Abstract:
:Interaction of human polymorphonuclear leucocytes (PMNL) and influenza virus (IFV) was studied in vitro. At 0 degree C, the viral particles were bound extensively to the surface of the PMNL membrane with a ratio of about 1000 virus particles to a single PMNL. The binding was sensitive to neuraminidase, suggesting attachment through sialo-compound receptors. At 37 degrees C, the virus particles disappeared rapidly from the cell surface, about half of them being eluted and the remainder being endocytosed into the cytoplasmic vesicles. Immuno-gold electronmicroscopy suggests that the virus particles are ingested into phagosomal vesicles and lysed.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Yamamoto K,Suzuki K,Suzuki K,Mizuno Sdoi
10.1099/00222615-28-3-191subject
Has Abstractpub_date
1989-03-01 00:00:00pages
191-8issue
3eissn
0022-2615issn
1473-5644journal_volume
28pub_type
杂志文章abstract::Mycobacterium bovis is a pathogen of cattle. The unpasteurized milk of affected cattle is a source of infection in humans. Despite the screening of cattle and the pasteurization of milk, M bovis has not been eradicated. A high index of clinical suspicion is needed in symptomatic patients with a history of possible exp...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.020511-0
更新日期:2010-11-01 00:00:00
abstract::Immunoblotting sera from cases of Streptococcus mutans or S. sobrinus endocarditis against an extract from S. sobrinus strain MUCOB 263 had identified three immunodominant antigenic bands at 190, 200 and 220 kDa. A lambda ZAPII DNA library was produced from the sheared genomic DNA of S. sobrinus MUCOB 263 and six iden...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-40-5-330
更新日期:1994-05-01 00:00:00
abstract::The purpose of this study was to evaluate the anti-chlamydial activities in vitro of liposome-encapsulated doxycycline (Dox) and tetracycline (Tet) in comparison with free Dox and Tet. Dox and Tet encapsulated in cationic (CAL), anionic (ANL) and neutral (NTL) liposomes by sonication, were quantified by high-performan...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-48-7-689
更新日期:1999-07-01 00:00:00
abstract:PURPOSE:Leptospira interrogans serogroup Icterohaemorrhagiae strains have been described as causing disease in both humans and animals and as being present worldwide. Icterohaemorrhagiae and Copenhageni serovars are known to cause severe disease in their hosts, and zoonotic outbreaks have been described. The genetic si...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000806
更新日期:2018-09-01 00:00:00
abstract::A novel aminopeptidase was purified by high performance liquid chromatography from a cytosoluble 100,000 g extract of Candida albicans on the basis of its ability to cleave L-arginine 7-amino-4-methylcoumarin. The purification factor was 36 and the yield was 20%. The native enzyme had a mol. wt of 52 kDa as demonstrat...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-43-4-282
更新日期:1995-10-01 00:00:00
abstract::Bacteroides gracilis is a gram-negative anaerobic bacillus which requires formate and fumarate for growth; it has been implicated in periodontal disease and serious infections of the head and neck. In this study, Bacteroides ureolyticus (NTU) medium was tested for its ability to allow the growth of B. gracilis and oth...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-35-5-294
更新日期:1991-11-01 00:00:00
abstract::Aspiration of oropharyngeal bacteria and fungi is occasionally suspected in patients with pneumonia. A patient with oral carcinoma underwent chemoradioimmunotherapy and, about 4 weeks from the start of the therapy, the patient suffered from severe oral mucositis induced by chemoradiotherapy, and candidal pneumonia was...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.45769-0
更新日期:2005-05-01 00:00:00
abstract:PURPOSE:The presence of alginate-overproducing (Alg+) strains of Pseudomonas aeruginosa in cystic fibrosis patients is indicative of chronic infection. The Alg+ phenotype is generally due to a mutation in the mucA gene, encoding an innermembrane protein that sequesters AlgT/U, the alginate-specific sigma factor. AlgT/U...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000752
更新日期:2018-08-01 00:00:00
abstract::The development of tuberculosis (TB) infection in humans depends on the mycobacterial strain and the human host, and is multigenically controlled in both. ATP ligation of P2X(7) receptors expressed on human macrophages infected with mycobacteria induces cell death and subsequent loss of intracellular bacterial viabili...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.47455-0
更新日期:2008-05-01 00:00:00
abstract::Growth of Candida albicans biofilms and production of extracellular matrix were monitored by dry weight, colorimetric and radioisotope assays, and by scanning electron microscopy. Under static incubation conditions synthesis of matrix material was minimal, but increased dramatically when developing biofilms were subje...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-47-3-253
更新日期:1998-03-01 00:00:00
abstract::Yeast identification and in vitro susceptibility testing provide helpful information for appropriate administration of antifungal treatments; however, few reports from the Latin American region have been published. The aim of this study was to identify the species present in isolates from bloodstream infections diagno...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.071167-0
更新日期:2014-06-01 00:00:00
abstract::The aim of this study was to evaluate the presence and prevalence of multidrug antibacterial resistance in Helicobacter pylori in Bulgaria from 2005 to 2008. The resistance in 828 untreated adults, 124 treated adults and 105 untreated children was, respectively, 26.5, 50.8 and 16.2% for metronidazole; 18.4, 45.2 and 1...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.009993-0
更新日期:2009-07-01 00:00:00
abstract::Effects on growth and toxin A production of sub-MIC concentrations of six different antibiotics were investigated in three strains of Clostridium difficile: reference strain NCTC 11223, a fully sequenced strain (630) and a locally endemic isolate (strain 338a). The antibiotics chosen for investigation were the agents ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.05387-0
更新日期:2003-12-01 00:00:00
abstract::Campylobacter jejuni is a frequent cause of infectious diarrhoea and is increasingly recognized as a trigger for late-onset complications. The poor standardization of commonly used serological tests might explain the conflicting results regarding the frequency of antecedent C. jejuni infections in defined patient grou...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46040-0
更新日期:2005-08-01 00:00:00
abstract::An ELISA was developed and evaluated as a method for detecting antibodies against glycosylated and deglycosylated histoplasmin (HMIN). Sera from patients with histoplasmosis, paracoccidioidomycosis, sporotrichosis, coccidioidomycosis, aspergillosis, cryptococcosis and healthy donors were tested by ELISA against purifi...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.05469-0
更新日期:2004-06-01 00:00:00
abstract::Campylobacter strains isolated from the same stool sample were characterised by determination of biochemical properties and both heat-labile (Lior) and heat-stable (Lauwers) serotypes. In six of 60 campylobacter-infected stools, two or three strains differing in Lior-serotype were isolated from the same stool. In four...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-38-1-3
更新日期:1993-01-01 00:00:00
abstract::Experiments were performed to determine whether a modern flow cytometer could be used to study bacterial populations in suspension, with particular reference to their morphological characteristics and their responses to antibiotics. The FACScan, a commercial benchtop flow cytometer fitted with an air-cooled laser, des...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-39-2-147
更新日期:1993-08-01 00:00:00
abstract:PURPOSE:Multidrug-resistant Klebsiella pneumoniae is a common nosocomial pathogen that plays an important role in ventilator-associated pneumonia (VAP). This study aimed to define the clonal relatedness of K. pneumoniae strains isolated from paediatric VAP in addition to those isolated from environmental samples. METH...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000473
更新日期:2017-05-01 00:00:00
abstract::Pseudomonas aeruginosa is an opportunistic pathogen that poses a threat in clinical settings due to its intrinsic and acquired resistance to a wide spectrum of antibiotics. Additionally, the presence of a subpopulation of cells surviving high concentrations of antibiotics, called persisters, makes it virtually impossi...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.019703-0
更新日期:2011-03-01 00:00:00
abstract::Bedaquiline (BDQ) has been proven to be effective in the treatment of multidrug-resistant tuberculosis. We hypothesized that BDQ could be a potential agent to treat nontuberculous mycobacterial (NTM) infection. The objective of this study was to evaluate the in vitro activity of BDQ against rapidly growing mycobacteri...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000537
更新日期:2017-08-01 00:00:00
abstract::Three specimens from 111 asymptomatic male partners of infertile couples attending the Department of Urology in Amiens, France, were examined by the PCR COBAS AMPLICOR test (Roche Molecular Diagnostics) for the presence of Chlamydia trachomatis. The specimens analysed were: first void urine (FVU), urine obtained after...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.45641-0
更新日期:2004-10-01 00:00:00
abstract:PURPOSE:Antimicrobial resistance (AMR) is of increasing global concern, threatening to undermine recent progress in reducing child and neonatal mortality. Repurposing older antimicrobials is a prominent strategy to combat multidrug-resistant sepsis. A potential agent is fosfomycin, however, there is scarce data regardi...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000973
更新日期:2019-05-01 00:00:00
abstract::PCR-based detection of Mucorales species could improve diagnosis of suspected invasive fungal infection, leading to a better patient outcome. This study describes two independent probe-based real-time PCR tests for detection of clinically relevant Mucorales, targeting specific fragments of the 18S and the 28S rRNA gen...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000218
更新日期:2016-03-01 00:00:00
abstract::A down-flow immunoassay has been developed to detect serum mannan antigens, and the test was recently marketed as the Unimedi Candida monotest. Using 251 serum samples from 105 patients with candidaemia, a comparison of the Unimedi Candida monotest with the Cand-Tec latex agglutination test and 2 microplate enzyme imm...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46314-0
更新日期:2006-05-01 00:00:00
abstract::The aim of the present study was to correlate molecular evidence of the presence of Helicobacter pylori in gastric biopsy samples, based on analysis of 16S rDNA, vacuolating toxin (vacA), urease A (ureA) and cagA genes, with the clinical, histological and serological findings in patients with H. pylori-associated gast...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-47-8-695
更新日期:1998-08-01 00:00:00
abstract::Feral pigeons (Columba livia) are commonly infected with Chlamydia psittaci, the agent of psittacosis in humans. To assess the risk of zoonosis posed by feral pigeons in the urban environment, we determined the prevalence of Chlamydia psittaci by detection of the outer-membrane protein A (ompA) gene of this pathogen i...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.034025-0
更新日期:2012-02-01 00:00:00
abstract::Leclercia adecarboxylata is a rarely reported human pathogen, most commonly affecting immunocompromised individuals. In reported cases of immunocompetent patients infected with this organism, it is seen exclusively in the context of polymicrobial infections. We report here the case of an abscess in an immunocompetent ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.47673-0
更新日期:2008-07-01 00:00:00
abstract::A commercial method (RAPIDEC ur; bioMérieux UK Ltd, Basingstoke) and an in-house scheme based on conventional tests were compared for the identification of 179 commonly encountered gram-negative urinary pathogens. The methods identified correctly to genus level 97% and 84% of organisms, respectively. RAPIDEC ur was su...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-43-1-72
更新日期:1995-07-01 00:00:00
abstract::The response of Legionella pneumophila to antibiotics that inhibit cell-wall, protein and DNA synthesis was examined by electronmicroscopy, MIC estimations and viable counts. Ampicillin, cefotaxime, methicillin, erythromycin, rifampicin and ciprofloxacin, each used separately at 20 times their respective MIC values, s...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-31-1-37
更新日期:1990-01-01 00:00:00
abstract::Antibodies specific for rubella virion polypeptide, VPI, secreted by clones of hybridoma cells or produced in rabbits in response to specific antigenic stimulation, located determinants for haemagglutination inhibiting (HI) and neutralising (Nt) antibodies on this envelope component. ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-17-1-105
更新日期:1984-02-01 00:00:00