Improved serodiagnosis of Campylobacter jejuni infections using recombinant antigens.

abstract：:The aim of this study was to evaluate the toxigenic status of circulating strains of Clostridium difficile in a large teaching hospital. Overall 220 isolates were studied of which 199 (90.5 %) produced both large clostridial toxins detected by conventional methods. Ten more strains (4.5 %) had toxin A and B genes dete...

journal_title：Journal of medical microbiology

authors： Alonso R,Martín A,Peláez T,Marín M,Rodríguez-Creixéms M,Bouza E

更新日期：2005-02-01 00:00:00

abstract：:PCR has proved superior to conventional blood culture for diagnosing bacteraemia in the presence of antibiotics. Nevertheless, even PCR might yield false-negative results if the template DNA were to be cleaved by serum DNAases after antibiotics had induced bacterial death. To evaluate the cleavage of bacterial templat...

journal_title：Journal of medical microbiology

authors： Heininger A,Ulrich M,Priebe G,Unertl K,Müller-Schauenburg A,Botzenhart K,Döring G

更新日期：2001-03-01 00:00:00

abstract：:Inhalational anthrax is the most severe form of anthrax. It has been shown in small-animal and non-human primate models that relatively large pools of ungerminated Bacillus anthracis spores can remain within the alveolar spaces for days to weeks post-inhalation or until transported to areas more favourable for germina...

journal_title：Journal of medical microbiology

authors： Cote CK,Bozue J,Twenhafel N,Welkos SL

更新日期：2009-06-01 00:00:00

abstract：:The role of Bacteroides fragilis in the pathogenesis of acute appendicitis was studied in 135 patients in four patient groups: normal (17); phlegmonous appendicitis (17); gangrenous appendicitis (75); and septic complications of appendicitis (26). Aerobic and anaerobic bacteria were isolated from all groups and member...

journal_title：Journal of medical microbiology

authors： Elhag KM,Alwan MH,Al-Adnani MS,Sherif RA

更新日期：1986-05-01 00:00:00

abstract：:The possession of any of eight different plasmids by Staphylococcus aureus strain 649--either singly or simultaneously (in no. 649MR)--caused changes in growth kinetics. Six of the plasmids caused an increase in exponential doubling time (by 8-25%), and most also altered the duration of the lag period. Strain 649MR wa...

journal_title：Journal of medical microbiology

authors： Lacey RW,Chopra I

更新日期：1975-02-01 00:00:00

abstract：:EDTA-released outer-membrane antigen complexes were prepared from 172 laboratory and reference strains and 43 fresh clinical or faecal isolates representing 20 species or subspecies of Bacteroides, together with 13 species of other genera. These antigens were titrated against antisera to whole, live cells of 21 specie...

journal_title：Journal of medical microbiology

authors： Poxton IR,Brown R,Collee JG

更新日期：1982-05-01 00:00:00

abstract：:Leptospirosis is a widespread zoonotic disease that affects all mammals in different parts of the world. Though there are many commercial kits available for the diagnosis of systemic leptospirosis, the nature of the antigen has not been described. Therefore, identification of a specific antigen is important. Since ocu...

journal_title：Journal of medical microbiology

authors： Priya CG,Bhavani K,Rathinam SR,Muthukkaruppan VR

更新日期：2003-08-01 00:00:00

abstract：:Enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) are members of the species Human enterovirus A, and are both major and independent aetiological agents of hand-foot-and-mouth disease. The human enterovirus (HEV) 5' untranslated region (UTR) is fundamentally important for efficient virus replication and for virulen...

journal_title：Journal of medical microbiology

authors： Zhou F,Kong F,Wang B,McPhie K,Gilbert GL,Dwyer DE

更新日期：2011-03-01 00:00:00

abstract：:Helicobacter pylori infection represents a key factor in the aetiology of various gastrointestinal diseases. H. pylori infection diagnosis is generally achieved using both invasive (e.g. biopsy of the gastric epithelium) and non-invasive methods. Therefore, cultivation on a growth medium becomes complex. Trypsin is a ...

journal_title：Journal of medical microbiology

authors： Peretz A,Paritsky M,Pastukh N,Koifman A,Brodsky D,Glyatman T,On A

更新日期：2015-06-01 00:00:00

abstract：:Certain bile acids inhibit the growth in vitro of organisms commonly found in the intestine and, in particular, anaerobic bacteria. As the importance of this effect in vivo has not been demonstrated, we investigated changes in intestinal flora in the rat after diversion of bile to the urinary bladder. Five days after ...

journal_title：Journal of medical microbiology

authors： Burke V,Stone DE,Beaman J,Gracey M

更新日期：1977-05-01 00:00:00

abstract：:Most past studies of cross-infection with Pseudomonas aeruginosa among cystic fibrosis (CF) patients in the UK suggest that it is a rare occurrence. However, two recent reports of highly transmissible strains in patients in regional centres in England (Liverpool and Manchester) have raised questions as to the extent o...

journal_title：Journal of medical microbiology

authors： Scott FW,Pitt TL

更新日期：2004-07-01 00:00:00

abstract：:Trichoderma longibrachiatum is an emerging pathogen in immunocompromised patients. We report a case of Trichoderma post-operative mediastinitis and peritonitis in a child with complex congenital cardiac disease and functional asplenia. The patient was treated unsuccessfully, initially with caspofungin alone followed b...

journal_title：Journal of medical microbiology

authors： Santillan Salas CF,Joshi AY,Dhiman N,Banerjee R,Huskins WC,Wengenack NL,Henry NK

更新日期：2011-12-01 00:00:00

abstract：:Toxoplasma gondii infection in humans is routinely assessed by serological means. Here, the authors attempted to compare the response of different Toxoplasma strains to serological tests and to evaluate the antigenic profiles of the RH and RH Ankara (TRH) strains with Western blotting. Anti-Toxoplasma IgG antibodies o...

journal_title：Journal of medical microbiology

authors： Delibas SB,Ertabaklar H,Ertug S

更新日期：2006-10-01 00:00:00

abstract：:A strain of Staphylococcus aureus (M7) contains a transmissible element determining production of penicillinase, and resistance to cadmium ions, neomycin, streptomycin and kanamycin (CPNS). This element was transferred either in toto or in fragments at low frequency from strain M7. The fragment (NS) possesses features...

journal_title：Journal of medical microbiology

authors： Lacey RW

更新日期：1979-08-01 00:00:00

abstract：PURPOSE:Multidrug-resistant Klebsiella pneumoniae is a common nosocomial pathogen that plays an important role in ventilator-associated pneumonia (VAP). This study aimed to define the clonal relatedness of K. pneumoniae strains isolated from paediatric VAP in addition to those isolated from environmental samples. METH...

journal_title：Journal of medical microbiology

authors： Mohamed ER,Aly SA,Halby HM,Ahmed SH,Zakaria AM,El-Asheer OM

更新日期：2017-05-01 00:00:00

abstract：:Brachyspira pilosicoli is a potentially zoonotic anaerobic intestinal spirochaete that is one of several species causing avian intestinal spirochaetosis. The aim of this study was to develop a reproducible model of infection in point-of-lay chickens and compare the virulence of two strains of B. pilosicoli in a model ...

journal_title：Journal of medical microbiology

authors： Mappley LJ,Tchórzewska MA,Nunez A,Woodward MJ,La Ragione RM

更新日期：2013-02-01 00:00:00

abstract：:Early diagnosis of leptospirosis is important because severe leptospiral infection can run a fulminant course. The polymerase chain reaction (PCR) was evaluated for the detection of leptospires in clinical samples from patients with acute leptospiral infection. Blood and urine samples from 71 patients with leptospiros...

journal_title：Journal of medical microbiology

authors： Brown PD,Gravekamp C,Carrington DG,van de Kemp H,Hartskeerl RA,Edwards CN,Everard CO,Terpstra WJ,Levett PN

更新日期：1995-08-01 00:00:00

abstract：:Eight monoclonal antibodies (MAbs) were produced from mice immunised with whole cells of heat-killed Pasteurella multocida type A which had been cultured under iron-restricted conditions. The MAbs were selected by an enzyme-linked immunosorbent assay (ELISA) in which the antigen consisted of whole bacteria of the immu...

journal_title：Journal of medical microbiology

authors： Wijewardana TG,Wilson CF,Gilmour NJ,Poxton IR

更新日期：1990-12-01 00:00:00

abstract：:Clinical and bacteriological findings in seven cases of urinary tract infection with cysteine-requiring strains of Klebsiella are described. The organisms were isolated from patients with long-standing urinary tract abnormalities and grew as small (c. 1 mm) colonies on MacConkey agar. The organisms failed to grow in a...

journal_title：Journal of medical microbiology

authors： McIver CJ,Tapsall JW

更新日期：1988-07-01 00:00:00

abstract：:The characterization and tracking of pathogenic micro-organisms in the clinical laboratory and public health environment demand schemes that are easy to standardize and use, are automated and high-throughput, and provide portable data. A combined genomarkers approach for Salmonella enterica based on comparative sequen...

journal_title：Journal of medical microbiology

authors： Bishop C,Honisch C,Goldman T,Mosko M,Keng S,Arnold C,Gharbia S

更新日期：2012-11-01 00:00:00

abstract：:The closely related Clostridium novyi and Clostridium botulinum types C and D are of current interest because of their association with serious infections in injecting drug users (C. novyi type A) and equine and feline dysautonomias (C. botulinum types C/D). The species are defined by the major toxins they produce: th...

journal_title：Journal of medical microbiology

authors： Heffron A,Poxton IR

更新日期：2007-02-01 00:00:00

abstract：:Introduction. The emergence of novel strains of Vibrio cholerae O1 El Tor biotype has gained attention due to causing several epidemics around the world. Variant strains have evolved as a result of the acquisition of genes that confer extended virulence and pathogenicity.Aim. This study aimed to determine the presence...

journal_title：Journal of medical microbiology

authors： Mohanraj RS,Samanta P,Mukhopadhyay AK,Mandal J

更新日期：2020-03-01 00:00:00

abstract：:A series of 178 strains of Escherichia coli, highly resistant to trimethoprim, isolated from hospital patients and patients in the community between 1979 and 1983, was examined for the presence of Tn7 on a plasmid or on the chromosome only, by transposition to RP4 and restriction endonuclease digestion with Hind III. ...

journal_title：Journal of medical microbiology

authors： Kraft CA,Timbury MC,Platt DJ

更新日期：1986-09-01 00:00:00

abstract：:The agglutination of 218 clinical isolates and three ATCC type strains of "Streptococcus milleri" was tested with 25 different lectins from plants and fungi. An agglutination reaction with one or more lectins was observed with 42 isolates when the cells were untreated. After trypsinisation of the bacteria, 109 strains...

journal_title：Journal of medical microbiology

authors： Kellens JT,Jacobs JA,Peumans WJ,Stobberingh EE

更新日期：1994-07-01 00:00:00

abstract：PURPOSE:We aimed to evaluate a PCR-based technique for the diagnosis of mucormycosis and the identification of fungi from fresh tissue specimens in patients with rhino-orbito-cerebral-mucormycosis (ROCM). METHODOLOGY:Fifty cases of ROCM were included in the study. Conventional identification was performed using micros...

journal_title：Journal of medical microbiology

authors： Zaman K,Rudramurthy SM,Das A,Panda N,Honnavar P,Kaur H,Chakrabarti A

更新日期：2017-08-01 00:00:00

abstract：:Human brucellosis is a zoonotic disease which is endemic in Saudi Arabia. The aim of this study was to investigate the humoral immune responses and identify the target antigens that persist at different stages in human brucellosis during antibiotic therapy. To do this, an acute case of accidental nosocomial infection ...

journal_title：Journal of medical microbiology

authors： Kwaasi AA,Al-Mohanna FA,Nakeeb SM,Roberts GT,Al-Thawadi S,Hassan AY,Al-Hokail A,Elfaki MG

更新日期：2005-06-01 00:00:00

abstract：:The response of Legionella pneumophila to antibiotics that inhibit cell-wall, protein and DNA synthesis was examined by electronmicroscopy, MIC estimations and viable counts. Ampicillin, cefotaxime, methicillin, erythromycin, rifampicin and ciprofloxacin, each used separately at 20 times their respective MIC values, s...

journal_title：Journal of medical microbiology

authors： Rodgers FG,Tzianabos AO,Elliott TS

更新日期：1990-01-01 00:00:00

abstract：:Motile curved rods seen in vaginal secretions have been isolated on Columbia agar supplemented with 5% human blood and vitamin K. Growth occurred anaerobically and in 5% oxygen but not in more aerobic conditions. There were two distinct groups of these organisms, distinguishable by morphology, biochemical activity and...

journal_title：Journal of medical microbiology

authors： Sprott MS,Ingham HR,Pattman RS,Eisenstadt RL,Short GR,Narang HK,Sisson PR,Selkon JB

更新日期：1983-05-01 00:00:00

abstract：:The prevalence and genotype of Shiga-like toxins (SLTs) in Escherichia coli (O)157 strains from patients in Germany with haemolytic uraemic syndrome (HUS) were investigated. This was done by PCR amplification of the B-subunit genes with two primer pairs--one complementary to slt-IB, and the other homologous to both sl...

journal_title：Journal of medical microbiology

authors： Rüssmann H,Schmidt H,Heesemann J,Caprioli A,Karch H

更新日期：1994-05-01 00:00:00

abstract：:Globally, specific genotypes of Mycobacterium tuberculosis have been shown to dominate in patients, suggesting that these are more successful pathogens. One such genotype, the F15/LAM4/KZN (KZN) family of M. tuberculosis, has predominated in KwaZulu-Natal, South Africa, since the early 1990s. This strain recently evol...

journal_title：Journal of medical microbiology

authors： Ashiru OT,Pillay M,Sturm AW

更新日期：2010-05-01 00:00:00