A simple blood-culture bacterial pellet preparation for faster accurate direct bacterial identification and antibiotic susceptibility testing with the VITEK 2 system.

abstract：:Erysipelothrix rhusiopathiae is a zoonotic pathogen that causes erysipelas in a variety of animals. In humans, in contrast to the cutaneous form called erysipeloid, which is an occupational disease and common in individuals who handle raw meat and fish, invasive systemic infections are unusual. E. rhusiopathiae expres...

journal_title：Journal of medical microbiology

authors： Taguchi Y,Shiraiwa K,Ogawa Y,Oi T,Nakamura S,Okumura J,Shimoji Y

更新日期：2020-10-01 00:00:00

abstract：:A colony hybridisation method with enzyme-labelled oligonucleotide probes was developed to detect the gene for toxic shock syndrome toxin 1 (tst). For rapid identification, bacterial colonies were transferred from agar plates directly on to nylon membranes. These procedures took only 3 h. Results obtained by this test...

journal_title：Journal of medical microbiology

authors： Shimaoka M,Yoh M,Takarada Y,Yamamoto K,Honda T

更新日期：1996-03-01 00:00:00

abstract：:Mice immunised by the oral or intraperitoneal route with a live aromatic-dependent strain of Salmonella typhimurium exhibited significantly less protection against oral challenge with 50 LD50 of an ovine isolate of S. typhimurium (12313) than when a bovine isolate with the same O antigens and phage-type as strain 1231...

journal_title：Journal of medical microbiology

authors： Mukkur TK,McDowell GH,Stocker BA,Lascelles AK

更新日期：1987-08-01 00:00:00

abstract：:The potential of exocellular carbohydrate antigens of Staphylococcus epidermidis as markers of infection in bone was investigated by immunoblotting and enzyme-linked immunosorbent assay (ELISA). Exocellular antigens were prepared by gel filtration chromatography of concentrated brain heart infusion culture supernates....

journal_title：Journal of medical microbiology

authors： Lambert PA,Van Maurik A,Parvatham S,Akhtar Z,Fraise AP,Krikler SJ

更新日期：1996-05-01 00:00:00

abstract：:Catechin, a constituent of tea, possesses various bioactivities. In particular, the most abundant catechin in tea is epigallocatechin gallate (EGCg), which has an anti-inflammatory effect. In the present study, the usability of EGCg for osteomyelitis treatment was examined. Osteomyelitis is a difficult disease to cure...

journal_title：Journal of medical microbiology

authors： Ishida I,Kohda C,Yanagawa Y,Miyaoka H,Shimamura T

更新日期：2007-08-01 00:00:00

abstract：:An evaluation of five ELISA methods for the diagnosis of acute toxoplasmosis was undertaken by comparing a laboratory-produced ELISA employing two recombinant Toxoplasma gondii polypeptides as antigen with a laboratory-produced ELISA and three commercially available ELISAs employing traditional parasite antigen prepar...

journal_title：Journal of medical microbiology

authors： Johnson AM,Roberts H,Tenter AM

更新日期：1992-12-01 00:00:00

abstract：:Burkholderia pseudomallei is isolated frequently from the soil in regions where the disease melioidosis occurs. However, recent surveys in Thailand have shown that the frequency of isolation of the organism from soil samples is not directly related to the incidence of melioidosis in an area. To determine whether strai...

journal_title：Journal of medical microbiology

authors： Trakulsomboon S,Dance DA,Smith MD,White NJ,Pitt TL

更新日期：1997-07-01 00:00:00

abstract：:Pandoraea species have emerged as opportunistic pathogens among cystic fibrosis (CF) and non-CF patients. Pandoraea pulmonicola is the predominant Pandoraea species among Irish CF patients. The objective of this study was to investigate the pathogenicity and potential mechanisms of virulence of Irish P. pulmonicola is...

journal_title：Journal of medical microbiology

authors： Costello A,Herbert G,Fabunmi L,Schaffer K,Kavanagh KA,Caraher EM,Callaghan M,McClean S

更新日期：2011-03-01 00:00:00

abstract：PURPOSE:Antimicrobial resistance (AMR) is of increasing global concern, threatening to undermine recent progress in reducing child and neonatal mortality. Repurposing older antimicrobials is a prominent strategy to combat multidrug-resistant sepsis. A potential agent is fosfomycin, however, there is scarce data regardi...

journal_title：Journal of medical microbiology

authors： Williams PCM,Waichungo J,Gordon NC,Sharland M,Murunga S,Kamau A,Berkley JA

更新日期：2019-05-01 00:00:00

abstract：:Nearly 1000 sera from children were tested by immunoelectro-osmophoresis against BK virus, and age-specific prevalence rates were estimated from birth until the age of 12 years. Declining rates during the first 12 months showed the waning of passive immunity, which at birth reflects the mother's immune status. The cha...

journal_title：Journal of medical microbiology

authors： Dei R,Marmo F,Corte D,Sampietro MG,Franceschini E,Urbano P

更新日期：1982-08-01 00:00:00

abstract：PURPOSE:We aimed to evaluate a PCR-based technique for the diagnosis of mucormycosis and the identification of fungi from fresh tissue specimens in patients with rhino-orbito-cerebral-mucormycosis (ROCM). METHODOLOGY:Fifty cases of ROCM were included in the study. Conventional identification was performed using micros...

journal_title：Journal of medical microbiology

authors： Zaman K,Rudramurthy SM,Das A,Panda N,Honnavar P,Kaur H,Chakrabarti A

更新日期：2017-08-01 00:00:00

abstract：:The prevalence of Shiga toxin-producing Escherichia coli (STEC) and its characteristics were determined among hospitalized patients with diarrhoea and children with diarrhoea in an urban slum community of Dhaka city using sensitive culture and PCR methods. Stool samples were collected from 410 patients with diarrhoea ...

journal_title：Journal of medical microbiology

authors： Islam MA,Heuvelink AE,de Boer E,Sturm PD,Beumer RR,Zwietering MH,Faruque ASG,Haque R,Sack DA,Talukder KA

更新日期：2007-03-01 00:00:00

abstract：:In this study, we investigated the in vitro antifungal effects of itraconazole/voriconazole (ITR/VRC) alone and in combination with tetrandrine (TET) against 23 clinical isolates of A. fumigatus using a chequerboard microdilution method. The dynamic antifungal effects of TET with ITR/VRC against A. fumigatus were asse...

journal_title：Journal of medical microbiology

authors： Li SX,Song YJ,Zhang LL,Shi JP,Ma ZL,Guo H,Dong HY,Li YM,Zhang H

更新日期：2015-09-01 00:00:00

abstract：:The attachment of Helicobacter pylori to the human gastric mucosa is a complex process involving several specific structures recognised by the cell surface receptors. Sialylated multivalent high mol. wt glycoproteins have been shown to inhibit H. pylori sialic acid-specific haemagglutination. This study explored wheth...

journal_title：Journal of medical microbiology

authors： Wang X,Hirmo S,Willén R,Wadström T

更新日期：2001-05-01 00:00:00

abstract：:Enteroaggregative Escherichia coli (EAEC) is an important agent of diarrhoeal diseases worldwide. The role of EAEC virulence factors in the clinical outcome of infection is not completely defined. This case-control study investigated the prevalence of EAEC, its virulence genes and the antimicrobial resistance profile ...

journal_title：Journal of medical microbiology

authors： Bafandeh S,Haghi F,Zeighami H

更新日期：2015-05-01 00:00:00

abstract：:We studied in vitro ceftaroline combinations against 61 meticillin-resistant Staphylococcus aureus isolates; 18 of them were also resistant to linezolid, using overlapping E-test method. Daptomycin-ceftaroline combination obtained lower fractional inhibitory concentration values, in comparison with those including van...

journal_title：Journal of medical microbiology

authors： García AB,Candel FJ,López L,Chiarella F,Viñuela-Prieto JM

更新日期：2016-10-01 00:00:00

abstract：:Small round viruses (SRV) were isolated from the faeces of diarrhoeic calves from three farms. All three SRV preparations caused diarrhoea experimentally in gnotobiotic calves. Each preparation contained viral particles of two morphological types, "astrovirus-like" and "calicivirus-like", and from one preparation the ...

journal_title：Journal of medical microbiology

authors： Woode GN,Bridger JC

更新日期：1978-11-01 00:00:00

abstract：:Introduction. Ocular microsporidiosis is a significant emerging infectious disease reported in immunocompromised patients and immunocompetent persons throughout the world.Aim. To identify the pathogens responsible for human keratitis, via corneal scrapings.Methodology. Thirty-three hospitalized patients with epithelia...

journal_title：Journal of medical microbiology

authors： Sanpool O,Thanathanee O,Laummuanwai P,Maleewong W,Intapan PM

更新日期：2020-03-01 00:00:00

abstract：:The growth of 29 strains of Acinetobacter spp. on semi-solid media was studied; 19 showed surface swarming and 14 produced channels ("ditches") in the agar that do not seem to have been described previously. An attempt was made to define the cultural and physical conditions for the demonstration of these phenomena. Po...

journal_title：Journal of medical microbiology

authors： Barker J,Maxted H

更新日期：1975-08-01 00:00:00

abstract：:Intravenous injection of eight human strains of Campylobacter fetus ss fetus and Campylobacter jejuni into mice at various stages of pregnancy demonstrated significant strain differences in ability to affect implantation of the fertilised ovum and to cause resorption of the mouse fetus. Implantation was significantly ...

journal_title：Journal of medical microbiology

authors： O'Sullivan AM,Doré CJ,Coid CR

更新日期：1988-01-01 00:00:00

abstract：:The frequency distribution of plasmids in a representative collection of Escherichia coli and other enterobacteria was compared with the frequencies predicted by the Poisson distribution. The distribution of E. coli plasmids did not differ significantly (p greater than 0.2) whereas the difference between observed and ...

journal_title：Journal of medical microbiology

authors： Platt DJ

更新日期：1987-05-01 00:00:00

abstract：:The susceptibility of a strain of Giardia lamblia to benzimidazole carbamates, 5-nitroimidazoles, nitrofurans and other drugs was studied in vitro. Albendazole was the most active compound, with a 50% inhibitory concentration (IC50) of 0.01 mg/L and a minimal lethal concentration (MLC) of less than 0.04 mg/L; the IC50...

journal_title：Journal of medical microbiology

authors： Cedillo-Rivera R,Muñoz O

更新日期：1992-09-01 00:00:00

abstract：:In a prospective study between February 2003 and June 2004, stool specimens of children less than 2 years of age with diarrhoea (n=218) and without diarrhoea (n=86), living in Vitória, Espírito Santo, Brazil, were examined for the presence of diarrhoeagenic Escherichia coli. E. coli isolates were tested by colony blot...

journal_title：Journal of medical microbiology

authors： Spano LC,Sadovsky ADI,Segui PN,Saick KW,Kitagawa SMS,Pereira FEL,Fagundes-Neto U,Scaletsky ICA

更新日期：2008-03-01 00:00:00

abstract：:Three strains of Plesiomonas shigelloides isolated from patients with diarrhoea were agglutinated with Shigella flexneri 6 antiserum in slide and tube tests. All the strains were also agglutinated with a monoclonal antibody to the common group 1 antigen shared between S. flexneri serotypes and S. dysenteriae type 1. F...

journal_title：Journal of medical microbiology

authors： Albert MJ,Ansaruzzaman M,Qadri F,Hossain A,Kibriya AK,Haider K,Nahar S,Faruque SM,Alam AN

更新日期：1993-09-01 00:00:00

abstract：PURPOSE:The aim of this work was to evaluate and optimize the identification of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica (usually known as the classical Bordetella species) using Bruker Biotyper matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF M...

journal_title：Journal of medical microbiology

authors： Zintgraff J,Irazu L,Lara CS,Rodriguez M,Santos M

更新日期：2018-12-01 00:00:00

abstract：:Different aspects of the terms strain, clone and species are discussed. The term strain is commonly used to denote a pure culture - here called 'the strain in the taxonomic sense' - but does also refer to a natural concept closely related to the clone. The term clone on the other hand is used both in a general and in ...

journal_title：Journal of medical microbiology

authors： Dijkshoorn L,Ursing BM,Ursing JB

更新日期：2000-05-01 00:00:00

abstract：:The incidence of candidaemia among immunocompromised patients in Malaysia is increasing at an alarming rate. Isolation of clinical strains that are resistant to fluconazole has also risen markedly. We report here the repeated isolation of Candida tropicalis from the blood of a neonatal patient with Hirschsprung's dise...

journal_title：Journal of medical microbiology

authors： Chong PP,Chieng DC,Low LY,Hafeez A,Shamsudin MN,Seow HF,Ng KP

更新日期：2006-04-01 00:00:00

abstract：:Micro-organisms isolated from the oral cavity may translocate to the lower airways during mechanical ventilation (MV) leading to ventilator-associated pneumonia (VAP). Changes within the dental plaque microbiome during MV have been documented previously, primarily using culture-based techniques. The aim of this study ...

journal_title：Journal of medical microbiology

authors： Sands KM,Twigg JA,Lewis MAO,Wise MP,Marchesi JR,Smith A,Wilson MJ,Williams DW

更新日期：2016-02-01 00:00:00

abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) is recognised as an important cause of nosocomial infection. The spread of some MRSA epidemic clones is well documented. In Brazil, and more recently in Portugal, a considerable number of hospital infections has been caused by a unique multiresistant MRSA clone design...

journal_title：Journal of medical microbiology

authors： Coimbra MVDS,Teixeira LA,Ramos RLB,Predari SC,Castello L,Famiglietti A,Vay C,Klan L,Figueiredo AMS

更新日期：2000-02-01 00:00:00

abstract：:One hundred and eight strains of Aeromonas from clinical and environmental samples were speciated. Seven species were identified, the most prevalent of which was A. hydrophila. Experimental studies in an animal model with 36 representative strains of different species revealed that all strains could cause significant ...

journal_title：Journal of medical microbiology

authors： Chaudhury A,Nath G,Shukla BN,Sanyal SC

更新日期：1996-06-01 00:00:00