Abstract:
:CaNdt80p, the Candida albicans homologue of the Saccharomyces cerevisiae transcription factor ScNdt80p, has been identified as a positive regulator of CDR1, which encodes an efflux pump involved in drug resistance in C. albicans. To investigate the involvement of the putative DNA-binding domain of CaNdt80p in drug resistance, chimeras of CaNdt80p and ScNdt80p were constructed. Interestingly, the DNA-binding domain of ScNdt80p could functionally complement that of CaNdt80p to activate CDR1p-lacZ in S. cerevisiae. Consistently, CaNdt80p containing a mutation in the DNA-binding domain failed to activate CDR1p-lacZ in S. cerevisiae. Furthermore, a copy of CaNDT80 with the same mutation also failed to complement the drug-sensitive phenotype caused by a null mutation in C. albicans. Thus, the DNA-binding domain of CaNdt80p is critical for its function in drug resistance in C. albicans.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Wang JS,Yang YL,Wu CJ,Ouyang KJ,Tseng KY,Chen CG,Wang H,Lo HJdoi
10.1099/jmm.0.46650-0subject
Has Abstractpub_date
2006-10-01 00:00:00pages
1403-1411issue
Pt 10eissn
0022-2615issn
1473-5644journal_volume
55pub_type
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