Abstract：
:The aim of this study was to evaluate an enzyme-linked immunosorbent assay (ELISA) for the detection of anti-Helicobacter pylori specific IgG antibodies in specimens of oral fluid. Antral biopsy specimens, serum and oral fluid samples were collected from 81 patients attending for upper gastrointestinal endoscopy. The presence or absence of current H. pylori infection was determined by culture, histology and urease detection. Anti-H. Pylori specific IgG was detected in serum by an established in-house ELISA and in oral fluid by an ELISA developed for this study. In all, 34 (42%) of 81 patients were positive for H. pylori by one or more of the 'gold standard' tests (culture, histology and urease detection). The oral fluid ELISA had a sensitivity of 94% and specificity of 85% with regard to current H. pylori infection. The serum ELISA had a sensitivity and specificity of 91%. There was an overall agreement of 88% between serum and oral fluid antibody detection. The detection of anti-H. pylori specific IgG in oral fluid by ELISA is comparable in sensitivity and specificity with serum-based methods. Oral fluid-based ELISA could provide a reliable, non-invasive method for the diagnosis of H. pylori infection, and may be of particular benefit for population surveys.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Marshall B,Howat AJ,Wright PAdoi
10.1099/00222615-48-11-1043keywords:
subject
Has Abstractpub_date
1999-11-01 00:00:00pages
1043-1046issue
11eissn
0022-2615issn
1473-5644journal_volume
48pub_type
杂志文章abstract：:Clostridium perfringens has been reported as the cause of up to 15% of cases of antibiotic-associated diarrhoea (AAD) and may be diagnosed by detection of enterotoxin (CPEnt) in faeces. The performance of a commercial ELISA method for CPEnt, with culture and PCR methods to confirm the presence of enterotoxigenic C. pe...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/0022-1317-51-10-891
更新日期：2002-10-01 00:00:00
abstract：:Leclercia adecarboxylata is a rarely reported human pathogen, most commonly affecting immunocompromised individuals. In reported cases of immunocompetent patients infected with this organism, it is seen exclusively in the context of polymicrobial infections. We report here the case of an abscess in an immunocompetent ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.47673-0
更新日期：2008-07-01 00:00:00
abstract：:Clostridium difficile-associated diarrhoea (CDAD) is the most common hospital-acquired diarrhoea, and is a major type of gastroenteritis infection in nursing homes and facilities for the elderly. In this study the antimicrobial activity of the two-component lantibiotic, lacticin 3,147, against a range of genetically d...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.47085-0
更新日期：2007-07-01 00:00:00
abstract：:The cystic fibrosis (CF) airways are colonized by polymicrobial communities with high bacterial load and are influenced by frequent antibiotic exposures. This community includes diverse streptococci, some of which have been directly or indirectly associated with pulmonary exacerbations. As many streptococci are natura...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000172
更新日期：2015-11-01 00:00:00
abstract：:The strains of Proteus species found in significant numbers and as pure cultures in urine from 217 individuals were isolated, identified to species level and typed for proticine production (P type) and proticine sensitivity (S type) to give their P/S type. Urinary-tract infections with Proteus, principally P. mirabili...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-12-1-1
更新日期：1979-02-01 00:00:00
abstract：:Isolates of Vibrio cholerae O1 biotype El Tor serotype Inaba associated with an outbreak of cholera in Trivandrum, southern India, were characterized. PCR testing revealed that all five isolates examined carried the TCP pathogenicity island, the CTX genetic element and the RTX toxin, and produced cholera toxin (CT). R...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.46868-0
更新日期：2007-02-01 00:00:00
abstract：:To gain insight into the value of lipopolysaccharide (LPS) core determinants for cross-protective immunisation the serological relationships between six complete (LPS) core types from Enterobacteriaceae were investigated. Hyperimmune sera were raised in mice by repeated immunisation with heat-killed strains of Salmone...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-48-5-433
更新日期：1999-05-01 00:00:00
abstract：:We report a case of septicaemia with prolonged, refractory hypotension related to Moraxella osloensis isolated in a non-immunocompromised paediatric patient. ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.016378-0
更新日期：2011-01-01 00:00:00
abstract：:A total of 118 meticillin-resistant Staphylococcus aureus (MRSA) and 140 meticillin-susceptible S. aureus (MSSA) isolates from different patients in the same time period were comprehensively searched using a multiplex PCR for the classical and recently described superantigenic toxin gene family comprising the staphylo...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.2008/002790-0
更新日期：2008-09-01 00:00:00
abstract：:PCR-based detection of Mucorales species could improve diagnosis of suspected invasive fungal infection, leading to a better patient outcome. This study describes two independent probe-based real-time PCR tests for detection of clinically relevant Mucorales, targeting specific fragments of the 18S and the 28S rRNA gen...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000218
更新日期：2016-03-01 00:00:00
abstract：:A total of 1878 non-duplicate clinical Escherichia coli isolates (comprising 1711 urinary isolates and 167 blood-culture isolates), which were collected from multiple centres in Hong Kong during 1996-2008, were used to investigate the prevalence and molecular epidemiology of plasmid-mediated fosfomycin (fos) resistanc...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.062653-0
更新日期：2013-11-01 00:00:00
abstract：:Listeria monocytogenes cells are ingested by protozoa such as Acanthamoeba sp. or Tetrahymena pyriformis. However, they are not killed, but survive within the protozoa and may multiply intracellularly. The protozoa are lysed within about 8 days, releasing viable L. monocytogenes. No co-existence was observed between L...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-33-1-51
更新日期：1990-09-01 00:00:00
abstract：:The distribution of hepatitis C virus (HCV) genotypes among Egyptian patients positive for anti-HCV was determined and their influence, when combined with neu-oncoprotein overexpression, on the development of hepatocellular carcinoma (HCC) was examined. The study groups included asymptomatic carriers (ASC) and patient...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/0022-1317-49-1-89
更新日期：2000-01-01 00:00:00
abstract：:An affinity procedure with purified, biotinylated human transferrin and streptavidin-agarose was used to identify the transferrin-binding proteins in strains of Haemophilus influenzae. Proteins of 58 and 98 Kda were isolated from total membranes prepared from iron-deficient but not iron-sufficient H. influenzae KC548 ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-29-2-121
更新日期：1989-06-01 00:00:00
abstract：:In this study, the species diversity of staphylococci with inducible resistance to macrolides, lincosamides and streptogramin B (MLSB) isolated from clinical samples, sewage and river water was investigated. Inducible clindamycin resistance was tested using a D-test and macrodilution assays. Inducible cross-resistance...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.077081-0
更新日期：2014-11-01 00:00:00
abstract：:An ammonium chloride procedure was used to prepare a bacterial pellet from positive blood cultures, which was used for direct inoculation of VITEK 2 cards. Correct identification reached 99% for Enterobacteriaceae and 74% for staphylococci. For antibiotic susceptibility testing, very major and major errors were 0.1 an...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.049361-0
更新日期：2013-05-01 00:00:00
abstract：:Bacterial infection in patients who have undergone liver transplantation is a major complication of the procedure. Leuconostoc spp. are important pathogenic bacteria in individuals with poor immune function, especially transplant patients. In this report, we describe the case of a 45-year-old Asian male liver transpla...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.045161-0
更新日期：2012-11-01 00:00:00
abstract：:Recent studies have shown that the intestinal microbiota is essential for the pathogenicity but not for the multiplication of Giardia duodenalis in the intestinal lumen. The microbial components responsible for this phenomenon are not known. Twenty-eight facultative and three strictly anaerobic micro-organisms were is...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/0022-1317-49-3-209
更新日期：2000-03-01 00:00:00
abstract：:CaNdt80p, the Candida albicans homologue of the Saccharomyces cerevisiae transcription factor ScNdt80p, has been identified as a positive regulator of CDR1, which encodes an efflux pump involved in drug resistance in C. albicans. To investigate the involvement of the putative DNA-binding domain of CaNdt80p in drug res...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.46650-0
更新日期：2006-10-01 00:00:00
abstract：PURPOSE:Extensively drug-resistant (XDR) strains of Acinetobacter baumannii are being reported worldwide, and they are associated with high morbidity and mortality rates. These strains are considered to be the highest priority for the development of new antibacterial agents. Therefore, we aimed to develop an effective ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.001002
更新日期：2019-07-01 00:00:00
abstract：:Since 2007, there has been a re-emergence of cholera outbreaks in northern Vietnam. To understand the molecular epidemiological relatedness and determine the antibiotic susceptibility profiles of responsible V. cholerae O1 outbreak strains, a representative collection of 100 V. cholerae O1 strains was characterized. V...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.034744-0
更新日期：2012-03-01 00:00:00
abstract：:Two monoclonal antibodies (MAbs) to a 36-kDa extracellular metalloprotease (PSCP) from Burkholderia (Pseudomonas) cepacia were found to react with thermolysin, Pseudomonas aeruginosa elastase, alkaline protease (Apr) and LasA, Serratia marcescens protease (SMP), Aeromonas hydrophila protease (AhP), and both the lethal...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-45-3-219
更新日期：1996-09-01 00:00:00
abstract：:The ability of rabbits to clear an intraperitoneal injection of Pseudomonas aeruginosa in the presence or absence of a surgically implanted peritoneal device was investigated. Sham-operated rabbits without an implant eliminated a P. aeruginosa challenge of 5 x 10(6) cfu/ml; lavage fluid and peritoneal tissues became c...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-36-6-406
更新日期：1992-06-01 00:00:00
abstract：:Leptospirosis is a widespread zoonotic disease that affects all mammals in different parts of the world. Though there are many commercial kits available for the diagnosis of systemic leptospirosis, the nature of the antigen has not been described. Therefore, identification of a specific antigen is important. Since ocu...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.05120-0
更新日期：2003-08-01 00:00:00
abstract：:Bacteroides gracilis is a gram-negative anaerobic bacillus which requires formate and fumarate for growth; it has been implicated in periodontal disease and serious infections of the head and neck. In this study, Bacteroides ureolyticus (NTU) medium was tested for its ability to allow the growth of B. gracilis and oth...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-35-5-294
更新日期：1991-11-01 00:00:00
abstract：:Acute hypercytokinaemia represents an imbalance of pro-inflammatory and anti-inflammatory cytokines, and is believed to be responsible for the development of acute respiratory distress syndrome and multiple organ failure in severe cases of avian (H5N1) influenza. Although neuraminidase inhibitors are effective in trea...
journal_title：Journal of medical microbiology
pub_type： 杂志文章,评审
doi：10.1099/jmm.0.47124-0
更新日期：2007-07-01 00:00:00
abstract：:Antibodies specific for rubella virion polypeptide, VPI, secreted by clones of hybridoma cells or produced in rabbits in response to specific antigenic stimulation, located determinants for haemagglutination inhibiting (HI) and neutralising (Nt) antibodies on this envelope component. ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-17-1-105
更新日期：1984-02-01 00:00:00
abstract：:Fifteen randomly selected nasopharyngeal (NP) swab specimens (culture-negative for influenza A virus) were spiked with influenza A virus and the nucleic acids were extracted and subjected to PCR amplification with Thermus aquaticus (Taq) and T. thermophilus (Tth) DNA polymerases. Products of the expected size, and giv...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-47-12-1131
更新日期：1998-12-01 00:00:00
abstract：:Five hundred clinical group A streptococcal (GAS) isolates were collected in Belgium during the period 1 Nov. 1993 to 31 Oct. 1994. Clinical and laboratory data were recorded and isolates were characterised. The presence of the genes encoding streptococcal pyrogenic exotoxin types A (speA), B (speB), C (speC), F (speF...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/0022-1317-49-5-467
更新日期：2000-05-01 00:00:00
abstract：:Introduction. Ocular microsporidiosis is a significant emerging infectious disease reported in immunocompromised patients and immunocompetent persons throughout the world.Aim. To identify the pathogens responsible for human keratitis, via corneal scrapings.Methodology. Thirty-three hospitalized patients with epithelia...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.001164
更新日期：2020-03-01 00:00:00
