Differentiation of thermolysins and serralysins by monoclonal antibodies.

Abstract:

:Two monoclonal antibodies (MAbs) to a 36-kDa extracellular metalloprotease (PSCP) from Burkholderia (Pseudomonas) cepacia were found to react with thermolysin, Pseudomonas aeruginosa elastase, alkaline protease (Apr) and LasA, Serratia marcescens protease (SMP), Aeromonas hydrophila protease (AhP), and both the lethal factor (LF) and protective antigen (PA) of Bacillus anthracis on immunoblots. The MAbs were capable of neutralising the proteolytic activity of thermolysin, P. aeruginosa elastase and PSCP but not that of Apr, SMP, and AhP. These results suggest that these MAbs may be able to differentiate between the thermolysin and serralysin family of metalloproteases on the basis of their neutralisation capability and could, therefore, be useful tools in the characterisation of new bacterial proteases.

journal_name

J Med Microbiol

authors

Kooi C,Sokol PA

doi

10.1099/00222615-45-3-219

subject

Has Abstract

pub_date

1996-09-01 00:00:00

pages

219-25

issue

3

eissn

0022-2615

issn

1473-5644

journal_volume

45

pub_type

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