Radiometric studies on the use of selective inhibitors in the identification of Mycobacterium spp.

abstract：:Experiments were performed to determine whether a modern flow cytometer could be used to study bacterial populations in suspension, with particular reference to their morphological characteristics and their responses to antibiotics. The FACScan, a commercial benchtop flow cytometer fitted with an air-cooled laser, des...

journal_title：Journal of medical microbiology

authors： Gant VA,Warnes G,Phillips I,Savidge GF

更新日期：1993-08-01 00:00:00

abstract：:Enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) are members of the species Human enterovirus A, and are both major and independent aetiological agents of hand-foot-and-mouth disease. The human enterovirus (HEV) 5' untranslated region (UTR) is fundamentally important for efficient virus replication and for virulen...

journal_title：Journal of medical microbiology

authors： Zhou F,Kong F,Wang B,McPhie K,Gilbert GL,Dwyer DE

更新日期：2011-03-01 00:00:00

abstract：:The growth inhibiting activity of transferrins, citrate, 2-2' dipyridyl and desferrioxamine methanesulphonate towards Legionella spp. and their serogroups was investigated. The inhibitory activity of all these compounds depended upon the iron-free state of the molecules and was abolished by saturation with iron. No ba...

journal_title：Journal of medical microbiology

authors： Goldoni P,Visca P,Pastoris MC,Valenti P,Orsi N

更新日期：1991-02-01 00:00:00

abstract：:Since 2007, there has been a re-emergence of cholera outbreaks in northern Vietnam. To understand the molecular epidemiological relatedness and determine the antibiotic susceptibility profiles of responsible V. cholerae O1 outbreak strains, a representative collection of 100 V. cholerae O1 strains was characterized. V...

journal_title：Journal of medical microbiology

authors： Tran HD,Alam M,Trung NV,Van Kinh N,Nguyen HH,Pham VC,Ansaruzzaman M,Rashed SM,Bhuiyan NA,Dao TT,Endtz HP,Wertheim HFL

更新日期：2012-03-01 00:00:00

abstract：PURPOSE:Extensively drug-resistant (XDR) strains of Acinetobacter baumannii are being reported worldwide, and they are associated with high morbidity and mortality rates. These strains are considered to be the highest priority for the development of new antibacterial agents. Therefore, we aimed to develop an effective ...

journal_title：Journal of medical microbiology

authors： Wintachai P,Naknaen A,Pomwised R,Voravuthikunchai SP,Smith DR

更新日期：2019-07-01 00:00:00

abstract：:A study of 1314 patients with malignancies was made to determine the prevalence of Serratia marcescens in surveillance, diagnostic, and environmental cultures. Sera obtained from a commercial source were used to determine serotypic distributions of the S. marcescens strains isolated during a 51-month period. S. marces...

journal_title：Journal of medical microbiology

authors： Young VM,Moody MR,Morris MJ

更新日期：1980-05-01 00:00:00

abstract：:Epidemic methicillin-resistant Staphylococcus aureus types 15 and 16 (EMRSA-15 and EMRSA-16) are the dominant types of MRSA found in UK hospitals, but accurate designation of strains has been difficult. Restriction fragment length polymorphism (RFLP) profiles of seven core virulence genes were used to classify unambig...

journal_title：Journal of medical microbiology

authors： Moore PCL,Lindsay JA

更新日期：2002-06-01 00:00:00

abstract：:The purpose of this study was to compare the frequency of recovery of aerobic and anaerobic organisms with interfering capability against group A beta-haemolytic streptococci (GABHS) and beta-lactamase-producing bacteria (BLPB) from the tonsils of GABHS carriers and non-carriers. The presence of aerobic and anaerobic ...

journal_title：Journal of medical microbiology

authors： Brook I,Gober AE

更新日期：2006-12-01 00:00:00

abstract：:Eight monoclonal antibodies (MAbs) were produced from mice immunised with whole cells of heat-killed Pasteurella multocida type A which had been cultured under iron-restricted conditions. The MAbs were selected by an enzyme-linked immunosorbent assay (ELISA) in which the antigen consisted of whole bacteria of the immu...

journal_title：Journal of medical microbiology

authors： Wijewardana TG,Wilson CF,Gilmour NJ,Poxton IR

更新日期：1990-12-01 00:00:00

abstract：:Small numbers of bacteria capable of growing on agar supplemented with amoxycillin 40 mg/L were isolated from the saliva of 9 out of 20 adult volunteers in a previous study. All the bacteria were identified as Streptococcus sanguis although no strains produced dextran in conventional tests. However, using a specific a...

journal_title：Journal of medical microbiology

authors： Marsh PD,West AA,Keevil CW

更新日期：1987-11-01 00:00:00

abstract：:Fifteen randomly selected nasopharyngeal (NP) swab specimens (culture-negative for influenza A virus) were spiked with influenza A virus and the nucleic acids were extracted and subjected to PCR amplification with Thermus aquaticus (Taq) and T. thermophilus (Tth) DNA polymerases. Products of the expected size, and giv...

journal_title：Journal of medical microbiology

authors： Poddar SK,Sawyer MH,Connor JD

更新日期：1998-12-01 00:00:00

abstract：PURPOSE:Non-albicansCandida species have emerged as fungal pathogens that cause invasive infections, with many of these species displaying resistance to commonly used antifungal agents. This study was confined to studying the characteristics of clinical isolates of the C. rugosa complex and C. pararugosa species. METH...

journal_title：Journal of medical microbiology

authors： Peremalo T,Madhavan P,Hamzah S,Than L,Wong EH,Nasir MDM,Chong PP,Ng KP

更新日期：2019-03-01 00:00:00

abstract：:The rapid identification of pathogenic yeasts is a crucial step in ensuring that effective antifungal treatment is started as early as possible. CandiSelect 4 (CS4; Bio-Rad) is a new chromogenic medium for the isolation of fungi, the direct identification of Candida albicans and the presumptive identification of the m...

journal_title：Journal of medical microbiology

authors： Sendid B,François N,Standaert A,Dehecq E,Zerimech F,Camus D,Poulain D

更新日期：2007-04-01 00:00:00

abstract：:Prior to the advent of the Haemophilus influenzae type b vaccine, invasive infections due to H. influenzae type f were rarely described. However, the epidemiology of H. influenzae is changing. While the incidence of invasive infections due to H. influenzae is declining in children, such infections are becoming more co...

journal_title：Journal of medical microbiology

authors： Suarez CJ,Glover WA,Cowan J,Smith A,Clarridge JE

更新日期：2013-04-01 00:00:00

abstract：:Interaction of human polymorphonuclear leucocytes (PMNL) and influenza virus (IFV) was studied in vitro. At 0 degree C, the viral particles were bound extensively to the surface of the PMNL membrane with a ratio of about 1000 virus particles to a single PMNL. The binding was sensitive to neuraminidase, suggesting atta...

journal_title：Journal of medical microbiology

authors： Yamamoto K,Suzuki K,Suzuki K,Mizuno S

更新日期：1989-03-01 00:00:00

abstract：:Amplification and sequence analysis of the 16S rRNA gene can be applied to detect and identify bacteria in clinical samples. We examined 75 clinical samples (17 culture-positive, 58 culture-negative) prospectively by two different PCR protocols, amplifying either a single fragment (1343 bp) or two fragments (762/598 b...

journal_title：Journal of medical microbiology

authors： Jenkins C,Ling CL,Ciesielczuk HL,Lockwood J,Hopkins S,McHugh TD,Gillespie SH,Kibbler CC

更新日期：2012-04-01 00:00:00

abstract：PURPOSE:With an increase in the numbers of bacterial isolates resistant to first-line antibiotics, there has been a revival in the use of older drugs including fosfomycin with novel mechanisms of action. We aimed to investigate the prevalence and genotypic nature of fosfomycin resistance in Escherichia coli from urinar...

journal_title：Journal of medical microbiology

authors： Cottell JL,Webber MA

更新日期：2019-02-01 00:00:00

abstract：:The aim of this study was to establish bacterial profiles in gastric biopsy specimens from patients with Helicobacter pylori-associated gastritis by means of temporal temperature gradient gel electrophoresis (TTGE) of PCR-amplified 16S rDNA fragments. Specimens from eight patients with asymptomatic gastritis and five ...

journal_title：Journal of medical microbiology

authors： Monstein HJ,Tiveljung A,Kraft CH,Borch K,Jonasson J

更新日期：2000-09-01 00:00:00

abstract：:The intrathecal synthesis of IgM and IgG antibodies to Borrelia burgdorferi sonicate, to recombinant flagellin (41 kDa) and to a tryptic peptide of the flagellin (14-kDa fragment) was determined by ELISA in paired cerebrospinal fluid (CSF) and serum samples from 35 patients with Lyme neuroborreliosis (LNB) and in 10 p...

journal_title：Journal of medical microbiology

authors： Kaiser R,Rasiah C,Gassmann G,Vogt A,Lücking CH

更新日期：1993-10-01 00:00:00

abstract：:Pyrolysis mass spectrometry (PyMS), a highly discriminatory method for comparison of isolates, was used to assess the homogeneity of colonies taken from apparently pure cultures of Escherichia coli in 10 urine specimens. Ten randomly selected colonies were subcultured from each urine for comparison. For six urines, th...

journal_title：Journal of medical microbiology

authors： Freeman R,Sisson PR,Burdess D

更新日期：1996-11-01 00:00:00

abstract：:The aim of this study was to improve the identification of Mycobacterium species in the context of a UK teaching hospital. Real-time PCR assays were established to enable the rapid differentiation between Mycobacterium tuberculosis (MTB) complex and Mycobacterium species other than tuberculosis (MOTT), followed by 16S...

journal_title：Journal of medical microbiology

authors： Williams KJ,Ling CL,Jenkins C,Gillespie SH,McHugh TD

更新日期：2007-05-01 00:00:00

abstract：:A commercial method (RAPIDEC ur; bioMérieux UK Ltd, Basingstoke) and an in-house scheme based on conventional tests were compared for the identification of 179 commonly encountered gram-negative urinary pathogens. The methods identified correctly to genus level 97% and 84% of organisms, respectively. RAPIDEC ur was su...

journal_title：Journal of medical microbiology

authors： Batchelor BI

更新日期：1995-07-01 00:00:00

abstract：:We report a case of septicaemia with prolonged, refractory hypotension related to Moraxella osloensis isolated in a non-immunocompromised paediatric patient. ...

journal_title：Journal of medical microbiology

authors： Dien Bard J,Lewinski M,Summanen PH,Deville JG

更新日期：2011-01-01 00:00:00

abstract：:Production of a meningococcal vaccine capable of generating long-lasting immunity in all age groups is still a high priority worldwide. Iron-regulated outer-membrane proteins have attracted considerable attention in recent years and it has become increasingly evident that the meningococcal transferrin-binding proteins...

journal_title：Journal of medical microbiology

authors： Ala'Aldeen DA

更新日期：1996-04-01 00:00:00

abstract：:Alpha protoxin of Staphylococcus aureus "Wood 46" was activated by trypsin which had been coupled to carboxymethylcellulose, as indicated by the toxin's ability to hydrolyse tosyl-arginine methylester (TAME). A Lineweaver-Burk plot of the degradation of TAME by toxin and trypsin showed that toxin had a greater affinit...

journal_title：Journal of medical microbiology

authors： Wiseman GM,Caird JD,Fackrell HB

更新日期：1975-02-01 00:00:00

abstract：:To study the influence of normal associated microbiota on systemic immunological responses during experimental Chagas' disease, germ-free and conventional NIH Swiss mice were infected with Y strain of Trypanosoma cruzi. Although no statistical differences in mortality and parasitaemia were found, conventional mice sho...

journal_title：Journal of medical microbiology

authors： Duarte R,Silva AM,Vieira LQ,Afonso LCC,Nicoli JR

更新日期：2004-08-01 00:00:00

abstract：:During 2003, Vibrio cholerae O1 Ogawa was the predominant serotype among diarrhoeal patients admitted to different hospitals in India. With the exception of 3 strains from Kolkata, none of 172 strains examined exhibited resistance to tetracycline, but 45.7 % showed reduced susceptibility to ciprofloxacin. Extensive mo...

journal_title：Journal of medical microbiology

authors： Chatterjee S,Ghosh K,Raychoudhuri A,Pan A,Bhattacharya MK,Mukhopadhyay AK,Ramamurthy T,Bhattacharya SK,Nandy RK

更新日期：2007-06-01 00:00:00

abstract：:A porous inorganic material (Anopore) was employed as a microbial culture and microcolony imaging support. Rapid Anopore-based antibiotic sensitivity testing (AST) methods were developed to assess the growth of clinical isolates, with the primary focus on testing the response of the Enterobacteriaceae to trimethoprim,...

journal_title：Journal of medical microbiology

authors： Ingham CJ,van den Ende M,Wever PC,Schneeberger PM

更新日期：2006-11-01 00:00:00

abstract：PURPOSE:To analyse the role of virulence factors (VFs) and host in Klebsiella pneumoniae upper urinary tract infections (UTIs) in renal transplant (RTx) recipients. METHODOLOGY:Clinical and demographic data were registered prospectively. Phylogenetic background of K. pneumoniae isolates was analysed by PCR melting pro...

journal_title：Journal of medical microbiology

authors： Gołębiewska JE,Krawczyk B,Wysocka M,Ewiak A,Komarnicka J,Bronk M,Rutkowski B,Dębska-Ślizień A

更新日期：2019-03-01 00:00:00

abstract：:The global emergence of antibiotic-resistant strains of Pseudomonas aeruginosa poses a major threat in both hospital environments and the community. P. aeruginosa is an opportunistic human pathogen, and it also infects a wide range of model organisms including the nematode Caenorhabditis elegans. Quorum sensing (QS) m...

journal_title：Journal of medical microbiology

authors： Ganesh PS,Rai RV

更新日期：2016-12-01 00:00:00