Abstract:
:Rhodococcus equi is an opportunistic human pathogen associated with immunosuppressed people. While the interaction of R. equi with macrophages has been comprehensively studied, little is known about its interactions with non-phagocytic cells. Here, we characterized the entry process of this bacterium into human lung epithelial cells. The invasion is inhibited by nocodazole and wortmannin, suggesting that the phosphatidylinositol 3-kinase pathway and microtubule cytoskeleton are important for invasion. Pre-incubation of R. equi with a rabbit anti-R. equi polyclonal antiserum resulted in a dramatic reduction in invasion. Also, the invasion process as studied by immunofluorescence and scanning electron microscopy indicates that R. equi make initial contact with the microvilli of the A549 cells, and at the structural level, the entry process was observed to occur via a zipper-like mechanism. Infected lung epithelial cells upregulate the expression of cytokines IL-8 and IL-6 upon infection. The production of these pro-inflammatory cytokines was significantly enhanced in culture supernatants from cells infected with non-mucoid plasmid-less strains when compared with cells infected with mucoid strains. These results demonstrate that human airway epithelial cells produce pro-inflammatory mediators against R. equi isolates.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Remuzgo-Martínez S,Pilares-Ortega L,Álvarez-Rodríguez L,Aranzamendi-Zaldunbide M,Padilla D,Icardo JM,Ramos-Vivas Jdoi
10.1099/jmm.0.056234-0subject
Has Abstractpub_date
2013-08-01 00:00:00pages
1144-1152issue
Pt 8eissn
0022-2615issn
1473-5644journal_volume
62pub_type
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