Cloning and sequencing the endocarditis immunodominant antigen of Streptococcus sobrinus strain MUCOB 263.

Abstract:

:Immunoblotting sera from cases of Streptococcus mutans or S. sobrinus endocarditis against an extract from S. sobrinus strain MUCOB 263 had identified three immunodominant antigenic bands at 190, 200 and 220 kDa. A lambda ZAPII DNA library was produced from the sheared genomic DNA of S. sobrinus MUCOB 263 and six identical positive clones were identified when this library was screened with serum from a patient with endocarditis caused by a bacterium from the mutans group of streptococci. On subcloning and sequencing, a protein containing 1548 amino acids was identified with a 99.2% homology to the SpaA antigen of S. sobrinus and 68.4% homology to the PAc antigen of S. mutans.

journal_name

J Med Microbiol

authors

Brooks W,Burnie JP

doi

10.1099/00222615-40-5-330

subject

Has Abstract

pub_date

1994-05-01 00:00:00

pages

330-7

issue

5

eissn

0022-2615

issn

1473-5644

journal_volume

40

pub_type

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