Abstract：
:The distribution of hepatitis C virus (HCV) genotypes among Egyptian patients positive for anti-HCV was determined and their influence, when combined with neu-oncoprotein overexpression, on the development of hepatocellular carcinoma (HCC) was examined. The study groups included asymptomatic carriers (ASC) and patients with chronic active hepatitis (CAH) and HCC. HCV genomes were detected in the sera of 27 ASC, 29 CAH and 33 HCC patients known to have HCV infection defined by EIA and recombinant immunoblotting techniques (Inno-LiA) as well as by reverse transcriptase (RT)-PCR. The HCV genotype was determined by a reverse hybridisation technique (Inno-LiPA I and II), whereas neu-overexpression was detected by the Oncogene Science EIA Kit. Eighty-nine patients were eligible for HCV genotyping; 75 patients (84.3%) were infected with a single genotype, including 1a in 11 patients (12.4%), 1b in 2 patients (2.2%) and 2a in 10 patients (11.2%). Genotype 4 (a or c+d) was detected in 51 patients (57.3%) and only one patient had genotype 10a (1.2%). Fourteen patients (15.7%) showed mixed infection; eight of them had 1a+4 (a or c+d) and four had 2a+4 (a or c+d); the remaining two cases had 1a+2a and 1b+2a. The results revealed an increased incidence of genotype 4 in CAH and HCC patients in comparison with ASC. There was also a significant overexpression of neu-oncoprotein in CAH and HCC patients compared with ASC, which was significantly associated with subtype 4 infection. The results suggest that infection with subtype 1a and 4 HCV may be considered a risk factor for the induction of neu-overexpression and subsequent development of HCC.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Zekri AN,Bahnassy AA,Shaarawy SM,Mansour OA,Maduar MA,Khaled HM,El-Ahmadi Odoi
10.1099/0022-1317-49-1-89keywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
89-95issue
1eissn
0022-2615issn
1473-5644journal_volume
49pub_type
杂志文章abstract：:The ability of Streptococcus mutans to produce mutacins, combined with the production of other virulence factors such as lactic acid, may contribute to the pathogenesis of this bacterium. In the present study, the detection of genes encoding mutacin types I/III, II and IV was performed by PCR with specific primers to ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.45870-0
更新日期：2005-06-01 00:00:00
abstract：:Acinetobacter baumannii, Acinetobacter nosocomialis and Acinetobacter pittii are clinically relevant members of the Acinetobacter calcoaceticus-A. baumannii (Acb) complex and important nosocomial pathogens. These three species are genetically closely related and phenotypically similar; however, they differ in their ep...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.071712-0
更新日期：2014-09-01 00:00:00
abstract：PURPOSE:Listeria monocytogenes is a genetically heterogeneous species, which is divided into evolutionary lineages and clonal complexes (CCs). Not all L. monocytogenes isolates are equally likely to cause disease, with CC1, and in particular sequence type (ST) 1, being the most prevalent complex in human and ruminant i...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000529
更新日期：2017-07-01 00:00:00
abstract：:Meticillin-resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen in Pakistan and is emerging in the community. This is one of the first reports of the prevalent genotypes of MRSA in both hospital and community settings in Pakistan. Isolates collected in 2006-2007 were characterized by PFGE, staphylococ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.022707-0
更新日期：2011-01-01 00:00:00
abstract：:Cutaneous leishmaniasis (CL), a neglected tropical disease, is reported to be prevalent in tribal villages located in the Agasthyamala Biosphere Reserve forests of Western Ghats, Kerala state, India. We carried out an investigation to characterize the species of Leishmania parasites involved in these infections preval...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.076695-0
更新日期：2015-02-01 00:00:00
abstract：:Chlamydia trachomatis results in tubal factor infertility in some women. Diagnosis of this tubal infertility is difficult and typically involves laparoscopy or hysterosalpingography to detect the tubal blockages. Numerous serological tests have been developed; however, they are presently not used for diagnosis without...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000311
更新日期：2016-09-01 00:00:00
abstract：:The ability of Proteus mirabilis to produce the bacteriocin, proticine 3, is found almost exclusively in strains that cause severe infections of the upper urinary tract. Proticine 3 was purified from lysates of mitomycin C-induced cultures. Biological activity was associated with structures resembling bacteriophage ta...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-16-3-323
更新日期：1983-08-01 00:00:00
abstract：:The influence of six immunosuppressive agents on the occurrence of endogenous bacteraemia in mice was evaluated. The mortality rates in conventional ddY mice given cyclophosphamide (CY), fluorouracil (5-FU), methotrexate (MTX), cisplatin (CDDP) or FK-506 intraperitoneally, or dexamethasone (DXM) subcutaneously were 70...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-42-3-181
更新日期：1995-03-01 00:00:00
abstract：:A slide agglutination technique identifying 10 serogroups of Clostridium difficile (A,B,C,D,F,G,H,I,K and X) has been described previously. In this study, we have used the hamster to compare the ability of the 10 serogroup reference strains to colonise and produce disease. Groups of four hamsters were each given a sin...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-33-2-85
更新日期：1990-10-01 00:00:00
abstract：:Toxoplasma gondii infection in humans is routinely assessed by serological means. Here, the authors attempted to compare the response of different Toxoplasma strains to serological tests and to evaluate the antigenic profiles of the RH and RH Ankara (TRH) strains with Western blotting. Anti-Toxoplasma IgG antibodies o...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.46712-0
更新日期：2006-10-01 00:00:00
abstract：:The production of toxins A and B by Clostridium difficile was greatly enhanced under biotin-limited conditions, in which a 140-kDa protein was expressed strongly. Gene cloning revealed that this protein was a homologue of formylglycinamidine ribonucleotide synthetase (FGAM synthetase, EC 6.3.5.3), which is known as Pu...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/0022-1317-51-1-34
更新日期：2002-01-01 00:00:00
abstract：PURPOSE:In this study, we aimed to investigate the genomic characteristics and evolution of pathogenicity islands of an enteropathogenic Escherichia coli (EPEC) strain, and to obtain a transcriptional profile of EPEC under different concentrations of ciprofloxacin using microarray analysis. METHODOLOGY:The complete EP...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000790
更新日期：2018-09-01 00:00:00
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journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.45613-0
更新日期：2004-11-01 00:00:00
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journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.037036-0
更新日期：2012-02-01 00:00:00
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journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-31-2-125
更新日期：1990-02-01 00:00:00
abstract：:Inhalational anthrax is the most severe form of anthrax. It has been shown in small-animal and non-human primate models that relatively large pools of ungerminated Bacillus anthracis spores can remain within the alveolar spaces for days to weeks post-inhalation or until transported to areas more favourable for germina...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.008656-0
更新日期：2009-06-01 00:00:00
abstract：:Intestinal absorption of arbutin (p-hydroxyphenyl-beta-glucoside), a non-metabolised analogue of d-glucose, and net flux of water out of the small intestinal lumen were found to be decreased in vitro in rats fed with Candida albicans. In rats on a protein-deficient diet and fed C. albicans there was net secretion of w...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-13-1-103
更新日期：1980-02-01 00:00:00
abstract：:To elucidate whether Chlamydia trachomatis and C. pneumoniae infections occur to a significant extent in monocytes-macrophages, the human monocytic cell line, U-937, was infected with C. trachomatis L2 or C. pneumoniae TW-183. Chlamydial DNA and genus-specific antigens of the lipopolysaccharides (LPS) in epitopes of t...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-42-3-191
更新日期：1995-03-01 00:00:00
abstract：:Rickettsia conorii is endemic in Greece, though only a few cases of infection have been published to date. The case of a 58-year-old man from northern Greece with a severe form of Mediterranean spotted fever and rapid neurological deterioration is presented here. The patient received antibiotic treatment with doxycycl...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.46337-0
更新日期：2006-03-01 00:00:00
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journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-48-6-597
更新日期：1999-06-01 00:00:00
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journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-25-1-7
更新日期：1988-01-01 00:00:00
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journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-45-2-138
更新日期：1996-08-01 00:00:00
abstract：PURPOSE:This paper aims to evaluate the antimicrobial resistance of Esherichia coli isolates from children under 5 years old, with and without diarrhoea, who were hospital outpatients in Culiacan, Sinaloa, Mexico. It also looks at the antimicrobial activity of fruit extracts against selected multidrug-resistant (MDR) E...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000548
更新日期：2017-07-01 00:00:00
abstract：:Burkholderia pseudomallei is isolated frequently from the soil in regions where the disease melioidosis occurs. However, recent surveys in Thailand have shown that the frequency of isolation of the organism from soil samples is not directly related to the incidence of melioidosis in an area. To determine whether strai...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-46-7-565
更新日期：1997-07-01 00:00:00
abstract：:In this study, we investigated the in vitro antifungal effects of itraconazole/voriconazole (ITR/VRC) alone and in combination with tetrandrine (TET) against 23 clinical isolates of A. fumigatus using a chequerboard microdilution method. The dynamic antifungal effects of TET with ITR/VRC against A. fumigatus were asse...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000120
更新日期：2015-09-01 00:00:00
abstract：:Effects on growth and toxin A production of sub-MIC concentrations of six different antibiotics were investigated in three strains of Clostridium difficile: reference strain NCTC 11223, a fully sequenced strain (630) and a locally endemic isolate (strain 338a). The antibiotics chosen for investigation were the agents ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.05387-0
更新日期：2003-12-01 00:00:00
abstract：:Six-week-old lambs were inoculated orally with 10(9) cfu of an antibiotic-resistance marked four-strain mixture of enterohaemorrhagic Escherichia coli (EHEC) O157:H7 to investigate faecal excretion and intestinal colonisation. In the first experiment, three E. coli O157:H7 isolates were not detected in the faeces of a...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/0022-1317-51-12-1032
更新日期：2002-12-01 00:00:00
abstract：:Anaplasma phagocytophilum, an agent of human granulocytic anaplasmosis, infects neutrophils and causes an emerging tickborne febrile disease. The genome of this bacterium contains a large number of p44/msp2-related genes encoding 44 kDa major outer-membrane proteins, and it is known that a specific p44/msp2 gene is pr...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.011775-0
更新日期：2009-12-01 00:00:00
abstract：:Listeria monocytogenes cells are ingested by protozoa such as Acanthamoeba sp. or Tetrahymena pyriformis. However, they are not killed, but survive within the protozoa and may multiply intracellularly. The protozoa are lysed within about 8 days, releasing viable L. monocytogenes. No co-existence was observed between L...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-33-1-51
更新日期：1990-09-01 00:00:00
abstract：:A serial study of rotavirus infection in gnotobiotic piglets is described. They were infected when 7 days old and the course of infection was followed for 21 days, by immunofluorescence and histological examinations of the intestinal epithelium and by titration of the virus content of the gut lumen. There appeared to ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-11-3-325
更新日期：1978-08-01 00:00:00
