Abstract:
:Two monoclonal antibodies (MAbs) to a 36-kDa extracellular metalloprotease (PSCP) from Burkholderia (Pseudomonas) cepacia were found to react with thermolysin, Pseudomonas aeruginosa elastase, alkaline protease (Apr) and LasA, Serratia marcescens protease (SMP), Aeromonas hydrophila protease (AhP), and both the lethal factor (LF) and protective antigen (PA) of Bacillus anthracis on immunoblots. The MAbs were capable of neutralising the proteolytic activity of thermolysin, P. aeruginosa elastase and PSCP but not that of Apr, SMP, and AhP. These results suggest that these MAbs may be able to differentiate between the thermolysin and serralysin family of metalloproteases on the basis of their neutralisation capability and could, therefore, be useful tools in the characterisation of new bacterial proteases.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Kooi C,Sokol PAdoi
10.1099/00222615-45-3-219subject
Has Abstractpub_date
1996-09-01 00:00:00pages
219-25issue
3eissn
0022-2615issn
1473-5644journal_volume
45pub_type
杂志文章abstract::The inhibitory effect of human immunodeficiency virus (HIV) proteinase inhibitors amprenavir and saquinavir and antifungal agents terbinafine, ketoconazole, amphotericin B and ciclopiroxolamine on aspartyl proteinases (Saps) secreted by Candida albicans was tested in an in vitro spectophotometric assay. As expected, b...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.05048-0
更新日期:2003-03-01 00:00:00
abstract::Streptolysin O (SLO) is a potent cytolytic toxin produced by almost all strains of group A streptococci and is considered an important virulence factor for this organism. In this study we investigated the effect of allicin and aqueous garlic extracts on the haemolytic activity of SLO. All tested materials potentially ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.019539-0
更新日期:2010-09-01 00:00:00
abstract::The objective of this study was to investigate the antimicrobial susceptibility of the organisms isolated from the nasopharynx of children who presented with acute otitis media (AOM) or otitis media that recurred after amoxicillin therapy. Nasopharyngeal cultures obtained from 72 patients, 40 with AOM and 32 with recu...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.45819-0
更新日期:2005-01-01 00:00:00
abstract:PURPOSE:Membrane fluidity to a large extent is governed by the presence of branched-chain fatty acids (BCFAs). Branched-chain α-keto acid dehydrogenase (BKD) is the key enzyme in BCFA synthesis. A Staphylococcus aureus BKD-deficient strain still produced substantial levels of BCFAs. Pyruvate dehydrogenase (PDH) with st...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000707
更新日期:2018-04-01 00:00:00
abstract::Nearly 1000 sera from children were tested by immunoelectro-osmophoresis against BK virus, and age-specific prevalence rates were estimated from birth until the age of 12 years. Declining rates during the first 12 months showed the waning of passive immunity, which at birth reflects the mother's immune status. The cha...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-15-3-285
更新日期:1982-08-01 00:00:00
abstract:PURPOSE:This study aimed to detect and identify staphylococcal enterotoxin (SE) genes in methicillin-resistant Staphylococcus pseudintermedius (MRSP) strains from different sources, and to investigate the relationship between their sequence types (STs) and SE gene patterns. METHODOLOGY:The profiles of 17 SE genes in 9...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000748
更新日期:2018-06-01 00:00:00
abstract::Strains of Staphylococcus aureus resistant to gentamicin and methicillin first appeared in Dublin hospitals in 1976, and rapidly became widely disseminated. The number of patients infected or colonised increased throughout the period of study, especially in 1979 and 1980. Most isolates were from burns, surgical wounds...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-16-2-117
更新日期:1983-05-01 00:00:00
abstract::An evaluation of five ELISA methods for the diagnosis of acute toxoplasmosis was undertaken by comparing a laboratory-produced ELISA employing two recombinant Toxoplasma gondii polypeptides as antigen with a laboratory-produced ELISA and three commercially available ELISAs employing traditional parasite antigen prepar...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-37-6-404
更新日期:1992-12-01 00:00:00
abstract::Clostridium difficile is the most common cause of antibiotic-associated diarrhoea. Antibiotics are presumed to disturb the normal intestinal microbiota, leading to depletion of the barrier effect and colonization by pathogenic bacteria. This first step of infection includes adherence to epithelial cells. We investigat...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.47676-0
更新日期:2008-06-01 00:00:00
abstract:PURPOSE:Infections associated with Acinetobacter baumannii represent an increasing threat in healthcare settings. Therefore, we investigated the epidemiological relationship between clinical isolates of A. baumannii obtained from patients in a university hospital in Bandar Abbas in southern Iran. METHODOLOGY:Sixty-fou...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000619
更新日期:2017-11-01 00:00:00
abstract::The locus responsible for mucoid colony morphology in five independent clinical isolates of Pseudomonas aeruginosa from cystic fibrosis patients have been transferred by means of pM060-mediated conjugation to the genetically characterised strain P. aeruginosa PAO. Genetic mapping has shown that in all five strains the...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-21-4-331
更新日期:1986-06-01 00:00:00
abstract::Sixty-three Staphylococcus aureus strains (40 from clinical sources and 23 from food sources) were examined for toxic shock syndrome toxin-1 (TSST-1) using PCR, phage typed using the international phage set (IPS) and tested for their susceptibility to antibiotics. Only three strains (all from clinical sources) were po...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46162-0
更新日期:2006-02-01 00:00:00
abstract::The therapeutic alternatives available for use against ciprofloxacin-resistant enteric fever isolates in an endemic area are limited. The antibiotics currently available are the quinolones, third-generation cephalosporins and conventional first-line drugs. In this study, the MICs of various newer drugs were determined...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.47353-0
更新日期:2007-11-01 00:00:00
abstract::There is a paucity of information about the factors associated with oral colonization with Candida species and the changes associated with antiretroviral (ARV) therapy. This study investigated the role of ARV therapy and other factors in a study population. Relevant clinical and laboratory information was obtained and...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.047522-0
更新日期:2013-01-01 00:00:00
abstract::Cholera, caused by Vibrio cholerae, results in significant morbidity and mortality worldwide, including Thailand. Representative V. cholerae strains associated with endemic cholera (n = 32), including strains (n = 3) from surface water sources, in Khon Kaen, Thailand (2003-2011), were subjected to microbiological, mol...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.053801-0
更新日期:2013-04-01 00:00:00
abstract::Herein we describe a real-time quantitative PCR assay for evaluating the adherence of Clostridium difficile to differentiated human intestinal Caco-2 cells. Our investigations demonstrated that the method, employing the C. difficile-specific triose-phosphate isomerase gene, is as reliable but less time-consuming than ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.019752-0
更新日期:2010-08-01 00:00:00
abstract::Acute hypercytokinaemia represents an imbalance of pro-inflammatory and anti-inflammatory cytokines, and is believed to be responsible for the development of acute respiratory distress syndrome and multiple organ failure in severe cases of avian (H5N1) influenza. Although neuraminidase inhibitors are effective in trea...
journal_title:Journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.1099/jmm.0.47124-0
更新日期:2007-07-01 00:00:00
abstract::The relative pathogenicity of Candida krusei and C. albicans was investigated by assessing their colonisation and infectivity of the Sprague-Dawley rat oral mucosa. During an initial 21-week period with intermittent oral inoculation, both Candida spp. demonstrated variable surface colonisation of the oral mucosa. Afte...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-47-12-1047
更新日期:1998-12-01 00:00:00
abstract::Experiments were performed to determine whether a modern flow cytometer could be used to study bacterial populations in suspension, with particular reference to their morphological characteristics and their responses to antibiotics. The FACScan, a commercial benchtop flow cytometer fitted with an air-cooled laser, des...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-39-2-147
更新日期:1993-08-01 00:00:00
abstract:PURPOSE:This paper aims to evaluate the antimicrobial resistance of Esherichia coli isolates from children under 5 years old, with and without diarrhoea, who were hospital outpatients in Culiacan, Sinaloa, Mexico. It also looks at the antimicrobial activity of fruit extracts against selected multidrug-resistant (MDR) E...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000548
更新日期:2017-07-01 00:00:00
abstract::Three strains of Plesiomonas shigelloides isolated from patients with diarrhoea were agglutinated with Shigella flexneri 6 antiserum in slide and tube tests. All the strains were also agglutinated with a monoclonal antibody to the common group 1 antigen shared between S. flexneri serotypes and S. dysenteriae type 1. F...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-39-3-211
更新日期:1993-09-01 00:00:00
abstract::Resistance to gentamicin, tobramycin and kanamycin (GmrTmrKmr) in strains of Staphylococcus aureus isolated from clinical sources in Australia is mediated by a 4.7 kb transposable element, designated Tn4001. A 2.5 kb HindIII fragment which maps symmetrically within Tn4001, and encompasses the aminoglycoside-resistance...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-23-2-101
更新日期:1987-03-01 00:00:00
abstract:PURPOSE:Non-host-adapted Salmonella serovars, including the common human food-borne pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium), are opportunistic pathogens that can colonize food-producing animals without causing overt disease. Interventions against Salmonella are needed to enhance food safety, p...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000482
更新日期:2017-05-01 00:00:00
abstract::Stenotrophomonas maltophilia KH has two acid beta-lactamases with isoelectric points (pIs) of 4.6 and 5.4, and several basic beta-lactamases (pIs >7.0) that produce a ladder-shaped pattern by IEF. An isogenic L2 mutant, KHL2(xylE), was constructed by gene replacement. From IEF and native PAGE zymograms of strains KH a...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000380-0
更新日期:2009-03-01 00:00:00
abstract::Staphylococcus aureus is a leading aetiologic agent of nosocomial- and community-acquired infectious diseases worldwide. The public health concern regarding staphylococcal infections is inflated by the increasing occurrence of multidrug-resistant strains, e.g. multidrug- and meticillin-resistant S.aureus (MDR MRSA). T...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000343
更新日期:2016-10-01 00:00:00
abstract::Historically, enteropathogenic Escherichia coli (EPEC) are a well-known cause of outbreaks of infantile diarrhoea associated with morbidity and mortality in England. The aim of this study was to provide an update on the microbiology and epidemiology of strains of EPEC in England between 2010 and 2012. A wide range of ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.062380-0
更新日期:2013-10-01 00:00:00
abstract::The determinant responsible for the ability of Bacteroides spp. to inhibit polymorph phagocytic killing of aerobic organisms has not yet been identified. Therefore, the roles of lipopolysaccharide and capsular polysaccharide of B. fragilis were investigated. Serum-resistant and serum-sensitive strains of Proteus mirab...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-17-3-259
更新日期:1984-06-01 00:00:00
abstract::Pulsed-field gel electrophoresis typing was performed on a retrospective set of 129 Moraxella catarrhalis isolates obtained over a 20 month period from 70 children admitted to, or presenting at, the Erasmus University Medical Center, Rotterdam, The Netherlands. The mean age of the children (at the end of the study) wa...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.05251-0
更新日期:2003-09-01 00:00:00
abstract:PURPOSE:Persister cells, a subpopulation of tolerant cells within the bacterial culture, are commonly thought to be responsible for antibiotic therapy failure and infection recurrence. Klebsiella pneumoniae is a notorious human pathogen for its increasing resistance to antibiotics and wide involvement in severe infecti...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000680
更新日期:2018-03-01 00:00:00
abstract::Bifidobacteria are dominant in the gut of full-term infants, although colonisation by them is often delayed in preterm neonates. Bifidobacteria are recognised to have beneficial effects on digestive disorders and they might prevent neonatal necrotising enterocolitis (NEC), a gastrointestinal disease that predominantly...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-48-1-89
更新日期:1999-01-01 00:00:00