

更新日期：1990-07-01 00:00:00

abstract：:Epidemic methicillin-resistant Staphylococcus aureus types 15 and 16 (EMRSA-15 and EMRSA-16) are the dominant types of MRSA found in UK hospitals, but accurate designation of strains has been difficult. Restriction fragment length polymorphism (RFLP) profiles of seven core virulence genes were used to classify unambig...

journal_title：Journal of medical microbiology

authors： Moore PCL,Lindsay JA

更新日期：2002-06-01 00:00:00

abstract：:The biochemical characteristics, enterotoxigenicity and susceptibility to antibiotics are reported for 22 strains of Aeromonas species isolated from clinical specimens in the Western Region of Saudi Arabia. Aeromonas caviae was the species most frequently observed; a high proportion of these strains fermented lactose,...

journal_title：Journal of medical microbiology

authors： Gosling PJ

更新日期：1986-08-01 00:00:00

abstract：:Blood cultures are sometimes obtained from intravascular catheters for convenience. However, there is controversy regarding this practice. The authors compared the diagnostic test characteristics of blood cultures obtained from intravascular catheters and peripheral veins. Relevant studies for inclusion in this review...

journal_title：Journal of medical microbiology

authors： Falagas ME,Kazantzi MS,Bliziotis IA

更新日期：2008-01-01 00:00:00

abstract：:There is great interest in the use of antimicrobial agents for the prevention and treatment of plaque-related oral diseases and many publications have reported the results of studies in which the minimum inhibitory concentrations of agents for cariogenic and periodontopathogenic bacteria have been determined. However,...

journal_title：Journal of medical microbiology

authors： Wilson M

更新日期：1996-02-01 00:00:00

abstract：:A mouse enterohaemorrhagic Escherichia coli (EHEC) infection model was developed with a combination of germ-free (GF) mice and hyper-toxigenic EHEC (HT-EHEC) O157:H7 strain 6. The HT-EHEC strain 6 produced both Shiga-like toxin (SLT)-1 1.0 microg/mI and SLT-2 8.2 microg/ml in vitro. Eight-week-old germ-free mice were ...

journal_title：Journal of medical microbiology

authors： Taguchi H,Takahashi M,Yamaguchi H,Osaki T,Komatsu A,Fujioka Y,Kamiya S

更新日期：2002-04-01 00:00:00

abstract：PURPOSE:Listeria monocytogenes is a genetically heterogeneous species, which is divided into evolutionary lineages and clonal complexes (CCs). Not all L. monocytogenes isolates are equally likely to cause disease, with CC1, and in particular sequence type (ST) 1, being the most prevalent complex in human and ruminant i...

journal_title：Journal of medical microbiology

authors： Rupp S,Bärtschi M,Frey J,Oevermann A

更新日期：2017-07-01 00:00:00

abstract：:Clostridium perfringens has been reported as the cause of up to 15% of cases of antibiotic-associated diarrhoea (AAD) and may be diagnosed by detection of enterotoxin (CPEnt) in faeces. The performance of a commercial ELISA method for CPEnt, with culture and PCR methods to confirm the presence of enterotoxigenic C. pe...

journal_title：Journal of medical microbiology

authors： Asha NJ,Wilcox MH

更新日期：2002-10-01 00:00:00

abstract：:Aminoglycoside-resistant variants of Pseudomonas aeruginosa strain PAO1 were readily selected by culturing the organism in medium containing increasing concentrations of gentamicin, tobramycin or amikacin until the strains were growing in a concentration of drug 128-fold greater than the minimal inhibitory concentrati...

journal_title：Journal of medical microbiology

authors： Gilleland LB,Gilleland HE,Gibson JA,Champlin FR

更新日期：1989-05-01 00:00:00