The role of HSV-induced Fc- and C3b (i)-receptors in bacterial adherence.

Abstract:

:Herpes simplex virus type-1 (HSV-1) induces Fc- and C3b(i)-receptors on infected cells. The role of these receptors in bacterial superinfection was studied by comparing the adherence of non-opsonised and opsonised bacteria to HSV-infected and non-infected HEp-2 cells. A flow cytometric adherence assay, based on the fluorescent quantitation of FITC-labelled bacteria, was developed. Opsonisation of Staphylococcus epidermidis with human serum, resulted in a marked increase in adherence to HSV-infected cells and revealed a role for C3b(i)R- and FcR-mediated adhesion. However, the enhanced adherence never exceeded the level of attachment to non-infected cells. Increased adherence of other pathogenic bacteria, including Escherichia coli, Streptococcus pneumoniae, Haemophilus influenzae and Pseudomonas aeruginosa was not observed, indicating that the HSV-receptors play a minor role in secondary infections. Bacterial adhesion factors such as the fimbriae of E. coli played a more dominant role in the adherence of bacteria to HSV-infected cells.

journal_name

J Med Microbiol

authors

De Graaf-Miltenburg LA,Van Vliet KE,Ten Hagen TL,Verhoef J,Van Strijp JA

doi

10.1099/00222615-40-1-48

subject

Has Abstract

pub_date

1994-01-01 00:00:00

pages

48-54

issue

1

eissn

0022-2615

issn

1473-5644

journal_volume

40

pub_type

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