Depletion of lymphocytes, but not neutrophils, via apoptosis in a murine model of Vibrio vulnificus infection.

Abstract:

:Vibrio vulnificus causes severe sepsis in humans. There are several reports about the relationship between host immunity and bacterial growth in V. vulnificus infection. However, the effect on leukocytes of V. vulnificus infection in vivo has not been elucidated. A murine model of V. vulnificus infection was used to investigate its effects on leukocytes in this study. Bacteria were recovered from the blood of mice 3 h after subcutaneous injection in the right lower flank. They were detected in 87.5 % (n = 7/8) of mice at 6 h, but this value decreased to 12.5 % (n = 1/8) at 12 h. In contrast, the number of lymphocytes in peripheral blood had already started to decrease at 3 h, and reached a minimum at 6-9 h post-inoculation. Typical DNA laddering, a hallmark of apoptosis, was also detected in thymocytes and splenocytes at 6 and 9 h, and showed a tendency to disappear by 12 h. Although the number of lymphocytes decreased in the model, the numbers of neutrophils did not. These results suggested that V. vulnificus has selective cytotoxicity for lymphocytes in peripheral blood in vivo, and the lymphocyte depletion was probably associated with apoptosis in vivo.

journal_name

J Med Microbiol

authors

Kashimoto T,Ueno S,Hayashi H,Hanajima M,Yoshioka K,Yoshida K,Mutoh K,Susa N

doi

10.1099/jmm.0.45861-0

keywords:

subject

Has Abstract

pub_date

2005-01-01 00:00:00

pages

15-22

issue

Pt 1

eissn

0022-2615

issn

1473-5644

journal_volume

54

pub_type

杂志文章
  • Streptococcal serotypes newly associated with epidemic post-streptococcal acute glomerulonephritis.

    abstract::The increasing incidence of reported scabies in Trinidad, from 24.2/100,000 population in 1984 to 59.5/100,000 in 1985, led to a careful monitoring in 1986 of all patients with post-streptococcal acute glomerulonephritis (PSAGN). There were 181 cases of PSAGN; 84 beta-haemolytic streptococcal isolates from 72 patients...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-32-2-111

    authors: Reid HF,Bassett DC,Gaworzewska E,Colman G,Poon-King T

    更新日期:1990-06-01 00:00:00

  • Antibiofilm efficacy of honey and bee-derived defensin-1 on multispecies wound biofilm.

    abstract::Many clinically relevant biofilms are polymicrobial. Examining the effect of antimicrobials in a multispecies biofilm consortium is of great clinical importance. The goal of this study was to investigate the effect of different honey types against bacterial wound pathogens grown in multispecies biofilm and to test the...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000227

    authors: Sojka M,Valachova I,Bucekova M,Majtan J

    更新日期:2016-04-01 00:00:00

  • Resistance to desiccation and skin fatty acids in outbreak strains of methicillin-resistant Staphylococcus aureus.

    abstract::Resistance to desiccation and to skin fatty acids was measured in three groups of methicillin-resistant Staphylococcus aureus (MRSA) strains and a group of control strains. Organisms from a large outbreak on a special care baby unit (SCBU), where MRSA had been isolated from staff hands but not from the environment, we...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-36-1-56

    authors: Farrington M,Brenwald N,Haines D,Walpole E

    更新日期:1992-01-01 00:00:00

  • Utility of real-time amplification of selected 16S rRNA gene sequences as a tool for detection and identification of microbial signatures directly from clinical samples.

    abstract::The potential of incorporating a real-time PCR for amplification and detection of 16S rRNA gene signatures directly from clinical samples was assessed as a tool for diagnostics. Universal PCR primers spanning short variable regions (~500 bp) were optimized for real-time PCR and tested in comparison with a longer fragm...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.041764-0

    authors: Edwards KJ,Logan JMJ,Langham S,Swift C,Gharbia SE

    更新日期:2012-05-01 00:00:00

  • Group C Streptococcus dysgalactiae subsp. equisimilis in south-east Brazil: genetic diversity, resistance profile and the first report of human and equine isolates belonging to the same multilocus sequence typing lineage.

    abstract::Streptococcus dysgalactiae subsp. equisimilis (SDSE) isolates are the most common group C streptococci in humans and reports of invasive infections associated with SDSE have been increasing. Molecular epidemiology studies are an important strategy to trace the emergence and spread of possible well-fit bacterial pathog...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000052

    authors: Silva LG,Genteluci GL,Corrêa de Mattos M,Glatthardt T,Sá Figueiredo AM,Ferreira-Carvalho BT

    更新日期:2015-05-01 00:00:00

  • Immunological response to a strain of Staphylococcus epidermidis in the rabbit: production of protective antibody.

    abstract::A rabbit was immunised with a heat-killed vaccine prepared from strain 1142 of Staphylococcus epidermidis. The HA titres of the antisera against a surface-polysaccharide antigen extracted from the homologous strain were highest in the 2nd-4th weeks after immunisation and subsequently declined. Only a minor amount of 2...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-11-4-371

    authors: Yoshida K,Ichiman Y

    更新日期:1978-11-01 00:00:00

  • Molecular identification of microsporidian species in patients with epithelial keratitis.

    abstract::Introduction. Ocular microsporidiosis is a significant emerging infectious disease reported in immunocompromised patients and immunocompetent persons throughout the world.Aim. To identify the pathogens responsible for human keratitis, via corneal scrapings.Methodology. Thirty-three hospitalized patients with epithelia...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001164

    authors: Sanpool O,Thanathanee O,Laummuanwai P,Maleewong W,Intapan PM

    更新日期:2020-03-01 00:00:00

  • Xpert MRSA screening in surgical patient flow; time for a rethink for hub-and-spoke laboratory models?

    abstract::The move towards pathology networks and hub-and-spoke models of medical laboratory service provision has significantly changed the flow of samples, and the impact of results on patients, over recent years. At the same time advances in technology, including rapid, simple to use molecular platforms, are changing the way...

    journal_title:Journal of medical microbiology

    pub_type: 信件

    doi:10.1099/jmm.0.000919

    authors: Rabinowicz S,O'Hare M,Moore LSP,Mughal N

    更新日期:2019-03-01 00:00:00

  • Prevalence of childhood diarrhoea-associated Escherichia coli in Thailand.

    abstract::Escherichia coli isolates (n=2629) were collected between 1996 and 2000 from 2100 Thai children less than 12 years of age with acute diarrhoea. Enterotoxigenic (ETEC), enteroinvasive (EIEC), Shiga-toxin-producing (STEC), enteropathogenic (EPEC) and enteroaggregative (EAEC) E. coli were identified by their virulence ma...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.05413-0

    authors: Ratchtrachenchai OA,Subpasu S,Hayashi H,Ba-Thein W

    更新日期:2004-03-01 00:00:00

  • Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates.

    abstract::The family of leptospiral immunoglobulin-like (lig) genes comprises ligA, ligB and ligC. This study used PCR to demonstrate the presence of lig genes among serovars from a collection of leptospiral strains and clinical isolates. Whilst ligA and ligC appeared to be present in a limited number of pathogenic serovars, th...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.009175-0

    authors: Cerqueira GM,McBride AJA,Picardeau M,Ribeiro SG,Moreira ÂN,Morel V,Reis MG,Ko AI,Dellagostin OA

    更新日期:2009-09-01 00:00:00

  • The establishment and persistence of Ureaplasma urealyticum in oestradiol-treated female mice.

    abstract::Administration of oestradiol to three strains of female mice induced the oestrous phase of the reproductive cycle in which there are few or no polymorphonuclear leucocytes (PMNL) in vaginal smears. This treatment rendered the mice susceptible to genital tract colonisation by serotype 8 of Ureaplasma urealyticum, inocu...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-29-2-111

    authors: Furr PM,Taylor-Robinson D

    更新日期:1989-06-01 00:00:00

  • Virulence properties of Escherichia coli faecal strains isolated in Poland from healthy children and strains belonging to serogroups O18, O26, O44, O86, O126 and O127 isolated from children with diarrhoea.

    abstract::Four hundred and twenty-seven Escherichia coli isolates from 427 cases of infantile diarrhoea in Poland, belonging to serogroups O18, O26, O44, O86, O126 and O127 and 150 E. coli isolates from 52 healthy children were examined for selected virulence properties. The presence of the plasmid pAA, a plasmid encoding enter...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-51-7-548

    authors: Paciorek J

    更新日期:2002-07-01 00:00:00

  • Improved serodiagnosis of Campylobacter jejuni infections using recombinant antigens.

    abstract::Campylobacter jejuni is a frequent cause of infectious diarrhoea and is increasingly recognized as a trigger for late-onset complications. The poor standardization of commonly used serological tests might explain the conflicting results regarding the frequency of antecedent C. jejuni infections in defined patient grou...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46040-0

    authors: Schmidt-Ott R,Brass F,Scholz C,Werner C,Groß U

    更新日期:2005-08-01 00:00:00

  • Studies on the relationship between adhesive activity and haemagglutination by Helicobacter pylori.

    abstract::The adhesion of Helicobacter pylori to gastric carcinoma cells (MKN45, KatoIII and MKN28) and Intestine-407 cells was tested by flow cytometric analysis. The mean adhesion rates of H. pylori strains to MKN45, KatoIII and Intestine-407 cells were 90.5, 42.7 and 15.1%, respectively. There was no statistical correlation ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-46-2-117

    authors: Osaki T,Yamaguchi H,Taguchi H,Kumada J,Ogata S,Kamiya S

    更新日期:1997-02-01 00:00:00

  • Evaluation of risk factors for colistin resistance among uropathogenic isolates of Escherichia coli and Klebsiella pneumoniae: a case-control study.

    abstract:INTRODUCTION:The last few years have seen the emergence of multi-drug resistant (MDR) Gram-negative infections, which are associated with high morbidity and mortality. The indiscriminate use of colistin has led to the development of resistance, which can be diagnosed effectively by broth microdilution. Studies from Ind...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000986

    authors: Kaza P,Mahindroo J,Veeraraghavan B,Mavuduru RS,Mohan B,Taneja N

    更新日期:2019-06-01 00:00:00

  • Prospective evaluation of BDProbeTec strand displacement amplification (SDA) system for diagnosis of tuberculosis in non-respiratory and respiratory samples.

    abstract::Nucleic acid amplification techniques (NAATs) have been demonstrated to make significant improvements in the diagnosis of tuberculosis (TB), particularly in the time to diagnosis and the diagnosis of smear-negative TB. The BD ProbeTec strand displacement amplification (SDA) system for the diagnosis of pulmonary and no...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45780-0

    authors: McHugh TD,Pope CF,Ling CL,Patel S,Billington OJ,Gosling RD,Lipman MC,Gillespie SH

    更新日期:2004-12-01 00:00:00

  • Patterns of Candida biofilm on intrauterine devices.

    abstract::Biofilms are colonies of microbial cells encased in a self-produced organic polymeric matrix and represent a common mode of microbial growth. Microbes growing as biofilm are highly resistant to commonly used antimicrobial drugs. We aimed to screen and characterize biofilm formation by different isolates of Candida on ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000042

    authors: Zahran KM,Agban MN,Ahmed SH,Hassan EA,Sabet MA

    更新日期:2015-04-01 00:00:00

  • Fifteen Streptococcus suis serotypes identified by multiplex PCR.

    abstract::A multiplex PCR was developed to detect 15 serotypes of Streptococcus suis. This multiplex PCR was separated into two reaction sets. The first set identified nine serotypes (serotypes 1/2, 1, 2, 3, 4, 7, 9, 14 and 16) and the second set identified six serotypes (serotypes 5, 8, 10, 19, 23 and 25). This assay correctly...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.048587-0

    authors: Kerdsin A,Dejsirilert S,Akeda Y,Sekizaki T,Hamada S,Gottschalk M,Oishi K

    更新日期:2012-12-01 00:00:00

  • Spread of the Brazilian epidemic clone of a multiresistant MRSA in two cities in Argentina.

    abstract::Methicillin-resistant Staphylococcus aureus (MRSA) is recognised as an important cause of nosocomial infection. The spread of some MRSA epidemic clones is well documented. In Brazil, and more recently in Portugal, a considerable number of hospital infections has been caused by a unique multiresistant MRSA clone design...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-49-2-187

    authors: Coimbra MVDS,Teixeira LA,Ramos RLB,Predari SC,Castello L,Famiglietti A,Vay C,Klan L,Figueiredo AMS

    更新日期:2000-02-01 00:00:00

  • Bacterial adhesion and biofilm formation on yttria-stabilized, tetragonal zirconia and titanium oral implant materials with low surface roughness - an in situ study.

    abstract::Bacterially-driven mucosal inflammation and the development of periimplantitis can lead to oral implant failure. In this study, initial bacterial adhesion after 2 h, and biofilm formation after 1 day and 3 days, were analysed in situ on novel 3 mol % yttria-stabilized tetragonal zirconia polycrystal samples, as well a...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000267

    authors: Al-Ahmad A,Karygianni L,Schulze Wartenhorst M,Bächle M,Hellwig E,Follo M,Vach K,Han JS

    更新日期:2016-07-01 00:00:00

  • Nontuberculous mycobacterial and Nocardia infections mimicking pulmonary tuberculosis: a retrospective study of a general hospital patient population in China.

    abstract::Purpose. In this study, we differentiated between tuberculosis (TB) and infections caused by nontuberculous mycobacteria (NTM) or Nocardia in a tertiary general hospital in China. Differences in clinical manifestations and factors between respiratory infections associated with these organisms were also investigated.Me...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000961

    authors: Dong G,Chu P,Guo J,Xie Y,Lu J

    更新日期:2020-09-01 00:00:00

  • Differential adhesion and invasion by Staphylococcus aureus of epithelial cells derived from different anatomical sites.

    abstract::Staphylococcus aureus can invade epithelial cells, and the host-cell receptor α(5)β(1) integrin is thought to mediate this process. The aim of this study was to investigate S. aureus invasion of epithelial cell lines derived from oral (H357), skin (UP) and nasopharyngeal (Detroit 562) sites and to determine whether an...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.049650-0

    authors: Ridley RA,Douglas I,Whawell SA

    更新日期:2012-12-01 00:00:00

  • Inhibition by amoeba-specific antiserum and by cytochalasin B of the cytopathogenicity of Naegleria fowleri in mouse embryo-cell cultures.

    abstract::Inhibitors of trophozoite motility and phagocytosis were used to investigate the mechanism of Naegleria fowleri cytopathogenicity in mouse-embryo (ME)-cell cultures. Amoebae that were immobilised and agglutinated by specific antiserum exhibited no cytopathic activity, although they remained alive and were in constant ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-12-3-355

    authors: Brown T

    更新日期:1979-08-01 00:00:00

  • Clioquinol is a promising preventive morphological switching compound in the treatment of Candida infections linked to the use of intrauterine devices.

    abstract:PURPOSE:Candida biofilm infections are frequently linked to the use of biomaterials and are of clinical significance because they are commonly resistant to antifungals. Clioquinol is an antiseptic drug and is effective against multidrug-resistant Candida. We investigated the effect of clioquinol and two other 8-hydroxy...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000850

    authors: Pippi B,Machado GDRM,Bergamo VZ,Alves RJ,Andrade SF,Fuentefria AM

    更新日期:2018-11-01 00:00:00

  • Effect of Bacteroides fragilis cellular components on chemotactic activity of polymorphonuclear leukocytes towards Escherichia coli.

    abstract::Chemotaxis of polymorphonuclear leukocytes (PMNL) in response to cell components of Bacteroides fragilis alone or in combination with Escherichia coli was evaluated. E. coli produced much more powerful chemotactic factors than B. fragilis. The culture filtrate (CF), outer membrane (OM) preparation, and lipopolysacchar...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-24-2-119

    authors: Namavar F,Verweij-Van Vught AM,Bal M,MacLaren DM

    更新日期:1987-09-01 00:00:00

  • The effects of iclaprim on exotoxin production in methicillin-resistant and vancomycin-intermediate Staphylococcus aureus.

    abstract:PURPOSE:Extracellular protein toxins contribute to the pathogenesis of Staphylococcus aureus infections. The present study compared the effects of iclaprim and trimethoprim - two folic acid synthesis inhibitors - with nafcillin and vancomycin on production of Panton-Valentine leukocidin (PVL), alpha haemolysin (AH) and...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000929

    authors: Bryant AE,Gomi S,Katahira E,Huang DB,Stevens DL

    更新日期:2019-03-01 00:00:00

  • Evaluation of the polymerase chain reaction for early diagnosis of leptospirosis.

    abstract::Early diagnosis of leptospirosis is important because severe leptospiral infection can run a fulminant course. The polymerase chain reaction (PCR) was evaluated for the detection of leptospires in clinical samples from patients with acute leptospiral infection. Blood and urine samples from 71 patients with leptospiros...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-43-2-110

    authors: Brown PD,Gravekamp C,Carrington DG,van de Kemp H,Hartskeerl RA,Edwards CN,Everard CO,Terpstra WJ,Levett PN

    更新日期:1995-08-01 00:00:00

  • Differentiation of thermolysins and serralysins by monoclonal antibodies.

    abstract::Two monoclonal antibodies (MAbs) to a 36-kDa extracellular metalloprotease (PSCP) from Burkholderia (Pseudomonas) cepacia were found to react with thermolysin, Pseudomonas aeruginosa elastase, alkaline protease (Apr) and LasA, Serratia marcescens protease (SMP), Aeromonas hydrophila protease (AhP), and both the lethal...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-45-3-219

    authors: Kooi C,Sokol PA

    更新日期:1996-09-01 00:00:00

  • The effects of ethyleneglycoltetra-acetic acid on bactericidal activity of human serum against Escherichia coli.

    abstract::The effects of ethyleneglycoltetra-acetic acid (EGTA) and EGTA + magnesium (MgEGTA) on the viable counts of 10 strains of Escherichia coli O6 have been studied in normal human serum (NHS), heat-inactivated serum (HIS) and in culture media with and without the addition of a beta-lactam antibiotic. The addition of EGTA ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-14-2-195

    authors: Roberts AP,Phillips R

    更新日期:1981-05-01 00:00:00

  • Isolation of Carnobacterium sp. from a human blood culture.

    abstract::Carnobacterium species have been isolated from the environment and are not regarded as human pathogens, although they are known to cause disease in fish. Only two reports describing isolation of Carnobacterium species from human pus were found in the literature. We report what we believe to be the first isolation of C...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.016808-0

    authors: Hoenigl M,Grisold AJ,Valentin T,Leitner E,Zarfel G,Renner H,Krause R

    更新日期:2010-04-01 00:00:00