A mixed infection of Leuconostoc lactis and vancomycin-resistant Enterococcus in a liver transplant recipient.

Abstract:

:Bacterial infection in patients who have undergone liver transplantation is a major complication of the procedure. Leuconostoc spp. are important pathogenic bacteria in individuals with poor immune function, especially transplant patients. In this report, we describe the case of a 45-year-old Asian male liver transplant recipient who was initially preliminarily diagnosed with infection with Leuconostoc pseudomesenteroides by using the microbial tests of the VITEK 2 system and the aesculin hydrolysis test, and with vancomycin-resistant Enterococcus. Subsequently, the Leuconostoc isolate was identified as Leuconostoc lactis by 16S rRNA gene partial sequencing. In this paper, we discuss our identification of L. lactis based on physiological characteristics and molecular methodology. Accurate identification of these infections is important for the outcome; use of 16S rRNA gene sequence analysis offers a rapid and precise diagnostic approach. Administration of the drug linezolid may be useful for the treatment of both Leuconostoc spp. and vancomycin-resistant Enterococcus infections. We suggest that clinical analysts should use molecular methods in addition to biochemical tests in order to identify Leuconostoc at the species level more accurately.

journal_name

J Med Microbiol

authors

Deng Y,Zhang Z,Xie Y,Xiao Y,Kang M,Fan H

doi

10.1099/jmm.0.045161-0

subject

Has Abstract

pub_date

2012-11-01 00:00:00

pages

1621-1624

issue

Pt 11

eissn

0022-2615

issn

1473-5644

journal_volume

61

pub_type

杂志文章
  • Cross-reactivity between six Enterobacteriaceae complete lipopolysaccharide core chemotypes.

    abstract::To gain insight into the value of lipopolysaccharide (LPS) core determinants for cross-protective immunisation the serological relationships between six complete (LPS) core types from Enterobacteriaceae were investigated. Hyperimmune sera were raised in mice by repeated immunisation with heat-killed strains of Salmone...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-48-5-433

    authors: Nnalue NA,Khan GN,Mustafa N

    更新日期:1999-05-01 00:00:00

  • Recurrent candidaemia in a neonate with Hirschsprung's disease: fluconazole resistance and genetic relatedness of eight Candida tropicalis isolates.

    abstract::The incidence of candidaemia among immunocompromised patients in Malaysia is increasing at an alarming rate. Isolation of clinical strains that are resistant to fluconazole has also risen markedly. We report here the repeated isolation of Candida tropicalis from the blood of a neonatal patient with Hirschsprung's dise...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46045-0

    authors: Chong PP,Chieng DC,Low LY,Hafeez A,Shamsudin MN,Seow HF,Ng KP

    更新日期:2006-04-01 00:00:00

  • Differential binding of Shiga toxin 2 to human and murine neutrophils.

    abstract::Shiga toxins (Stx1 and Stx2) are responsible for initiating haemolytic uraemic syndrome, a serious extraintestinal complication caused by enterohaemorrhagic Escherichia coli O157 : H7 infection in humans. Shiga toxins are classical AB(5)-type exotoxins, consisting of a globotriaosylceramide (Gb(3))-binding B subunit p...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.47282-0

    authors: Griener TP,Mulvey GL,Marcato P,Armstrong GD

    更新日期:2007-11-01 00:00:00

  • Flow cytometric analysis of Clostridium difficile adherence to human intestinal epithelial cells.

    abstract::Clostridium difficile is the most common cause of diarrhoea in hospitalised patients. Bacterial adherence to gut epithelial cells is a likely prerequisite to infection and toxin production. A novel flow cytometric method was developed for detecting adherence of C. difficile to human colonic and small intestinal epithe...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-50-6-526

    authors: Drudy D,O'Donoghue DP,Baird A,Fenelon L,O'Farrelly C

    更新日期:2001-06-01 00:00:00

  • The bactericidal activity of DR-3355, an optically active isomer of ofloxacin.

    abstract::The bactericidal activity of compound DR-3355, an optically active isomer of ofloxacin, was measured against Escherichia coli, Staphylococcus aureus and S. epidermidis, in nutrient broth and in phosphate-buffered saline. DR-3355 was found to be approximately twice as active as ofloxacin in terms of the concentration a...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-30-3-227

    authors: Lewin CS,Amyes SG

    更新日期:1989-11-01 00:00:00

  • Variation in the persistence of Escherichia coli O157:H7 in experimentally inoculated 6-week-old conventional lambs.

    abstract::Six-week-old lambs were inoculated orally with 10(9) cfu of an antibiotic-resistance marked four-strain mixture of enterohaemorrhagic Escherichia coli (EHEC) O157:H7 to investigate faecal excretion and intestinal colonisation. In the first experiment, three E. coli O157:H7 isolates were not detected in the faeces of a...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-51-12-1032

    authors: Cookson AL,Wales AD,Roe JM,Hayes CM,Pearson GR,Woodward MJ

    更新日期:2002-12-01 00:00:00

  • Prevalence of childhood diarrhoea-associated Escherichia coli in Thailand.

    abstract::Escherichia coli isolates (n=2629) were collected between 1996 and 2000 from 2100 Thai children less than 12 years of age with acute diarrhoea. Enterotoxigenic (ETEC), enteroinvasive (EIEC), Shiga-toxin-producing (STEC), enteropathogenic (EPEC) and enteroaggregative (EAEC) E. coli were identified by their virulence ma...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.05413-0

    authors: Ratchtrachenchai OA,Subpasu S,Hayashi H,Ba-Thein W

    更新日期:2004-03-01 00:00:00

  • A 55 kDa hypothetical membrane protein is an iron-regulated virulence factor of Francisella tularensis subsp. novicida U112.

    abstract::Iron is an important nutritional requirement for bacteria due to its conserved role in many essential metabolic processes. As a consequence of the lack of freely available iron in the mammalian host, bacteria upregulate a range of virulence factors during infection. Transcriptional analysis of Francisella tularensis s...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.47190-0

    authors: Milne TS,Michell SL,Diaper H,Wikström P,Svensson K,Oyston PCF,Titball RW

    更新日期:2007-10-01 00:00:00

  • Hepatitis C virus genotyping in relation to neu-oncoprotein overexpression and the development of hepatocellular carcinoma.

    abstract::The distribution of hepatitis C virus (HCV) genotypes among Egyptian patients positive for anti-HCV was determined and their influence, when combined with neu-oncoprotein overexpression, on the development of hepatocellular carcinoma (HCC) was examined. The study groups included asymptomatic carriers (ASC) and patient...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-49-1-89

    authors: Zekri AN,Bahnassy AA,Shaarawy SM,Mansour OA,Maduar MA,Khaled HM,El-Ahmadi O

    更新日期:2000-01-01 00:00:00

  • Nucleotide sequence-based typing of meningococci directly from clinical samples.

    abstract::The unpredictable characteristics of meningococcal disease (MD) make outbreaks complicated to monitor and consequently lead to high levels of public anxiety. Traditional molecular techniques have been utilized in order to understand better the epidemiology of MD, but some have disadvantages such as being highly specia...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.05078-0

    authors: Diggle MA,Bell CM,Clarke SC

    更新日期:2003-06-01 00:00:00

  • Identification by PCR of Helicobacter pylori in subgingival plaque of adult periodontitis patients.

    abstract::The PCR was used to detect the presence of Helicobacter pylori in subgingival plaque samples from patients with adult periodontitis. Primers based upon the 16S ribosomal RNA (rRNA) gene sequence of H. pylori were used in a single round of PCR to amplify a 295-bp DNA fragment and the identity of the amplified products ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-48-3-317

    authors: Riggio MP,Lennon A

    更新日期:1999-03-01 00:00:00

  • Environmental contamination by Aspergillus spp. in laying hen farms and associated health risks for farm workers.

    abstract::Data on the occurrence and epidemiology of Aspergillus spp. in laying hens farms are scant. With the aims of determining levels of airborne contamination in laying hen farms and evaluating the potential risk of infection for workers and animals, 57 air samples from 19 sheds (Group I), 69 from faeces (Group II), 19 fro...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.065946-0

    authors: Cafarchia C,Camarda A,Iatta R,Danesi P,Favuzzi V,Di Paola G,Pugliese N,Caroli A,Montagna MT,Otranto D

    更新日期:2014-03-01 00:00:00

  • Rapid antibiotic sensitivity testing and trimethoprim-mediated filamentation of clinical isolates of the Enterobacteriaceae assayed on a novel porous culture support.

    abstract::A porous inorganic material (Anopore) was employed as a microbial culture and microcolony imaging support. Rapid Anopore-based antibiotic sensitivity testing (AST) methods were developed to assess the growth of clinical isolates, with the primary focus on testing the response of the Enterobacteriaceae to trimethoprim,...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46585-0

    authors: Ingham CJ,van den Ende M,Wever PC,Schneeberger PM

    更新日期:2006-11-01 00:00:00

  • Assessment of the in vitro and in vivo activity of atorvastatin against Candida albicans.

    abstract::Aim. The aim of this work was to characterize the response of Candida albicans to atorvastatin, and to assess its in vivo antifungal capability.Methodology. The effect of atorvastatin on the growth and viability of C. albicans was assessed. The ability of the statin to alter cell permeability was quantified by measuri...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001065

    authors: Ajdidi A,Sheehan G,Abu Elteen K,Kavanagh K

    更新日期:2019-10-01 00:00:00

  • The role of HSV-induced Fc- and C3b (i)-receptors in bacterial adherence.

    abstract::Herpes simplex virus type-1 (HSV-1) induces Fc- and C3b(i)-receptors on infected cells. The role of these receptors in bacterial superinfection was studied by comparing the adherence of non-opsonised and opsonised bacteria to HSV-infected and non-infected HEp-2 cells. A flow cytometric adherence assay, based on the fl...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-40-1-48

    authors: De Graaf-Miltenburg LA,Van Vliet KE,Ten Hagen TL,Verhoef J,Van Strijp JA

    更新日期:1994-01-01 00:00:00

  • The use of bacteriophages to differentiate serologically cross-reactive isolates of Klebsiella pneumoniae.

    abstract::In serological typing of Klebsiella pneumoniae strains from human, equine and environmental sources, the capsular identity of many isolates could not be determined because of serological cross-reactivity. A panel of 91 bacteriophages able to lyse each of the 77 capsular serotypes of K. pneumoniae was isolated and test...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-41-6-423

    authors: Pieroni P,Rennie RP,Ziola B,Deneer HG

    更新日期:1994-12-01 00:00:00

  • Cytotoxicity of Bacillus piliformis.

    abstract::Seven isolates of B. piliformis, the agent of Tyzzer's disease, obtained from various host species, were examined for cytotoxic activity by incubating culture filtrates on BRL 3A rat-hepatocyte and 3T3 mouse-fibroblast cell lines. One isolate exhibited cytopathic effects on BRL 3A cells, but not on 3T3 cells. Three ot...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-37-1-77

    authors: Riley LK,Caffrey CJ,Musille VS,Meyer JK

    更新日期:1992-07-01 00:00:00

  • Resistance of mice to genital infection with Neisseria gonorrhoeae.

    abstract::Five strains of mice (C3H, CBA, BALB/c, TO and ICR) were inoculated intra-vaginally with Neisseria gonorrhoeae in an attempt to produce an animal model of gonorrhoea. Of a total of 68 mice inoculated, only three (4.4%) were culture-positive after 3 days. Histological examination of both the genital mucosa of inoculate...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-30-1-33

    authors: Johnson AP,Tuffrey M,Taylor-Robinson D

    更新日期:1989-09-01 00:00:00

  • Virulent Toxoplasma gondii strain RH promotes T-cell-independent overproduction of proinflammatory cytokines IL12 and gamma-interferon.

    abstract::The aim of this study was the analysis of the cytokine response in BALB/c mice infected with the highly virulent RH or the weakly virulent Beverley strains of Toxoplasma gondii. Analysis of cytokine messages showed increased expression of IL12, IFN-gamma and TNF-alpha, but not IL4 mRNAs in spleen cells after infection...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.04860-0

    authors: Nguyen TD,Bigaignon G,Markine-Goriaynoff D,Heremans H,Nguyen TN,Warnier G,Delmee M,Warny M,Wolf SF,Uyttenhove C,Van Snick J,Coutelier JP

    更新日期:2003-10-01 00:00:00

  • Identification of urinary pathogens by the RAPIDEC ur system.

    abstract::A commercial method (RAPIDEC ur; bioMérieux UK Ltd, Basingstoke) and an in-house scheme based on conventional tests were compared for the identification of 179 commonly encountered gram-negative urinary pathogens. The methods identified correctly to genus level 97% and 84% of organisms, respectively. RAPIDEC ur was su...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-43-1-72

    authors: Batchelor BI

    更新日期:1995-07-01 00:00:00

  • Comparative genome and evolution analysis of the locus of enterocyte effacement from enteropathogenic Escherichia coli Deng and its transcriptional response to ciprofloxacin.

    abstract:PURPOSE:In this study, we aimed to investigate the genomic characteristics and evolution of pathogenicity islands of an enteropathogenic Escherichia coli (EPEC) strain, and to obtain a transcriptional profile of EPEC under different concentrations of ciprofloxacin using microarray analysis. METHODOLOGY:The complete EP...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000790

    authors: Chen Z,Cheng H,Pan W,Zheng J,Li D,Lin F,Yu Z,Deng Q

    更新日期:2018-09-01 00:00:00

  • The subcutaneous inoculation of pH 6 antigen mutants of Yersinia pestis does not affect virulence and immune response in mice.

    abstract::Two isogenic sets of Yersinia pestis strains were generated, composed of wild-type strains 231 and I-1996, their non-polar pH 6(-) mutants with deletions in the psaA gene that codes for its structural subunit or the whole operon, as well as strains with restored ability for temperature- and pH-dependent synthesis of a...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.005678-0

    authors: Anisimov AP,Bakhteeva IV,Panfertsev EA,Svetoch TE,Kravchenko TB,Platonov ME,Titareva GM,Kombarova TI,Ivanov SA,Rakin AV,Amoako KK,Dentovskaya SV

    更新日期:2009-01-01 00:00:00

  • Prevalence and characterization of Clostridium perfringens from the faecal microbiota of elderly Irish subjects.

    abstract::The aim of this study was to investigate the diversity and composition of the intestinal microbiota of elderly subjects using a combination of culture-dependent techniques and 16S rRNA gene amplicon sequencing. The study was performed as part of the ELDERMET project, in which 368 faecal samples were assessed for viabl...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.052258-0

    authors: Lakshminarayanan B,Harris HMB,Coakley M,O'Sullivan Ó,Stanton C,Pruteanu M,Shanahan F,O'Toole PW,Ross RP,On Behalf Of The Eldermet Consortium

    更新日期:2013-03-01 00:00:00

  • Epigallocatechin gallate suppresses expression of receptor activator of NF-kappaB ligand (RANKL) in Staphylococcus aureus infection in osteoblast-like NRG cells.

    abstract::Catechin, a constituent of tea, possesses various bioactivities. In particular, the most abundant catechin in tea is epigallocatechin gallate (EGCg), which has an anti-inflammatory effect. In the present study, the usability of EGCg for osteomyelitis treatment was examined. Osteomyelitis is a difficult disease to cure...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.47029-0

    authors: Ishida I,Kohda C,Yanagawa Y,Miyaoka H,Shimamura T

    更新日期:2007-08-01 00:00:00

  • Probiotics to minimize the disruption of faecal microbiota in healthy subjects undergoing antibiotic therapy.

    abstract::A novel combination of culturing and DNA-based terminal restriction fragment length polymorphism (TRFLP) analysis was used to investigate the effect of probiotics on antibiotic-induced gut microbiota alterations to determine if a probiotic preparation containing bifidobacteria and lactobacilli, taken during and after ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.47615-0

    authors: Engelbrektson A,Korzenik JR,Pittler A,Sanders ME,Klaenhammer TR,Leyer G,Kitts CL

    更新日期:2009-05-01 00:00:00

  • Cryptic plasmids in hospital isolates of Providencia stuarti.

    abstract::The distribution of cryptic plasmids among 123 isolates of Providencia stuarti from a hospital ward during a prospective epidemiological study is reported. Two closely related stable plasmids (34 Kb and 36 Kb) were identified by restriction endonuclease digest analysis of plasmid DNA. One or other of these cryptic pla...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-18-2-277

    authors: Hawkey PM,Bennett PM,Hawkey CA

    更新日期:1984-10-01 00:00:00

  • Klebsiella pneumoniae meningitis induces memory impairment and increases pro-inflammatory host response in the central nervous system of Wistar rats.

    abstract::Klebsiella pneumoniae meningitis has recently become an increasingly common cause of central nervous system infection. The invasion of bacteria within the subarachnoid space stimulates the release of pro-inflammatory cytokines and chemokines, triggering a host immune response. The aim of the present study was to evalu...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.063289-0

    authors: Barichello T,Simões LR,Valvassori SS,Generoso JS,Aveline PEDV,Dominguini D,Elias SG,Vilela MC,Quevedo J,Teixeira AL

    更新日期:2014-01-01 00:00:00

  • Detection of Clostridium difficile cytotoxin and Clostridium perfringens enterotoxin in cases of diarrhoea in the community.

    abstract::Faecal specimens from 843 cases of diarrhoea in the community were tested for the presence of Clostridium difficile cytotoxin and Clostridium perfringens enterotoxin. C. difficile cytotoxin was detected in faecal specimens from 0.6 % of cases aged at least 2 years by using a Vero cell assay. Factors associated with de...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.05119-0

    authors: Forward LJ,Tompkins DS,Brett MM

    更新日期:2003-09-01 00:00:00

  • In vitro activity of azithromycin, newer quinolones and cephalosporins in ciprofloxacin-resistant Salmonella causing enteric fever.

    abstract::The therapeutic alternatives available for use against ciprofloxacin-resistant enteric fever isolates in an endemic area are limited. The antibiotics currently available are the quinolones, third-generation cephalosporins and conventional first-line drugs. In this study, the MICs of various newer drugs were determined...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.47353-0

    authors: Capoor MR,Rawat D,Nair D,Hasan AS,Deb M,Aggarwal P,Pillai P

    更新日期:2007-11-01 00:00:00

  • Analysis of Epstein-Barr virus and cellular gene expression during the early phases of Epstein-Barr virus lytic induction.

    abstract::In order to develop novel host/pathogen real-time PCR assays for routine diagnostic use, early gene expression patterns from both Epstein-Barr virus (EBV) and Raji cells were examined after inducing the lytic life cycle using 12-O-tetradecanoyl-13-phorbol ester and sodium butyrate. Real-time PCR identified several hig...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000352

    authors: Auburn H,Zuckerman M,Smith M

    更新日期:2016-11-01 00:00:00