Abstract:
:A total of 1878 non-duplicate clinical Escherichia coli isolates (comprising 1711 urinary isolates and 167 blood-culture isolates), which were collected from multiple centres in Hong Kong during 1996-2008, were used to investigate the prevalence and molecular epidemiology of plasmid-mediated fosfomycin (fos) resistance genes. Eighteen of the 1878 clinical E. coli isolates were fosfomycin resistant, of which six were fosA3 positive and two were positive for another fosA variant (designated fosKP96). No isolates had the fosC2 gene. The clones of the eight isolates were diverse: sequence type (ST) 95 (n = 2), ST118 (n = 1), ST131 (n = 1), ST617 (n = 1), ST648 (n = 1), ST1488 (n = 1) and ST2847 (n = 1). In the isolates, fosA3 and blaCTX-M genes were co-harboured on conjugative plasmids with F2:A-:B- (n = 2), N (n = 1), F-:A-:B1 and N (n = 1) and untypable (n = 2) replicons. Both fosKP96-carrying plasmids belonged to replicon N. RFLP analysis showed that the two F2:A-:B- plasmids carrying fosA3 and blaCTX-M-3 genes shared the same pattern. Complete sequencing of one of the two F2:A-:B- plasmids, pFOS-HK151325 (69 768 bp) demonstrated it to be >99 % identical to the previously sequenced plasmid pHK23a originating from a pig E. coli isolate in the same region. This study demonstrated the dissemination of fosA3 genes in diverse E. coli clones on multiple blaCTX-M-carrying plasmid types, of which F2:A-:B- plasmids closely related to pHK23a were shared by isolates from human and animal sources.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Ho PL,Chan J,Lo WU,Lai EL,Cheung YY,Lau TCK,Chow KHdoi
10.1099/jmm.0.062653-0subject
Has Abstractpub_date
2013-11-01 00:00:00pages
1707-1713issue
Pt 11eissn
0022-2615issn
1473-5644journal_volume
62pub_type
杂志文章abstract::In a survey of 3302 people in an area of central Sweden, tinea versicolor was diagnosed in 0.5% of males and 0.3% of females; all those infected were adults. Tinea versicolor is much less common in Sweden than in the tropics. ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-16-4-501
更新日期:1983-11-01 00:00:00
abstract::The rapid identification of pathogenic yeasts is a crucial step in ensuring that effective antifungal treatment is started as early as possible. CandiSelect 4 (CS4; Bio-Rad) is a new chromogenic medium for the isolation of fungi, the direct identification of Candida albicans and the presumptive identification of the m...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46715-0
更新日期:2007-04-01 00:00:00
abstract::Diabetic mice were significantly more susceptible than normal mice to bladder infection with Enterobacter cloacae or Serratia marcescens but not to intraperitoneal infection. Normal urine inhibited the growth of E. cloacae and S. marcescens, whereas urine from diabetic mice permitted multiplication. The addition of ur...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-30-2-105
更新日期:1989-10-01 00:00:00
abstract::Detection of latent Mycobacterium tuberculosis is a challenge in the diagnosis of asymptomatic, subclinical tuberculosis. We report the development of an immunofluorescence technique to visualize and enumerate M. tuberculosis in latently infected rabbit lungs where no acid-fast-stained organisms were seen and no culti...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.081091-0
更新日期:2014-11-01 00:00:00
abstract::Active efflux has an important role in the antimicrobial resistance of Campylobacter jejuni and Campylobacter coli. The effects of two putative efflux pump inhibitors (EPIs), phenylalanine-arginine β-naphthylamide and 1-(1-naphthylmethyl)-piperazine, and the effects of inactivation of the cmeB,cmeF and cmeR genes on r...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.041467-0
更新日期:2012-06-01 00:00:00
abstract::Bifidobacteria are dominant in the gut of full-term infants, although colonisation by them is often delayed in preterm neonates. Bifidobacteria are recognised to have beneficial effects on digestive disorders and they might prevent neonatal necrotising enterocolitis (NEC), a gastrointestinal disease that predominantly...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-48-1-89
更新日期:1999-01-01 00:00:00
abstract::The effect of CO2 on the growth of 31 strains of Candida albicans was studied in serum and in a defined medium containing urea, ammonium chloride, asparagine, glutamine or acetamide as the nitrogen source. CO2 10% enhanced the mycelial growth of all strains when the medium contained an appropriate constituent to media...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-22-3-203
更新日期:1986-11-01 00:00:00
abstract::Radiometric selective inhibition tests were developed and evaluated for the rapid differentiation of Mycobacterium spp. Both a p-nitrobenzoic acid (PNB) test and a commercially-prepared p-nitro-alpha-acetylamino-beta-hydroxypropiophenone (NAP) test successfully differentiated M. tuberculosis and M. bovis from "atypica...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-30-3-175
更新日期:1989-11-01 00:00:00
abstract::Intestinal absorption of arbutin (p-hydroxyphenyl-beta-glucoside), a non-metabolised analogue of d-glucose, and net flux of water out of the small intestinal lumen were found to be decreased in vitro in rats fed with Candida albicans. In rats on a protein-deficient diet and fed C. albicans there was net secretion of w...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-13-1-103
更新日期:1980-02-01 00:00:00
abstract::Clostridium difficile is a frequent cause of severe, recurrent, post-antibiotic diarrhoea and pseudomembranous colitis. Its pathogenicity is mediated mainly by two toxins, TcdA and TcdB. However, different adhesins have also been described as important colonization factors which are implicated in the first step of the...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.029553-0
更新日期:2011-08-01 00:00:00
abstract::Two chlamydial enzyme immunoassays, Chlamydiazyme and IDEIA, were evaluated in a mouse model of chlamydial genital-tract infection. The Chlamydiazyme assay and the IDEIA were assessed on specimens from 10 and 11 mice, respectively. The animals were infected with Chlamydia trachomatis, strain SA2f, and the results obta...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-24-2-169
更新日期:1987-09-01 00:00:00
abstract::This study analysed the invasiveness of Listeria monocytogenes into enterocyte-like Caco-2 cells in which iron depletion was achieved by picolinic acid treatment. Both entry and intracellular multiplication varied depending on the endogenous iron content of bacterial and eukaryotic cells. The behaviour within enterocy...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/0022-1317-49-8-681
更新日期:2000-08-01 00:00:00
abstract::Although Neisseria gonorrhoeae can use haem as the sole exogenous iron source for growth in vitro, the mechanism of haem-iron uptake in the gonococcus is unknown. Two haemin-binding proteins (HmBPs) of 97 and 44 Kda were isolated by batch ligand affinity-chromatography from whole cells or total membranes of gonococci ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-36-2-121
更新日期:1992-02-01 00:00:00
abstract::The identity and motility of enterococci isolated as urinary pathogens were compared with those of strains from other sources. The majority of isolates from all sources proved to be Streptococcus faecalis of S. faecium but eight of 117 urinary strains and seven of 127 non-urinary strains belonged to a group intermedia...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-15-1-127
更新日期:1982-02-01 00:00:00
abstract::To gain insight into the value of lipopolysaccharide (LPS) core determinants for cross-protective immunisation the serological relationships between six complete (LPS) core types from Enterobacteriaceae were investigated. Hyperimmune sera were raised in mice by repeated immunisation with heat-killed strains of Salmone...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-48-5-433
更新日期:1999-05-01 00:00:00
abstract::Forty carbapenem-resistant Klebsiella pneumoniae isolates were recovered from 28 patients from various sites in an intensive care unit in Zhejiang Provincial People's Hospital, China, over a 6 month period. PFGE analysis indicated that the 40 strains were all closely related. The MICs of carbapenems varied from 16 to ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.015826-0
更新日期:2011-07-01 00:00:00
abstract::Pseudomonas aeruginosa is an opportunistic pathogen that poses a threat in clinical settings due to its intrinsic and acquired resistance to a wide spectrum of antibiotics. Additionally, the presence of a subpopulation of cells surviving high concentrations of antibiotics, called persisters, makes it virtually impossi...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.019703-0
更新日期:2011-03-01 00:00:00
abstract::A provisional serotyping scheme for Bacillus cereus has been developed. Eighteen selected cultures of B. cereus isolated from foods and clinical specimens were used to prepare agglutinating sera against the flagellar antigen. There were no significant cross-reactions between the sera. The sera were used to type 137 is...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-8-4-543
更新日期:1975-11-01 00:00:00
abstract::Tests with 140 strains representing Escherichia coli, Klebsiella aerogenes, K. ozaenae, Proteus mirabilis, P. vulgaris, P. rettgeri and P. morganii in a defined medium supplemented with 0-09M dimethylamine (DMA) and 0-1M potassium nitrate showed that at least 89% of the 136 strains able to reduce nitrates produced up ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-9-2-211
更新日期:1976-05-01 00:00:00
abstract::Resistance to desiccation and to skin fatty acids was measured in three groups of methicillin-resistant Staphylococcus aureus (MRSA) strains and a group of control strains. Organisms from a large outbreak on a special care baby unit (SCBU), where MRSA had been isolated from staff hands but not from the environment, we...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-36-1-56
更新日期:1992-01-01 00:00:00
abstract::Campylobacter fetus causes severe infections in humans and can be isolated from various mammals and reptiles. However, although poultry are considered to be the main reservoir of Campylobacter jejuni, little is known about the presence of C. fetus in poultry. Thus, specific pathogen-free chickens were experimentally i...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46628-0
更新日期:2006-09-01 00:00:00
abstract::The activities of three sulphonamides and trimethoprim against strains of Pseudomonas aeruginosa have been studied. Sulphadiazine had most activity, sulphadimidine had little, and the activity of sulphamethoxazole was intermediate. According to their sensitivity to sulphamethoxazole, strains were divided into two grou...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-10-3-273
更新日期:1977-08-01 00:00:00
abstract::This study investigated the molecular mechanisms of carbapenem resistance in clinical isolates of Klebsiella pneumoniae from Korea and the clinical outcomes of resistant K. pneumoniae infection. Sixteen K. pneumoniae isolates showing resistance to carbapenems collected from a tertiary-care hospital were examined for t...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.037036-0
更新日期:2012-02-01 00:00:00
abstract::Early reports of Candida krusei in man describe the organism as a transient, infrequent isolate of minor clinical significance inhabiting the mucosal surfaces. More recently it has emerged as a notable pathogen with a spectrum of clinical manifestations such as fungaemia, endophthalmitis, arthritis and endocarditis, m...
journal_title:Journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.1099/00222615-41-5-295
更新日期:1994-11-01 00:00:00
abstract::The eubacterial population was studied in faecal samples of related and unrelated children. Temporal temperature gradient gel electrophoresis (TTGE) provided a snapshot of the bacterial population and allowed calculation of the degree of similarity in the predominant faecal microflora of identical twin pairs, fraterna...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46189-0
更新日期:2005-12-01 00:00:00
abstract::Lysostaphin is a glycylglycine endopeptidase. It cleaves the pentaglycine cross-bridge structure unique to the staphylococcal cell wall and is considered to be a potential drug for Staphylococcus aureus. In the present study, the in vitro activity of recombinant lysostaphin was investigated in 257 S. aureus isolates c...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46788-0
更新日期:2007-01-01 00:00:00
abstract::A collection of 201 isolates of Staphylococcus aureus was examined: 152 methicillin-sensitive S. aureus (MSSA) comprised 48 blood culture isolates (BC) and 58 isolates from routine diagnostic specimens (RD) from Glasgow Royal Infirmary (GRI), and 46 strains from nasal swabs of patients attending a general practitioner...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-36-3-164
更新日期:1992-03-01 00:00:00
abstract::Catechin, a constituent of tea, possesses various bioactivities. In particular, the most abundant catechin in tea is epigallocatechin gallate (EGCg), which has an anti-inflammatory effect. In the present study, the usability of EGCg for osteomyelitis treatment was examined. Osteomyelitis is a difficult disease to cure...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.47029-0
更新日期:2007-08-01 00:00:00
abstract::Klebsiella pneumoniae meningitis has recently become an increasingly common cause of central nervous system infection. The invasion of bacteria within the subarachnoid space stimulates the release of pro-inflammatory cytokines and chemokines, triggering a host immune response. The aim of the present study was to evalu...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.063289-0
更新日期:2014-01-01 00:00:00
abstract::A collection of 820 isolates of streptococci and aerococci from human blood or internal organs was classified by means of a set of cultural and biochemical tests. Of these, 719 (712 streptococci and 7 aerococci) were from patients believed to be suffering from a systemic infection: endocarditis 317; a purulent lesion ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-9-3-275
更新日期:1976-08-01 00:00:00