Development and application of two independent real-time PCR assays to detect clinically relevant Mucorales species.

abstract：:Leifsonia aquatica is an aquatic coryneform rod that is capable of forming biofilms in environmental water sources. It has rarely been associated with human infections and its pathogenicity and clinical significance are uncertain. We describe a case of catheter-related bloodstream infection in a haemodialysis patient....

journal_title：Journal of medical microbiology

authors： Porte L,Soto A,Andrighetti D,Dabanch J,Braun S,Saldivia A,Flores JC,Wozniak A,García P,Weitzel T

更新日期：2012-06-01 00:00:00

abstract：:Herein we describe a real-time quantitative PCR assay for evaluating the adherence of Clostridium difficile to differentiated human intestinal Caco-2 cells. Our investigations demonstrated that the method, employing the C. difficile-specific triose-phosphate isomerase gene, is as reliable but less time-consuming than ...

journal_title：Journal of medical microbiology

authors： Dingle T,Mulvey GL,Humphries RM,Armstrong GD

更新日期：2010-08-01 00:00:00

abstract：:Yeast identification and in vitro susceptibility testing provide helpful information for appropriate administration of antifungal treatments; however, few reports from the Latin American region have been published. The aim of this study was to identify the species present in isolates from bloodstream infections diagno...

journal_title：Journal of medical microbiology

authors： Bustamante B,Martins MA,Bonfietti LX,Szeszs MW,Jacobs J,Garcia C,Melhem MSC

更新日期：2014-06-01 00:00:00

abstract：:Hepatitis E virus (HEV) is an RNA virus that is an important cause of both acute and chronic hepatitis worldwide. To date, there are eight HEV genotypes that can infect mammals. HEV-1 and HEV-2 infect exclusively humans, while HEV-3 and HEV-4 infect humans and various animals, mainly pigs and deer. Additionally, two n...

journal_title：Journal of medical microbiology

authors： Al-Sadeq DW,Majdalawieh AF,Mesleh AG,Abdalla OM,Nasrallah GK

更新日期：2018-04-01 00:00:00

abstract：:The aim of this work was to evaluate the effects of beneficial human vaginal lactobacilli (Lb) on urogenital pathogens through in vitro and in vivo experiments. Co-aggregative and antimicrobial properties between five vaginal Lb strains and urogenital pathogens or potential pathogens (Streptococcus agalactiae, Staphyl...

journal_title：Journal of medical microbiology

authors： De Gregorio PR,Tomás MSJ,Terraf MCL,Nader-Macías MEF

更新日期：2014-05-01 00:00:00

abstract：:Escherichia coli (2), Vibrio cholerae (2) and Aeromonas sobria (1) strains were examined for their ability to grow and produce toxins in samples of ileostomy fluid. Three categories of response were observed: no detectable growth, growth without detectable toxin, and growth with detectable toxin. Clear differences wer...

journal_title：Journal of medical microbiology

authors： Ala Aldeen DA,Barer MR

更新日期：1989-04-01 00:00:00

abstract：:Cross-reactivity in a delayed-type hypersensitivity (DH) response was studied in mice immunised with live Salmonella typhi, S. paratyphi A and S. paratyphi B. Extensive cross-reactions outside the serogroup limits were observed. The ability of DH cross-reacting and non-cross-reacting sonicates to generate activated ma...

journal_title：Journal of medical microbiology

authors： Tiwari H,Kamat RS

更新日期：1986-05-01 00:00:00

abstract：:One hundred and twenty consecutive isolates of Neisseria gonorrhoeae from the County of Avon, UK, were examined by auxotyping and penicillin susceptibility testing. Requirements for proline (Pro), arginine (Arg), hypoxanthine (Hyp), uracil (Ura) and methionine (Met) were determined with a modified Heckels' medium. Pro...

journal_title：Journal of medical microbiology

authors： Copley CG,Egglestone SI

更新日期：1983-08-01 00:00:00

abstract：:Coccidioidal pericarditis is a condition found in approximately 1-5% of patients infected by Coccidioides species. It is associated with widely diverse clinical symptoms. This paper reports a case of coccidioidal pericarditis diagnosed by an in-house Coccidioides posadasii antigen and confirmed with mycological and mo...

journal_title：Journal of medical microbiology

authors： Brilhante RSN,Cordeiro RA,Rocha MFG,Fechine MAB,Furtado FM,Nagao-Dias AT,Camargo ZP,Sidrim JJC

更新日期：2008-10-01 00:00:00

abstract：:To gain a better understanding of the pathological role of interferon-gamma (IFN-gamma) in specific granuloma formation, IFN-gamma gene-deficient mice (BALB/c and C57BL/6) were produced. The IFN-gamma gene in embryonic stem (ES) cells was disrupted by inserting the beta-galactosidase gene (lacZ) and the neomycin resis...

journal_title：Journal of medical microbiology

authors： Sugawara I,Yamada H,Kazumi Y,Doi N,Otomo K,Aoki T,Mizuno S,Udagawa T,Tagawa Y,Iwakura Y

更新日期：1998-10-01 00:00:00

abstract：:The potential of exocellular carbohydrate antigens of Staphylococcus epidermidis as markers of infection in bone was investigated by immunoblotting and enzyme-linked immunosorbent assay (ELISA). Exocellular antigens were prepared by gel filtration chromatography of concentrated brain heart infusion culture supernates....

journal_title：Journal of medical microbiology

authors： Lambert PA,Van Maurik A,Parvatham S,Akhtar Z,Fraise AP,Krikler SJ

更新日期：1996-05-01 00:00:00

abstract：:Effects of four mucopolysaccharides and dextran sulphate on penicillin-induced lysis of Staphylococcus aureus FDA 209P were studied. Heparin and dextran sulphate inhibited lysis, whereas hyaluronic acid enhanced it. Chondroitin sulphates A and C had no effect. Incubation of S. aureus suspended in 0.03 M phosphate buff...

journal_title：Journal of medical microbiology

authors： Kiriyama T,Miyake Y,Sugai M,Kobayashi K,Yoshiga K,Takada K,Suginaka H

更新日期：1987-12-01 00:00:00

abstract：:The effect of pretreatment of denture acrylic with chlorhexidine gluconate on the subsequent adherence of Candida albicans GDH 2346 was measured in vitro. Adherence was significantly reduced by pretreatment with chlorhexidine; maximal inhibition was achieved by incubation at room temperature for 30 min in 2% chlorhexi...

journal_title：Journal of medical microbiology

authors： McCourtie J,MacFarlane TW,Samaranayake LP

更新日期：1985-08-01 00:00:00

abstract：:Human enterovirus 68 (HEV-68) is an enterovirus associated with respiratory illness. In China, no information about HEV-68 is available for children yet. This study aimed to investigate the presence of HEV-68 in mainland China between 2009 and 2012 and to explore the migration events of HEV-68 across the world. Among ...

journal_title：Journal of medical microbiology

authors： Lu QB,Wo Y,Wang HY,Wei MT,Zhang L,Yang H,Liu EM,Li TY,Zhao ZT,Liu W,Cao WC

更新日期：2014-03-01 00:00:00

abstract：PURPOSE:To determine the predominant strains of Bordetella pertussis in Greece during 2010-2015. METHODOLOGY:Infants and children (n=1150) (15 days to 14 years) of Greek, Roma and immigrant origin with different vaccination statuses were hospitalized in Athens, Greece with suspected pertussis infection. IS481/IS1001 r...

journal_title：Journal of medical microbiology

authors： Petridou E,Jensen CB,Arvanitidis A,Giannaki-Psinaki M,Michos A,Krogfelt KA,Petersen RF

更新日期：2018-03-01 00:00:00

abstract：:Bovine lactoferricin, a pepsin-generated antimicrobial peptide from bovine lactoferrin active against a wide range of bacteria, was tested for its ability to influence the adhesion and invasion of Yersinia enterocolitica and Yersinia pseudotuberculosis in HEp-2 cells. The addition of non-cytotoxic and non-bactericidal...

journal_title：Journal of medical microbiology

authors： Di Biase AM,Tinari A,Pietrantoni A,Antonini G,Valenti P,Conte MP,Superti F

更新日期：2004-05-01 00:00:00

abstract：:Purpose. Acute bacterial meningitis continues to be a potentially life threatening condition. Hospital-acquired meningitis is rapidly increasing and adding an immense burden to the health system due to the emergence of multidrug resistance isolates. The purpose of this study is to find the antibiotic susceptibility pa...

journal_title：Journal of medical microbiology

authors： Raza MS,Das BK,Goyal V,Lodha R,Chaudhry R,Sood S,Gautam H,Sreenivas V,Nair D,Mohapatra S,Kapil A

更新日期：2019-11-01 00:00:00

abstract：:Trichoderma longibrachiatum is an emerging pathogen in immunocompromised patients. We report a case of Trichoderma post-operative mediastinitis and peritonitis in a child with complex congenital cardiac disease and functional asplenia. The patient was treated unsuccessfully, initially with caspofungin alone followed b...

journal_title：Journal of medical microbiology

authors： Santillan Salas CF,Joshi AY,Dhiman N,Banerjee R,Huskins WC,Wengenack NL,Henry NK

更新日期：2011-12-01 00:00:00

abstract：:A commercial method (RAPIDEC ur; bioMérieux UK Ltd, Basingstoke) and an in-house scheme based on conventional tests were compared for the identification of 179 commonly encountered gram-negative urinary pathogens. The methods identified correctly to genus level 97% and 84% of organisms, respectively. RAPIDEC ur was su...

journal_title：Journal of medical microbiology

authors： Batchelor BI

更新日期：1995-07-01 00:00:00

abstract：:Members of the Streptococcus anginosus group (SAG) are frequently involved in pyogenic infections in humans. In the present study, the antimicrobial susceptibility of 141 clinical SAG isolates to six antimicrobial agents was analysed by agar dilution. All isolates were susceptible to penicillin, cefotaxime and vancomy...

journal_title：Journal of medical microbiology

authors： Asmah N,Eberspächer B,Regnath T,Arvand M

更新日期：2009-02-01 00:00:00

abstract：:Terminal restriction fragment-length polymorphism (T-RFLP) analysis was applied to characterize oral bacterial flora in saliva from 18 healthy subjects and 18 patients with periodontitis. The 16S rRNA genes (rDNAs) of oral bacteria and spirochaetes in saliva were amplified by PCR with a 6'carboxy-fluorescein (6-FAM)-l...

journal_title：Journal of medical microbiology

authors： Sakamoto M,Takeuchi Y,Umeda M,Ishikawa I,Benno Y

更新日期：2003-01-01 00:00:00

abstract：:During the first quarter of 1996, a major outbreak of clinical infection caused by multiresistant Klebsiella pneumoniae (MRKP) occurred in the neonatal ward of the 'Maternité Wassila Bourguiba' in Tunis, Tunisia. In total, 32 isolates of MRKP, comprising 23 clinical isolates and nine surveillance isolates, were recove...

journal_title：Journal of medical microbiology

authors： Ben-Hamouda T,Foulon T,Ben-Cheikh-Masmoudi A,Fendri C,Belhadj O,Ben-Mahrez K

更新日期：2003-05-01 00:00:00

abstract：:In January 2015, Public Health England and the United Kingdom (UK) Ministry of Defence investigated cases of diarrhoea and fever in military personnel recently returned to the UK after supporting the response to the Ebola epidemic in Sierra Leone. Tests for Ebola virus infection were negative. PCR tests detected the i...

journal_title：Journal of medical microbiology

authors： Todkill D,Pudney R,Terrell A,Tuck J,Hutley E,Dallman TJ,Jenkins C,Manuel R,Wade M,Puleston R,Hawker J

更新日期：2018-11-01 00:00:00

abstract：:Sixty-one isolates of Candida albicans were tested for their phospholipase activity and this parameter was correlated with pathogenicity in albino mice. Of the isolates tested, 57 (93%) showed appreciable phospholipase activity and of these, 55 (90%) were pathogenic to mice. A significant correlation was found between...

journal_title：Journal of medical microbiology

authors： Kothavade RJ,Panthaki MH

更新日期：1998-02-01 00:00:00

abstract：:There have been several outbreaks of botulism among poultry and wild birds in Sweden in recent years. The National Veterinary Institute of Sweden (SVA) has identified botulinum neurotoxin (BoNT)/C1 or the mosaic BoNT/C1D using the mouse bioassay. This is believed to be the first report on the application of the Endope...

journal_title：Journal of medical microbiology

authors： Hedeland M,Moura H,Båverud V,Woolfitt AR,Bondesson U,Barr JR

更新日期：2011-09-01 00:00:00

abstract：:Eighty-one bronchoalveolar lavage (BAL) specimens obtained from 26 HIV-infected, 45 non-HIV immunosuppressed and 10 immunocompetent patients with primary pulmonary diseases were analysed for the presence of Pneumocystis carinii by staining and by P. carinii 5S rDNA determined by PCR. P. carinii was observed by stainin...

journal_title：Journal of medical microbiology

authors： Takahashi T,Goto M,Endo T,Nakamura T,Yusa N,Sato N,Iwamoto A

更新日期：2002-07-01 00:00:00

abstract：:The susceptibility of a strain of Giardia lamblia to benzimidazole carbamates, 5-nitroimidazoles, nitrofurans and other drugs was studied in vitro. Albendazole was the most active compound, with a 50% inhibitory concentration (IC50) of 0.01 mg/L and a minimal lethal concentration (MLC) of less than 0.04 mg/L; the IC50...

journal_title：Journal of medical microbiology

authors： Cedillo-Rivera R,Muñoz O

更新日期：1992-09-01 00:00:00

abstract：:The in vitro activity of voriconazole was compared with those of fluconazole and itraconazole against 270 clinical isolates of yeasts from the mouths of patients receiving palliative care for advanced cancer. A broth micro-dilution assay as described by the National Committee for Clinical Laboratory Standards was empl...

journal_title：Journal of medical microbiology

authors： Bagg J,Sweeney MP,Davies AN,Jackson MS,Brailsford S

更新日期：2005-10-01 00:00:00

abstract：:A serial multiplex PCR approach was reformulated for pneumococcal serotyping to test 153 clinical isolates from children in Mozambique. This approach identified serotypes in 139 (90.8%) of 153 isolates; 126 (82.4%) were identified within two reactions. This approach in developing countries would require minimal traini...

journal_title：Journal of medical microbiology

authors： Morais L,Carvalho MDG,Roca A,Flannery B,Mandomando I,Soriano-Gabarró M,Sigauque B,Alonso P,Beall B

更新日期：2007-09-01 00:00:00

abstract：:Yersinia pseudotuberculosis was examined for its haemagglutinating activity and adherence to cultured epithelial cells (HEp-2) in relation to possession of a virulence (VW) plasmid and to growth conditions. VW-lacking (VW-) bacteria were isolated from ten VW+ strains of each serovar which, after they were grown on CFA...

journal_title：Journal of medical microbiology

authors： Kawaoka Y,Mitani T,Hoshina K,Otsuki K,Tsubokura M

更新日期：1988-03-01 00:00:00