Immunological response to a strain of Staphylococcus epidermidis in the rabbit: production of protective antibody.

Abstract:

:A rabbit was immunised with a heat-killed vaccine prepared from strain 1142 of Staphylococcus epidermidis. The HA titres of the antisera against a surface-polysaccharide antigen extracted from the homologous strain were highest in the 2nd-4th weeks after immunisation and subsequently declined. Only a minor amount of 2-ME-resistant antibody was formed, and this at a late state of the reaction. After a booster injection of the vaccine at the 10th week, there was a significantly greater HA-antibody response and a greater amount of 2-ME-resistant antibody was formed. The relative passive-protective activity of the sera in mice corresponded to their content of 2-ME-sensitive HA antibody. The protective activity of the sera was 2-ME sensitive and was not removed by absorption with anti-rabbit IgG goat serum. Further, 280 microgram of IgM-rich serum fraction obtained by sucrose-density-gradient ultracentrifugation passively protected against homologous challenge infection in mice, but even 910 microgram of IgG-rich fraction did not. These results indicated that the protective antibody was of the IgM class.

journal_name

J Med Microbiol

authors

Yoshida K,Ichiman Y

doi

10.1099/00222615-11-4-371

subject

Has Abstract

pub_date

1978-11-01 00:00:00

pages

371-7

issue

4

eissn

0022-2615

issn

1473-5644

journal_volume

11

pub_type

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