Detection of species-specific and cross-reactive cell-surface antigens of Bacteroides species by an indirect enzyme-linked immunosorbent assay.


:EDTA-released outer-membrane antigen complexes were prepared from 172 laboratory and reference strains and 43 fresh clinical or faecal isolates representing 20 species or subspecies of Bacteroides, together with 13 species of other genera. These antigens were titrated against antisera to whole, live cells of 21 species or subspecies of Bacteroidaceae in an indirect enzyme-linked immunosorbent assay (ELISA). The presence of species-specific antigens was investigated and cross reactions between species were noted. Results showed that a high proportion of the species possess species-specific antigens with little significant cross reactivity. We believe that the ELISA system described here detects antigens that represent the whole cell surface of bacteroides organisms. We have exploited the sensitivity of the system and its quantitative potential to define approaches for those wishing to use serological approaches for either the identification of Bacteroides species or for the titration of serum antibodies in patients with a possible bacteroides infection.


J Med Microbiol


Poxton IR,Brown R,Collee JG




Has Abstract


1982-05-01 00:00:00












  • Replication of Chlamydia trachomatis and C. pneumoniae in the human monocytic cell line U-937.

    abstract::To elucidate whether Chlamydia trachomatis and C. pneumoniae infections occur to a significant extent in monocytes-macrophages, the human monocytic cell line, U-937, was infected with C. trachomatis L2 or C. pneumoniae TW-183. Chlamydial DNA and genus-specific antigens of the lipopolysaccharides (LPS) in epitopes of t...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Numazaki K,Suzuki K,Chiba S

    更新日期:1995-03-01 00:00:00

  • Experimental infection of germ-free mice with hyper-toxigenic enterohaemorrhagic Escherichia coli O157:H7, strain 6.

    abstract::A mouse enterohaemorrhagic Escherichia coli (EHEC) infection model was developed with a combination of germ-free (GF) mice and hyper-toxigenic EHEC (HT-EHEC) O157:H7 strain 6. The HT-EHEC strain 6 produced both Shiga-like toxin (SLT)-1 1.0 microg/mI and SLT-2 8.2 microg/ml in vitro. Eight-week-old germ-free mice were ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Taguchi H,Takahashi M,Yamaguchi H,Osaki T,Komatsu A,Fujioka Y,Kamiya S

    更新日期:2002-04-01 00:00:00

  • Radiometric studies on the use of selective inhibitors in the identification of Mycobacterium spp.

    abstract::Radiometric selective inhibition tests were developed and evaluated for the rapid differentiation of Mycobacterium spp. Both a p-nitrobenzoic acid (PNB) test and a commercially-prepared p-nitro-alpha-acetylamino-beta-hydroxypropiophenone (NAP) test successfully differentiated M. tuberculosis and M. bovis from "atypica...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Collins T,Levett PN

    更新日期:1989-11-01 00:00:00

  • Prospective evaluation of the new chromogenic medium CandiSelect 4 for differentiation and presumptive identification of the major pathogenic Candida species.

    abstract::The rapid identification of pathogenic yeasts is a crucial step in ensuring that effective antifungal treatment is started as early as possible. CandiSelect 4 (CS4; Bio-Rad) is a new chromogenic medium for the isolation of fungi, the direct identification of Candida albicans and the presumptive identification of the m...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Sendid B,François N,Standaert A,Dehecq E,Zerimech F,Camus D,Poulain D

    更新日期:2007-04-01 00:00:00

  • The DNA-binding domain of CaNdt80p is required to activate CDR1 involved in drug resistance in Candida albicans.

    abstract::CaNdt80p, the Candida albicans homologue of the Saccharomyces cerevisiae transcription factor ScNdt80p, has been identified as a positive regulator of CDR1, which encodes an efflux pump involved in drug resistance in C. albicans. To investigate the involvement of the putative DNA-binding domain of CaNdt80p in drug res...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Wang JS,Yang YL,Wu CJ,Ouyang KJ,Tseng KY,Chen CG,Wang H,Lo HJ

    更新日期:2006-10-01 00:00:00

  • Comparative prevalence of superantigenic toxin genes in meticillin-resistant and meticillin-susceptible Staphylococcus aureus isolates.

    abstract::A total of 118 meticillin-resistant Staphylococcus aureus (MRSA) and 140 meticillin-susceptible S. aureus (MSSA) isolates from different patients in the same time period were comprehensively searched using a multiplex PCR for the classical and recently described superantigenic toxin gene family comprising the staphylo...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Hu DL,Omoe K,Inoue F,Kasai T,Yasujima M,Shinagawa K,Nakane A

    更新日期:2008-09-01 00:00:00

  • Virulence of an emerging respiratory pathogen, genus Pandoraea, in vivo and its interactions with lung epithelial cells.

    abstract::Pandoraea species have emerged as opportunistic pathogens among cystic fibrosis (CF) and non-CF patients. Pandoraea pulmonicola is the predominant Pandoraea species among Irish CF patients. The objective of this study was to investigate the pathogenicity and potential mechanisms of virulence of Irish P. pulmonicola is...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Costello A,Herbert G,Fabunmi L,Schaffer K,Kavanagh KA,Caraher EM,Callaghan M,McClean S

    更新日期:2011-03-01 00:00:00

  • Prevalence of Vibrio cholerae with heat-stable enterotoxin (NAG-ST) and cholera toxin genes; restriction fragment length polymorphisms of NAG-ST genes among V. cholerae O serogroups from a major shrimp production area in Thailand.

    abstract::A total of 148 Vibrio cholerae isolates from a major shrimp production area in Southern Thailand were examined by colony hybridisation for genes encoding heat-stable enterotoxin (NAG-ST) and cholera toxin (CT). Only non-O1 V. cholerae strains were found to harbour NAG-ST (14 of 146) whereas no strains hybridised with ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Dalsgaard A,Serichantalergs O,Shimada T,Sethabutr O,Echeverria P

    更新日期:1995-09-01 00:00:00

  • Mapping the genetic diversity within major clonal complexes of meticillin-resistant Staphylococcus aureus utilizing genome-wide fluorescent amplified fragment length polymorphism markers.

    abstract::The genetic diversity between major meticillin-resistant Staphylococcus aureus (MRSA) lineages was probed using fluorescent amplified fragment length polymorphism (FAFLP) as a random genome sampling tool. Genomic DNA was digested with endonucleases BglII and Csp6I and a subset of the restricted fragments were amplifie...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Desai D,Ellington MJ,Arnold C,Desai M

    更新日期:2012-12-01 00:00:00

  • Comparison of detection procedures for Chlamydia trachomatis, including enzyme immunoassays, in a mouse model of genital infection.

    abstract::Two chlamydial enzyme immunoassays, Chlamydiazyme and IDEIA, were evaluated in a mouse model of chlamydial genital-tract infection. The Chlamydiazyme assay and the IDEIA were assessed on specimens from 10 and 11 mice, respectively. The animals were infected with Chlamydia trachomatis, strain SA2f, and the results obta...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Taylor-Robinson D,Tuffrey M

    更新日期:1987-09-01 00:00:00

  • Detection of nephritis strain-associated streptokinase by monoclonal antibodies.

    abstract::Monoclonal antibodies (MAbs) N-59 and RU-1 were produced by immunisation of mice with streptokinase secreted by Streptococcus group A, type 12, strain A374 isolated from a patient with post-streptococcal glomerulonephritis (PSGN) and were characterised by Western blot analysis. MAb N-59 recognised antigenic determinan...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Ohkuni H,Todome Y,Yoshimura K,Yamamoto T,Suzuki H,Yokomuro K,Johnston KH,Zabriskie JB

    更新日期:1991-07-01 00:00:00

  • Oral Candida colonization in HIV-positive women: associated factors and changes following antiretroviral therapy.

    abstract::There is a paucity of information about the factors associated with oral colonization with Candida species and the changes associated with antiretroviral (ARV) therapy. This study investigated the role of ARV therapy and other factors in a study population. Relevant clinical and laboratory information was obtained and...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Owotade FJ,Patel M,Ralephenya TRMD,Vergotine G

    更新日期:2013-01-01 00:00:00

  • Auxotyping of Neisseria gonorrhoeae isolated in the United Kingdom.

    abstract::One hundred and twenty consecutive isolates of Neisseria gonorrhoeae from the County of Avon, UK, were examined by auxotyping and penicillin susceptibility testing. Requirements for proline (Pro), arginine (Arg), hypoxanthine (Hyp), uracil (Ura) and methionine (Met) were determined with a modified Heckels' medium. Pro...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Copley CG,Egglestone SI

    更新日期:1983-08-01 00:00:00

  • Changes in genetic types and population dynamics of Moraxella catarrhalis in hospitalized children are not associated with an exacerbation of existing disease.

    abstract::Pulsed-field gel electrophoresis typing was performed on a retrospective set of 129 Moraxella catarrhalis isolates obtained over a 20 month period from 70 children admitted to, or presenting at, the Erasmus University Medical Center, Rotterdam, The Netherlands. The mean age of the children (at the end of the study) wa...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Hays JP,Eadie K,Verduin CM,Hazelzet J,Verbrugh H,van Belkum A

    更新日期:2003-09-01 00:00:00

  • A collaborative evaluation of a rapid, semi-automated identification system for gram-negative bacilli: the Quantum II BID.

    abstract::The recently-introduced semi-automated bacterial identification system, the Quantum II BID, is designed to identify fermentative and non-fermentative gram-negative bacilli in 4-5 h. The system was evaluated independently by the two participating laboratories. Inter-laboratory reproducibility was determined by testing ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Stevens M,Henrichsen C,Smith M

    更新日期:1986-03-01 00:00:00

  • Balamuthia mandrillaris resistance to hostile conditions.

    abstract::The resistance of Balamuthia mandrillaris to physical, chemical and radiological conditions was tested. Following treatments, viability was determined by culturing amoebae on human brain microvascular endothelial cells for up to 12 days. B. mandrillaris cysts were resistant to repeated freeze-thawing (five times), tem...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Siddiqui R,Ortega-Rivas A,Khan NA

    更新日期:2008-04-01 00:00:00

  • Comparative analysis of phenol-soluble modulin production and Galleria mellonella killing by community-associated and healthcare-associated meticillin-resistant Staphylococcusaureus strains.

    abstract::Community-associated meticillin-resistant Staphylococcus aureus (CA-MRSA) have emerged globally and have been associated with more severe disease than healthcare-associated MRSA (HA-MRSA). The purpose of this study was to determine whether laboratory measures of virulence can distinguish dominant CA-MRSA clones from H...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Boakes E,Marbach H,Lynham S,Ward M,Edgeworth JD,Otter JA

    更新日期:2016-12-01 00:00:00

  • Hyperinvasiveness and increased intercellular spread of Listeria monocytogenes sequence type 1 are independent of listeriolysin S, internalin F and internalin J1.

    abstract:PURPOSE:Listeria monocytogenes is a genetically heterogeneous species, which is divided into evolutionary lineages and clonal complexes (CCs). Not all L. monocytogenes isolates are equally likely to cause disease, with CC1, and in particular sequence type (ST) 1, being the most prevalent complex in human and ruminant i...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Rupp S,Bärtschi M,Frey J,Oevermann A

    更新日期:2017-07-01 00:00:00

  • Haemolysin produced by Vibrio cholerae non-O1 is not enterotoxic.

    abstract::Of 28 isolates of Vibrio cholerae non-O1 (10 from diarrhoeal patients and 18 from environmental sources) examined for haemolytic activity and its correlation, if any, with enterotoxic activity, 24 showed haemolysis. The four non-haemolytic isolates showed haemolysis after consecutive passages through rabbit ileal loop...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Singh DV,Shukla BN,Sanyal SC

    更新日期:1996-07-01 00:00:00

  • Maternal and neonatal sepsis caused by Haemophilus influenzae type d.

    abstract::A 29-year-old pregnant woman was admitted to hospital with signs of sepsis and threatened pre-term labour. The premature neonate also showed signs of sepsis. Haemophilus influenzae biotype III was cultured from a midstream urine sample taken from the mother, maternal placental swabs and neonatal blood cultures. The pl...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Warren S,Tristram S,Bradbury RS

    更新日期:2010-03-01 00:00:00

  • A large food-borne outbreak of campylobacteriosis in kindergartens and primary schools in Pescara, Italy, May-June 2018.

    abstract::Introduction. In May-June 2018, an outbreak of campylobacteriosis involved students and school staff from kindergartens and primary schools in Pescara, southern Italy.Aim. We present details of the epidemiological and microbiological investigation, and the findings of the analytical study, as well as the implemented c...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Sorgentone S,Busani L,Calistri P,Robuffo G,Bellino S,Acciari V,Ferri M,Graziani C,Antoci S,Lodi F,Alfonsi V,Cammà C,Fazii P,Andrianou X,Cito F,Lombardi G,Centorotola G,D'Amario M,D'Alterio N,Savini V,De Massis F,

    更新日期:2021-01-21 00:00:00

  • Experimental rabbit model of meningitis produced by Haemophilus influenzae serotype c.

    abstract::The virulence of Haemophilus influenzae type c when inoculated intracisternally (i.c.) into rabbits was evaluated. Rabbits are relatively resistant to infection with H. influenzae type b, such that inocula of the order of 10(6-9) cfu are required to produce meningitis in this model. In contrast, fatal meningitis was p...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Sulc P,Hengstler B,Krinke G,O'Reilly T,Zak O

    更新日期:1992-05-01 00:00:00

  • Sex differences in antibody- and cell-mediated immune response to rubella re-immunisation.

    abstract::Antibody (AMI) and cell-mediated (CMI) immunity to rubella virus (RV) were evaluated in healthy adolescent males (n = 11) and females (n = 28) undergoing routine reimmunisation with RA27/3 strain RV as a component of measles-mumps-rubella (MMR) vaccine. Blood samples were collected just before and at 2, 4 and 10 weeks...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Mitchell LA

    更新日期:1999-12-01 00:00:00

  • The relationship between opacity factor and M protein in Streptococcus pyogenes.

    abstract::Lancefield acid extracts of Streptococcus pyogenes, type 22 (T12, M22, OF positive) gave good yields of M protein and little opacity factor (OF), but sodium dodecyl sulphate (SDS) extracts contained high titres of OF (greater than 20000) and little M protein. Acid-extracted OF could be separated from M protein by Seph...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Hallas G,Widdowson JP

    更新日期:1983-02-01 00:00:00

  • Identification and characterization of breakthrough contaminants associated with the conventional isolation of Mycobacterium tuberculosis.

    abstract::Breakthrough contamination of tuberculosis (TB) cultures is a problem in that it allows the overgrowth of another bacterium present in the sputum specimen, which can potentially mask the presence of Mycobacterium tuberculosis. The aim of this study was to isolate and characterize the bacterial organisms responsible fo...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: McClean M,Stanley T,Stanley S,Maeda Y,Goldsmith CE,Shepherd R,Millar BC,Dooley JS,Moore JE

    更新日期:2011-09-01 00:00:00

  • Prolonged perturbations of tumour necrosis factor-alpha and interferon-gamma in mice inoculated with Clostridium piliforme.

    abstract::Clostridium piliforme is an obligate intracellular bacterium that causes enterohepatic disease in many animal species. C. piliforme infections are commonly subclinical in laboratory rats and mice, and little is known about host regulation of disease or of the effects of C. piliforme infections on investigations that u...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: VAN Andel RA,Franklin CL,Besch-Williford CL,Hook RR,Riley LK

    更新日期:2000-06-01 00:00:00

  • Production of mouse monoclonal antibodies to Pasteurella multocida type A and the immunological properties of a protective anti-lipopolysaccharide antibody.

    abstract::Eight monoclonal antibodies (MAbs) were produced from mice immunised with whole cells of heat-killed Pasteurella multocida type A which had been cultured under iron-restricted conditions. The MAbs were selected by an enzyme-linked immunosorbent assay (ELISA) in which the antigen consisted of whole bacteria of the immu...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Wijewardana TG,Wilson CF,Gilmour NJ,Poxton IR

    更新日期:1990-12-01 00:00:00

  • Adhesion of clinical and environmental Aeromonas isolates to HEp-2 cells.

    abstract::A total of 63 Aeromonas strains isolated from diarrhoeal faeces or water samples were tested for adhesion to HEp-2 cells. An association between diarrhoea and high level adhesion was observed in that 12 of the 34 faecal isolates and none of the 29 environmental isolates yielded greater than 20 bacteria per HEp-2 cell ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Carrello A,Silburn KA,Budden JR,Chang BJ

    更新日期:1988-05-01 00:00:00

  • Production of extracellular matrix by Candida albicans biofilms.

    abstract::Growth of Candida albicans biofilms and production of extracellular matrix were monitored by dry weight, colorimetric and radioisotope assays, and by scanning electron microscopy. Under static incubation conditions synthesis of matrix material was minimal, but increased dramatically when developing biofilms were subje...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Hawser SP,Baillie GS,Douglas LJ

    更新日期:1998-03-01 00:00:00

  • Identification and typing of pyogenic streptococci by enzyme electrophoretic polymorphism.

    abstract::Polyacrylamide-agarose gel electrophoresis was used to study polymorphism of lactate dehydrogenase (LDH), nucleoside phosphorylase (NSP), phosphoglucose isomerase (PGI), hydroxybutyrate dehydrogenase (HBD), adenylate kinase (ADK) and esterases of 44 strains of Streptococcus pyogenes, 25 group G streptococcal strains, ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Bert F,Picard B,Lambert-Zechovsky N,Goullet P

    更新日期:1995-06-01 00:00:00