Rapid discrimination of Staphylococcus epidermidis genotypes in a routine clinical microbiological laboratory using single nucleotide polymorphisms in housekeeping genes.

abstract：:A pathogenic vancomycin-resistant Staphylococcus aureus (VRSA) isolate (MIC > or =64 microg ml(-1)) was obtained from a Kolkata hospital in June 2005. Species identification was confirmed by Gram staining, standard biochemical tests and PCR amplification of the nuc gene, which encodes the thermostable nuclease that is...

journal_title：Journal of medical microbiology

authors： Saha B,Singh AK,Ghosh A,Bal M

更新日期：2008-01-01 00:00:00

abstract：:The bactericidal action of non-immune whole blood on Proteus mirabilis was increased when the bacteria contained the R-plasmid RP1. This effect was due mainly to increased phagocytosis. Iron-depleted stationary-phase cells were more sensitive than carbon-depleted cells. The contribution of serum was usually negligible...

journal_title：Journal of medical microbiology

authors： Onaolapo JA,Klemperer RM

更新日期：1987-11-01 00:00:00

abstract：:Bacteroides gracilis is a gram-negative anaerobic bacillus which requires formate and fumarate for growth; it has been implicated in periodontal disease and serious infections of the head and neck. In this study, Bacteroides ureolyticus (NTU) medium was tested for its ability to allow the growth of B. gracilis and oth...

journal_title：Journal of medical microbiology

authors： Wade WG,Bishop PA,Slayne MA

更新日期：1991-11-01 00:00:00

abstract：:Clostridium difficile is the most common cause of antibiotic-associated diarrhoea. Antibiotics are presumed to disturb the normal intestinal microbiota, leading to depletion of the barrier effect and colonization by pathogenic bacteria. This first step of infection includes adherence to epithelial cells. We investigat...

journal_title：Journal of medical microbiology

authors： Denève C,Deloménie C,Barc MC,Collignon A,Janoir C

更新日期：2008-06-01 00:00:00

abstract：:Subtractive hybridisation was used to screen for and identify conserved DNA sequences associated with clinical, clonal populations of Staphylococcus aureus. DNA from S. aureus strain ISP8 (a clinical isolate) was digested with TaqI and hybridised with randomly fragmented pooled DNA from 10 non-clinical (community) iso...

journal_title：Journal of medical microbiology

authors： el-Adhami WA,Stewart PR,Matthaei KI

更新日期：1997-12-01 00:00:00

abstract：PURPOSE:We resequenced the genome of Clostridium difficile 630Δerm (DSM 28645), a model strain commonly used for the generation of insertion mutants. METHODOLOGY:The genome sequence was obtained by a combination of single-molecule real-timeand Illumina sequencing technology. RESULTS:Detailed manual curation and compa...

journal_title：Journal of medical microbiology

authors： Dannheim H,Riedel T,Neumann-Schaal M,Bunk B,Schober I,Spröer C,Chibani CM,Gronow S,Liesegang H,Overmann J,Schomburg D

更新日期：2017-03-01 00:00:00

abstract：:A co-agglutination (Co-A) test with Staphylococcus aureus (Cowan's strain I) bearing protein A coated with specific hydatid antibodies, was used to demonstrate circulating hydatid antigen in serum for the diagnosis of hydatid disease. The test had a sensitivity of 95% and specificity of 89% in detecting hydatid antige...

journal_title：Journal of medical microbiology

authors： Shariff M,Parija SC

更新日期：1993-06-01 00:00:00

abstract：:The performance of the Fungitell assay was investigated in 100 patients with haematological malignancy undergoing chemotherapy who developed antibiotic-unresponsive neutropenic fever (AUNF). Serum beta-D-glucan (BG) concentrations were significantly elevated on the first day of AUNF and all subsequent alternate days t...

journal_title：Journal of medical microbiology

authors： Ellis M,Al-Ramadi B,Finkelman M,Hedstrom U,Kristensen J,Ali-Zadeh H,Klingspor L

更新日期：2008-03-01 00:00:00

abstract：:PCR has proved superior to conventional blood culture for diagnosing bacteraemia in the presence of antibiotics. Nevertheless, even PCR might yield false-negative results if the template DNA were to be cleaved by serum DNAases after antibiotics had induced bacterial death. To evaluate the cleavage of bacterial templat...

journal_title：Journal of medical microbiology

authors： Heininger A,Ulrich M,Priebe G,Unertl K,Müller-Schauenburg A,Botzenhart K,Döring G

更新日期：2001-03-01 00:00:00

abstract：:The aim of this study was to explore the epidemiological and molecular characteristics of Streptococcus pyogenes in children from different cities in mainland China who were diagnosed with scarlet fever, impetigo and pharyngitis, as well as to detect asymptomatic carriers, between 2005 and 2008, and to compare the res...

journal_title：Journal of medical microbiology

authors： Liang Y,Liu X,Chang H,Ji L,Huang G,Fu Z,Zheng Y,Wang L,Li C,Shen Y,Yu S,Yao K,Ma L,Shen X,Yang Y

更新日期：2012-07-01 00:00:00

abstract：:Episodes of cholera stemming from indigenous Vibrio cholerae strains in Australia are mainly associated with environmental sources. In the present study, 10 V. cholerae O1 strains of Australian origin were characterized. All of the strains were serogroup O1 and their conventional phenotypic traits categorized them as ...

journal_title：Journal of medical microbiology

authors： Safa A,Bhuiyan NA,Murphy D,Bates J,Nusrin S,Kong RYC,Chongsanguan M,Chaicumpa W,Nair GB

更新日期：2009-01-01 00:00:00

abstract：PURPOSE:Penicillium is the most common mould isolated in housing. Penicillium chrysogenum is the only species tested by prick test or serology for allergic patients. The American Institute of Medicine has accepted Penicillium as an aetiological agent of rhinitis in children and adults and as an asthma agent in children...

journal_title：Journal of medical microbiology

authors： Reboux G,Rocchi S,Vacheyrou M,Millon L

更新日期：2019-05-01 00:00:00

abstract：:Sorbarod biofilms were investigated for their suitability in establishing continuous culture biofilms for the study of bacterial vaginosis. Two important organisms in the condition, Gardnerella vaginalis and Lactobacillus acidophilus, were studied. In contrast to growth in broth culture, both organisms were maintained...

journal_title：Journal of medical microbiology

authors： Muli FW,Struthers JK

更新日期：1998-05-01 00:00:00

abstract：:The relationship between Streptococcus constellatus, one of the species of the "Streptococcus milleri group", and Prevotella intermedia was studied in a model of pneumonia in mice and in vitro to elucidate mechanisms of pathogenicity in "S. milleri group"-associated pulmonary infection. Acute pneumonia with or without...

journal_title：Journal of medical microbiology

authors： Shinzato T,Saito A

更新日期：1994-02-01 00:00:00

abstract：:Enteropathogenic Escherichia coli (EPEC) is an important aetiological agent of persistent infantile diarrhoea. EPEC pathogenicity is not mediated through known toxins and the role played by outer-membrane proteins (OMPs) in the initial adherence of the bacterium, intimate attachment to epithelial cells and ultimately ...

journal_title：Journal of medical microbiology

authors： Kumar SS,Sankaran K,Haigh R,Williams PH,Balakrishnan A

更新日期：2001-07-01 00:00:00

abstract：:The cell-wall characteristics of Gardnerella vaginalis grown in conventional and biofilm systems were studied by electron microscopy. The gram-positive nature of the cell wall was confirmed. Novel cell-wall particles which appeared to be associated with cell division were also identified, particularly in organisms of ...

journal_title：Journal of medical microbiology

authors： Muli FW,Struthers JK,Tarpey PA

更新日期：1999-02-01 00:00:00

abstract：:Introduction. In May-June 2018, an outbreak of campylobacteriosis involved students and school staff from kindergartens and primary schools in Pescara, southern Italy.Aim. We present details of the epidemiological and microbiological investigation, and the findings of the analytical study, as well as the implemented c...

journal_title：Journal of medical microbiology

authors： Sorgentone S,Busani L,Calistri P,Robuffo G,Bellino S,Acciari V,Ferri M,Graziani C,Antoci S,Lodi F,Alfonsi V,Cammà C,Fazii P,Andrianou X,Cito F,Lombardi G,Centorotola G,D'Amario M,D'Alterio N,Savini V,De Massis F,

更新日期：2021-01-21 00:00:00

abstract：:Amplification and sequence analysis of the 16S rRNA gene can be applied to detect and identify bacteria in clinical samples. We examined 75 clinical samples (17 culture-positive, 58 culture-negative) prospectively by two different PCR protocols, amplifying either a single fragment (1343 bp) or two fragments (762/598 b...

journal_title：Journal of medical microbiology

authors： Jenkins C,Ling CL,Ciesielczuk HL,Lockwood J,Hopkins S,McHugh TD,Gillespie SH,Kibbler CC

更新日期：2012-04-01 00:00:00

abstract：:Invasive infections caused by Brevundimonas vesicularis are very rare in humans. We experienced an unusual case of liver abscess due to B. vesicularis in an immunocompetent young male. The patient was successfully treated by liver abscess drainage and with antimicrobial therapy of ceftriaxone followed by ampicillin/su...

journal_title：Journal of medical microbiology

authors： Yoo SH,Kim MJ,Roh KH,Kim SH,Park DW,Sohn JW,Yoon YK

更新日期：2012-10-01 00:00:00

abstract：:EDTA-released outer-membrane antigen complexes were prepared from 172 laboratory and reference strains and 43 fresh clinical or faecal isolates representing 20 species or subspecies of Bacteroides, together with 13 species of other genera. These antigens were titrated against antisera to whole, live cells of 21 specie...

journal_title：Journal of medical microbiology

authors： Poxton IR,Brown R,Collee JG

更新日期：1982-05-01 00:00:00

abstract：:The ability of cefotaxime, ciprofloxacin, piperacillin and tobramycin to cause release of endotoxin was examined in vitro with cultures of Enterobacter cloacae and Escherichia coli. Endotoxin was measured by a quantitative limulus amoebocyte lysate assay and its presence was confirmed by silver staining of the lipopol...

journal_title：Journal of medical microbiology

authors： Crosby HA,Bion JF,Penn CW,Elliott TS

更新日期：1994-01-01 00:00:00

abstract：:Phenotypic loss of protein A production was tested in six methicillin-resistant (McR) Staphylococcus aureus (MRSA) isolates and their isogenic methicillin-sensitive (McS) variants by a radiolabelled IgG-binding assay with washed cells and by Western blotting of supernates prepared from lysed washed cells. Genomic DNA ...

journal_title：Journal of medical microbiology

authors： Poston SM,Glancey GR,Wyatt JE,Hogan T,Foster TJ

更新日期：1993-12-01 00:00:00

abstract：:Neisseria sicca is rarely associated with clinical infections and to the authors' knowledge this organism has not been reported as a causative agent of infected biloma (liver abscess). A case of a diabetic man with infected biloma due to N. sicca after repeated transcatheter arterial embolization for hepatocellular ca...

journal_title：Journal of medical microbiology

authors： Chung HC,Teng LJ,Hsueh PR

更新日期：2007-11-01 00:00:00

abstract：:Heated spores (80 degrees C, 10 min) of Clostridium butyricum strain CB 1002 isolated from a fatal case of necrotising enterocolitis in a human neonate were inoculated into ligated intestinal loops prepared in young conventional guinea pigs. Necropsy findings 18 h later included congestion, patchy haemorrhage of the i...

journal_title：Journal of medical microbiology

authors： Popoff MR,Ravisse P

更新日期：1985-06-01 00:00:00

abstract：:The present study aimed to evaluate the role of biofilm morphology, matrix content and surface hydrophobicity in the biofilm-forming capacity of Candida albicans and non-albicans Candida (NAC) spp. Biofilm formation was determined by microtitre plate assay and bright-field and scanning electron microscopy. The matrix ...

journal_title：Journal of medical microbiology

authors： Kumari A,Mankotia S,Chaubey B,Luthra M,Singh R

更新日期：2018-06-01 00:00:00

abstract：:Clostridium botulinum type A toxin is the most prevalent cause of naturally occurring outbreaks of human botulism in the world. The active dichain neurotoxin molecule is composed of a heavy chain (H-chain) of ~100 kDa with the carboxy-terminal end consisting of a receptor-binding (HC) domain, while the amino-terminal ...

journal_title：Journal of medical microbiology

authors： Jones RGA,Marks JD

更新日期：2013-06-01 00:00:00

abstract：:Yersinia pseudotuberculosis was examined for its haemagglutinating activity and adherence to cultured epithelial cells (HEp-2) in relation to possession of a virulence (VW) plasmid and to growth conditions. VW-lacking (VW-) bacteria were isolated from ten VW+ strains of each serovar which, after they were grown on CFA...

journal_title：Journal of medical microbiology

authors： Kawaoka Y,Mitani T,Hoshina K,Otsuki K,Tsubokura M

更新日期：1988-03-01 00:00:00

abstract：:In tests of bactericidal action against H. influenzae type b strains isolated from patients with meningitis, chloramphenicol was found to be far more reliable than ampicillin in dealing with large inocula, and more rapidly effective against both large and relatively small inocula. These findings provide a laboratory e...

journal_title：Journal of medical microbiology

authors： Turk DC

更新日期：1977-02-01 00:00:00

abstract：:Rapid identification of isolates in positive blood cultures are of great importance to secure correct treatment of septicaemic patients. As antimicrobial resistance is increasing, rapid detection of resistance is crucial. Carbapenem resistance in Bacteroides fragilis associated with cfiA-encoded class B metallo-beta-l...

journal_title：Journal of medical microbiology

authors： Johansson Å,Nagy E,Sóki J

更新日期：2014-08-01 00:00:00

abstract：:The unpredictable characteristics of meningococcal disease (MD) make outbreaks complicated to monitor and consequently lead to high levels of public anxiety. Traditional molecular techniques have been utilized in order to understand better the epidemiology of MD, but some have disadvantages such as being highly specia...

journal_title：Journal of medical microbiology

authors： Diggle MA,Bell CM,Clarke SC

更新日期：2003-06-01 00:00:00