Mechanisms of chloramphenicol resistance in Haemophilus influenzae in the United Kingdom.

Abstract:

:Of 2458 isolates of Haemophilus influenzae examined in a recent British survey, 42 were resistant to chloramphenicol. Two resistant isolates were of type b and 40 were non-capsulate. Spectrophotometric assay showed that all the resistant isolates produced chloramphenicol acetyltransferase (CAT). CAT activity did not increase following growth on heated blood agar containing chloramphenicol 2 mg/L but was reduced by 84-98% when extracts were treated for 30 min with 5', 5'-dithiobis-2-nitrobenzoate. These data suggest that H. influenzae CATs resemble the Type-II CATs produced by enterobacteria. Extrachromosomal DNA was detected in five only of the 42 resistant isolates and cured derivatives of two plasmid-containing strains retained their chloramphenicol resistance. These results suggest that the CAT gene is located on the chromosome.

journal_name

J Med Microbiol

authors

Powell M,Livermore DM

doi

10.1099/00222615-27-2-89

subject

Has Abstract

pub_date

1988-10-01 00:00:00

pages

89-93

issue

2

eissn

0022-2615

issn

1473-5644

journal_volume

27

pub_type

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