Prevalent genotypes of meticillin-resistant Staphylococcus aureus: report from Pakistan.

Abstract:

:Meticillin-resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen in Pakistan and is emerging in the community. This is one of the first reports of the prevalent genotypes of MRSA in both hospital and community settings in Pakistan. Isolates collected in 2006-2007 were characterized by PFGE, staphylococcal cassette chromosome mec (SCCmec) typing and multilocus sequence typing (MLST). PFGE identified nine pulsotypes, the majority of isolates belonging to pulsotypes A (n=70) and B (n=38), which were predominant among hospital-onset MRSA (HO-MRSA) and community-onset MRSA (CO-MRSA) isolates, respectively. Among the HO-MRSA isolates, variants of SCCmec type III were prevalent, whilst SCCmec type IV or variants were predominant in the CO-MRSA isolates. MLST identified two principal sequence types, ST8 and ST239. An association was observed between ST8, PFGE pulsotype B and SCCmec type IV in the CO-MRSA (ST8-MRSA-IV). Similarly, ST239, PFGE pulsotype A and SCCmec type III were associated with HO-MRSA (ST239-MRSA-III). Therefore, the prevalent genotypes circulating in Pakistan at the time of study were ST8-MRSA-IV and ST239-MRSA-III in the community and hospital settings, respectively. A set of HO-MRSA isolates collected in 1997 were characterized by PFGE and SCCmec typing for comparison. The isolates belonged to two PFGE pulsotypes (A, n=28; B, n=11) and contained just two SCCmec types. These results suggest that an increase in genetic diversity occurred over the period 1997-2007 as a result of either microevolution or the importation of strains from surrounding areas.

journal_name

J Med Microbiol

authors

Zafar A,Stone M,Ibrahim S,Parveen Z,Hasan Z,Khan E,Hasan R,Wain J,Bamford K

doi

10.1099/jmm.0.022707-0

subject

Has Abstract

pub_date

2011-01-01 00:00:00

pages

56-62

issue

Pt 1

eissn

0022-2615

issn

1473-5644

journal_volume

60

pub_type

杂志文章
  • Variation in the persistence of Escherichia coli O157:H7 in experimentally inoculated 6-week-old conventional lambs.

    abstract::Six-week-old lambs were inoculated orally with 10(9) cfu of an antibiotic-resistance marked four-strain mixture of enterohaemorrhagic Escherichia coli (EHEC) O157:H7 to investigate faecal excretion and intestinal colonisation. In the first experiment, three E. coli O157:H7 isolates were not detected in the faeces of a...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-51-12-1032

    authors: Cookson AL,Wales AD,Roe JM,Hayes CM,Pearson GR,Woodward MJ

    更新日期:2002-12-01 00:00:00

  • Comparison of two automated instruments for Epstein-Barr virus serology in a large adult hospital and implementation of an Epstein-Barr virus nuclear antigen-based testing algorithm.

    abstract::Serology remains the mainstay for diagnosis of Epstein-Barr virus (EBV) infection. This study compared two automated platforms (BioPlex 2200 and Architect i2000SR) to test three EBV serological markers: viral capsid antigen (VCA) immunoglobulins of class M (IgM), VCA immunoglobulins of class G (IgG) and EBV nuclear an...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000616

    authors: Al Sidairi H,Binkhamis K,Jackson C,Roberts C,Heinstein C,MacDonald J,Needle R,Hatchette TF,LeBlanc JJ

    更新日期:2017-11-01 00:00:00

  • Nosocomial infection with Asaia lannensis in two paediatric patients with idiopathic dilated cardiomyopathy.

    abstract::This is the first report, to our knowledge, of two temporally and geographically related nosocomial Asaia lannensis infections in a paediatric setting. Two patients with idiopathic dilated cardiomyopathy awaiting cardiac transplantation developed bacteraemia during their hospital stay. The physical location of both pa...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.019067-0

    authors: Juretschko S,Beavers-May TK,Stovall SH

    更新日期:2010-07-01 00:00:00

  • Wild birds as biological indicators of environmental pollution: antimicrobial resistance patterns of Escherichia coli and enterococci isolated from common buzzards (Buteo buteo).

    abstract::A total of 36 Escherichia coli and 31 enterococci isolates were recovered from 42 common buzzard faecal samples. The E. coli isolates showed high levels of resistance to streptomycin and tetracycline. The following resistance genes were detected: bla(TEM) (20 of 22 ampicillin-resistant isolates), tet(A) and/or tet(B) ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.038364-0

    authors: Radhouani H,Poeta P,Gonçalves A,Pacheco R,Sargo R,Igrejas G

    更新日期:2012-06-01 00:00:00

  • Roles of pyruvate dehydrogenase and branched-chain α-keto acid dehydrogenase in branched-chain membrane fatty acid levels and associated functions in Staphylococcus aureus.

    abstract:PURPOSE:Membrane fluidity to a large extent is governed by the presence of branched-chain fatty acids (BCFAs). Branched-chain α-keto acid dehydrogenase (BKD) is the key enzyme in BCFA synthesis. A Staphylococcus aureus BKD-deficient strain still produced substantial levels of BCFAs. Pyruvate dehydrogenase (PDH) with st...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000707

    authors: Singh VK,Sirobhushanam S,Ring RP,Singh S,Gatto C,Wilkinson BJ

    更新日期:2018-04-01 00:00:00

  • Antimicrobial and immunomodulatory effect of clarithromycin on macrolide-resistant Mycoplasma pneumoniae.

    abstract::Macrolide antibiotics are frequently administered to treat mycoplasmal pneumonia. However, macrolide-resistant Mycoplasma pneumoniae has recently been isolated from clinical specimens in Japan. Clarithromycin (CAM) is a 14-membered-ring macrolide that has host immunomodulatory activity. Here, we established a gnotobio...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.014191-0

    authors: Kurata S,Taguchi H,Sasaki T,Fujioka Y,Kamiya S

    更新日期:2010-06-01 00:00:00

  • In-vitro characterisation of the phagocytosis and fate of anthrax spores in macrophages and the effects of anti-PA antibody.

    abstract::Antibodies (Abs) to the protective antigen (PA) component of the anthrax toxins have anti-spore as well as anti-toxin activities. Anti-PA antisera and purified anti-PA Abs enhance the phagocytosis by murine-derived macrophages (MQs) of spores of the Ames and Sterne strains and retard the germination of extracellular s...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-51-10-821

    authors: Welkos S,Friedlander A,Weeks S,Little S,Mendelson I

    更新日期:2002-10-01 00:00:00

  • A large food-borne outbreak of campylobacteriosis in kindergartens and primary schools in Pescara, Italy, May-June 2018.

    abstract::Introduction. In May-June 2018, an outbreak of campylobacteriosis involved students and school staff from kindergartens and primary schools in Pescara, southern Italy.Aim. We present details of the epidemiological and microbiological investigation, and the findings of the analytical study, as well as the implemented c...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001262

    authors: Sorgentone S,Busani L,Calistri P,Robuffo G,Bellino S,Acciari V,Ferri M,Graziani C,Antoci S,Lodi F,Alfonsi V,Cammà C,Fazii P,Andrianou X,Cito F,Lombardi G,Centorotola G,D'Amario M,D'Alterio N,Savini V,De Massis F,

    更新日期:2021-01-21 00:00:00

  • Two different types of humoral immune response to Actinobacillus actinomycetemcomitans in high-responder periodontitis patients.

    abstract::Actinobacillus actinomycetemcomitans is considered to be an aetiological agent in various forms of periodontitis, with serotype b-specific carbohydrate being the immunodominant antigen of A. actinomycetemcomitans Y4 in high-responder patients. Lipopolysaccharide (LPS) of the organism may also be an important antigen. ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-47-7-569

    authors: Nakashima K,Usui C,Koseki T,Nishihara T,Ishikawa I

    更新日期:1998-07-01 00:00:00

  • Nucleotide sequence-based typing of meningococci directly from clinical samples.

    abstract::The unpredictable characteristics of meningococcal disease (MD) make outbreaks complicated to monitor and consequently lead to high levels of public anxiety. Traditional molecular techniques have been utilized in order to understand better the epidemiology of MD, but some have disadvantages such as being highly specia...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.05078-0

    authors: Diggle MA,Bell CM,Clarke SC

    更新日期:2003-06-01 00:00:00

  • Loss of protective antigen, histamine-sensitising factor and envelope polypeptides in cultural variants of Bordetella pertussis.

    abstract::Five Bordetella pertussis strains of phase I were grown in conventional casamino-acid medium and in media modified by adding high concentrations of MgSO4 or nicotinic acid. Cells grown in high-magnesium media (in the C-mode) had only about 4% of the protective antigen (PA) and 6% of the histamine-sensitising factor (H...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-9-1-89

    authors: Wardlaw AC,Parton R,Hooker MJ

    更新日期:1976-02-01 00:00:00

  • Molecular epidemiology of an outbreak of multiresistant Klebsiella pneumoniae in a Tunisian neonatal ward.

    abstract::During the first quarter of 1996, a major outbreak of clinical infection caused by multiresistant Klebsiella pneumoniae (MRKP) occurred in the neonatal ward of the 'Maternité Wassila Bourguiba' in Tunis, Tunisia. In total, 32 isolates of MRKP, comprising 23 clinical isolates and nine surveillance isolates, were recove...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.04981-0

    authors: Ben-Hamouda T,Foulon T,Ben-Cheikh-Masmoudi A,Fendri C,Belhadj O,Ben-Mahrez K

    更新日期:2003-05-01 00:00:00

  • Characterization of the clonal subpopulation Fiocruz L1-130 of Leptospira interrogans in rats and dogs from Brazil.

    abstract:PURPOSE:Leptospira interrogans serogroup Icterohaemorrhagiae strains have been described as causing disease in both humans and animals and as being present worldwide. Icterohaemorrhagiae and Copenhageni serovars are known to cause severe disease in their hosts, and zoonotic outbreaks have been described. The genetic si...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000806

    authors: Jaeger LH,Pestana CP,Carvalho-Costa FA,Medeiros MA,Lilenbaum W

    更新日期:2018-09-01 00:00:00

  • Virulent Toxoplasma gondii strain RH promotes T-cell-independent overproduction of proinflammatory cytokines IL12 and gamma-interferon.

    abstract::The aim of this study was the analysis of the cytokine response in BALB/c mice infected with the highly virulent RH or the weakly virulent Beverley strains of Toxoplasma gondii. Analysis of cytokine messages showed increased expression of IL12, IFN-gamma and TNF-alpha, but not IL4 mRNAs in spleen cells after infection...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.04860-0

    authors: Nguyen TD,Bigaignon G,Markine-Goriaynoff D,Heremans H,Nguyen TN,Warnier G,Delmee M,Warny M,Wolf SF,Uyttenhove C,Van Snick J,Coutelier JP

    更新日期:2003-10-01 00:00:00

  • Development of a multiplex-PCR for direct detection of the genes for enterotoxin B and C, and toxic shock syndrome toxin-1 in Staphylococcus aureus isolates.

    abstract::As well as conventional methods such as immunodiffusion, ELISA, or agglutination for the detection of toxin production in Staphylococcus aureus, amplification techniques like PCR allow a very sensitive and specific identification of the genes responsible for enterotoxin B and C, and TSST-1 production. These toxins mig...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-47-4-335

    authors: Schmitz FJ,Steiert M,Hofmann B,Verhoef J,Hadding U,Heinz HP,Köhrer K

    更新日期:1998-04-01 00:00:00

  • Inhibition of streptolysin O by allicin - an active component of garlic.

    abstract::Streptolysin O (SLO) is a potent cytolytic toxin produced by almost all strains of group A streptococci and is considered an important virulence factor for this organism. In this study we investigated the effect of allicin and aqueous garlic extracts on the haemolytic activity of SLO. All tested materials potentially ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.019539-0

    authors: Arzanlou M,Bohlooli S

    更新日期:2010-09-01 00:00:00

  • Comparative viral frequency in Mexican children under 5 years of age with and without upper respiratory symptoms.

    abstract::In Mexico, there is a lack of up-to-date published data that show viruses to be the main cause of acute respiratory infection (ARI). The objective of this study was to estimate the comparative viral frequency between children under the age of 5 years with and without ARI (n = 179 in each group) in a suburban community...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.05007-0

    authors: Manjarrez ME,Rosete DP,Rincón M,Villalba J,Cravioto A,Cabrera R

    更新日期:2003-07-01 00:00:00

  • Characterization of Shigella sonnei isolates from travel-associated cases in Japan.

    abstract::Shigella sonnei infection in industrialized countries is often associated with foreign travel. A total of 195 S. sonnei isolates in Japan, isolated from cases associated with foreign travel, were analysed by biotyping and molecular typing using PFGE and multilocus variable-number tandem-repeat analysis (MLVA); their a...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.011809-0

    authors: Izumiya H,Tada Y,Ito K,Morita-Ishihara T,Ohnishi M,Terajima J,Watanabe H

    更新日期:2009-11-01 00:00:00

  • Molecular characterization of enterovirus 71 and coxsackievirus A16 using the 5' untranslated region and VP1 region.

    abstract::Enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) are members of the species Human enterovirus A, and are both major and independent aetiological agents of hand-foot-and-mouth disease. The human enterovirus (HEV) 5' untranslated region (UTR) is fundamentally important for efficient virus replication and for virulen...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.025056-0

    authors: Zhou F,Kong F,Wang B,McPhie K,Gilbert GL,Dwyer DE

    更新日期:2011-03-01 00:00:00

  • Plasmid associated virulence properties of environmental isolates of Aeromonas hydrophila.

    abstract::The plasmid profiles, and their association with antimicrobial resistance, of 60 strains of Aeromonas hydrophila isolated from fish, shellfish and water were investigated. Only two strains were susceptible to all the antimicrobial agents tested; the highest incidences of resistance were to tetracycline (96.7%), prysta...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-35-5-264

    authors: Borrego JJ,Moriñigo MA,Martinez-Manzanares E,Bosca M,Castro D,Barja JL,Toranzo AE

    更新日期:1991-11-01 00:00:00

  • Gas-chromatographic lipid profiles in identification of currently known slowly growing environmental mycobacteria.

    abstract::Cellular fatty acid analysis by GLC is widely used in the species identification of mycobacteria. Combining mycolic acid cleavage products with shorter cellular fatty acids increases the informative value of the analysis. A key has been created to aid in the identification of all currently known slowly growing environ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.05113-0

    authors: Torkko P,Katila ML,Kontro M

    更新日期:2003-04-01 00:00:00

  • Candida auris: a comparison between planktonic and biofilm susceptibility to antifungal drugs.

    abstract::Introduction.Candida auris is a pathogenic yeast that mainly affects immunosuppressed patients and those with implanted medical devices. This pathogen also displays elevated resistance to common antifungals and high survival and spreading capacities. Since no antifungal breakpoints have yet been defined for this patho...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001036

    authors: Romera D,Aguilera-Correa JJ,Gadea I,Viñuela-Sandoval L,García-Rodríguez J,Esteban J

    更新日期:2019-09-01 00:00:00

  • Evaluation of the polymerase chain reaction for early diagnosis of leptospirosis.

    abstract::Early diagnosis of leptospirosis is important because severe leptospiral infection can run a fulminant course. The polymerase chain reaction (PCR) was evaluated for the detection of leptospires in clinical samples from patients with acute leptospiral infection. Blood and urine samples from 71 patients with leptospiros...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-43-2-110

    authors: Brown PD,Gravekamp C,Carrington DG,van de Kemp H,Hartskeerl RA,Edwards CN,Everard CO,Terpstra WJ,Levett PN

    更新日期:1995-08-01 00:00:00

  • Bacterial colonization of enamel in situ investigated using fluorescence in situ hybridization.

    abstract::Oral biofilms are one of the greatest challenges in dental research. The present study aimed to investigate initial bacterial colonization of enamel surfaces in situ using fluorescence in situ hybridization (FISH) over a 12 h period. For this purpose, bovine enamel slabs were fixed on buccal sites of individual splint...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.011213-0

    authors: Al-Ahmad A,Follo M,Selzer AC,Hellwig E,Hannig M,Hannig C

    更新日期:2009-10-01 00:00:00

  • Differential binding of Shiga toxin 2 to human and murine neutrophils.

    abstract::Shiga toxins (Stx1 and Stx2) are responsible for initiating haemolytic uraemic syndrome, a serious extraintestinal complication caused by enterohaemorrhagic Escherichia coli O157 : H7 infection in humans. Shiga toxins are classical AB(5)-type exotoxins, consisting of a globotriaosylceramide (Gb(3))-binding B subunit p...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.47282-0

    authors: Griener TP,Mulvey GL,Marcato P,Armstrong GD

    更新日期:2007-11-01 00:00:00

  • Prevalence and characterization of quinolone resistance in Laribacter hongkongensis from grass carp and Chinese tiger frog.

    abstract::Laribacter hongkongensis is a food-borne bacterium associated with community-acquired gastroenteritis and diarrhoea. Quinolone resistance was recently reported in bacterial isolates from aquatic products, but the molecular mechanisms for resistance were still unknown. In this study, a total of 157 L. hongkongensis str...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.059451-0

    authors: Chen DQ,Yang L,Luo YT,Mao MJ,Lin YP,Wu AW

    更新日期:2013-10-01 00:00:00

  • Depletion of lymphocytes, but not neutrophils, via apoptosis in a murine model of Vibrio vulnificus infection.

    abstract::Vibrio vulnificus causes severe sepsis in humans. There are several reports about the relationship between host immunity and bacterial growth in V. vulnificus infection. However, the effect on leukocytes of V. vulnificus infection in vivo has not been elucidated. A murine model of V. vulnificus infection was used to i...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45861-0

    authors: Kashimoto T,Ueno S,Hayashi H,Hanajima M,Yoshioka K,Yoshida K,Mutoh K,Susa N

    更新日期:2005-01-01 00:00:00

  • Investigations into the influence of host genetics on the predominant eubacteria in the faecal microflora of children.

    abstract::The eubacterial population was studied in faecal samples of related and unrelated children. Temporal temperature gradient gel electrophoresis (TTGE) provided a snapshot of the bacterial population and allowed calculation of the degree of similarity in the predominant faecal microflora of identical twin pairs, fraterna...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46189-0

    authors: Stewart JA,Chadwick VS,Murray A

    更新日期:2005-12-01 00:00:00

  • Mapping the genetic diversity within major clonal complexes of meticillin-resistant Staphylococcus aureus utilizing genome-wide fluorescent amplified fragment length polymorphism markers.

    abstract::The genetic diversity between major meticillin-resistant Staphylococcus aureus (MRSA) lineages was probed using fluorescent amplified fragment length polymorphism (FAFLP) as a random genome sampling tool. Genomic DNA was digested with endonucleases BglII and Csp6I and a subset of the restricted fragments were amplifie...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.049429-0

    authors: Desai D,Ellington MJ,Arnold C,Desai M

    更新日期:2012-12-01 00:00:00

  • Effect of antimycotic agents on the activity of aspartyl proteinases secreted by Candida albicans.

    abstract::The inhibitory effect of human immunodeficiency virus (HIV) proteinase inhibitors amprenavir and saquinavir and antifungal agents terbinafine, ketoconazole, amphotericin B and ciclopiroxolamine on aspartyl proteinases (Saps) secreted by Candida albicans was tested in an in vitro spectophotometric assay. As expected, b...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.05048-0

    authors: Schaller M,Krnjaic N,Niewerth M,Hamm G,Hube B,Korting HC

    更新日期:2003-03-01 00:00:00