Abstract:
:Aminoglycoside-resistant variants of Pseudomonas aeruginosa strain PAO1 were readily selected by culturing the organism in medium containing increasing concentrations of gentamicin, tobramycin or amikacin until the strains were growing in a concentration of drug 128-fold greater than the minimal inhibitory concentration for the sensitive parent strain. These resistant strains exhibited characteristics previously associated with the impermeability type of resistance mechanism, i.e., they grew more slowly than the parent strain, the resistance was unstable in the absence of the antibiotic, and adaptation to one of the antibiotics conferred cross-resistance to other aminoglycosides. The adapted strains grew, with minimal morphological alterations, in concentrations of the various aminoglycosides that normally produced cell envelope damage, misshapen and filamentous cell formation, and cell lysis in the sensitive strain. Neither protein H1 nor phospholipid alterations appear to play a significant role in adaptive resistance to aminoglycoside antibiotics in this model system. The acquisition of adaptive resistance to the aminoglycoside antibiotics did not confer resistance to polymyxin B, another cationic antibiotic which is thought to share binding sites within the outer membrane with the aminoglycosides.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Gilleland LB,Gilleland HE,Gibson JA,Champlin FRdoi
10.1099/00222615-29-1-41subject
Has Abstractpub_date
1989-05-01 00:00:00pages
41-50issue
1eissn
0022-2615issn
1473-5644journal_volume
29pub_type
杂志文章abstract::To gain a better understanding of the pathological role of interferon-gamma (IFN-gamma) in specific granuloma formation, IFN-gamma gene-deficient mice (BALB/c and C57BL/6) were produced. The IFN-gamma gene in embryonic stem (ES) cells was disrupted by inserting the beta-galactosidase gene (lacZ) and the neomycin resis...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-47-10-871
更新日期:1998-10-01 00:00:00
abstract::Motile curved rods seen in vaginal secretions have been isolated on Columbia agar supplemented with 5% human blood and vitamin K. Growth occurred anaerobically and in 5% oxygen but not in more aerobic conditions. There were two distinct groups of these organisms, distinguishable by morphology, biochemical activity and...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-16-2-175
更新日期:1983-05-01 00:00:00
abstract:PURPOSE:Diabetic patients seem to be predisposed to cutaneous candidiasis. In this study, we evaluated the interference of diabetic conditions in alloxan-induced diabetic mice in relation to the development of C. albicans infection, density of M1 and M2 macrophages, distribution of collagen type I and III and anti-infl...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000496
更新日期:2017-06-01 00:00:00
abstract::The National Institute of Care Excellence (NICE) 2007 guidance CG54, on urinary tract infection (UTI) in children, states that clinicians should use urgent microscopy and culture as the preferred method for diagnosing UTI in the hospital setting for severe illness in children under 3 years old and from the GP setting ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000114
更新日期:2015-09-01 00:00:00
abstract::The morbidity and mortality rates in patients with cystic fibrosis (CF) are significantly affected by infections with Burkholderia cepacia complex. In a Czech CF Centre, the prevalence of the infection reached up to 30 %, with the majority of patients found to be infected with Burkholderia cenocepacia (formerly genomo...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46025-0
更新日期:2005-07-01 00:00:00
abstract::An evaluation of five ELISA methods for the diagnosis of acute toxoplasmosis was undertaken by comparing a laboratory-produced ELISA employing two recombinant Toxoplasma gondii polypeptides as antigen with a laboratory-produced ELISA and three commercially available ELISAs employing traditional parasite antigen prepar...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-37-6-404
更新日期:1992-12-01 00:00:00
abstract::This study attempted to determine whether cefuroxime was superior to cephalothin as a surrogate marker for cefpodoxime among urinary tract isolates. The MicroScan system (Siemens) was used to determine susceptibility for cephalothin and cefuroxime on consecutive cultures with a colony count of ≥50 000 organisms. Simul...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.063040-0
更新日期:2014-02-01 00:00:00
abstract::Blood cultures are sometimes obtained from intravascular catheters for convenience. However, there is controversy regarding this practice. The authors compared the diagnostic test characteristics of blood cultures obtained from intravascular catheters and peripheral veins. Relevant studies for inclusion in this review...
journal_title:Journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.1099/jmm.0.47432-0
更新日期:2008-01-01 00:00:00
abstract::The aim of this study was to evaluate an enzyme-linked immunosorbent assay (ELISA) for the detection of anti-Helicobacter pylori specific IgG antibodies in specimens of oral fluid. Antral biopsy specimens, serum and oral fluid samples were collected from 81 patients attending for upper gastrointestinal endoscopy. The ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-48-11-1043
更新日期:1999-11-01 00:00:00
abstract:PURPOSE:The analytical performance of the cobas 6800 HIV-1, HBV and HCV assays was verified and evaluated to the COBAS Ampliprep/COBAS TaqMan assays. METHODOLOGY:The precision, limit of detection (LoD), limit of quantification (LoQ) and linearity were verified using pooled residual clinical samples. The analytical spe...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000838
更新日期:2018-12-01 00:00:00
abstract::Intravenous injection of eight human strains of Campylobacter fetus ss fetus and Campylobacter jejuni into mice at various stages of pregnancy demonstrated significant strain differences in ability to affect implantation of the fertilised ovum and to cause resorption of the mouse fetus. Implantation was significantly ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-25-1-7
更新日期:1988-01-01 00:00:00
abstract::Introduction.Candida auris is a pathogenic yeast that mainly affects immunosuppressed patients and those with implanted medical devices. This pathogen also displays elevated resistance to common antifungals and high survival and spreading capacities. Since no antifungal breakpoints have yet been defined for this patho...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.001036
更新日期:2019-09-01 00:00:00
abstract::The objectives of the present study were to evaluate in vitro the production of antagonistic compounds against Gardnerella vaginalis by Lactobacillus strains isolated from women with or without bacterial vaginosis (BV), and to select one of the better Lactobacillus producers of such a substance to be tested in vivo us...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.041962-0
更新日期:2012-08-01 00:00:00
abstract::Seven isolates of B. piliformis, the agent of Tyzzer's disease, obtained from various host species, were examined for cytotoxic activity by incubating culture filtrates on BRL 3A rat-hepatocyte and 3T3 mouse-fibroblast cell lines. One isolate exhibited cytopathic effects on BRL 3A cells, but not on 3T3 cells. Three ot...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-37-1-77
更新日期:1992-07-01 00:00:00
abstract::Introduction. PCV2 is a DNA virus that exists widely in pigs and has caused great economic losses to the pig industry worldwide. In the existing commercial PCV2 enzyme-linked immunosorbent assay (ELISA) kits both natural infection with PCV2 and vaccine immunization produce results that are positive for PCV2 Cap antibo...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.001230
更新日期:2020-09-01 00:00:00
abstract::Lactulose exerts a beneficial effect on hepatic encephalopathy by decreasing toxic short-chain (iC4-nC6) fatty acid (isobutyrate, butyrate, isovalerate, valerate, isocaproate and caproate) production. However, the precise mechanism by which lactulose exerts this effect remains uncertain. This study investigated the ef...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-46-1-80
更新日期:1997-01-01 00:00:00
abstract::A total of 69 strains of Escherichia coli from patients in the Taizhou Municipal Hospital, China, were isolated, and 11 strains were identified that were resistant to bacitracin, chloramphenicol, tetracycline and erythromycin. These strains were PCR positive for at least two out of three genes, ybjG, dacC and mdfA, by...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.062893-0
更新日期:2013-11-01 00:00:00
abstract::Meticillin-resistant Staphylococcus aureus (MRSA) was unexpectedly isolated from a pig used for streptozotocin-induced diabetes research. To investigate the possible source of the MRSA isolate, nasal swabs were obtained from the animal herd, and from animal holding rooms, and veterinary and research staff involved in ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.47283-0
更新日期:2007-08-01 00:00:00
abstract::Rhodococcus equi is an opportunistic human pathogen associated with immunosuppressed people. While the interaction of R. equi with macrophages has been comprehensively studied, little is known about its interactions with non-phagocytic cells. Here, we characterized the entry process of this bacterium into human lung e...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.056234-0
更新日期:2013-08-01 00:00:00
abstract::Pulsed-field gel electrophoresis typing was performed on a retrospective set of 129 Moraxella catarrhalis isolates obtained over a 20 month period from 70 children admitted to, or presenting at, the Erasmus University Medical Center, Rotterdam, The Netherlands. The mean age of the children (at the end of the study) wa...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.05251-0
更新日期:2003-09-01 00:00:00
abstract::Over the last 30 years, Serratia marcescens has become an important cause of nosocomial infection. There have been many reports concerning the identification, antibiotic susceptibility, pathogenicity, epidemiological investigations and typing of this organism. Accurate identification is important in defining outbreaks...
journal_title:Journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.1099/00222615-46-11-903
更新日期:1997-11-01 00:00:00
abstract::Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) is suitable for high-throughput and rapid microbial diagnosis at relatively low cost and can be considered an alternative to conventional biochemical and molecular identification systems in clinical microbiological laboratories...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.053256-0
更新日期:2013-04-01 00:00:00
abstract::Campylobacter strains isolated from the same stool sample were characterised by determination of biochemical properties and both heat-labile (Lior) and heat-stable (Lauwers) serotypes. In six of 60 campylobacter-infected stools, two or three strains differing in Lior-serotype were isolated from the same stool. In four...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-38-1-3
更新日期:1993-01-01 00:00:00
abstract::Herpes simplex virus type-1 (HSV-1) induces Fc- and C3b(i)-receptors on infected cells. The role of these receptors in bacterial superinfection was studied by comparing the adherence of non-opsonised and opsonised bacteria to HSV-infected and non-infected HEp-2 cells. A flow cytometric adherence assay, based on the fl...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-40-1-48
更新日期:1994-01-01 00:00:00
abstract::A novel drug delivery system for osteomyelitis was developed in which a porous hydroxyapatite block (HAB) is loaded with antibiotic by a centrifugation method. In this study, implantation of HABs loaded with the aminoglycoside antibiotic, arbekacin, were tested in established Staphylococcus aureus osteomyelitis in the...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-46-9-779
更新日期:1997-09-01 00:00:00
abstract::The strains of Proteus species found in significant numbers and as pure cultures in urine from 217 individuals were isolated, identified to species level and typed for proticine production (P type) and proticine sensitivity (S type) to give their P/S type. Urinary-tract infections with Proteus, principally P. mirabili...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-12-1-1
更新日期:1979-02-01 00:00:00
abstract::Erythrocyte-sensitising antigens (AE) were prepared from Vibrio cholerae serotypes, from EL-Tor vibrio, Escherichia coli and Salmonella enteritidis by digesting the organisms with NaOH followed by precipitation with alcohol. When AE was used in indirect haemagglutination (IHA) tests, the results in a number of cases w...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-8-1-11
更新日期:1975-02-01 00:00:00
abstract::Acute hypercytokinaemia represents an imbalance of pro-inflammatory and anti-inflammatory cytokines, and is believed to be responsible for the development of acute respiratory distress syndrome and multiple organ failure in severe cases of avian (H5N1) influenza. Although neuraminidase inhibitors are effective in trea...
journal_title:Journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.1099/jmm.0.47124-0
更新日期:2007-07-01 00:00:00
abstract::The antifungal susceptibilities of 598 isolates of Candida spp. (bloodstream and other sterile sites) to liposomal amphotericin B (L-AmB) versus amphotericin B (AmB) were determined. MICs were calculated using the Clinical and Laboratory Standards Institute broth microdilution (M27-A3) method for L-AmB and the Etest m...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000402
更新日期:2017-03-01 00:00:00
abstract::Neisseria gonorrhoeae expresses receptors for both lactoferrin (LF) and transferrin (TF). To determine whether qualitative or quantitative changes in these receptors, or both, correlate with the pattern of disease due to N. gonorrhoeae, a dot binding assay with whole cells was used to measure the absolute receptor lev...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-28-3-199
更新日期:1989-03-01 00:00:00