Abstract:
:Pyrolysis mass spectrometry (PyMS), a highly discriminatory method for comparison of isolates, was used to assess the homogeneity of colonies taken from apparently pure cultures of Escherichia coli in 10 urine specimens. Ten randomly selected colonies were subcultured from each urine for comparison. For six urines, the set of 10 single-colony isolates proved indistinguishable by PyMS. However, for four urines there was clear heterogeneity. For two urines, one of the isolates was distinct, and the remaining nine indistinguishable. One urine yielded seven indistinguishable isolates, and three further individually distinct isolates. One urine yielded two clusters of indistinguishable isolates, one comprising seven and the other three isolates. Thus, significant heterogeneity (as measured by PyMS) occurs in apparently pure cultures of E. coli from urinary tract infection. The nature of this heterogeneity remains to be established, as does the significance, if any, of this phenomenon in urinary tract infection.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Freeman R,Sisson PR,Burdess Ddoi
10.1099/00222615-45-5-349subject
Has Abstractpub_date
1996-11-01 00:00:00pages
349-52issue
5eissn
0022-2615issn
1473-5644journal_volume
45pub_type
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更新日期:2017-04-01 00:00:00
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更新日期:2010-01-01 00:00:00
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journal_title:Journal of medical microbiology
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更新日期:2005-05-01 00:00:00
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journal_title:Journal of medical microbiology
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journal_title:Journal of medical microbiology
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更新日期:1998-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1996-12-01 00:00:00